GB911186A - Improvements in or relating to b.c.g. vaccines - Google Patents
Improvements in or relating to b.c.g. vaccinesInfo
- Publication number
- GB911186A GB911186A GB22616/60A GB2261660A GB911186A GB 911186 A GB911186 A GB 911186A GB 22616/60 A GB22616/60 A GB 22616/60A GB 2261660 A GB2261660 A GB 2261660A GB 911186 A GB911186 A GB 911186A
- Authority
- GB
- United Kingdom
- Prior art keywords
- freeze
- medium
- organisms
- dried
- nutrient
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
- A61K39/04—Mycobacterium, e.g. Mycobacterium tuberculosis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Communicable Diseases (AREA)
- Pulmonology (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A process for the production of a freeze-dried B.C.G. vaccine in which B.C.G. organisms (Bacillus of Calmette and Guorin) are cultured in a nutrient medium therefor and thereafter freeze-dried, is characterized in that the said nutrient medium is one substantially free from glycerol, monosaccharides, sugar alcohols and polysaccharides containing a carbonyl group or metabolized by the organisms to a carbonyl-group containing compound. Preferably, the culture is submerged, and the medium contains (a) at least one amino acid and/or an enzymatic digest of casein and/or a meat protein hydrolysate and/or a meat extract to provide essential nitrogen and carbon requirements; (b) a non-hydrolysable non-ionic wetting agent e.g. a polyoxyethylene ether of a phenol or long chain alcohol; (c) nutrient salts i.e. salts of iron, calcium, zinc and copper; (d) a buffer e.g. disodium hydrogen phosphate/potassium dihydrogen phosphate to pH 5.5 to 8.5 and preferably pH 7.0. The culture medium preferably contains L-asparagine as amino acid and may also contain monosodium glutamate and/or L-glutamine. After cultivation for 8-21 days at 35 DEG to 39 DEG C., the organisms are separated from the liquid medium by centrifugation. The organisms are then re-suspended in a freeze-drying medium and freeze-dried. The preferred freeze-drying medium consisting of an aqueous solution of dextran containing a wetting agent and glucose with or without the further addition of monosodium glutamate. Specification 764,718 is referred to.
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB22616/60A GB911186A (en) | 1960-06-28 | 1960-06-28 | Improvements in or relating to b.c.g. vaccines |
BE605435A BE605435A (en) | 1960-06-28 | 1961-06-26 | Improved process for the preparation of the B.C.G. |
FR866237A FR1415M (en) | 1960-06-28 | 1961-06-27 | Vaccine type b.c.g. |
DEG32603A DE1148038B (en) | 1960-06-28 | 1961-06-28 | Process for vaccine production |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB22616/60A GB911186A (en) | 1960-06-28 | 1960-06-28 | Improvements in or relating to b.c.g. vaccines |
Publications (1)
Publication Number | Publication Date |
---|---|
GB911186A true GB911186A (en) | 1962-11-21 |
Family
ID=10182328
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB22616/60A Expired GB911186A (en) | 1960-06-28 | 1960-06-28 | Improvements in or relating to b.c.g. vaccines |
Country Status (4)
Country | Link |
---|---|
BE (1) | BE605435A (en) |
DE (1) | DE1148038B (en) |
FR (1) | FR1415M (en) |
GB (1) | GB911186A (en) |
-
1960
- 1960-06-28 GB GB22616/60A patent/GB911186A/en not_active Expired
-
1961
- 1961-06-26 BE BE605435A patent/BE605435A/en unknown
- 1961-06-27 FR FR866237A patent/FR1415M/en active Active
- 1961-06-28 DE DEG32603A patent/DE1148038B/en active Pending
Also Published As
Publication number | Publication date |
---|---|
BE605435A (en) | 1961-10-16 |
DE1148038B (en) | 1963-05-02 |
FR1415M (en) | 1962-07-23 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Schimke et al. | The generation of energy by the arginine dihydrolase pathway in Mycoplasma hominis 07 | |
Schimke et al. | Arginine metabolism in pleuropneumonia-like organisms isolated from mammalian cell culture | |
DeNiro et al. | Mechanism of carbon isotope fractionation associated with lipid synthesis | |
Simpson et al. | DEGRADATION OF l-ARABINOSE BY AEROBACTER AEROGENES: IA PATHWAY INVOLVING PHOSPHORYLATED INTERMEDIATES | |
Mangum et al. | Vitamin B12 dependent methionine biosynthesis in cultured mammalian cells | |
Roy et al. | Effect of glycine on the survival of bull spermatozoa in vitro | |
Gale | Determination of amino acids by use of bacterial amino acid decarboxylases | |
Neuberg | The biochemistry of yeast | |
US3135663A (en) | Vaccines | |
GB911186A (en) | Improvements in or relating to b.c.g. vaccines | |
Virtanen | On the enzymes of bacteria and bacterial metabolism | |
Natake et al. | Studies on glucose isomerase of bacteria: Part I. Formation of glucose isomerase by Aerobacter aerogenes, Strain HN-56, and its relationship to xylose isomerase | |
Lee et al. | Purification and reaction mechanism of arylsulfate sulfotransferase from Haemophilus K-12, a mouse intestinal bacterium | |
Corpe et al. | Oxidation of glycolic acid by Penicillium chrysogenum | |
GB842679A (en) | Process for producing l-glutamic acid | |
MX2955E (en) | IMPROVED METHOD FOR THE PRODUCTION OF STREPTOKINASE | |
SASAKI et al. | On the mechanism of anaerobic formation of succinate by Proteus vulgaris | |
Gillespie | Properties of Wool Root Phosphatases | |
Dingle et al. | Inhibition of Pyruvate Metabolism in Mitochondria Suspensions by Cobalt | |
GB951299A (en) | Method for producing vaccine concentrates of brucella abortus organisms | |
GB981273A (en) | A method for preparing 5-keto fructose by fermentation | |
Mahlen et al. | A metabolic variation in Penicillium spiculisporum Lehman | |
Takahashi et al. | Lipase formation by micro-organisms grown on hydrocarbons | |
Shah et al. | Studies on glutamic acid decarboxylase from Listeria monocytogenes. | |
GB930720A (en) | Cultivation of human cold virus |