GB625820A - Purification of antibiatic substances - Google Patents
Purification of antibiatic substancesInfo
- Publication number
- GB625820A GB625820A GB17671/46A GB1767146A GB625820A GB 625820 A GB625820 A GB 625820A GB 17671/46 A GB17671/46 A GB 17671/46A GB 1767146 A GB1767146 A GB 1767146A GB 625820 A GB625820 A GB 625820A
- Authority
- GB
- United Kingdom
- Prior art keywords
- salt
- column
- antibiotic
- solvent
- fractions
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Landscapes
- Compounds Of Unknown Constitution (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Water-soluble basic antibiotic substances elaborated by micro-organisms of the genus Actinomyces are purified by dissolving an acid salt thereof (e.g. a hydrohalide, picrate or lower aliphatic carboxylic acid salt of streptothricin or streptomycin) in a solvent for such salt or a solvent mixture in which it is at least moderately soluble, adding the solution to a column of adsorbent material, washing the column with a solvent or solvent mixture for the salt to develop zones of adsorbent material containing the antibiotic substance in differing amounts and degrees of purity, and selectively recovering the antibiotic substance from these zones. Suitable adsorbents are charcoal, alumina, diatomaceous silica and especially acid washed charcoal and acid washed alumina, e.g. the product obtained by treating aluminium oxide with sulphuric acid, washing with water until the pH of the washings is about 4.5 and drying at elevated temperature. The column, which may contain 10-30 grams of adsorbent for each gram of antibiotic salt to be treated, is preferably of the chromatographic type and preferably wet packed, and if desired the rate of flow of eluting solvent can be modified by adding a material such as filter paper pulp to the adsorbent. Suitable solvents for the preparation of the original solution, and for the development and elution (through the two need not be identical), are water, lower aliphatic alcohols and mixtures thereof, or, for somewhat known solubility (which is sometimes advantageous in the adsorption step), mixtures of these solvents with acetone or chloroform, and the concentration of the initial solution is advantageously about 10-50 per cent. When the adsorption step is completed, fresh solvent is added to the top of the column and the eluate from the bottom is collected and tested until solids are found to be present, after which the eluate is collected in fractions which may be arbitrarily chosen or empirically predetermined or, in the case of coloured salts such as picrates, may be determined by observing the movement of coloured bands down the column. Alternatively, after development, the column is freed from solvent and divided into separate sections known or predetermined to contain optimum or predominant amounts of antibiotic salt, which is extracted therefrom with a solvent. The distribution of antibiotic salt in the column differs with different compounds, streptothricin salts being mainly found in later fractions of eluate or higher zones of the column (with impurities in the earlier fractions or lower zones), whereas with streptomycin salts the purer salt is generally found in the early fractions or lower zones. The recovery of the antibiotic salt from the various eluate fractions or extracts may be effected by partially concentrating them, either separately or combined, and mixing them with a miscible solvent in which the antibiotic is insoluble, e.g. acetone, ether or mixtures thereof, causing precipitation of the antibiotic salt. Alternatively the concentrates may be subjected to a further purification by adsorption and elution. Examples describe the purification of streptothricin hydrochloride and picrate and streptomycin hydrochloride. Specification 625,821 is referred to.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US625820XA | 1945-06-23 | 1945-06-23 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB625820A true GB625820A (en) | 1949-07-05 |
Family
ID=22043420
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB17671/46A Expired GB625820A (en) | 1945-06-23 | 1946-06-12 | Purification of antibiatic substances |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB625820A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
TWI510276B (en) * | 2011-05-20 | 2015-12-01 | Univ Nat Central | Peptide chromatographic purification assisted by combining of solubility parameter and solution conformation energy calculations |
-
1946
- 1946-06-12 GB GB17671/46A patent/GB625820A/en not_active Expired
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
TWI510276B (en) * | 2011-05-20 | 2015-12-01 | Univ Nat Central | Peptide chromatographic purification assisted by combining of solubility parameter and solution conformation energy calculations |
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