GB886712A - Improvements in or relating to the preparation of erythropoietic factor concentrates - Google Patents

Abstract

An erythropoietic factor is concentrated by contacting a solution of anaemic plasma with an ion exchange material and eluting the sorbed extract therefrom. The ion exchange material may be an insoluble, substantially open-chain polymer such as a polysaccharide derivative; specified polysaccharides are starches (e.g. amylose and amylopectin), inulins, cellulose (e.g. a - and oxy-celluloses), xylans, pectine and algins, glycogens, chitins and muco polysaccharides (e.g. chondroitin sulphuric acid, heparin and hyaluronic acid), levans and dextrans. Particularly preferred ion-exchangers are the anion-exchangers cellulose-dimethylaminoethyl- or diethylaminoethyl-ethers (prepared by condensing sodium cellulose with 2-chloroethyl-dimethylamine or -diethylamine respectively); carboxyf methylcellulose, cellulose citrate (esterification oan a -cellulose with citric acid) and cellulose phosphate are mentioned as cation-exchangers. The anaemic plasma may be from ovine, bovine, porcine or other mammalian sources, but sheep plasma is preferred. The plasma solution preferably has a pH of 3.5-8.5, particularly 4.0-6.0, e.g. 4.2-4.8, and an alkali metal salt concentration of 0.01-0.06M, e.g. 0.03-0.04M NaCl (in the example NaH2PO4 is also present). Elution is preferably effected with a 0.1-0.5M NaCl solution with a pH below 7.0. Details are given of biological assay of the product.ALSO:An erythropoietic factor is concentrated by contacting a solution of anaemic plasma with an ion exchange material and eluting the sorbed extract therefrom. The ion exchange material may be an insoluble, substantially open-chain polymer such as a polysaccharide derivative; specified polysaccharides are starches (e.g. amylose and amylopectin), inulins, cellulose (e.g. a - and oxy-celluloses), xylans, pectine and algins, glycogens, chitins and muco polysaccharides (e.g. chondroitin sulphuric acid, heparin and hyaluronic acid), levans and dextrans. Particularly preferred ion-exchangers are the anion-exchangers cellulose-dimethylaminoethyl- or diethylaminoethyl-ethers (prepared by condensing sodium cellulose with 2-chloroethyl-dimethylamine or -diethylamine respectively); carboxymethylcellulose, cellulose citrate (esterification of an -cellulose with citric acid) and cellulose phosphate are mentioned as cation-exchangers. The anaemic plasma may be from ovine, p bovine, porcine or other mammalian sources, but sheep plasma is preferred. The plasma solution preferably has a pH of 3.5-8.5, particularly 4.0-6.0, e.g. 4.2-4.8, and an alkali metal salt concentration of 0.01-0.06 M, e.g. 0.03-0.04 M NaCl (in the example NaH2PO4 is also present). Elution is preferably effected with a 0.1-0.5 M NaCl solution with a pH below 7.0. Details are given of a biological assay of the product.