GB883766A - Improvements in or relating to columns for chromatography - Google Patents

Improvements in or relating to columns for chromatography

Info

Publication number
GB883766A
GB883766A GB2134959A GB2134959A GB883766A GB 883766 A GB883766 A GB 883766A GB 2134959 A GB2134959 A GB 2134959A GB 2134959 A GB2134959 A GB 2134959A GB 883766 A GB883766 A GB 883766A
Authority
GB
United Kingdom
Prior art keywords
cellulose
reel
paper
aqueous
solvent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB2134959A
Inventor
Pauline Mary Pownall
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
W & R Balston Ltd
Original Assignee
W & R Balston Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by W & R Balston Ltd filed Critical W & R Balston Ltd
Priority to GB2134959A priority Critical patent/GB883766A/en
Publication of GB883766A publication Critical patent/GB883766A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6052—Construction of the column body
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/52—Physical parameters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/52—Physical parameters
    • G01N2030/522—Physical parameters pressure
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6052—Construction of the column body
    • G01N30/6069—Construction of the column body with compartments or bed substructure

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Abstract

A chromatographic column for use with solvents causing cellulose to swell comprises a rigid core (1) having a paper strip wound thereon to form a reel (2) functioning as the separation medium, and surrounded by a close-fitting flexible sleeve (3) in turn having a paper strip <PICT:0883766/III/1> wound thereon to form a reel (4) surrounded by a close-fitting flexible sleeve (5). In use, the swelling solvent is applied to both reels and the second reel functions to reduce pressure inequalities in the first reel as a result of swelling. The numerals relate to the Figure in which eluted liquid is collected via funnel 6 in vessel 9 and a drip tray 7 is provided for liquid which has passed through the second reel. The rigid core may be made of polyethylene or glass; the sleeves are also preferably made of polyethylene. Paper having ion exchange properties may be employed, e.g. paper comprising one or more ion exchange resins (as in Specification 814,363) or a paper prepared from carboxymethyl cellulose, aminoethyl cellulose, diethylaminoethyl cellulose, 3(N:N diethanolaminoethoxy) 2(hydroxy) propyl cellulose, cellulose citrate, or cellulose phosphate. Examples mention the separation of an aqueous HCl solution of amino-acids using either an aqueous methyl ethyl ketone/propionic acid mixture or an aqueous sodium acetate/HCl buffer solution as developing solvent and the separation of methyl orange and xylene cyanol using water solvent. Aqueous electrolyte/organic solvent developing solutions may also be used.
GB2134959A 1959-06-22 1959-06-22 Improvements in or relating to columns for chromatography Expired GB883766A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB2134959A GB883766A (en) 1959-06-22 1959-06-22 Improvements in or relating to columns for chromatography

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB2134959A GB883766A (en) 1959-06-22 1959-06-22 Improvements in or relating to columns for chromatography

Publications (1)

Publication Number Publication Date
GB883766A true GB883766A (en) 1961-12-06

Family

ID=10161393

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2134959A Expired GB883766A (en) 1959-06-22 1959-06-22 Improvements in or relating to columns for chromatography

Country Status (1)

Country Link
GB (1) GB883766A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2690435B1 (en) * 2011-03-22 2022-10-05 Sekisui Medical Co., Ltd. Liquid chromatography column, and method for analyzing hemoglobin

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2690435B1 (en) * 2011-03-22 2022-10-05 Sekisui Medical Co., Ltd. Liquid chromatography column, and method for analyzing hemoglobin

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