GB886553A - Substitutes for blood plasma and process for their manufacture - Google Patents
Substitutes for blood plasma and process for their manufactureInfo
- Publication number
- GB886553A GB886553A GB2491/59A GB249159A GB886553A GB 886553 A GB886553 A GB 886553A GB 2491/59 A GB2491/59 A GB 2491/59A GB 249159 A GB249159 A GB 249159A GB 886553 A GB886553 A GB 886553A
- Authority
- GB
- United Kingdom
- Prior art keywords
- hydrolysate
- cross
- solution
- yield
- reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 210000002381 plasma Anatomy 0.000 title abstract 3
- 238000004519 manufacturing process Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 239000000413 hydrolysate Substances 0.000 abstract 14
- 238000006243 chemical reaction Methods 0.000 abstract 12
- 239000007857 degradation product Substances 0.000 abstract 8
- 239000012948 isocyanate Substances 0.000 abstract 8
- 150000002513 isocyanates Chemical class 0.000 abstract 8
- 230000011382 collagen catabolic process Effects 0.000 abstract 6
- 125000002795 guanidino group Chemical group C(N)(=N)N* 0.000 abstract 6
- RRAMGCGOFNQTLD-UHFFFAOYSA-N hexamethylene diisocyanate Chemical compound O=C=NCCCCCCN=C=O RRAMGCGOFNQTLD-UHFFFAOYSA-N 0.000 abstract 6
- 230000003301 hydrolyzing effect Effects 0.000 abstract 6
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 abstract 6
- 239000005057 Hexamethylene diisocyanate Substances 0.000 abstract 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 abstract 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 4
- 230000015556 catabolic process Effects 0.000 abstract 4
- 238000004132 cross linking Methods 0.000 abstract 4
- 238000006731 degradation reaction Methods 0.000 abstract 4
- 229920000159 gelatin Polymers 0.000 abstract 4
- 235000019322 gelatine Nutrition 0.000 abstract 4
- 108010010803 Gelatin Proteins 0.000 abstract 3
- 239000008273 gelatin Substances 0.000 abstract 3
- 235000011852 gelatine desserts Nutrition 0.000 abstract 3
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 102000057297 Pepsin A Human genes 0.000 abstract 2
- 108090000284 Pepsin A Proteins 0.000 abstract 2
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 abstract 2
- 102000004142 Trypsin Human genes 0.000 abstract 2
- 108090000631 Trypsin Proteins 0.000 abstract 2
- 230000002378 acidificating effect Effects 0.000 abstract 2
- 125000001931 aliphatic group Chemical group 0.000 abstract 2
- 125000003118 aryl group Chemical group 0.000 abstract 2
- 229940088598 enzyme Drugs 0.000 abstract 2
- 230000007062 hydrolysis Effects 0.000 abstract 2
- 238000006460 hydrolysis reaction Methods 0.000 abstract 2
- 229940111202 pepsin Drugs 0.000 abstract 2
- 239000000047 product Substances 0.000 abstract 2
- 239000011833 salt mixture Substances 0.000 abstract 2
- 239000011780 sodium chloride Substances 0.000 abstract 2
- 239000000725 suspension Substances 0.000 abstract 2
- 210000001519 tissue Anatomy 0.000 abstract 2
- 239000012588 trypsin Substances 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- 239000001828 Gelatine Substances 0.000 abstract 1
- -1 hydro-aromatic Chemical group 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
A substitute for blood plasma is produced by (i) subjecting a collagen degradation product to mild hydrolytic degradation to yield a hydrolysate of molecular weight of 2,000 to 20,000, e.g. 5,000 to 10,000, cross-linking the hydrolysate at pH about 7 by reaction with a proportion of a polyfunctional isocyanate smaller than that calculated for the total reaction of all the amino and guanidino groups present in the hydrolysate; (ii) cross-linking a collagen degradation product at pH about 7 by reaction with a proportion of a polyfunctional isocyanate smaller than that calculated for the total reaction of all the amino and guanidino groups present, subjecting the cross-linked product to mild hydrolytic degradation to yield a hydrolysate having a molecular weight of 10,000 to 100,000, e.g. 30,000 to 60,000; and the solution obtained from (i) or (ii) is adjusted to pH about 7, if it does not have that value, and is rendered isotonic. The collagen degradation product may be gelatin. The proportion of the isocyanate may amount to 20% to 80% of that calculated for the total reaction of all the amino and guanidino groups present. The isocyanate may be aromatic, hydroaromatic or aliphatic of formula OCN-(CH2)x-NCO where X is a whole number from 2 to 20, e.g. hexamethylene, diisocyanate, which may be used in solution or suspension in tetrahydrofurane, acetone or water. The concentration of the cross-linked hydrolytic degradation product in solution may be 4% to 6% and the solution may be rendered isotonic by addition of sodium chloride or physiological salt mixture. The hydrolysis may be acidic, alkaline, or fermentative, e.g. using trypsin, pepsin and tissue enzymes. An aqueous gelatin solution of 5% strength may be (i) hydrolysed under pressure at 120 DEG C. to yield a hydrolysate having a molecular weight of 5,000 to 10,000 and the hydrolysate cross-linked with hexamethylene diisocyanate at 20 DEG C. to 30 DEG C., or (ii) may be cross-linked by reaction with hexamethylene diisocyanate at 20 DEG C. to 30 DEG C. and subsequently hydrolysed under pressure at 120 DEG C. to yield a hydrolysate having a molecular weight of 10,000 to 100,000.ALSO:A substitute for blood plasma is produced by (i) subjecting a collagen degradation product to mild hydrolytic degradation to yield a hydrolysate of molecular weight of 2000 to 20,000, e.g. 5000 to 10,000, cross-linking the hydrolysate at pH about 7 by reaction with a proportion of a polyfunctional isocyanate smaller than that calculated for the total reaction of all the amino and guanidino groups present in the hydrolysate; or (ii) cross-linking a collagen degradation product at pH about 7 by reaction with a proportion of a polyfunctional isocyanate smaller than that calculated for the total reaction of all the amino or guanidino groups present, subjecting the cross-linked product to mild hydrolytic degradation to yield a hydrolysate having a molecular weight of 10,000 to 100,000, e.g. 30,000 to 60,000; and the solution obtained from (i) or (ii) is adjusted to pH about 7, if it does not have that value, and is rendered isotonic. The collagen degradation product may be gelatin. The proportion of the isocyanate may amount to 20 to 80% of that calculated for the total reaction of all the amino and guanidino groups present. The isocyanate may be aromatic, hydro-aromatic, or aliphatic of formula OCN-(CH2)x-NCO where x is a whole number from 2 to 20, e.g. hexamethylene diisocyanate, which may be used in solution or suspension in tetrahydrofurane, acetone or water. The concentration of the cross-linked hydrolytic degradation product in solution may be 4 to 6% and the solution may be rendered isotonic by addition of sodium chloride or physiological salt mixture. The hydrolysis may be acidic, alkaline, or fermentative, e.g. using trypsin, pepsin and tissue enzymes. An aqueous gelatine solution of 5% strength may be (i) hydrolysed under pressure at 120 DEG C. to yield a hydrolysate having a molecular weight of 5000 to 10,000 and the hydrolysate cross-linked with hexamethylene diisocyanate at 20 DEG to 30 DEG C., or (ii) may be cross-linked by reaction with hexamethylene diisocyanate at 20 DEG to 30 DEG C. and subsequently hydrolysed under pressure at 120 DEG C. to yield a hydrolysate having a molecular weight of 10,000 to 100,000.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE886553X | 1958-01-22 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB886553A true GB886553A (en) | 1962-01-10 |
Family
ID=6833378
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB2491/59A Expired GB886553A (en) | 1958-01-22 | 1959-01-22 | Substitutes for blood plasma and process for their manufacture |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB886553A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3239420A (en) * | 1962-10-11 | 1966-03-08 | Vitamix Pharmaceuticals Inc | Gelatin composition |
FR2042381A1 (en) * | 1969-04-28 | 1971-02-12 | Knox Gelatine Inc | |
DE3138094A1 (en) * | 1980-09-25 | 1982-05-06 | Yamanouchi Pharmaceutical Co., Ltd., Tokyo | AGENT FOR IMPROVING BLOOD CIRCULATION |
-
1959
- 1959-01-22 GB GB2491/59A patent/GB886553A/en not_active Expired
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3239420A (en) * | 1962-10-11 | 1966-03-08 | Vitamix Pharmaceuticals Inc | Gelatin composition |
FR2042381A1 (en) * | 1969-04-28 | 1971-02-12 | Knox Gelatine Inc | |
DE3138094A1 (en) * | 1980-09-25 | 1982-05-06 | Yamanouchi Pharmaceutical Co., Ltd., Tokyo | AGENT FOR IMPROVING BLOOD CIRCULATION |
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