HUE026468T2 - A spiramicinek bioszintézisében részt vevõ polipeptidek, a polipeptideket kódoló nukleotid-szekvenciák és alkalmazásaik - Google Patents
A spiramicinek bioszintézisében részt vevõ polipeptidek, a polipeptideket kódoló nukleotid-szekvenciák és alkalmazásaik Download PDFInfo
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- HUE026468T2 HUE026468T2 HUE07019306A HUE07019306A HUE026468T2 HU E026468 T2 HUE026468 T2 HU E026468T2 HU E07019306 A HUE07019306 A HU E07019306A HU E07019306 A HUE07019306 A HU E07019306A HU E026468 T2 HUE026468 T2 HU E026468T2
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- 0 C*C(C)(C(C(*)(C(C(C*N)[N+])(N)N)N)=*)N Chemical compound C*C(C)(C(C(*)(C(C(C*N)[N+])(N)N)N)=*)N 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
- C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Claims (4)
- Szafeadalpp âgénypestok I* A spksmæmek Moszîiiîëzissber! téSÂksevë pel%>«piide£ kddoto pcdiriiddeobd- äzzsi iüpjjg^ s sôMs#ispyi tüfteppsl&s JstetSvd tPszi & $.piramk'ln«d£ ISTmiltHeBek nôvekedését ês àïssai, lôgjr a polbïukisoîid ssd;Yt'sciéja: |g|?.»,Â31> NO Iï l vagy: Î41 szsmdi szekvsssiäk sgyiks, {!>} oly&o pobatikleodd, -spyelf tegaiÄ |0%, 85%, 90%. 95% v3gy 985b D«ide^jg^aoss%ei mutai a* (a) szerimi polinukleotiddal. I. Az L. igegyponk samrid. pslßiiktetid. aæss! JÂæsepe, fe»gÿ ssppskségbPl :szlppip:0 bakiénambô! van izolaiva. % &spfeajs*a««& bloszfôtszisébeis sfe w6 polipepdd, azzsd Jei&i&ezv«, tegy a tuSisnseiPse 'Mt&MMé îsszl: a ispîtaâsfeïsek bîiasïe^séâuïk advdiedésO as szz&l, bpgy apelipéptid szpkvmkdlia: (a) sdEÇIlO NO Π2 vagy 142 szermn szekvesciilkegyike, Cb) olyaâ pöbpepÄ 3ίίκΝ tegallbh: ÜG%, 85%, 9Ä $5% vagy 98%: amiaosa^azoaoxsagot mutât m %*} szeratii poipeptsddei. 4 A 3, igêîîypoSî szertmi poHpepiid. azzal jelfemerve, à»gy a szekveseùya a SEQ iD NO i 12 vagy 142 szedad szekvesiîidî îariabsazza. 5, A 3. igêîsypost szsdnb pebpepdil azzaî jdJeraeæv®* begy $&êpimt$êm xmnwm£0ti$ saärmazö baktériùïîîbôl vaxi izolalva.
- 6, Exptevs.zK>s vekïor, azzal |*Îé«»8sïy«,; tegy fbgailbb figyg i 3.,. 4>:: vagy 5; igépypaaï: :sz«dad ppipspudet kôdokS nukleinsav-szek^sßCJät iaftalm^K. %Pazilasipï,; axseiy megfelslb sxpresazîdsvekiortiadateaz, as&sly fskatpvdlasglpgy vagy 0M,ia 3., 4. vagy 5. IgpîXypxiri· «.zerind palipupHd expiresxziôjàï. 8. A 7, ïgêîiyposî szeppti g&zdaseîk Jeße«sezv«s feogy gseteiét* gîizdasejtek ès tstoiéiâ gaaaks^tefcMzGÎ vas» ksvaiiasztva. 9. A 8. igénypont szerâsri gazdaseji, azzal jelkßöazve, hsgy E. coïE vovarxejtek, élesztô xajtc-k é.t emlës sejtak kôzüi van kivâiasztva. iÔ. A (gésiypora szerint? gaxiaxejt ansdybß kgaÄb egy. az L vagy 2. îgéiiypômb&H deimiâk polmpktebd «»'vagy kgaMbb egy b;, igéaypsafc szeriad expresszïda vektof leit txxèpitv«. SîÆJMs· a 1-5- %ê?xypOô*ôk Müîïtelÿikô szetidfi polâpepdd eloalbiasâm, azzal feiefttezvg, lïégy az elszaSip^gilbaB fbglaljaa: kôvàtezè ^pesôkdt:; a| apôlipeptidei ké>dôk> Îegalàbb egy xvakkdnvijvfebitkxszidsdt Ebegfciiio vgktorba; li|i àz <t) bkpës szsrmti vekitxral trasazfetmdii: vagy itfsasæM gazdasejt teôÿésziésdi: megîdlaiS tenyésztô tàpkô/eglx;n; c) s kondicionàk lenvdszté ïàpkdzsg vagy .scjt-exirakiimt vxasx<ax;yc'rését; da a poiipspîtd dM^asztâsàt és stegîisztiiâââta e) ïepekbea kapott teßvesp^ ^pkbzegbô! yagy tnasképpen a ^^«stedipppbd!; «} szüfcxèg szermt a termed rekombinäns poilpeptid jtekmzêséî. IS.- As l. vagy 2 . ^x&pommsmÜ pëtimiiâ&^tâ.*SæWmÊs& mÊm&fâsammm· *piœmm& immMsmek javMséra.
- 13. Spirarfiiclfl-îenssU> musclas mikroorgasxizmus, a*?.sl Jelfesôïesv®, bogy taUmnel icgaiâbb egy oly&n kôifolè .szslîvssdâi, amely 1. vagy 2, Igéaypeæî szeribtî szàsm^iM taartatagz, vagy olyaa kèfeM «ïiakvpJÂ, amely 3.. 4. vagy 5. igényporu s^eriap,poljg8p^d«kfeôdei a kérdé.ses kôdolo sxekvstKiâ rùlterntelése xpiramkm-tefcpslê mlkipergasbissuis ik lgésiyp>nî szerluis eypsasaziPs yektorral: vm MsdPvé tèm a. kôdolô: :szskvsscia tâitenîîslésët 34, 3:3:. igsRypoai: szeribli maîsas HîikrotagpHKâïsas, azzal JæHgôîeasvej bogy a: léltertsd egy vagy ii>bb kryplô saekyeßeiäi, asxusly m L igsisypealbaft Ifeîla göinoldeQiid-isxskßPsciäk egyiksi tartalmazza. 15. Λ 13. vagy 34. sgèaypoai szerinu mutans mlayprgasimua, azzal ieltemexve, lisgy a salkfBöfgaxßpsas sxesmsctsdgPe îpfeasé feaMfekm S 6. A 13., 14. vagy 15. tgénypont szermis mutaas roxkrisorgcimxmus. azzal jelteinezve, ®pgy a mikKyàïgpagpas S. wvê^iemm :1¾ E|âfâs s**»ï jdfemezv®, b»gy tnagäPaß fögla|jä a kövdkezö lépéseket: (a) a 13-16. sgénypomok Mrmdys'ke szerinti m ikroorgasiztnus ienyésztdxét a roegfelefô tenyésztô tapkâzegben, h) a kondidonàlt PïRyészîè tàpkôzeg vagy se|feextraktum yfezapypéséî, «> a teæselt spîipsic&ek eîvalaszksàt es îasglisztdâsat a b) lépéi&sîii kapoit teayéâztiëtäpközsgbpi: vagy msskpppan a sejt^xtfakajgpMl
- 18. Strepiomycas an^ß&mS; törzs, gazai jdlemezve* ls©gy sz a ôS€23pÉI?M75|2:|: Idas,, amsly depfiiïâlva lest « Collection f%ôox!afe da Cultures <le Slkroorg&tosmes (CNCM) imézsteél Imksmtgmmsm îaRyéSXëtek tsetsmei gyûjiemény«]. Institut Pasteur. 25. mt du Doctestr Roux 35724 Pans Cedes 1S,; Fraaplaprszäg etase* ·20»3. Oktober fc-àn az 1 -3101 regkztrâdôs: sæcim alatt. If. Eprâs xpirarïdcnpek} îctrodésére, arzal |dtem«æve, bogy tnagciba® iogläfla a kôvetkezé lépésekeî. ^ai a 18. ïgèsypôôt szensii Mkroorganizisvas tenyésztését a megf'cidô tenyésziô tâpkSkdgliep. b) a koïidiclonàit tenvéseto Säpközeg vagy sejtextrakmtn visszaayettäsdE ç) a spiraraiciask elvàlasztâséa: és spagiisztpsat a b) fepéslpp. kapott te-mkztà îâpkôzagbdl vagy iudsk^jpen a SÎgPiâxixakPtixï3>ôL
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR0212489A FR2845394A1 (fr) | 2002-10-08 | 2002-10-08 | Polypeptides impliques dans la biosynthese des spiramycines, sequences nucleotidiques codant ces polypeptides et leurs applications |
| FR0302439A FR2851773A1 (fr) | 2003-02-27 | 2003-02-27 | Polypeptides impliques dans la biosynthese des spiramycines, sequences nucleotidiques codant ces polypeptides et leurs applications |
| US49349003P | 2003-08-07 | 2003-08-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HUE026468T2 true HUE026468T2 (hu) | 2016-05-30 |
Family
ID=36114221
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HUE07019306A HUE026468T2 (hu) | 2002-10-08 | 2003-10-08 | A spiramicinek bioszintézisében részt vevõ polipeptidek, a polipeptideket kódoló nukleotid-szekvenciák és alkalmazásaik |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US7579167B2 (hu) |
| DK (1) | DK1905833T3 (hu) |
| ES (1) | ES2552912T3 (hu) |
| HK (1) | HK1122330A1 (hu) |
| HU (1) | HUE026468T2 (hu) |
| RU (1) | RU2377304C2 (hu) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101914482B (zh) * | 2010-07-23 | 2012-07-04 | 中国医学科学院医药生物技术研究所 | 一株异戊酰螺旋霉素i组分高含量、高产量基因工程菌 |
| US10577594B2 (en) | 2015-07-24 | 2020-03-03 | Novozymes, Inc. | Polypeptides having arabinofuranosidase activity and polynucleotides encoding same |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5068189A (en) | 1988-05-13 | 1991-11-26 | Eli Lilly And Company | Recombinant dna vectors encoding a 4"-o-isovaleryl acylase derived from a carbomycin biosynthetic gene, designated care, for use in streptomyces and other organisms |
| JP2749616B2 (ja) | 1988-05-24 | 1998-05-13 | メルシャン株式会社 | マクロライド抗生物質の４”位アシル化酵素をコードする遺伝子 |
| US5098837A (en) | 1988-06-07 | 1992-03-24 | Eli Lilly And Company | Macrolide biosynthetic genes for use in streptomyces and other organisms |
| US5322937A (en) | 1990-06-01 | 1994-06-21 | Mercian Corporation | Genes encoding a 3-acylation enzyme for macrolide antibiotics |
| US5514544A (en) | 1991-07-26 | 1996-05-07 | Eli Lilly And Company | Activator gene for macrolide biosynthesis |
| CA2197160C (en) | 1996-02-22 | 2007-05-01 | Stanley Gene Burgett | Platenolide synthase gene |
| CA2197524A1 (en) | 1996-02-22 | 1997-08-22 | Bradley Stuart Dehoff | Polyketide synthase genes |
| EP1032679A2 (fr) | 1997-07-25 | 2000-09-06 | Hoechst Marion Roussel | Genes de biosynthese et de transfert des 6-desoxyhexoses chez saccharopolyspora erythraea et chez streptomyces antibioticus et leur utilisation |
| AU2002355157A1 (en) | 2001-07-26 | 2003-02-17 | Ecopia Biosciences Inc. | Genes and proteins for the biosynthesis of rosaramicin |
-
2003
- 2003-10-07 US US10/680,860 patent/US7579167B2/en active Active
- 2003-10-08 DK DK07019306.5T patent/DK1905833T3/en active
- 2003-10-08 ES ES07019306.5T patent/ES2552912T3/es not_active Expired - Lifetime
- 2003-10-08 RU RU2005113874/13A patent/RU2377304C2/ru active
- 2003-10-08 HU HUE07019306A patent/HUE026468T2/hu unknown
-
2008
- 2008-12-12 HK HK08113536.6A patent/HK1122330A1/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| DK1905833T3 (en) | 2015-11-23 |
| US7579167B2 (en) | 2009-08-25 |
| RU2005113874A (ru) | 2006-02-27 |
| HK1122330A1 (en) | 2009-05-15 |
| ES2552912T3 (es) | 2015-12-03 |
| RU2377304C2 (ru) | 2009-12-27 |
| US20050202528A1 (en) | 2005-09-15 |
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