HK1062014A1 - Labelling reagents, method for synthesis of said reagents and methods for detecting biological molecules - Google Patents
Labelling reagents, method for synthesis of said reagents and methods for detecting biological moleculesInfo
- Publication number
- HK1062014A1 HK1062014A1 HK04105062.8A HK04105062A HK1062014A1 HK 1062014 A1 HK1062014 A1 HK 1062014A1 HK 04105062 A HK04105062 A HK 04105062A HK 1062014 A1 HK1062014 A1 HK 1062014A1
- Authority
- HK
- Hong Kong
- Prior art keywords
- reagents
- labelling
- synthesis
- biological molecules
- alkyl
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
- C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
- C07D209/04—Indoles; Hydrogenated indoles
- C07D209/08—Indoles; Hydrogenated indoles with only hydrogen atoms or radicals containing only hydrogen and carbon atoms, directly attached to carbon atoms of the hetero ring
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C245/00—Compounds containing chains of at least two nitrogen atoms with at least one nitrogen-to-nitrogen multiple bond
- C07C245/12—Diazo compounds, i.e. compounds having the free valencies of >N2 groups attached to the same carbon atom
- C07C245/14—Diazo compounds, i.e. compounds having the free valencies of >N2 groups attached to the same carbon atom having diazo groups bound to acyclic carbon atoms of a carbon skeleton
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C245/00—Compounds containing chains of at least two nitrogen atoms with at least one nitrogen-to-nitrogen multiple bond
- C07C245/12—Diazo compounds, i.e. compounds having the free valencies of >N2 groups attached to the same carbon atom
- C07C245/14—Diazo compounds, i.e. compounds having the free valencies of >N2 groups attached to the same carbon atom having diazo groups bound to acyclic carbon atoms of a carbon skeleton
- C07C245/18—Diazo compounds, i.e. compounds having the free valencies of >N2 groups attached to the same carbon atom having diazo groups bound to acyclic carbon atoms of a carbon skeleton the carbon skeleton being further substituted by carboxyl groups
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/78—Ring systems having three or more relevant rings
- C07D311/80—Dibenzopyrans; Hydrogenated dibenzopyrans
- C07D311/82—Xanthenes
- C07D311/90—Xanthenes with hydrocarbon radicals, substituted by amino radicals, directly attached in position 9
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D495/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms
- C07D495/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms in which the condensed system contains two hetero rings
- C07D495/04—Ortho-condensed systems
Abstract
The invention concerns a temperature-stable labelling reagent of formula (I), wherein: R<1> represents H or an alkyl, aryl or substituted aryl group; R<2> represents a detectable marker or at least two detectable markers linked together by at least a multimeric structure; L is a linking arm comprising a linear catemer concatenation of at least two covalent bonds; and n is an integer equal to 0 or 1; R<3> and R<4> represent, independently of each other: H, NO2, Cl, Br, F, I, R<2>-(L)n-Y-X-, OR, SR, NR2, R, NHCOR, CONHR, COOR with R = alkyl or aryl, A is a linking arm comprising at least a double bond enabling the conjugation of the diazo function with the aromatic cycle; and u is a whole number between 0 and 2, preferably between 0 and 1; and -Y-X- represents -CONH-, -NHCO-, -CH2O-, -CH2S-. The invention also concerns a method for the synthesis of said markers and uses for labelling biological molecules, in particular nucleic acids, with a labelling reagent bearing the diazomethyl function. The invention is particularly applicable in the field of diagnosis.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR0106040A FR2824323B1 (en) | 2001-05-04 | 2001-05-04 | MARKING REAGENT AND METHOD FOR DETECTING BIOLOGICAL MOLECULES |
PCT/FR2002/001543 WO2002090319A1 (en) | 2001-05-04 | 2002-05-03 | Labelling reagents, method for synthesis of said reagents and methods for detecting biological molecules |
Publications (1)
Publication Number | Publication Date |
---|---|
HK1062014A1 true HK1062014A1 (en) | 2004-10-15 |
Family
ID=8863021
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
HK04105062.8A HK1062014A1 (en) | 2001-05-04 | 2004-07-12 | Labelling reagents, method for synthesis of said reagents and methods for detecting biological molecules |
Country Status (11)
Country | Link |
---|---|
EP (1) | EP1383732B1 (en) |
JP (2) | JP4494717B2 (en) |
CN (1) | CN100509761C (en) |
AT (1) | ATE457973T1 (en) |
AU (1) | AU2002314227B2 (en) |
CA (1) | CA2444918C (en) |
DE (1) | DE60235354D1 (en) |
ES (1) | ES2339337T3 (en) |
FR (1) | FR2824323B1 (en) |
HK (1) | HK1062014A1 (en) |
WO (1) | WO2002090319A1 (en) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2824335A1 (en) * | 2001-05-04 | 2002-11-08 | Bio Merieux | DNA MARKING AND FRAGMENTATION PROCESS |
FR2824323B1 (en) * | 2001-05-04 | 2008-04-25 | Bio Merieux | MARKING REAGENT AND METHOD FOR DETECTING BIOLOGICAL MOLECULES |
CA2551495A1 (en) * | 2004-01-07 | 2005-07-28 | Ambit Biosciences Corporation | Conjugated small molecules |
FR2868071B1 (en) | 2004-03-26 | 2006-06-09 | Biomerieux Sa | MARKING REAGENTS, METHODS FOR SYNTHESIZING SUCH REAGENTS AND METHODS FOR DETECTING BIOLOGICAL MOLECULES |
FR2881437B1 (en) | 2005-01-31 | 2010-11-19 | Biomerieux Sa | METHOD FOR THE DIAGNOSIS / PROGNOSIS OF A SEPTIC SYNDROME |
FR2886735B1 (en) * | 2005-06-01 | 2015-09-11 | Biomerieux Sa | PROCESS FOR MARKING OR PROCESSING A BIOLOGICAL SAMPLE CONTAINING BIOLOGICAL MOLECULES OF INTEREST, IN PARTICULAR NUCLEIC ACIDS |
GB0616724D0 (en) | 2006-08-23 | 2006-10-04 | Isis Innovation | Surface adhesion using arylcarbene reactive intermediates |
FR2917090B1 (en) * | 2007-06-11 | 2012-06-15 | Biomerieux Sa | MARKING REAGENTS HAVING DIAZO AND NITRO FUNCTIONS, METHODS FOR SYNTHESIZING SUCH REAGENTS AND METHODS FOR DETECTING BIOLOGICAL MOLECULES |
FR2934595B1 (en) * | 2008-07-29 | 2013-04-05 | Biomerieux Sa | MARKING REAGENTS HAVING A PYRIDINE CORE HAVING DIAZOMETHYL FUNCTION, METHODS FOR SYNTHESIZING SUCH REAGENTS AND METHODS FOR DETECTING BIOLOGICAL MOLECULES |
EP2403825B1 (en) | 2009-03-02 | 2019-09-11 | Oxford Advanced Surfaces Ltd | Chemical agents capable of forming covalent 3-d networks |
EP2576815B1 (en) | 2010-06-04 | 2018-02-14 | Biomérieux | Method for the prognosis of colorectal cancer |
US9110079B2 (en) | 2010-09-29 | 2015-08-18 | Biomerieux | Method and kit for establishing an in vitro prognosis on a patient exhibiting SIRS |
FR2968302B1 (en) | 2010-12-06 | 2012-11-30 | Biomerieux Sa | METHODS OF FUNCTIONALIZATION AND REAGENTS USED IN SUCH METHODS USING ISATOIC ANHYDRIDE OR ONE OF ITS DERIVATIVES, BIOLOGIC MOLECULES SO TREATED AND KITS |
WO2012129758A1 (en) | 2011-03-25 | 2012-10-04 | Biomerieux | Method and kit for determining in vitro probability for individual to suffer from colorectal cancer |
FR2994184B1 (en) | 2012-08-02 | 2017-12-08 | Biomerieux Sa | METHODS OF FUNCTIONALIZATION AND REAGENTS USED IN SUCH METHODS USING AN AZA-ISATOIC ANHYDRIDE OR ONE OF ITS DERIVATIVES, BIOLOGIC MOLECULES SO TREATED AND KITS |
KR101953819B1 (en) * | 2017-12-22 | 2019-03-06 | (주)바이오액츠 | Novel cyanine compound for labeling biomolecule and preparation method thereof |
CN113636931B (en) * | 2021-08-05 | 2024-02-13 | 康龙化成(宁波)科技发展有限公司 | Gene coding compound library initial fragment compound and application thereof in synthesis of gene coding compound library |
CN115873012A (en) * | 2022-12-02 | 2023-03-31 | 中国科学院生态环境研究中心 | Biotin-perfluorocarboxylic acid compound and preparation method thereof |
CN115772174A (en) * | 2022-12-02 | 2023-03-10 | 中国科学院生态环境研究中心 | Biotin-labeled perfluorosulfonic acid compound and preparation method thereof |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2007431A1 (en) * | 1989-01-10 | 1990-07-10 | Larry W. Mclauglin | Labeling of nucleic acids with fluorescent markers |
FR2780059B1 (en) * | 1998-06-17 | 2002-10-11 | Bio Merieux | METHOD FOR LABELING A RIBONUCLEIC ACID AND BRANDED RNA FRAGMENTS THUS OBTAINED |
FR2824323B1 (en) * | 2001-05-04 | 2008-04-25 | Bio Merieux | MARKING REAGENT AND METHOD FOR DETECTING BIOLOGICAL MOLECULES |
-
2001
- 2001-05-04 FR FR0106040A patent/FR2824323B1/en not_active Expired - Fee Related
-
2002
- 2002-05-03 ES ES02740788T patent/ES2339337T3/en not_active Expired - Lifetime
- 2002-05-03 DE DE60235354T patent/DE60235354D1/en not_active Expired - Lifetime
- 2002-05-03 EP EP02740788A patent/EP1383732B1/en not_active Expired - Lifetime
- 2002-05-03 WO PCT/FR2002/001543 patent/WO2002090319A1/en active Application Filing
- 2002-05-03 JP JP2002587401A patent/JP4494717B2/en not_active Expired - Fee Related
- 2002-05-03 CN CNB028124901A patent/CN100509761C/en not_active Expired - Fee Related
- 2002-05-03 AT AT02740788T patent/ATE457973T1/en not_active IP Right Cessation
- 2002-05-03 CA CA2444918A patent/CA2444918C/en not_active Expired - Fee Related
- 2002-05-03 AU AU2002314227A patent/AU2002314227B2/en not_active Ceased
-
2004
- 2004-07-12 HK HK04105062.8A patent/HK1062014A1/en not_active IP Right Cessation
-
2008
- 2008-11-26 JP JP2008301646A patent/JP2009137954A/en active Pending
Also Published As
Publication number | Publication date |
---|---|
EP1383732A1 (en) | 2004-01-28 |
AU2002314227B2 (en) | 2008-03-20 |
ES2339337T3 (en) | 2010-05-19 |
CN1518536A (en) | 2004-08-04 |
JP4494717B2 (en) | 2010-06-30 |
CN100509761C (en) | 2009-07-08 |
EP1383732B1 (en) | 2010-02-17 |
DE60235354D1 (en) | 2010-04-01 |
WO2002090319A1 (en) | 2002-11-14 |
ATE457973T1 (en) | 2010-03-15 |
CA2444918C (en) | 2012-01-10 |
FR2824323B1 (en) | 2008-04-25 |
CA2444918A1 (en) | 2002-11-14 |
FR2824323A1 (en) | 2002-11-08 |
JP2009137954A (en) | 2009-06-25 |
JP2005502027A (en) | 2005-01-20 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PC | Patent ceased (i.e. patent has lapsed due to the failure to pay the renewal fee) |
Effective date: 20160503 |