HUP0003594A2 - Expression of fungicide-binding polypeptides in plants for producing fungicide tolerance - Google Patents
Expression of fungicide-binding polypeptides in plants for producing fungicide toleranceInfo
- Publication number
- HUP0003594A2 HUP0003594A2 HU0003594A HUP0003594A HUP0003594A2 HU P0003594 A2 HUP0003594 A2 HU P0003594A2 HU 0003594 A HU0003594 A HU 0003594A HU P0003594 A HUP0003594 A HU P0003594A HU P0003594 A2 HUP0003594 A2 HU P0003594A2
- Authority
- HU
- Hungary
- Prior art keywords
- fungicide
- plants
- producing
- antibody
- expression
- Prior art date
Links
- 230000000855 fungicidal effect Effects 0.000 title abstract 5
- 239000000417 fungicide Substances 0.000 title abstract 5
- 229920001184 polypeptide Polymers 0.000 title abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 3
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 3
- 241000196324 Embryophyta Species 0.000 abstract 7
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 241000589155 Agrobacterium tumefaciens Species 0.000 abstract 1
- 101000972350 Bombyx mori Lebocin-4 Proteins 0.000 abstract 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 abstract 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 abstract 1
- 108010076504 Protein Sorting Signals Proteins 0.000 abstract 1
- 241000219094 Vitaceae Species 0.000 abstract 1
- 235000021021 grapes Nutrition 0.000 abstract 1
- 230000014759 maintenance of location Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- -1 methyl-methoxy-imino-α-(o-tolyloxy)-o-tolyl acetate Chemical compound 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
- C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Pharmacology & Pharmacy (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Peptides Or Proteins (AREA)
Abstract
A találmány tárgya eljárás fungicidtoleráns növények előállításáraexogén, fungicidkötő tulajdonságokkal rendelkező polipeptid (előnyösenantitest vagy egyláncú antitestfragmentum) növényekben vagy növényirészekben való expressziójával. Fungicidként például metil-metoxi-imino-a-(o-tolil-oxi)-o-tolil-acetátra (BAS 490F) toleráns növényeketállítanak elő úgy, hogy ezen fungicidet kötni képes antitestetexpresszálnak a növényben mag- vagy levélspecifikusan. Előszörelőállítanak egy expressziós kazettát, amely többek között promotert(előnyösen CaMV-35S promotert), LeB4 szignálpeptidet, a kifejezendőgént és ER retenciós szignált (hogy a felhalmozódott fehérje az ER-benlokalizálódjon) tartalmaz. Azután az expressziós kazettát rekombinánsvektorba (például pBin19-be) inszertálják, amelyet Agrobacteriumtumefaciensbe visznek, ezt azután ismert módon lehet növények,különösen kultúrnövények, például szőlő transzformálására alkalmazni.A találmány szerinti fungicidet (BAS 490F) fitopatogén gombákleküzdésére alkalmazzák transzgenikus, fungicidtoleráns, azaz afungicidet kötni képes polipeptidet vagy antitestet termelőkultúrnövényeken. ÓThe subject of the invention is a method for producing fungicide-tolerant plants by expressing an exogenous polypeptide (preferably antibody or single-chain antibody fragment) with fungicide-binding properties in plants or plant parts. As a fungicide, for example, plants tolerant to methyl-methoxy-imino-α-(o-tolyloxy)-o-tolyl acetate (BAS 490F) are produced by expressing an antibody capable of binding this fungicide in the plant in a seed- or leaf-specific manner. First, an expression cassette is produced, which contains, among other things, a promoter (preferably CaMV-35S promoter), LeB4 signal peptide, the gene to be expressed and an ER retention signal (so that the accumulated protein is localized in the ER). The expression cassette is then inserted into a recombinant vector (e.g. pBin19), which is transferred to Agrobacterium tumefaciens, which can then be used in a known manner to transform plants, especially crops such as grapes. capable of producing polypeptide or antibody on cultured plants. HE
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19718251A DE19718251A1 (en) | 1997-04-30 | 1997-04-30 | Expression of fungicide-binding polypeptides in plants to produce fungicide tolerance |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| HUP0003594A2 true HUP0003594A2 (en) | 2001-02-28 |
| HUP0003594A3 HUP0003594A3 (en) | 2002-10-28 |
Family
ID=7828212
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HU0003594A HUP0003594A3 (en) | 1997-04-30 | 1998-04-16 | Expression of fungicide-binding polypeptides in plants for producing fungicide tolerance |
Country Status (22)
| Country | Link |
|---|---|
| EP (1) | EP0979295A1 (en) |
| JP (1) | JP2001523101A (en) |
| KR (1) | KR20010020387A (en) |
| CN (1) | CN1254381A (en) |
| AR (1) | AR015626A1 (en) |
| AU (1) | AU737242B2 (en) |
| BG (1) | BG103840A (en) |
| BR (1) | BR9808698A (en) |
| CA (1) | CA2288432A1 (en) |
| DE (1) | DE19718251A1 (en) |
| EA (1) | EA199900889A1 (en) |
| GE (1) | GEP20032959B (en) |
| HU (1) | HUP0003594A3 (en) |
| ID (1) | ID22915A (en) |
| IL (1) | IL132252A0 (en) |
| NO (1) | NO995291L (en) |
| NZ (1) | NZ500181A (en) |
| PL (1) | PL336661A1 (en) |
| SK (1) | SK137299A3 (en) |
| TR (1) | TR199902681T2 (en) |
| WO (1) | WO1998049329A1 (en) |
| ZA (1) | ZA983594B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10033750A1 (en) * | 2000-07-12 | 2002-01-31 | Mpb Cologne Gmbh Molecular Pla | Pathogen resistance in organisms |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1248361B (en) * | 1991-06-28 | 1995-01-05 | Enea | PLASMIDIC VECTORS FOR THE EXPRESSION OF GENES IN PLANTS |
| IL109159A (en) * | 1993-03-29 | 2003-11-23 | Isk Biotech Corp | Immunoassays for tetrachloroiso-phthalonitrile and its metabolites and antibodies for use therein |
-
1997
- 1997-04-30 DE DE19718251A patent/DE19718251A1/en not_active Withdrawn
-
1998
- 1998-04-16 KR KR1019997010009A patent/KR20010020387A/en not_active Application Discontinuation
- 1998-04-16 GE GEAP19985103A patent/GEP20032959B/en unknown
- 1998-04-16 TR TR1999/02681T patent/TR199902681T2/en unknown
- 1998-04-16 WO PCT/EP1998/002242 patent/WO1998049329A1/en not_active Application Discontinuation
- 1998-04-16 IL IL13225298A patent/IL132252A0/en unknown
- 1998-04-16 NZ NZ500181A patent/NZ500181A/en unknown
- 1998-04-16 ID IDW991291A patent/ID22915A/en unknown
- 1998-04-16 BR BR9808698-7A patent/BR9808698A/en not_active IP Right Cessation
- 1998-04-16 HU HU0003594A patent/HUP0003594A3/en unknown
- 1998-04-16 EP EP98920534A patent/EP0979295A1/en not_active Withdrawn
- 1998-04-16 AU AU73356/98A patent/AU737242B2/en not_active Ceased
- 1998-04-16 EA EA199900889A patent/EA199900889A1/en unknown
- 1998-04-16 JP JP54654298A patent/JP2001523101A/en active Pending
- 1998-04-16 SK SK1372-99A patent/SK137299A3/en unknown
- 1998-04-16 CA CA002288432A patent/CA2288432A1/en not_active Abandoned
- 1998-04-16 PL PL98336661A patent/PL336661A1/en unknown
- 1998-04-16 CN CN98804679A patent/CN1254381A/en active Pending
- 1998-04-29 ZA ZA9803594A patent/ZA983594B/en unknown
- 1998-04-30 AR ARP980102047A patent/AR015626A1/en not_active Application Discontinuation
-
1999
- 1999-10-28 BG BG103840A patent/BG103840A/en unknown
- 1999-10-29 NO NO995291A patent/NO995291L/en not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| TR199902681T2 (en) | 2000-07-21 |
| NO995291L (en) | 1999-12-20 |
| ID22915A (en) | 1999-12-16 |
| KR20010020387A (en) | 2001-03-15 |
| JP2001523101A (en) | 2001-11-20 |
| EP0979295A1 (en) | 2000-02-16 |
| IL132252A0 (en) | 2001-03-19 |
| BG103840A (en) | 2000-07-31 |
| PL336661A1 (en) | 2000-07-03 |
| DE19718251A1 (en) | 1998-11-05 |
| CA2288432A1 (en) | 1998-11-05 |
| AU7335698A (en) | 1998-11-24 |
| BR9808698A (en) | 2000-07-11 |
| HUP0003594A3 (en) | 2002-10-28 |
| AU737242B2 (en) | 2001-08-16 |
| ZA983594B (en) | 1999-10-29 |
| GEP20032959B (en) | 2003-04-25 |
| WO1998049329A1 (en) | 1998-11-05 |
| NZ500181A (en) | 2001-02-23 |
| AR015626A1 (en) | 2001-05-16 |
| SK137299A3 (en) | 2000-05-16 |
| CN1254381A (en) | 2000-05-24 |
| NO995291D0 (en) | 1999-10-29 |
| EA199900889A1 (en) | 2001-04-23 |
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