GR1006438B - Biosensor by monitoring magnetically induced capacitance changes. - Google Patents

Biosensor by monitoring magnetically induced capacitance changes.

Info

Publication number
GR1006438B
GR1006438B GR20080100140A GR20080100140A GR1006438B GR 1006438 B GR1006438 B GR 1006438B GR 20080100140 A GR20080100140 A GR 20080100140A GR 20080100140 A GR20080100140 A GR 20080100140A GR 1006438 B GR1006438 B GR 1006438B
Authority
GR
Greece
Prior art keywords
capacitance sensor
molecular species
receptor molecules
biological receptor
target molecular
Prior art date
Application number
GR20080100140A
Other languages
Greek (el)
Inventor
ΚΑΦΕΤΖΟΠΟΥΛΟΣ (ποσοστό εξ αδιαιρέτου 30,6%) ΔΗΜΗΤΡΗΣ
Περιστερα Ανδρεακου
ΧΑΤΖΑΝΔΡΟΥΛΗΣ (ποσοστό εξ αδαιρέτου 7,5%) ΣΤΑΥΡΟΣ
Δημητρης Γουστουριδης
Χρηστος Μπουτοπουλος
Ιωαννα Ζεργιωτη
NORMAND (ποσοστό εξ αδιαιρέτου 7,5%) PASCAL
ΤΣΟΥΚΑΛΑΣ, (ποσοστό εξ αδιαιρέτου 12%) ΔΗΜΗΤΡΗΣ
Original Assignee
ΙΔΡΥΜΑ ΤΕΧΝΟΛΟΓΙΑΣ ΚΑΙ ΕΡΕΥΝΑΣ (κατά ποσοστό 20,4%)
ΕΘΝΙΚΟ ΚΕΝΤΡΟ ΕΡΕΥΝΑΣ ΦΥΣΙΚΩΝ ΕΠΙΣΤΗΜΩΝ "ΔΗΜΟΚΡΙΤΟΣ" (ποσοστό εξ αδιαιρέτου 10%)
ΚΑΦΕΤΖΟΠΟΥΛΟΣ (ποσοστό εξ αδιαιρέτου 30,6%) ΔΗΜΗΤΡΗΣ
ΖΕΡΓΙΩΤΗ (ποσοστό εξ αδιαιρέτου 12%) ΙΩΑΝΝΑ
ΧΑΤΖΑΝΔΡΟΥΛΗΣ (ποσοστό εξ αδαιρέτου 7,5%) ΣΤΑΥΡΟΣ
NORMAND (ποσοστό εξ αδιαιρέτου 7,5%) PASCAL
ΤΣΟΥΚΑΛΑΣ, (ποσοστό εξ αδιαιρέτου 12%) ΔΗΜΗΤΡΗΣ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ΙΔΡΥΜΑ ΤΕΧΝΟΛΟΓΙΑΣ ΚΑΙ ΕΡΕΥΝΑΣ (κατά ποσοστό 20,4%), ΕΘΝΙΚΟ ΚΕΝΤΡΟ ΕΡΕΥΝΑΣ ΦΥΣΙΚΩΝ ΕΠΙΣΤΗΜΩΝ "ΔΗΜΟΚΡΙΤΟΣ" (ποσοστό εξ αδιαιρέτου 10%), ΚΑΦΕΤΖΟΠΟΥΛΟΣ (ποσοστό εξ αδιαιρέτου 30,6%) ΔΗΜΗΤΡΗΣ, ΖΕΡΓΙΩΤΗ (ποσοστό εξ αδιαιρέτου 12%) ΙΩΑΝΝΑ, ΧΑΤΖΑΝΔΡΟΥΛΗΣ (ποσοστό εξ αδαιρέτου 7,5%) ΣΤΑΥΡΟΣ, NORMAND (ποσοστό εξ αδιαιρέτου 7,5%) PASCAL, ΤΣΟΥΚΑΛΑΣ, (ποσοστό εξ αδιαιρέτου 12%) ΔΗΜΗΤΡΗΣ filed Critical ΙΔΡΥΜΑ ΤΕΧΝΟΛΟΓΙΑΣ ΚΑΙ ΕΡΕΥΝΑΣ (κατά ποσοστό 20,4%)
Priority to GR20080100140A priority Critical patent/GR1006438B/en
Publication of GR1006438B publication Critical patent/GR1006438B/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
    • G01N33/6869—Interleukin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analyzing Materials By The Use Of Magnetic Means (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

According to the present invention, there is provided a method for identification, characterization, qualitative and quantitative detection of a target molecular species in a sample suspected of including said target molecular species, comprising the steps of: (i) providing at least one capacitance sensor being composed of a first flexible membrane electrode and a second counter immovable electrode, (ii) immobilizing biological receptor molecules onto the membrane electrode, (iii) bringing the sample suspected of including said target species and magnetizable label particles into contact with the capacitance sensor, said magnetizable label particles having attached binding molecules that selectively bind the target molecular species, or, in the alternative, selectively bind the immobilized biological receptor molecules and (iv) applying an external magnetic field to said at least one capacitance sensor and (v) measuring changes in capacitance, to monitor whether or not the magnetizedlabel particles have selectively bound to the biological receptor molecules.
GR20080100140A 2008-03-05 2008-03-05 Biosensor by monitoring magnetically induced capacitance changes. GR1006438B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GR20080100140A GR1006438B (en) 2008-03-05 2008-03-05 Biosensor by monitoring magnetically induced capacitance changes.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GR20080100140A GR1006438B (en) 2008-03-05 2008-03-05 Biosensor by monitoring magnetically induced capacitance changes.

Publications (1)

Publication Number Publication Date
GR1006438B true GR1006438B (en) 2009-06-16

Family

ID=40297729

Family Applications (1)

Application Number Title Priority Date Filing Date
GR20080100140A GR1006438B (en) 2008-03-05 2008-03-05 Biosensor by monitoring magnetically induced capacitance changes.

Country Status (1)

Country Link
GR (1) GR1006438B (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040058335A1 (en) * 2002-09-24 2004-03-25 Xing Su Detecting molecular binding by monitoring feedback controlled cantilever deflections
US6968744B1 (en) * 2004-10-18 2005-11-29 Silverbrook Research Pty Ltd Capacitative pressure sensor with close electrodes
US20060177874A1 (en) * 2005-02-05 2006-08-10 Yoo Kyu-Tae Sensing switch and detecting method using the same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040058335A1 (en) * 2002-09-24 2004-03-25 Xing Su Detecting molecular binding by monitoring feedback controlled cantilever deflections
US6968744B1 (en) * 2004-10-18 2005-11-29 Silverbrook Research Pty Ltd Capacitative pressure sensor with close electrodes
US20060177874A1 (en) * 2005-02-05 2006-08-10 Yoo Kyu-Tae Sensing switch and detecting method using the same

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BERGGREN C ET AL: "CAPACITIVE BIOSENSORS", ELECTROANALYSIS, VHC PUBLISHERS, INC, US, vol. 13, no. 3, 1 January 2001 (2001-01-01), pages 173 - 180, XP009027966, ISSN: 1040-0397 *
KIM ET AL.: "Micromanufacturing and Nanotechnology (book title); Nanomechanical Cantilever Devices for Biological Sensors (chapter title)", 2006, SPRINGER BERLIN HEIDELBERG, ISBN: 978-3-540-29339-2, XP002513610 *

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PG Patent granted
ML Lapse due to non-payment of fees

Effective date: 20121008