GB925526A - Method of preparing true vitamin b or cyanocobalamin by fermentation process - Google Patents

Method of preparing true vitamin b or cyanocobalamin by fermentation process

Info

Publication number
GB925526A
GB925526A GB1681360A GB1681360A GB925526A GB 925526 A GB925526 A GB 925526A GB 1681360 A GB1681360 A GB 1681360A GB 1681360 A GB1681360 A GB 1681360A GB 925526 A GB925526 A GB 925526A
Authority
GB
United Kingdom
Prior art keywords
cyanocobalamin
cyanide
cobaltous
fermentation process
added
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB1681360A
Inventor
Michel Hooreman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pierrel SpA
Original Assignee
Pierrel SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pierrel SpA filed Critical Pierrel SpA
Priority to GB1681360A priority Critical patent/GB925526A/en
Publication of GB925526A publication Critical patent/GB925526A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/42—Cobalamins, i.e. vitamin B12, LLD factor

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Cyanocobalamin, or a concentrate containing a major proportion of cyanocobalamin is produced by a direct fermentation process in which cobaltous cyanide is added to the nutrient medium and the concentration of cobaltous and cyanide ions is kept constant throughout the fermentation. Preferably sterile cyanide is added to the medium after it has been sterilized to avoid supersaturation. In example Propionibacterium is grown on a medium containing cornsteep, yeast autolysate, casein hydrolysate and glucose and from 20 to 100 p.p.m. of cobaltous cyanide at 30 DEG . using moderate agitation. After fermentation for up to 84 hours the mass was centrifuged, washed and dried by atomization. The product consists almost wholly of cyanocobalamin with a very small proportion of hydroxy cobalamin. Specifications 695,273 and 699,369 are referred to.ALSO:A powder containing 700 mg./kg. of cyanocobalamin and less than 50 mg./kg. of hydroxycobalamin is a growth promoting feed for cattle. The powder may be made by a direct fermentation process for cyanocobalamin in which cobaltous cyanide has been added to the nutrient medium (see Group IV (b)). Specifications 695,273 and 699,369 are referred to.
GB1681360A 1960-05-12 1960-05-12 Method of preparing true vitamin b or cyanocobalamin by fermentation process Expired GB925526A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB1681360A GB925526A (en) 1960-05-12 1960-05-12 Method of preparing true vitamin b or cyanocobalamin by fermentation process

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB1681360A GB925526A (en) 1960-05-12 1960-05-12 Method of preparing true vitamin b or cyanocobalamin by fermentation process

Publications (1)

Publication Number Publication Date
GB925526A true GB925526A (en) 1963-05-08

Family

ID=10084130

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1681360A Expired GB925526A (en) 1960-05-12 1960-05-12 Method of preparing true vitamin b or cyanocobalamin by fermentation process

Country Status (1)

Country Link
GB (1) GB925526A (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004044215A2 (en) * 2002-11-14 2004-05-27 Dsm Ip Assets B.V. Formulations comprising vitamin b12, method of production and use thereof
DE102013100891A1 (en) 2013-01-29 2014-07-31 Erdinger Weissbräu Franz Brombach e.K. Process for the preparation of an acid, a mash or wort, a drink, a concentrate and the products produced therewith and use of lactic acid bacteria, an acid, a mash, a wort or a concentrate
DE102013008336A1 (en) 2013-01-29 2014-07-31 Erdinger Weissbräu Franz Brombach e.K. Producing brewing malt, involves providing malting raw material, preferably grain including barley or wheat, and treating raw material, preferably grain with lactic acid bacteria of strain Lactobacillus coryniformis
DE102013008335A1 (en) 2013-01-29 2014-07-31 Erdinger Weissbräu Franz Brombach e.K. Process for the preparation of a food as well as the food produced therewith and use of lactic acid bacteria, an acid, a mash, a wort, a drink or a concentrate
DE102014110182A1 (en) 2014-07-18 2016-01-21 Erdinger Weißbräu Werner Brombach GmbH & Co. KG Process for the preparation of a food or a precursor thereof, food or a precursor thereof and corresponding use

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004044215A2 (en) * 2002-11-14 2004-05-27 Dsm Ip Assets B.V. Formulations comprising vitamin b12, method of production and use thereof
WO2004044215A3 (en) * 2002-11-14 2004-08-26 Dsm Ip Assets Bv Formulations comprising vitamin b12, method of production and use thereof
DE102013100891A1 (en) 2013-01-29 2014-07-31 Erdinger Weissbräu Franz Brombach e.K. Process for the preparation of an acid, a mash or wort, a drink, a concentrate and the products produced therewith and use of lactic acid bacteria, an acid, a mash, a wort or a concentrate
DE102013008336A1 (en) 2013-01-29 2014-07-31 Erdinger Weissbräu Franz Brombach e.K. Producing brewing malt, involves providing malting raw material, preferably grain including barley or wheat, and treating raw material, preferably grain with lactic acid bacteria of strain Lactobacillus coryniformis
DE102013008335A1 (en) 2013-01-29 2014-07-31 Erdinger Weissbräu Franz Brombach e.K. Process for the preparation of a food as well as the food produced therewith and use of lactic acid bacteria, an acid, a mash, a wort, a drink or a concentrate
WO2014118190A1 (en) 2013-01-29 2014-08-07 Erdinger Weissbräu Franz Brombach e.K. Method for producing an acid malt, an acid malt, method for producing a mash or wort, mash or wort, method for producing a drink, a drink, method for producing a concentrate, a concentrate
WO2014118191A1 (en) 2013-01-29 2014-08-07 Erdinger Weissbräu Franz Brombach e.K. Method for producing a foodstuff and the thus produced foodstuff and use of lactic acid bacteria, acid malt, a mash, a wort, a drink or a concentrate
DE102014110182A1 (en) 2014-07-18 2016-01-21 Erdinger Weißbräu Werner Brombach GmbH & Co. KG Process for the preparation of a food or a precursor thereof, food or a precursor thereof and corresponding use
EP2995204A1 (en) 2014-07-18 2016-03-16 Erdinger Weißbräu Werner Brombach GmbH & Co. KG Method for the preparation of a food or a precursor of the same, food or a precursor of the same, and uses of same

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