HU202038B - Inbred maize line - Google Patents

Abstract

FIELD OF THE INVENTION The present invention relates to inbred yellow corn line 78010, characterized in that it has a plant height of 140 cm, a heel angle of less than 30 °, a purple color of the ports, a diameter of 42 mm, a hair color of greenish yellow and a weight of 100 eye of 3411 g. Scope of the description: 8 pages, Figure 1 -1

Description

The present invention relates to an inbred maize line.

Zea mays, or corn, is a major crop in many countries around the world, especially in the US. Maize is used for food, feed and industrial purposes.

For the past 60 years, hybrid maize has dominated the trade. As a result of the hybridization, varieties with significantly increased yields, stronger stems and roots, more uniform characteristics and improved resistance to insects and diseases have been developed.

Hybrid maize obtained by simple crossing can be produced by using two homozygous inbred lines as parent. The homozygous character can be achieved by inbreeding within the inbred line, and generally the sixth or seventh inbred generation is considered to be genetically pure.

However, inbreeding for essential genetic identity leads to a reduction in resistance, yield and other plant characteristics. As a result, inbred lines of maize may not be grown for commercial purposes.

Although inbred lines developed through numerous breeding experiments in maize research cannot be commercially grown, they are of particular importance in the production of first generation (F1) hybrids, for example by hybridizing two inbred lines. By crossing two inbred lines, hybrid viability or heterosis occurs, and the hybrid plant has significantly increased yield, strong stem and root, homogeneity, and strong resistance to insects and disease. Self-pollination or cross-pollination of F1 hybrid plants produces second-generation (F2) hybrids. F2 hybrid plants and seeds have less favorable properties than F1 hybrid, thus yielding lower yields and expressing undesirable genetic characteristics. Due to the reduced characteristics, the seeds of the less favorable second generation F2 hybrids F1 are not harvested by growers. On the contrary, breeders expect every year new hybrid seeds obtained by crossing selected inbred parent animals capable of producing first generation (Fi) hybrid seeds.

The production and selection of inbred lines resulting in crossing F1 hybrid hybrids is a specialized and highly specialized task. The function of the breeder is not only to produce and select first-order inbred parent lines, but also to produce hybrid combinations suitable for the production of F1 hybrids and thus for commercialization.

Due to the important role of inbred parent lines in the production of commercial F1 maize hybrids, the development of inbred parent lines in the field of maize cultivation and hybridization has received special emphasis. Thus, it has become necessary to develop new inbred lines that result in first generation hybrid maize with higher yields, greater stability and greater resistance to hybridization. Another requirement is the homogeneity of the first generation hybrids, which facilitates mechanical harvesting and reduces labor requirements.

As a result, there are concerted efforts to develop inbred lines for the production of commercially available first generation maize hybrids.

The invention thus relates to a new inbred maize line DE 78010. BACKGROUND OF THE INVENTION The present invention is DE-78010, an inbred yellow-bellied corn line obtained from the cross of A 634 male and B73 female by hybridization, characterized in that it has a plant height of 140 cm, leaf angle less than 30 ', anthropoid 3411 g.

The invention relates to the pollen of the inbred line DE 78010, which, when fertilized with other maize varieties, produces a high quality first generation (F1) maize hybrid.

The method produces a maize hybrid from an inbred parent line, one of which is the inbred line DE 78010, which produces a first generation (F1) hybrid maize with favorable characteristics such as high yield, good stability, insect and disease resistance. .

Thus, in accordance with the present invention, an inbred corn line DE 78010 is used which has high yield, excellent stalk and excellent greening properties.

In carrying out the method, the hybrid maize is produced by crossing a first inbred corn line with a second inbred corn line, wherein the first inbred corn line or second corn line is an inbred corn line DE 78010, and the other line is fed with a different inbred line.

The invention also encompasses seeds of the inbred line DE 78010.

In the production of a new hybrid maize variety, we use:

(a) planting seeds from the inbred line DE 78010 and seeds from other maize lines in the proximity of pollination;

b) cultivating the plants resulting from planting until flowering;

c) germinating plants from one of the corn lines;

d) cross-pollinating between plants from the inbred line DE 78010 and the other maize line;

e) harvesting the seeds of the plants of the germinated line, wherein the other maize line may be a hybrid maize cross or an inbred maize line, which is preferably not the same as DE 78010.

The inbred line DE 78010 of the invention, when used as a parent line, can be crossed with other maize lines, preferably inbred lines, to form first generation (F1) maize hybrids. Although DE 78010

HU 202038 Β the inbred line is generally used as a female during crossing and can also be used as a male.

The inbred maize line DE 78010 is a yellow horse-bred inbred line and its development is described below:

The A634 hybrid line (male specimen) and the B73 hybrid line (female specimen) available at the University of Minnesota, St. Paul, Minnesota, and Iowa State University, Ames, Iowa, were crossed using standard hybridization techniques. In particular, we crossed the B73 (Iowa Stiff Stalk Synthetic) ker A634 in Dayton, Iowa. We collected SO generation seeds from the harvested tubes and sent them to Homestead, Florida for self-pollination. All harvested tubes are returned to Dayton, Iowa. There we dropped the tubes and collected the S1 generation nuclei. They were planted in a row in Dayton, Iowa. The plants were self-pollinated. Three self-pollinated tubes (S2 generation cores) were harvested, individually milled, and the resulting S2 cores stored separately.

S2 generation seeds from one of the three harvested tubes were planted in five-row blocks in Homestead, Florida and grown to self-pollinate. Five self-pollinated tubes were collected and returned to Dayton, Iowa. The tubes were discarded separately. The resulting generation of seeds were stored separately. Of the five tubes collected, third generation S3 seeds were planted in Dayton, Iowa in a two-row block, and the plants were grown to self-pollination. Four self-pollinated tubes were harvested, chopped separately, and the resulting generation of seeds stored separately.

Of the four tubes collected, fourth generation S4 seeds were planted in row at Homestead, Florida and the plants were self-pollinated. A self-pollinated tube was collected and returned to Dayton, Iowa. S5 generation seeds were chopped and stored there. S5 generation seeds from a single beak were planted in a row in Dayton, Iowa, and the plants were grown to self-pollination. Tubes were not collected. S5 generation seeds were planted in row in Hawaii and the plants were grown to self-pollination. Nine tubes were collected and returned to Dayton, Iowa, where the seeds were collected. The seeds were designated DE 78010. The pure origin of the DE 78010 maize line is maintained by self-pollination and by the accumulation of seeds from selected generations of tubes.

Seeds of the inbred line developed during the described inbreeding were deposited with 40182 at American Type Culture Collection, 12301 Parklawn Drive, Rockville, Maryland 20852.

The invention relates to cells of the plants of the inbred line DE 78010 which can be cultured in a suitable culture, then separated and regenerated to produce plants of the inbred line. Details of the generation process C.E. Green and C.A. Rhodes: Regeneration In Tissue Culture Of Maize Plant; Bread for me

Biological Research - W.F. Sheridan Publisher Plant Molecular Biology Association, Charlottesville, USA, 367-372 (1982).

As mentioned above, the inbred line DE 78010 is yellow horse-tooth maize, which is preferably adapted for cultivation in the northern corn zone of the US, especially in the state of Iowa. (Figure 1)

This inbred line has the following characteristics:

Bee gap

Hatching time to emergence of 50% of corn plants: 81 days (1382 thermal units); seeds with a moisture content of 25% by weight can be harvested 74 days after 50% appearance of corn hair.

Plant

Height: (to wide leaf) 140 cm Pipe height (topmost

tube base) 80 cm Number of shoots: no Number of tubes per stem: average two Type of cytoplasm: normal Letter color: medium (WF9) green Angle: Below 30 ° Sheath fluffiness: medium (WF9) Width: the widest point of the end leaf is 9 cm Number of leaves is ripe plant: 20 pcs crest Number of branches: 6 Branch angle: Below 30 ° ports: clear (WF9) color: purple Shell color: green Tube (unless otherwise noted, data apply to stripped pipes) Length: 16 cm Medium Diameter: 42 mm Magsor: 16, firm, slightly curved Hair color: greenish yellow Extension of the vagina medium (harvest state): (barely covered tube) Leaf sleeve: short (less than 8 cm) peduncle: 9 cm long, intemodium: 5 Apical: average Seed (dry) Size (center of pipe): 10 mm long, 8 mm wide 5 mm thick Shape value (% round): 60-80 Gluten color: white colorless Core color: yellow, white peak Nucleus type: normal starch Weight / 100 seeds (unclassified sample): 34g

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cob

Medium diameter: 26 mm

Length: Strong

Color: red

Disease Resistance Anthracnosis (leaf phase): Resistant

Southern leaf rust (0 varieties): resistant

Eye stain: resistant

The line most closely related to the inbred maize line DE 78010:

Feature: Breed:

Maturity A634Ht

Plant Type A634Ht

Tube Type B73

The unit of heat is calculated using the following formula:

daily well, temp, (30 'C) + daily min, temp. (10 * C) jq ^

The inbred maize line DE 78010 is a yellow inbred inbred line. The closest known maize line is the A634H. The inbred line DE 78010 is statistically significantly different from the known A634Ht inbred line with plant height (140 cm versus 159 cm), leaf angle (23.7 ° versus 44.3 °), anthers color (magenta versus magenta) and hair color (magenta versus magenta). Furthermore, this inbred line shows a significant difference in tube diameter (42 mm versus 38 mm) and weight of 100 cores (34.1 g vs. 29.3 g).

These differences from the commonly known A634Ht inbred line are summarized in the table below:

Isozyme analysis of the inbred lines DE 78010 and A634Ht show that there are at least four different genetic differences,

AcPH-2 instead of 4,

Idh B-6 instead of 4,

MDHB-3.5 instead of 6 and PHI-5 instead of 4.

The isozyme genotype of the inbred line DE 78010 is compared to the known inbred lines B73Ht and A634Ht in Table 2:

Table 2

15 Place Allelomorph presence 78 010 B73Ht A634Ht Checker plants number 6 6 6 ACPH 2 2 4 20 ADH 4 4 4 Cat 9 9 9 INTACT 6 6 6 GOT U 4 4 4 GOT M 4 4 4 25 GOT L 4 4 4 Glu B 7 7 7 IDH A 4 4 4 IDH B 4 4 6 MDH A 6 ^X 6 ^X 6 ^X 30 MDH B 3.5 3.5 6 MDH C 16 16 16 MDH D 12 12 12 MDH E 12 12 12 PGM A 9 9 9 35 PGM B 4 4 4 PHI 4 4 5

x- The allelomorph is assumed to be 6, but zero cannot be excluded.

Table 1

Plant and tube feature 78 010 A634Ht Measurement hypothesis Η ₀ : μι = μ2 If: μι * μ2 1. Plant height 140 cm 159 cm significant (α = 0.1) 2. Angle of leaf 23.7 ° 44.3 ° significant (a-0.1) 3. Color of ports purple greenish yellow 4. Pipe diameter 42 mm 38 mm significant (a = 0, l) 5. Hair color greenish yellow purple 6. Weight of 100 grains 34.1 g 29.3 g significant (a-0.1) 1) N1 + N2

Technique for determination of isozyme gemotype Goodman, M.M. and Stuber, C.W .: Genetic identification of lines and crosses using isoenzyme electrophoresis; Proceedings of the Thirty-Fifth Annual Com and Sorghum Industry Research Coference, 1980.

The inbred line DE 78010 is preferably used as a parent line for the production of hybrid maize, in particular first generation (F1) hybrid maize. When used, this inbred line is used in combination with another inbred line and the two lines 4 are hybridized crosswise with conventional techniques. That is, two inbred lines, one of which is the inbred line DE 78010, are planted in close proximity to each other for pollination. This can be done by placing the parent lines in alternating rows, blocks or any other way. The plants of the two lines are grown until flowering. During the development period, the plants are preferably thinned regularly in the 3-leaf state and regularly treated with fertilizers and / or other agrochemicals for proper development.

HU 202038 Β

During flowering, when the inbred line DE 78010 is used as a male parent, the coat of arms of the other parent is removed from all plants. This removal can be done manually or mechanically.

Female parent individuals can also be chemically sterilized by the use of a cytoplasmic male sterile property. When line DE 78010 is used as a female parent specimen, the coat of arms is removed while pollen develops normally on the other parent specimen.

In both cases, the lines are further cultivated, while the grasses normally self-pollinate under the influence of the wind. As a result of the neutering of the female parent line, pollen from all the male parent lines is suitable for pollination, since all the plants of the inbred lineage and hence the pollen-bearing portions of the female inbred line have been removed. During the hybridization process, the parental individuals are reared separately from corn fields to avoid the undesirable introduction of pollen from foreign varieties. The necessary isolation techniques are well known to those skilled in the art.

Thereafter, both parent lines are further cultivated until maturity, but only tubes from female parent specimens are harvested to harvest the seeds of the new F1 hybrid maize. If necessary, seeds from a male parent can also be harvested for use, but these seeds are not suitable as hybrid seeds.

The resulting new Fi hybrid seeds can be planted next season, when high-yield Fi hybrid maize plants will develop.

The inbred line DE 78010 is suitable for the production of new F1 maize plants which have advantageous properties, for example, the inbred line DE 78010 is used as the female parent line and the inbred line 78060A is used as the male parent line.

The characteristics of the first generation (Fi) DK 524 hybrid were compared with those of the commercially available TI000 variant (Dekalb-Pfizer Genetics). Both variants have similar yields and are particularly suitable for comparison. The results are shown in Table 3.

Table 3

Hybrid DK 524 T1000 Fruit (bush Seed moisture 137.4 121.2 * Contents (t%) Plant height 23.0 23.3 * (Inch) (91.2) (78.9) Pipe height 228 cm 197.25 cm * (Inch) (44.5) (34.6) 113.25 cm 86.50 cm * green Ground The stem is not 96.3 85.9 * lies Crop moisture 99.5 100.1 * contents 1 inch-2.54 cm 1 bus = 36.35 1 114.0 98.9 *

* -1% statistically significant

It can be seen from the above that the inbred line DE 78010 provides an advantageous strain of growth when used as a female parent line for the production of first generation hybrids with special and advantageous properties. It is similarly advantageous for use as a male parent.

Although the invention has been described in detail and in all respects, it will be apparent to those skilled in the art that various changes and modifications are possible without departing from the spirit and scope of the invention.

Claims (1)

DE-78010 is an inbred yellow horse-toothed corn line, obtained from a cross between A 634 male and B73 female, by hybridization characterized by a plant height of 140 cm, leaf angle less than 30 °, anthropoid purple, tubular green 42 mm, hair color.