GB2617285A - Multiparametric discovery and optimization platform - Google Patents

Multiparametric discovery and optimization platform Download PDF

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Publication number
GB2617285A
GB2617285A GB2308819.8A GB202308819A GB2617285A GB 2617285 A GB2617285 A GB 2617285A GB 202308819 A GB202308819 A GB 202308819A GB 2617285 A GB2617285 A GB 2617285A
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United Kingdom
Prior art keywords
cells
polymer
polymer variant
sample
variant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB2308819.8A
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English (en)
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GB202308819D0 (en
Inventor
Agresti Jeremy
Ornelas Vargas Andres
Gregory Hoff Kevin
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Triplebar Bio Inc
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Triplebar Bio Inc
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Publication of GB202308819D0 publication Critical patent/GB202308819D0/en
Publication of GB2617285A publication Critical patent/GB2617285A/en
Withdrawn legal-status Critical Current

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    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1089—Design, preparation, screening or analysis of libraries using computer algorithms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G16B40/20—Supervised data analysis
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/50—Mutagenesis
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B35/00—ICT specially adapted for in silico combinatorial libraries of nucleic acids, proteins or peptides
    • G16B35/10—Design of libraries
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B99/00—Subject matter not provided for in other groups of this subclass

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Molecular Biology (AREA)
  • Medical Informatics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Theoretical Computer Science (AREA)
  • Evolutionary Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Analytical Chemistry (AREA)
  • Data Mining & Analysis (AREA)
  • Ecology (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Artificial Intelligence (AREA)
  • Bioethics (AREA)
  • Databases & Information Systems (AREA)
  • Epidemiology (AREA)
  • Evolutionary Computation (AREA)
  • Public Health (AREA)
  • Software Systems (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Library & Information Science (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
GB2308819.8A 2020-11-13 2021-11-13 Multiparametric discovery and optimization platform Withdrawn GB2617285A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063113571P 2020-11-13 2020-11-13
PCT/US2021/059275 WO2022104164A1 (en) 2020-11-13 2021-11-13 Multiparametric discovery and optimization platform

Publications (2)

Publication Number Publication Date
GB202308819D0 GB202308819D0 (en) 2023-07-26
GB2617285A true GB2617285A (en) 2023-10-04

Family

ID=81602639

Family Applications (1)

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GB2308819.8A Withdrawn GB2617285A (en) 2020-11-13 2021-11-13 Multiparametric discovery and optimization platform

Country Status (6)

Country Link
US (3) US11923045B2 (enExample)
EP (1) EP4244353A4 (enExample)
JP (1) JP2023549850A (enExample)
CN (1) CN116917474A (enExample)
GB (1) GB2617285A (enExample)
WO (1) WO2022104164A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022104164A1 (en) 2020-11-13 2022-05-19 Triplebar Bio, Inc. Multiparametric discovery and optimization platform
CN116047038B (zh) * 2022-12-21 2025-04-25 南雄市沃太化工有限公司 一种树脂固化条件的实验方法、装置、存储介质及设备
WO2025184561A1 (en) * 2024-03-01 2025-09-04 Deepmind Technologies Limited Optimizing molecule fitness using multiple diverse molecule design techniques
CN119580852B (zh) * 2024-11-19 2025-09-26 中国热带农业科学院环境与植物保护研究所 一种宏基因组数据集的生物地球化学元素循环分析方法及系统

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090130718A1 (en) * 1999-02-04 2009-05-21 Diversa Corporation Gene site saturation mutagenesis
US20100100331A1 (en) * 2003-08-01 2010-04-22 Dna Twopointo Inc. Systems and methods for biopolymer engineering
US20140256557A1 (en) * 2008-02-12 2014-09-11 Codexis, Inc. Method of generating an optimized, diverse population of variants
US20160210403A1 (en) * 2015-01-18 2016-07-21 The Regents Of The University Of California Method and system for determining cancer status
WO2019006022A1 (en) * 2017-06-27 2019-01-03 The Broad Institute, Inc. SYSTEMS AND METHODS FOR PREDICTING MHC CLASS II EPITOPE

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010111231A1 (en) * 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation of microfluidic droplets
EP3289362B1 (en) 2015-04-30 2022-04-13 European Molecular Biology Laboratory Microfluidic droplet detection and sorting
KR20190090081A (ko) 2015-12-07 2019-07-31 지머젠 인코포레이티드 Htp 게놈 공학 플랫폼에 의한 미생물 균주 개량
CA3033398A1 (en) 2016-08-22 2018-03-01 Sci-Bots Inc. Multiplexed droplet actuation and sensing in digital microfluidics
WO2018226900A2 (en) * 2017-06-06 2018-12-13 Zymergen Inc. A htp genomic engineering platform for improving fungal strains
US20190345566A1 (en) 2017-07-12 2019-11-14 The General Hospital Corporation Cancer polygenic risk score
WO2019178427A1 (en) * 2018-03-14 2019-09-19 Arbor Biotechnologies, Inc. Novel crispr dna targeting enzymes and systems
US11028402B2 (en) * 2018-04-06 2021-06-08 Wisconsin Alumni Research Foundation Polypeptide and yeast cell compositions and methods of using the same
JP7282809B2 (ja) * 2019-01-18 2023-05-29 富士フイルム株式会社 細胞生成支援装置、方法、及びプログラム
EP4004200A4 (en) 2019-09-13 2023-08-02 The University of Chicago METHOD AND APPARATUS USING MACHINE LEARNING FOR EVOLUTIONARY DATA-DRIVEN DESIGN OF PROTEINS AND OTHER SEQUENCE-DEFINED BIOMOLECULES
WO2022104164A1 (en) 2020-11-13 2022-05-19 Triplebar Bio, Inc. Multiparametric discovery and optimization platform

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090130718A1 (en) * 1999-02-04 2009-05-21 Diversa Corporation Gene site saturation mutagenesis
US20100100331A1 (en) * 2003-08-01 2010-04-22 Dna Twopointo Inc. Systems and methods for biopolymer engineering
US20140256557A1 (en) * 2008-02-12 2014-09-11 Codexis, Inc. Method of generating an optimized, diverse population of variants
US20160210403A1 (en) * 2015-01-18 2016-07-21 The Regents Of The University Of California Method and system for determining cancer status
WO2019006022A1 (en) * 2017-06-27 2019-01-03 The Broad Institute, Inc. SYSTEMS AND METHODS FOR PREDICTING MHC CLASS II EPITOPE

Also Published As

Publication number Publication date
CN116917474A (zh) 2023-10-20
EP4244353A4 (en) 2024-10-30
US20230174968A1 (en) 2023-06-08
WO2022104164A1 (en) 2022-05-19
US20230154562A1 (en) 2023-05-18
US20250061962A1 (en) 2025-02-20
US11972840B2 (en) 2024-04-30
GB202308819D0 (en) 2023-07-26
JP2023549850A (ja) 2023-11-29
EP4244353A1 (en) 2023-09-20
US11923045B2 (en) 2024-03-05

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