GB9106007D0 - Improvements in,or relating to,volume measurement of microbial organisms - Google Patents

Improvements in,or relating to,volume measurement of microbial organisms

Info

Publication number
GB9106007D0
GB9106007D0 GB9106007A GB9106007A GB9106007D0 GB 9106007 D0 GB9106007 D0 GB 9106007D0 GB 9106007 A GB9106007 A GB 9106007A GB 9106007 A GB9106007 A GB 9106007A GB 9106007 D0 GB9106007 D0 GB 9106007D0
Authority
GB
Grant status
Application
Patent type
Prior art keywords
relating
improvements
volume measurement
microbial organisms
image
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB9106007A
Other versions
GB2254414A (en )
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
London University College
Original Assignee
London University College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Electro-optical investigation, e.g. flow cytometers
    • G01N15/1468Electro-optical investigation, e.g. flow cytometers with spatial resolution of the texture or inner structure of the particle
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements

Abstract

A method and apparatus for cell volume and biomass estimation by sampling a fermentation broth and analyzing an image of the broth using brightness thresholds to distinguish between background, cellular regions and cytoplasmic and degenerated regions and summing corresponding areas in the image. Volumes can be calculated from the areas by treating the organisms as geometrical bodies and biomass derived by multiplying the volume by a density.
GB9106007A 1991-03-21 1991-03-21 Volume measurement of microbial organisms. Withdrawn GB2254414A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB9106007A GB2254414A (en) 1991-03-21 1991-03-21 Volume measurement of microbial organisms.

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB9106007A GB2254414A (en) 1991-03-21 1991-03-21 Volume measurement of microbial organisms.
JP50655192A JPH06508021A (en) 1991-03-21 1992-03-20
EP19920907138 EP0575450A1 (en) 1991-03-21 1992-03-20 Improvements in, or relating to, biomass and differentiation measurements on microbial organisms
PCT/GB1992/000508 WO1992016614A1 (en) 1991-03-21 1992-03-20 Improvements in, or relating to, biomass and differentiation measurements on microbial organisms

Publications (2)

Publication Number Publication Date
GB9106007D0 true true GB9106007D0 (en) 1991-05-08
GB2254414A true GB2254414A (en) 1992-10-07

Family

ID=10691956

Family Applications (1)

Application Number Title Priority Date Filing Date
GB9106007A Withdrawn GB2254414A (en) 1991-03-21 1991-03-21 Volume measurement of microbial organisms.

Country Status (4)

Country Link
EP (1) EP0575450A1 (en)
JP (1) JPH06508021A (en)
GB (1) GB2254414A (en)
WO (1) WO1992016614A1 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10347123B4 (en) 2003-10-02 2005-07-28 Petra Dr.-Ing. Perner A method for automatically determining on a support collected airborne particles especially fungal spores
WO2006057444A1 (en) * 2004-11-25 2006-06-01 National University Corporation Hokkaido University Method of automatically evaluating differentiation degree of cells
JP5332610B2 (en) * 2006-05-22 2013-11-06 株式会社ニコン Cell detachment determination device, cell detachment determination method, and cell culture apparatus
JP5239128B2 (en) 2006-05-22 2013-07-17 株式会社ニコン Observation device
ES2464572T3 (en) 2008-03-21 2014-06-03 Abbott Point Of Care, Inc. Method and apparatus for determining red blood cell indices in a blood sample using the intrinsic pigmentation hemoglobin in red blood cells
JP5184697B2 (en) 2008-03-21 2013-04-17 アボット ポイント オブ ケア インコーポレイテッド Individually platelets, and detection and a method and apparatus for counting the agglomerates
JP5082010B2 (en) 2008-03-21 2012-11-28 アボット ポイント オブ ケア インコーポレイテッド By utilizing the inherent dye of hemoglobin contained in red blood cells the method and apparatus for measuring hematocrit of a blood sample
EP2260343B1 (en) 2008-03-21 2016-09-21 Abbott Point Of Care, Inc. Method and apparatus for determining a focal position of an imaging device adapted to image a biologic sample
WO2009117683A3 (en) 2008-03-21 2009-11-19 Abbott Point Of Care Method and apparatus for analyzing individual cells or particulates using fluorescent quenching and/or bleaching
JP4976586B2 (en) 2008-04-02 2012-07-18 アボット ポイント オブ ケア インコーポレイテッド Gradient diluter implemented in self-calibrating gradient dilution and thin film samples in component assay
EP2269033B1 (en) 2008-04-09 2016-09-21 Abbott Point Of Care, Inc. Method for measuring the area of a sample disposed within an analysis chamber
WO2011082342A1 (en) 2009-12-31 2011-07-07 Abbott Point Of Care, Inc. Method and apparatus for determining mean cell volume of red blood cells
WO2011116305A1 (en) 2010-03-18 2011-09-22 Abbott Point Of Care, Inc. Method and apparatus for optically determining at least one hemoglobin related parameter of a whole blood sample
JP2016116461A (en) * 2014-12-19 2016-06-30 パナソニック株式会社 Cell determination method, and cell number measurement method

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1385679A (en) * 1971-04-17 1975-02-26 Image Analysing Computers Ltd Density measurement by image analysis
US4045655A (en) * 1973-10-15 1977-08-30 Hitachi, Ltd. Automatic cyto-screening device
DE2515513B2 (en) * 1975-04-09 1977-07-07 A method of measuring amounts of substance samples into light transmitting
US4453266A (en) * 1980-04-21 1984-06-05 Rush-Presbyterian-St. Luke's Medical Center Method and apparatus for measuring mean cell volume of red blood cells
DE3211314C2 (en) * 1982-03-26 1987-04-23 Vsesojuznyj Naucno-Issledovatel'skij Institut Biosinteza Belkovych Vescestv, Moskau/Moskva, Su
JPH07114685B2 (en) * 1983-07-28 1995-12-13 株式会社日立製作所 Sewage treatment equipment
FR2555754A1 (en) * 1983-11-28 1985-05-31 Inter Inf Method and automatic analyzing device for biological samples

Also Published As

Publication number Publication date Type
WO1992016614A1 (en) 1992-10-01 application
EP0575450A1 (en) 1993-12-29 application
JPH06508021A (en) 1994-09-14 application
GB2254414A (en) 1992-10-07 application

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Legal Events

Date Code Title Description
WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)