HU201239B - Quail egg based hair dyeing and hair regenerating cosmetic - Google Patents

Abstract

The composition of the present invention comprises 80-95% by weight of emulsion and 5-20% by weight of propellant, wherein the emulsion comprises 5-25% by weight of smoked eggs, 10-33% by weight of washing agent / materials, preferably fatty acid amidoalkyl betaine, fatty acid glycerol-poly (ethylene glycol) ether, hydroxyethylalkylmmonium phosphate and / or vinylpyrrolidone copolymer, 55-75% by weight of water, 0-5% by weight of cosmetic additives / materials, preferably organic carboxylic acid and / or either a fragrance, a coloring agent and a preservative are present, and the emulsion as a dye and preservative is from 0.1 to 5.0% by weight of 7- (trimethylammonium) -1 - [(3-nitro-4-aminophenone) of formula (I) - azo-beta-naphthol chloride and / or 7- (trimethylammonium) -1 - ((2-nitro-4-aminophenyl) azo) beta-naphthol chloride and / or 7- (trimethylammonium) -1 - [(4-aminophenyl) azo] -beta-naphthol chloride and / or 7- (trimethylammonium) of formula (IV) - 2-methoxyphenyl) azo] beta-naphthol chloride and / or (V) 2-br6-imino-5-hydroxy-6 [3- (trimethylammonium) phenylamino] -8-aminonaphthokinone / 1,4-chloride and / or 2,6-dibromo-4-imino- (VI) 5-Hydroxy-8- [3- (trimethylammonium) phenylamino] naphthokinone / 1,4-chloride and / or 1-phenyl-2-hydron-3-yl-trimethyl- ammonium-phenyl-azo-4-methyl-pyrazole chloride at pH 3-5. (I) G0 (111) References: Page 7, Figure 2 EN 201 239 A -1-

Description

The present invention relates to a quail egg coloring and hair care composition, more particularly to a foam composition,

80-95% by weight of emulsion and 5-20% by weight of propellant, wherein the emulsion contains 5-25% by weight of quail eggs, 10-33% by weight of detergent / material, 55-75% by weight of water, 0-5% by weight cosmetic additive (s), and a dye and a preservative.

Cosmetic preparations are known which can simultaneously color and wash the hair. Thus, U.S. Pat. A preparation known from European Patent Specification No. 2,983,125 contains detergent active substances and colorants [Chem. Abstr., 26, 213011η (1982)]. Shampoo containing hen egg yolk is described in 2.134.070. s. French Patent No. 5,198,198, Chem. 15 Abstr., 2S, 151573a (1973)]. A 79-08607. s. Japanese Patent Publication [Chem. Abstr., 21,

44400p (1979)] is known for a liquid shampoo containing dyes, detergents and dried egg white. 20

A 185,849. Pat. Hungarian patent application describes preserved cosmetic foam preparations based on quail eggs, including shampoo and hair wrap. Considering that quail eggs have higher lecithin, vitamin A and essential 25 amino acids than chicken eggs [Chem. Abstr., 22, 77108e (1973); 22.37669d (1982)], its skin nourishing effect is very valuable. Since the coloring of the hair dye requires prolonged contact with the hair, typically 10 to 20 minutes, it seems desirable to use a hair coloring composition 30 which has a beneficial effect on the hair and scalp during the hair dyeing period.

A 185,849. Pat. There is no reference in Hungarian Patent Specification to a hair coloring preparation, although one or more coloring agents are listed in the list of possible additives without giving a specific example.

The above anterior quail egg-based formulations are preserved with a special stabilizer system 40 containing 0.1 to 1% by weight of one or more aseptic materials, such as boric acid, sorbic acid, benzoic acid, salicylic acid, phydroxybenzoic acid, p-hydroxybenzoic acid, ester and / or beta-phenylalkanol and 1-3% 45 by weight of 2,4-diamino5- (3 ', 4', 5'-trimethoxybenzyl) pyrimidine (1: 5- (5: 1) by weight) The known stabilizer system achieves a shelf life of 2 years or more than 2 years, while the individual components of the stabilizer system alone provide stability for only a few months.

It is known that some of the dyes used in the textile industry are also antibacterial [dr. Péter Ferenc, Dye Handbook, Technical Publisher, Budapest, 1968, 252-253. side]. This is the highest at pH 9 and only about one-hundredth of the activity at pH 9. [Handbook of the Cosmetic Industry, Technical Publication, Budapest, 1962,192. Some of these dyes may also be used in principle for hair dyeing, but the antimicrobial activity is not so strong as to provide permanent stabilization of the quail egg-based formulation for hair dyeing at pH 3-5. Table 1 shows the bacterial and fungal activity of some known dyes and, for comparison, some known, highly effective antimicrobial agents. The in vitro assay was performed as follows.

Minimum inhibitory concentrations of the dyes against the following strains of microorganisms were determined by an effective method in phenol red-glucose broth (manufactured by DIFCO) and in fungal strains in Sabouraud broth:

A - Staphylococcus aureus HNCMB 112003

B - Pseudomonas aeruginosa HNCMB 170021

C - Echerichia coli HNCMB 30016

D - Candida albicans ATCC 752 "

E - Aspergjllus niger ATCC1004 ** ^x National Collection of Medical Bacteria, Genetic List, 1978.

American Type Culture Collection, Catalog of Strain, No. 1.15. edition, 1982.

Culture at bacterial strains at 37 ° C,

The pH was 7-7.2, and the fungal strains at 26 * C had a pH of 63-6.7. The

Table 1 shows the minimum inhibitory concentration (MIC) for microbidd activity, that is, which permanently inhibited the growth of microorganisms and thus the inoculated media remained sterile.

Table I

Test substance

MIC value, pg / ml

For B C D E microorganisms

dyes:

7- (Trimethylammonium) -1 - [(3-nitro-4-aminophenyl) azo] -beta-naphthol chloride, which is 7- (trimethylammonium) -1 - [(2-nitro-4) -aminophenyl) -azo] -beta-naphthol also contains 6.4 54 102 12 240

7- (Trimethylammonium) -1- [4- (aminophenyl) azo] -beta-naphthol chloride 8.4 56 100 · 106 600

-2HU 201239 A * 4

Continuation of Table I

Test substance THE B mil MIC value, µg / ml C croorganisms D TEN E 7- (trimethylammonium) -l - [(2-methoxy- phenyl) azo] -beta-naphthol sulfonylchlaride 34 136 200 112 1000 2-Bromo-4-imino-5-hydro-6- [3- (trimethylammonium) phenylamino] -8-aminonaphthoquinone (1,4) chloride and 2,6-dibromo-4- Mixture of imino-5-hydroxy-8- (3- (trimethylammonium) phenylamino) -naphthalnone (1,4) chloride 28 92 98 112 200 l-Phenyl-2-hidroá-3- [3- (trimethyl- ammonium) -phenylazo] -4-methyl-pyrazole -chloride 80 460 560 360 1400 Pharmaceutical Ingredients: Chloroquinaldol [5,7-dichloro-2-methyl-8-hydroxyquinoline] 0.5 200 5 1 2 clotrimazole (1- (2-chlorophenyl) diphenylmethyl-1H-imidazole) 1 200 200 0.5 1 Quinoline-8-hidrori sulfate 5 20 25 15 25 polimixinB 5 5 5 25 70 A mixture of 1 part by weight of trimethoprim _f and 5 parts by weight of sulfamethazole 2 2 1 15 50

6,9,18-tris (2-aminoethyl) -15-benzyl-21- (2,8bis (2-aminoethyl) -5- (1-hydroxyethyl) -15-diethyl-4 , 7, 35 10-trioxo-3,6,9-triaza-heptadecanamido] -3- (1-hydroxyethyl) -12-isobutyl-1,4,7,10,13,16,19- heptaazaciklotrikozán-2 ^ 8 ₁ ll, 14,17,20-hcptaon

2,4-Diamino-5- (3 ', 4', 5'-trimethoxybenzyl) - pyridine 40

3- (4'-Aminophenylsulfonylamino) -5-methylisoxazole

Table 1 clearly demonstrates that the drug substances tested inhibit the growth of the bacterial and fungal strains at significantly lower concentrations than the dyes, but it is known from a mixture of trimethoprim and sulfamethoxazole that it has stabilized the quail egg preparation for only 3 months [v. She. No. 185,849. Hungarian Patent Specification]. Therefore, one of ordinary skill in the art would expect the dyes listed in Table 1 to have practically no stabilizing effect on quail egg formulations.

Surprisingly, it has been found that the dyes of general formula (I), (Q), (III), (IV), (V), (VI) and / or (VE), either individually or in combination, or more collectively effectively stabilize quail egg-based cosmetic formulations, which can be stored for at least 2 years when stored at 25 ° C, and color when used to provide hair coloring.

This observation is all the more surprising since the actual antimicrobial activity of the dyes in the formulation at the pH of 3-5 to achieve the coloring effect is, according to the literature, only 65% of that of the higher H-value.

Thus, in the foam composition of the present invention, the emulsion used is from 0.1 to 5.0% by weight of 7- (trimethylammonium) -1 - [(3-nitro-4-aminophenyl) -azo] -beta-naphthol as a coloring agent and preservative. chloride and / or 7- (trimethylammonium) -1 - [(2-nitro-4-aminophenyl) azo] -beta-naphthol chloride of formula (Π) and / or 7- (trimethylammonium) of formula (ΙΠ) ammonium) -1 - [(4-aminophenyl) -azo] -beta-naphthol chloride and / or 7- (trimethylammonium) -1 - ((2-methoxyphenyl) -azo] -beta-naphthol chloride and / or 2-bromoimino-5-hydroxy-6- [3- (trimethylammonium) phenylamino] -8-aminonaphthmonone (1,4) -chloride and / or 2,6-dibromo-4-imino-5-hydro-8- [3- (trimethylammonium) phenylamino] -naphthoquinone (VI), 1,4-chloride and / or (VII) 1-Phenyl 2-hydroxy-3- (3- (trimethylammonium) phenyl] azo] -4-methylpyrazole chloride of formula IIIa at a pH of 3-5.

The foam formulation of the present invention consists of an emulsion and a propellant present before use. The emulsion is foamed by the propellant when pressed out of the aerosol can. The pre-formulation contains 80-95% by weight of an aqueous emulsion and 5-20% by weight of a propellant.

Ax emulsion contains from 5% to 25% by weight of quail eggs, 10% to 33% of one or more detergents, 5575% of water, 0% to 5% of one or more cosmetic additives, and 0.1% to 5% of one or more of the above colorants.

Eggs of quail eggs (Coturnixcoturnix), that is, available after breaking eggs

-3EN 201239 We mean material which can be whole eggs (natural egg juice), egg yolks, whites, whole eggs enriched with yolks or proteins, all in wet or lyophilized form.

The detergent may be anionic, cationic or nonionic surfactant commonly used in shampoos for this purpose, of which one or more may be present. Betaine derivatives substituted with a fatty acid aminoalkyl group, polyethylene glycol ethers of fatty acid glyceride, hydroxyethylalkylammonium phosphates, quaternized vinylimidazole and vinylpyrrolidone have been found to be preferred for this purpose.

The water present in the composition of the invention is preferably distilled water or deionized water.

The cosmetic additive is one or more additives customary in cosmetic compositions, for example one or more organic acids such as nitric or tartaric acid, one or more fragrances, one or more bioactive substances such as vitamins, solubilized collagen, herbal extracts such as chamomile extract and the like. may.

The propellant for foaming the emulsion may be a gas or gas mixture conventionally used in aerosol formulations, for example fluorinated and / or chlorinated hydrocarbon (s) such as dichlorodifluoromethane, dichlorotetrafluoroctane and mixtures thereof, propane-butane mixture, and the like.

The composition of the present invention is prepared by mixing its ingredients to form an emulsion, filling into an aerosol can, sealing the can and filling the propellant.

In preparing the emulsion, it is convenient to dissolve the dye in water, optionally with heating and stirring, and then to add to the solution the detergent (s) which are added in anhydrous or aqueous solution, and finally the cosmetic additive (s) and quail eggs. any remaining portion and homogenize the emulsion.

In use, the bottle valve is depressed; the propellant is then depressed and foamed at the same time. Apply the amount of foam needed for dyeing to wet hair and leave it on for 10-20 minutes, then wet the hair to form a rich foam, rinse with plenty of water and dry.

The composition of the invention may be stored for at least 2 years under normal storage conditions. One or more colorants present in the composition also provide stability to the composition. When coloring the hair during the required treatment time, the composition of the egg-grass component of the composition has a beneficial effect on the hair and scalp as a wrap.

The invention is illustrated in detail by the following examples.

Example 1

Blonde coloring hair care foam

Composition of the foam convertible emulsion:

parts

Tego-Betaine L 7 (fatty acid amidoalkyl betaine

35% aqueous solution 30

Rewoderm LI67 (Coconut fatty acid glyceride4

-polietilénglikoléter 75% aqueous solution) 10 Eggs (natural egg juice) 10 Citric acid 0.25 5 IUatanyag 7- (Trimethylammonium) -1 - [(3-nitro-4-aminophenyl) azo] -beta-naphthol chloride, which is 7- (trimethylammonium) 0.10 10 contains ammonium) -1 - [(2-nitro-4-aminophenyl) -azo] -beta-naphthol chloride isomer (Color Index number: 12251) 035 15 1-Phenyl-2-hydroxy-3- [3- (trimethylammonium) phenyl azo] -4-methylpyrazole chloride (Color Index number: 12719) 035 20 Distilled water 48.95 100.00

The foam formulation consists of 90 parts by weight of an emulsion having a pH of 3.6 and 10 parts by weight of propellant gas.

The dyes are dissolved with heating and stirring in about half of the indicated amount of water, citric acid, detergents, leach, and finally the natural foam egg and the rest of the water are added to the solution. The emulsion is homogenized, filled into an aerosol can, sealed and charged with propellant. The formulation retains its stability for 2 years and can be used during this time for hair dyeing and washing with blond hair.

Example 2

Brown Foam Conditioner The foam-emulsion is composed of:

40 parts Chicken eggs (natural egg juice) Tego-Betaine L 7 (fatty acid amidoalkyl betaine) 20 35% aqueous solution 30 45 Rewoderm LI 67 (Coconut fatty acid glyceride -polietilénglikoléter 75% aqueous solution) 10 Citric acid 035 50 IUatanyag 2-Bromo-4-imino-5-hydroxy-6- [3- (trimethylammonium) -phenylamino] -8-amino-nitroquinone (1,4) chloride and 0.10 55 Mixture of 2,6-dibromo-4-imino-5-hydroxy-8- [3- (trimethylammonium) phenylamino] naphthoquinone (1,4) chloride (Color Index 60 number: 56059) 7- (Trimethylammonium) -1- [4- (aminophenyl) azo] beta-naphthol hydrochloride (Color 030 Index number: 12550) 0.60 65 7- (trimethylammonium) -l-

-4EN 201239 A

- [(3-nitro-4-amino-phenyl) -azo] -beta-naphthalene chloride which is 7- (trimethylammonium) -1 - [(2-nitro-4-aminophenyl) -azo] - also contains beta-naphthol chloride isomer (Color Index No: 12251) 0.60

Distilled water 38.15

100.00

The foam formulation comprises 80 parts by weight of the above emulsion having a pH of 4.1 and 20 parts by weight of propellant.

The dyes are dissolved in a portion of the volume of water with heating and stirring, citric acid, detergents, and separately dissolved egg powder are added to the rest of the water. The homogenized emulsion is filled into an aerosol can, the can is sealed and the propellant is filled.

The product retains its stability for 2 years and can be used during this time for brown tinted hair care and hair washing.

Example 3

Mahogany coloring hair care foam

The composition: Quail eggs (Uofilized protein-enriched egg juice) Tego-Betaine L 7 (fatty acid amidoalkyl betaine) parts 10.0 35% aqueous solution Rewoderm LI67 (Coconut fatty acid gEcerid- -polietilénglikoléter 30 75% aqueous solution) 10 Citric acid 0.25 IUatanyag 7- (Trimethylammonium) -1- [4- (aminophenyl) azo] beta-naphthol chloride (Color 0.10 Index number: 12550) 1.50 Distilled water 48.15

100.00

The foam formulation comprises 95 parts by weight of the emulsion of the above composition, pH 3.9, and 5 parts by weight of propellant.

The formulation was prepared as described in Example 2. It retains its stability for 2 years and can be used within this period for washing red-colored hair.

4.péla

Cremating hair care foam preparation

Composition of the foam convertible emulsion:

parts

Quail eggs (lyophilized, protein - enriched egg juice)

Dehyton K (coconut amidopropyl betaine

32% aqueous solution) 42.0

Dehyquart SP (Hydroxy

ethyl alkyl ammonium 50% aqueous solution of phosphate) Luviquat FC 905 (methyl vinyl imidazolium chloride and vinyl) 2.0 -pirroEdon copolymer) 1.5 Tartaric acid 0.25 IUatanyag 2-Bromo-4-imino-5-hydroxy- -6- [3- (trimethylammonium) - phenylamino] -8-amino- -naphthoquinone (1,4) chloride and 2,6-dibromo-4-amino-5- hydroxy-8- [3- (trimetU- ammonium) phenylamino] - naphthoquinone (l, 4) chloride blend (Color Index 0.1 number: 56059) 7- (Trimethylammonium) -1 - [(2-metho-phenyl) -azo] -beta-naphthol chloride (Color Index) 0.12 number: 12245) 7- (Trimethylammonium) -1 - [(3-nitro-4-aminophenyl) azo] -beta-naphthol chloride, which is 7- (trimethylammonium) -1 - [(2-nitro-4) also contains the isomer-phenyl-azo] -beta-naphthol chloride (Color Index) 0,025 number: 12251) 0,005 Distilled water 44,00

100.00

The foam formulation comprises 90 parts by weight of an emulsion of pH 4.8 and 10 parts by weight of propellant.

The composition was prepared as in Example 1. Shelf life is up to 2 years and during this time it can be used for hair care combined with cremation coloring and for hair washing.

Example 5

Black Color Hair Treatment Foam Emulsion Contains:

Quail eggs (natural egg juice) parts 10.0 Tego-Betaine L 7 (35% aqueous solution of fatty acid amidoalkyl betaine) 25.0 Rewoderm LI 67 (Coconut fatty acid glyceride -poEetilénglikoléter 75% aqueous solution) 10.0 Citric acid 0.25 IUatanyag 0.10 2-Bromo-4-imino-5-hydroxy- -6- [3- (trimethyl ammonium) - phenylJamino-amino] -8-amino- -naphthoquinone (1,4) chloride and 2,6-dibromo-4-amino-5- hydroxy-8- [3- (trimetU- ammonium) phenylamino] - naphthoquinone (l, 4) chloride blend (Color Index number: 56059) 0.10 7- (trimethylammonium) -l- 5

-5HU 201239 A

- [(3-nitro-4-aminophenyl) -azo] -beta-naphthol id which is 7- (trimethylammonium) -1 - [(2-nitro-4-aminophenyl) -azo] - also contains beta-naphthol chloride isomer (Color Index No: 12251) 0.27

7- (Trimethylammonium) -1 ((2-methoxyphenyl) azo] -beta-naphthol chloride (Color Index No: 12245) 0.13

Distilled water 53.15

100.00

The foam formulation comprises 90 parts by weight of an emulsion of pH 4.4 and 10 parts by weight of propellant.

The composition was prepared as described in Example 1. Shelf life is up to 2 years and during this time it can be used for black hair tanning and hair washing.

Example 6

Dark Black Color Hair Treatment Foam Emulsion Contains:

Eggs (lyophilized) parts whole eggs) Tego-Betaine L 7 (fatty acid amidoalkyl betaine 5.0 35% aqueous solution Rewoderm LI 67 (Coconut fatty acid glyceride -polietilénglikoléter 50.0 75% aqueous solution) 20.0 Citric acid 0.4 lllatanyag 2-Bromo-4-imino-5-hydroxy- -6- [3- (trimethylammonium) - phenylamino] -8-amino- -naphthoquinone (1,4) chloride and 2,6-dibromo-4-amino-5- hydroxy-8- [3- (trimethyl- ammonium) -phenyl -propionic naphthoquinone (l, 4) chloride blend (Color Index 0.1 number: 56059) 7- (Trimethylammonium) -1 - [(3-nitro-4-aminophenyl) azo] -beta-naphthol chloride which is 7- (trimethylammonium) -1 - [(2-nitro-4) -aminophenyl) -azo] -beta-naphthol chloride also contains isomer (Color Index 3.6 number: 12251) 7- (Trimethylammonium) -1 - [(2-metha-phenyl) -azo] -beta-naphthol chloride (Color Index) 0.85 number: 12245) 0.55 Distilled water 19.5 100.00 The foam formulation comprises 85 parts by weight pH 3.2

emulsion and 15 parts by weight of propellant.

The dye is dissolved in the specified amount of water with heating and stirring. Citric acid, detergents, lyophilized quail egg and perfume are added to the solution. The emulsion is homogenized, filled into an aerosol can, sealed and charged with propellant.

The product has a shelf life of up to 2 veins and can be used during this time for dark-hair tinted hair care and hair washing.

Example 7

Blonde Hair Coloring Foam Formulation

Tego-Betaine L 7 (fatty acid amidoalkyl betaine parts 35% aqueous solution Rewoderm LI 67 (Coconut fatty acid glyceride -polietilénglikoléter 20 75% aqueous solution) 4 Quail eggs (natural egg juice) 25 Citric acid 0.25 lllatanyag 0.10 Collagen (solubilized) 7- (Trimethylammonium) -1 - [(3-nitro-4-aminophenyl) azo] -beta-naphthol chloride, which is 7- (trimethylammonium) -1 - [(2) also contains the isomer of nitro-4-aminophenyl) -azo] -beta-naphthol chloride (Color Index 4.5 number: 12251) 1-Phenyl-2-hydroxy-3- [3- (trimethylammonium) phenyl azo] -4-methylpyrazole chloride (Color Index number: 0.35 12719) 0.35 Distilled water 45.45

100.00

The foam formulation comprises 90 parts by weight of the above emulsion, pH 3.8, and 10 parts by weight of propellant.

The preparation is carried out as in Example 1. Collagen is added before the natural quail egg juice.

The product maintains its stability for up to 2 vessels.

Stabililásvizsgólat

The shelf-life of the composition of Example 1 was compared with that of similar compositions containing 4% by weight of one of the active ingredients listed in Table 1 instead of dyes. (Because the dye content was only 0.7% by weight, the water content was reduced by 33% by weight with the drug.) Pat. The test was performed as described in Hungarian Patent Application: at weekly storage at 25 ° C, samples were taken weekly and the samples were subjected to organoleptic examination (consistency and odor of the expelled foam). Loose, fluffy foam, or mild or stronger,

-6EN 201239 The odor reminiscent of the crab egg indicated a lack of stability.

Table 2 shows the preservative tested and the time to weeks until the formulation was stable. Each sample was stored until degradation of the formulation or for 2 years for the formulation of Example 1.

spreadsheet

Preservative Stability time is seven chloroquinaldol 4 clotrimazole 6 Quinoline-8-hidrori -sulfate 4 polimixinB 9 trimethoprim and sulfometaxazole 1: 5 by weight mixture 12 Mixture of dyes of formulas I, II and VII (Preparation of Example 1) 104

Table 2 shows that antimicrobial agents that are more potent in vitro than the dyes used provide only stability to the formulation for 4 to 12 weeks, whereas relatively ineffective dyes in vitro provide long term shelf life.

Claims (1)

Quail egg hair coloring and hair care composition, containing 80-95% by weight of emulsion and 5-20% by weight of propellant, wherein the emulsion contains 5 to 25% by weight quail eggs, 10 to 33% by weight of detergent active substance (s), preferably fatty acid amidoalkyl betaine, fatty acid glyceride poly (ethylene glycol) ether, hydroxyethyl alkyl ammonium phosphate and / or or vinylpyrrolidone copolymer, 55-75% by weight water, 0-5% by weight cosmetic additive (s), preferably organic carboxylic acid and / or fragrance, and a dye and a preservative, characterized in that the emulsion is 0.1 -5.0% by weight of 7- (trimethylammonium) -1 - [(3-nitro-4-aminophenyl) azole-beta-naphthol chloride of formula (I) and / or (trimethylammonium) -1 - [(2-nitro-4-aminophenyl) -azo] -beta-naphthol chloride and / or 7- (trimethylammonium) -1 - [(4) -aminophenyl) -azo] -beta-naphthol chloride and / or 7- (trimethylammonium) -1 - [(2-methoxyphenyl) -azo] -beta-naphthol chloride and / or 2-Bromoimino-5-hydro-6- [3- (trimethylammonium) phenylamino] -8-aminonaphthoquinone (V): 1,4-Chloride and / or 2,3-dibromo-4-imino-5-hydro-8- [3- (trimethylammonium) phenylamino] naphthoquinone (VI), 1,4-chloride and / or 1-phenyl-2 (VII) -hydroxy-3- (3- (trimethylammonium) phenyl-azo] -4-methylpyrazole-uride at a pH of 3-5.