GB930720A - Cultivation of human cold virus - Google Patents

Cultivation of human cold virus

Info

Publication number
GB930720A
GB930720A GB3162/60A GB316260A GB930720A GB 930720 A GB930720 A GB 930720A GB 3162/60 A GB3162/60 A GB 3162/60A GB 316260 A GB316260 A GB 316260A GB 930720 A GB930720 A GB 930720A
Authority
GB
United Kingdom
Prior art keywords
medium
virus
concentration
plaques
incubation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB3162/60A
Inventor
David Arthur John Tyrrell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Research Development Corp UK
Original Assignee
National Research Development Corp UK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Research Development Corp UK filed Critical National Research Development Corp UK
Priority to GB3162/60A priority Critical patent/GB930720A/en
Priority to BE599028A priority patent/BE599028A/en
Priority to ES0264136A priority patent/ES264136A1/en
Priority to CH85761A priority patent/CH413235A/en
Priority to FR850929A priority patent/FR1302385A/en
Priority to DK38161A priority patent/DK102759C/en
Publication of GB930720A publication Critical patent/GB930720A/en
Expired legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/125—Picornaviridae, e.g. calicivirus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5254—Virus avirulent or attenuated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/32011—Picornaviridae
    • C12N2770/32711—Rhinovirus
    • C12N2770/32734—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/32011—Picornaviridae
    • C12N2770/32711—Rhinovirus
    • C12N2770/32751—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Virology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Chemical & Material Sciences (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

A process for the culture of the human common cold virus by incubation in a tissue culture system comprising a culture medium containing cells of human or monkey origin capable of supporting the growth of the strain or strains of virus selected is characterized in that the culture medium is maintained at a temperature of from 30 DEG to 36 DEG C. and at a pH of between 6.4 and 7.4. The vessel containing the medium and cells is rolled during incubation. A preferred pH is 6.4 to 7.2 and especially 6.8 to 7.2, the preferred temperature being 31 to 35 DEG C. and especially 32 DEG to 34 DEG C. The medium may be Hank's saline medium fortified with an enzymatic hydrolysate of lactalbumin. The medium may contain (a) an alkali metal bicarbonate in a concentration of 0.001 M and 0.01 M or a sodium phosphate buffer having a pH of 7.14 and 7.69 at a concentration of 0.05 M, (b) anti-bacterial and/or antifungal antibiotics, e.g. penicillin, streptomycin and mycostatin. After incubation for 2 to 14 days, the aqueous liquid is separated from the solids and may be used as a vaccine especially after concentration of the antigens, e.g. by ultra-centrifuging, dialysis or ion-exchange. The invention also relates to a method of testing the potency of a common cold virus preparation which comprises carrying out the culturing process of the invention, observing the cell material under a microscope at a time when a substantial number of primary plaques has been formed but before the formation of secondary plaques, counting the number of primary plaques and comparing the number of primary plaques with the number obtained in corresponding manner with a control preparation of virus.
GB3162/60A 1960-01-28 1960-01-28 Cultivation of human cold virus Expired GB930720A (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
GB3162/60A GB930720A (en) 1960-01-28 1960-01-28 Cultivation of human cold virus
BE599028A BE599028A (en) 1960-01-28 1961-01-12 Improvements in the production of intermediate products for vaccines.
ES0264136A ES264136A1 (en) 1960-01-28 1961-01-19 Procedure for the production of a material that can be used as an intermediate vaccine product or for experimental purposes (Machine-translation by Google Translate, not legally binding)
CH85761A CH413235A (en) 1960-01-28 1961-01-25 Method for cultivating human cold viruses
FR850929A FR1302385A (en) 1960-01-28 1961-01-27 Process for manufacturing intermediate substances for the preparation of vaccines, in particular against colds
DK38161A DK102759C (en) 1960-01-28 1961-01-28 Method of culturing human cold virus.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB3162/60A GB930720A (en) 1960-01-28 1960-01-28 Cultivation of human cold virus

Publications (1)

Publication Number Publication Date
GB930720A true GB930720A (en) 1963-07-10

Family

ID=9753108

Family Applications (1)

Application Number Title Priority Date Filing Date
GB3162/60A Expired GB930720A (en) 1960-01-28 1960-01-28 Cultivation of human cold virus

Country Status (1)

Country Link
GB (1) GB930720A (en)

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