GB2613276A - Novel palatable pet food composition - Google Patents

Novel palatable pet food composition Download PDF

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GB2613276A
GB2613276A GB2302395.5A GB202302395A GB2613276A GB 2613276 A GB2613276 A GB 2613276A GB 202302395 A GB202302395 A GB 202302395A GB 2613276 A GB2613276 A GB 2613276A
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food composition
pet food
antioxidant
sodium
synthetic
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GB202302395D0 (en
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Kuerzinger Hubert
T Piering Scott
Kuhlmann Dietmar
Möller Ines
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Tetra GmbH
Spectrum Brands Inc
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Tetra GmbH
Spectrum Brands Inc
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Priority to GB2302395.5A priority Critical patent/GB2613276A/en
Priority claimed from GB2011234.8A external-priority patent/GB2583675B/en
Publication of GB202302395D0 publication Critical patent/GB202302395D0/en
Publication of GB2613276A publication Critical patent/GB2613276A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/111Aromatic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/105Aliphatic or alicyclic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/116Heterocyclic compounds
    • A23K20/121Heterocyclic compounds containing oxygen or sulfur as hetero atom
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • A23K20/147Polymeric derivatives, e.g. peptides or proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/158Fatty acids; Fats; Products containing oils or fats
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/163Sugars; Polysaccharides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/174Vitamins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/22Compounds of alkali metals
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/24Compounds of alkaline earth metals, e.g. magnesium
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/26Compounds containing phosphorus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Inorganic Chemistry (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Physiology (AREA)
  • Insects & Arthropods (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Birds (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)

Abstract

A pet food composition comprises at least one natural antioxidant, at least one synthetic antioxidant, and at least one acidity regulator to regulate the pH, wherein the pet food composition does not contain 1,2-dihydro-6-ethoxy-2,2,4-trimethylquinoline (ethoxyquin). The natural antioxidant may be one or more of tocopherol, ascorbic acid and a plant extract. The plant extract may be oregano extract, salvia extract and/or rosemary extract. Preferably the natural antioxidant is a combination of rosemary extract and tocopherol. The synthetic antioxidant is preferably a combination of butylhydroxytoluol and propyl-3,4,5-trihydroxybenzoate. The acidity regulator is preferably one or more of citric, formic, propanoic, lactic and sorbic acid. A use of the pet food composition for improving palatability of a pet food composition and/or for enhancing weight in a healthy pet is also claimed. A method of producing the pet food composition is also claimed, including the steps of mixing the natural and synthetic antioxidant(s) into a lipophilic matrix, mixing the pH regulator(s) into a premix, applying the lipophilic matrix and the premix to a solid matrix before obtaining the pet food composition, and obtaining the pet food composition. The pet food composition may be for a fish, reptile, bird or mammal, preferably a fish.

Description

NOVEL PALATABLE PET FOOD COMPOSITION
This application is being filed on 29 December 201' a PCT International patent application.
TECHNICAL FIELD OF TUE INVENTION
[1] The invention relates to a pet food composition comprising at least one natural antioxidant and at least one synthetic antioxidant and at least one acidity regutatol component to regulate the pH, wherein the pet food composition dies not contain 1 "2-d hyd ro-6-eth oxy-2,2,4-tri methyl qui nal ne. Further, the invention comprises a method of producing the pet food composition as well as the use of the pet food composition.
BACKGROUND ART
[2] An essential component of animal feeds is unsaturated fatty acids, e.g. n-3 and n-6 fatty acids Thus, animal feeds are in general susceptible to lipid oxidation, Lipid oxidation is initiated when a free radical (X, OFF or others) abstracts a hydrogen atom from polyunsaturated fatty acids (PUPA). A peroxyl radical is then formed due to the reaction of the PUFA radical with oxygen. This continues when the lipid peroxyl radical abstracts a hydrogen atom from a new PUPA (Hohner, 1993).
[3] To prolong the stability of the diet and the dietary components, stabilizing agents are often applied, which prevent the transmission of electrons of dietary components of the diet and thus obviate their spoilage. Those stabilizing agents act as electron donors and thus protect the highly reactive percentage of ingredients of the dietary components (such as unsaturated fats, yitamins, carotenoids etc.),, which are mainly essential nutrients for the target species. Further, the electron donors contribute to the security of lipid stability, to the prevention of toxic reaction products (peroxide) and they contribute to a long stability of the diet. Application of such electron donors is carried out in the dietary components such as fats and oils or directly into the diet. A highly effective electron donor is 1,2-dihydro-6-ethoxy22,4-trimethyl quint:dine (ethoxyquin).
[4] Especially spoiled lipids, which ha a vet taste and smell, often the target species refusing the diet. In a spoiled diet corrupted by disintegrated lipids, vitamins such as vitamin A and E are destroyed by aggressive atoms or molecules with at least one unpaired valence electrons. In the presence of breakdown products, pathological phenomena and toxic effects can also occur in the target species.
[5] In the course of the scientific re-evaluation in the Eli., concerns regarding ethoxyquin with respect to the safety of consumer and animals were raised. Thus, the aim was to provide an adequate substitute for ethoxyquin in view of pet food..
[6] in a study at the Bavarian State Institute for Agriculture, institute for 0 Fisheries (annual report 2004, experiments With rosemary extract as antioxidant in diets for rainbow trout) experiments with a natural antioxidant such as rosemary extract as an electron donor were tested in a diet for salmonids, in the experiment, it was found that rosemary extract as a natural antioxidant results in reduced food intake in comparison to the diet without the natural components. Thus, the prior art teaches that rosemary extract as a natural antioxidant alone is not sufficient to increase the food intake in the target species.
[7] Thus" there is a need in the art for other adequate components, preferably substituting ethoxyquin.
[8] Therefore, the objective of the invention is to comply with this need.
[009] The solution of the invention is described in the following, exemplified in the appended examples" illustrated in the figures and reflected in the claims.
SUMMARY OF THE INVENTION
[010] Surprisingly, it has now been found that the combination of one or more natural antioxidants and one or more synthetic antioxidants in combination with one or more additives to lower the pH value not only stabilizes and prevents lipid spoilage in the diet, but also increases the acceptance and attractiveness of the diet in the target species synergistically by an intensification and reversal of the individual [011] The invention comprises a pet food composition comprising least one natural antioxidant, and ii) at least one synthetic antioxidant, and iii) at least one acidity regulator component to regulate the pH, wherein the pet food composition does preferably not contain,2-dihydro-6-eihoxy-2,2,44ri methylquinoli ne.
[12] Further, the invention may envisage the pet fbod composition, wherein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or plant extract.
[13] The plant extract preferably comprises oregano, salvia and/or rosemary extract.
[14] Additionally, the invention may envisage the pet food composition, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol.
[15] The ratio of rosemary extract to tocopherol may be 1:0.1 to 15.
[016] The invention may encompass the pet food composition, wherein the at least one synthetic anti oxidant is selected from the group consisting of butylhydroxyanisol butyl hydroxvtol no!, propy1-3, 4 5-trihydroxyhenzoate, sulfur dioxide, sodium sulfite; sodium hydrogensulfite, sodium di sulfite, potassium disulfite, calcium sulfite, calcium hydrooensuifite, potassium hydrogen sulfite.
sodium trascorbate, calcium T.,-ascorbate, esters, octygatiate., butylhydroquinone (TBHQ), citric acid salts, sodium citrate, potassium calcium disodium EDTA, diphosphates, di sodium diphosphate, trisodi m diphosphate, tetrasodi an diphosphate, Di phosphate diphosphate, calcium diphosphate, triphosphates, pentasodium triphosphate, pentakalium triphosphaie, polyphosphate, sodium polyphosphate, potassium polyphosphate, sodium calcium polyphosphate; calcium polyphosphate; tin-It-chloride.
[017] The at least one synthetic antioxidant May be in the composition II an amount from 0.001% to 1.0% based on the crude fat content of the pet food composition.
[018] Further, the y comprise the pet food composition, wherein the at least one acidity regulator component is selected from the group consisting of citric acid, formic acid, propanoic acid, lactic acid and soitic acid.
[019] The at least one acidity regulator component may be in the composition in an amount from 0.001% to 10% by weight of the composition.
[020] The invention may envisage the pet food composition wherein the pH is below 6.2.
[021] The invention may comprise the pet food composition, wherein i) th one natural antioxidant is a combination of rosemary extract and tocophe ratio of:0,1 to 1:5, ii) the at least one synthetic antioxidant is butnyl tiydroxytoiuoi and/or propy1-3,4,54rihydroxybenzoate, and iii) the at feast one acidity regulator component is citric acid.
[22] The invention may comprise the pet: food composition, further comprising a dietary component selected from the group consisting of a fat an oil, a protein, a vitamin, a starch, a flavoring agent and a coloring agent.
[23] The at least one natural antioxidant and the at least one synthetic antioxidant may be integrated homogeneously into a lipophilic matrix.
[24] The invention envisages a method of producing a pet food composition, 0 method comprising a) mixing the additives of a pet food composition comprising i) at least one natural antioxidant, and ii) at least one synthetic antioxidant into a lipophilic matrix, b.) mixing the at least one acidity regulator component to regulate the pH into a premix, c) applying the enriched lipophilic matrix of step a) and the enriched premix of step b) to a solid matrix before obtaining the pet food composition, d) obtaining the pet food composition.
[25] The invention thither encompasses the use of a pet food composition for improving the palatability of the pet food composition and tr for enhancing the weight in a healthy pet.
BRIEF DESCRIPTION OF THE FIGURES
[26] Fig. I: Weight increase (9/9 / pisces) of aquatic subjects, The synergistic effect of the combination of synthetic and natural antioxidants in a specific ratio with the effect of a pH-lowering and thus acceptability-improving component (diet SNC), increases the weight of aquatic subjects (fish, e.g. Osteichthyes such as red phantom tetra and yellow cichlid) by up to 14% to the control diet without supplementation. The addition of natural antioxidants (diet NC) adversely affects the weight of the individual species by up to 6% compared to the control diet.
DETAILED DESCRIPTION OF THE INVENTION
[27] The invention provides a pet food composition comprising i) at least one natural antioxidant, and ii) at least one synthetic antioxidant, and iii) at least one acidity regulator component to regulate the p1-i. Preferably, wherein the pet food composition does not contain 1,2-dihydro-6-ethoxy-2,2,4-trimethylquinoline.
[28] The term "pet food compositi OTC refers to a wet or dry food composition for pets, in particular for fish. It may be in the form of a moist food, semi-moist food, dry food, supplement or treat. The pet food composition may be in kibble form, flakes:, pellets, sticks, granules, gelfood or tablets and the like. Preferably, the pet food composition is dry food. Preferably, the pet food composition is for fish.
[29] The term "natural" means naturally occurring or occurring in the nature. Thus, natural antioxidants occur in the nature. Once the natural antioxidant is identified, it may' be isolated from the nature or by biological and/or chemical means. Yet, if isolated by biological and/or chemical means, it does not change the origin of the natural antioxidant. which is natural.
[30] As used herein, the term "antioxidant" is a substance that, wrr s it in a mixture containing an oxidizable substrate or biological molecule, significantly delays or prevents oxidation of the substrate biological molecule. Antioxidants can act by scavenging biologically important reactive free radicals or other reactive oxygen species (02 1:11202, .0H, HOD, fetryl" peroxyl, peroxynitrite, and al koxyl), or by preventing their formation, or by catalytically converting the free radical or other reactive oxygen species to a less reactive species. Relative antioxidant activity can be measured by cyclic voltammetry studies, where the voltage (x-axis) is an index of relative antioxidant activity. The voltage at which the lirst peak occurs is an indication of the voltage at which an electron is donated, which in turn is an index of antioxidant activity. An antioxidant may also he called (feed) additive, which is added to the pet food composition.
[31] The at least one natural antioxidant may be selected from the group consisting of tocopherol, ascorbic acid, plant extract, and any combination thereof [32] The at least one natural antioxidant may be selected from the group consisting of tocopherol(s) and/or ascorbic acid and/or plant extract(s). The at least one natural antioxidant may be tocepherol(s) and ascorbic acid and plant extract(s). The at least one natural antioxidant may be tocopherol(s) and ascorbic acid. The at [east one naniral antioxidant may be tocopherol(s) and plant extract(s). The at least one natural antioxidant may be tocopherol(s) or ascorbic acid or plant extract(s).
[33] Tocopherols are a class of organic chemical compounds having vitamin E activity. The class consists of mainly, and 6, but also a-tocopherols, wherein each form has a different biological activity on and ingold 981. .1 Am, Sac 103: (3472-6477). As a food additive, tocopherol is labelled with these E. numbers: E306 (tocopherol), E307 (a-tocopherol), E308 (y-tocopherol), and E309 (8-tocopherol). These are all approved in the USA, EU and Australia and New Zealand for use as antioxidants. In the invention a mixture of tocopherols may be 0 used. in particular, or, 0-, 7-, and 6 tocopherols may be used, wherein the mixture may comprise > 50% non a-tocopherois.
[34] The term "ascorbic acid (Coli506)" is an organic compound derived from a monosaccharide. It is highly soluble in water. Ascorbic acid refers to vitamin C and is used as a dietary supplement. In food industry it has been exploited for its antioxidant and stabilizing ability.
[35] The term "plant extract" refers to a substance made by extracting a part of a raw plant. material. Extraction may be achieved by using a solvent such as ethanol or water. Plant extracts may he used. as tinctures or in powder form. In the invention (the) plant extract(s) may be used as a tincture or as a powder form [036] Preferably, the plant extract comprises oregano, salvia and/or rosemary extract. The plant extract may comprise oregano extract and salvia extract and rosemary extract The plant extract may comprise oregano extract or salvia. extract or rosemary extract. The plant extract is oregano extract, preferably the plant extract is salvia extract and more preferably the plant extract is rosemary extract.
[037] The inventors found that the at least one natural antioxidant is preferably a combination of rosemary extract and tocopherol(s).
[038] The ratio of rosemary extract to tocopherol may be 1:0.1 to 1 to 1:5. Preferably, in the mixture the ratio of rosemary extract to tocopherol is 1:0 4 to 1.3, more preferably the ratio of rosemary extract to tocopherol is 1:1 to 1:1.3 [039] The dosage of the individual component (e.g., rosemary extract or tocopherol including carrier) or of the mixture (e.g., rosemary extract and toconhetol including carrier) is from 10 to 400 mg/kg diet, preferably from 20 to 200 mg/kg diet, more preferably from 70 to 95 mg,/kg diet, such as 70, 71, 72, 73, 74, 75, 76; 77, 78, 79, 80, 81, 82" 83, 84, 85" 86; 87, 89, 90" 91, 92, 93, 94" or 95 ing/kg diet If a mixture may be. used, the ratio of the components (rosemary extract and tocopherOl and carrier) to achieve a specific dosage is variable depending on the dosage of interest. in this context; the term "carrier" refers to a diluent, adjuvant, or vehicle with which the components may be administered. Carriers may be sterile liquids, such as water and oils including those of petroleum, animal, vegetable or synthetic origin, such as peanut oil, soybean oil, mineral oil, sesame oil and the like. Here; the carrier (e.g. water) and the natural antioxidants such as rosemary extract and tocopherol may form a solution.
0 [040] The term means not naturally occurring. rather of pertaining to, proceeding by or involving,synthesis Synthetic antioxidants pertain to compounds/substances formed through a chemical process such as synthesis by human agency, as opposed to those of natural origin.
[041] The at least one synthetic antioxidant may be selected from the group consisting of butylnydroxyanisol, buthylhydroxytoltiol, propy1-3; 4, 5-trihydroxybenzoate, sulfur dioxide, sodium sulfite, sodium hydrogensulfite; sodium disulfite, potassium disulfite, calcium sulfite, calcium hydrogensulfite, potassium hydrogen sulfite, sodium 1-ascorbate, calcium L-ascorbate; esters, octygallate; tertiary butylhydrocntimine (TI3HQ)" citric acid salts, sodium citrate, potassium citrate, calcium disodium EDT& diphosphates, disodium diphosphate, trisodium diphosphate, tetrasodi urn di phosphate, Diphosphate di phosphate, calcium di phosphate, triphosphates, pentasodium triphosphate, pentakalium triphosphate; polyphosphate, sodium polyphosphate, potassium polyphosphate, sodium calcium polyphosphate, calcium polyphosphate" tin-lhchloride, preferably the at least one synthetic antioxidant is buthylhydroxytoluoi and/or propy1-3, 4, 5trihydroxybenzoate. The at least one synthetic antioxidant may be buthyl hydroxy tin! and propyi droxybenzoate. The at least one synthetic antioxidant may be buthyihvdroxytoiuol or propy1-3, 4, 5-trihydroxybenzoate. Also comprised by the invention may be the at least one synthetic antioxidant being butyl hydroxy ani sol and/or propyl -3, 4, fdroxrnenzoate. Thus; the at least one synthetic antioxidant may be butyihydroxyanisol and propy1-3, 4, 5,-trihydroxybenzoate The at least one synthetic antiOxidant may be butylhydroxyani sol or propyl -3; 4, 5,-trihydroxybenzoate.
[42] The term "hi froxytoluol (Billy refers to an antioxidant and is a lipoplailic organic compound, chemically a derivative of phenol. it is also called "dibutvihydroxytoluene. In the United States, it is classified as generally recognized as safe (GRAS) based on a National Cancer institufe study from 1979. In the Euopean Union it is primarily used as an antioxidant food additive having the E. number E321.
[43] The term "bu 1.oxyanisol" refers to is an antioxidant consisting of a mixture of two isorneric organic compounds, namely 2-tert-buty1-4-hydroxyanisole 0 and 3-tert4buty1-44iydroxyanisole. It is a waxy solid and used as a food additive having the E number E320 [44] The term "propy4-3, 4, 5-trihydroxybenzoate" refers to propyl gallate and is an ester formed by the condensation of gallic, acid and propanol. Propyi gallate acts as an antioxidant and is added to foods, which contain fats and oils, to prevent oxidation. As a food additive, it is used under the E number fil310.
[45] The at least one synthetic antioxidant may be in the composition in an amount from 0.001% to 1.0% based on the crude fat content of the pet food composition. Preferably from 0.01 to 0.04% based on the crude fat content of the pet food composition, more preferably from 0,015 to 0.025% based on the crude fat content of the pet food composition, such as 0.015%, 0.016%, 0.017%, 0.018%, 0.019%, 0.020%, 0.021%, 0.072%, 0.023%, 0,024%, 0.025% based on the crude fat content Of the pet food composition. AS an example for a pet food composition with the at least OM synthetic antioxidant in an amount of 0.02% based on the crude fat content of the pet food composition, 10 mg/ kg synthetic antioxidant may be used for a pet food composition with 5% crude fat and 30 mg / kg synthetic antioxidant may be used for a. pet food composition with 15% crude fat. The dosages (in an amount from 0.001% to 1.0%, or in an amount from 0.01 to 0.04%, preferably in an amount from 0.015 to 0.025%) may apply to a single synthetic antioxidant (e.g., buthythydroxytoluol or propyl--3, 4, 5-trihydroxybenzoate) or it may apply to a mixture of synthetic antioxidants (e.g., such as buthylhydroxytcluol and propy1:3, 4, 5-trihydrOxyberizoate). In this context, the Mixture of the synthetic antioxidants May be based on the sum of the synthetic antioxidants.
[46] 'The term "erode fat content of the pet food composition" is determined genera. animal fecci analysis such as the classical icier mittelanalyse" as it is known to a person skilled in the art. The classical "W Futtermittelanalyse" comprises standard processes, being necessary to determine nutritional value of the animal diet. Here, it is differentiated between crude ash which refers to anorganic substances and organic substances e.g., crude fibers (such as cellulose, pentosans and the like), crude proteins (such as peptides, amino acids and the like), crude fat (triglyceride, wax, carotene, oils and the like) and nitrogen-free substances.
[47] The term "acidity regulator component" refers to a component which 0 regulates the pH. In other words the component is a pH-lowering and thus acceptability-improving component. The at least one acidity regulator component may be used as an acceptance and attractiveness enhancer and weight enhancer. An acidity regulator component may also be called (feed) additive.
[48] The at least one acidity regulator component may be selected from the group consisting of citric acid, formic acid, propanoic acid, lactic acid and sorbic acid. The at least one acidity regulator component may be citric acid and formic acid and anoic acid and lactic acid and sorbic acid. The at least one acidity regulator orient may be citric acid or formic acid or propanoic acid or lactic acid or sorbic acid. The at least one component may be citric acid and formic acid; or citric acid and propanoic acid; or citric acid and lactic acid; or citric acid and sorbic acid.
Preferably, the at least on.e acidity regulator component is formic preferably the at least one acidity regulator component is lactic acid and most preferably the at least one acidity regulator component is citric acid.
[49] The term "citric acid (C6EW/7.y is a weak organic tricarboxylic. It is an intermediate in the citric acid cycle or aerobic organism. It naturally occurs in Glans fruits. Citric acids and other acids such as formic, propanoic, lactic and sorbie acid salts and mixtures are usually added into the diets as preservatives in a very low dosage of about 0.005% to prevent microbiological spoilage. These preservatives also find in the lowest concentrations of from 0.0005 to 0.005% in commercial products for the prevention of the spoilage of lipids usc [50] The at least One acidity regulator component may he in the composition in an amount from 0.001% to 10% by weight of the composition. Preferably, the dosage of the component for lowering the pH value is in an amount from 0.01% to 1% by of the pet food composition and more preferably from 0.028% to 0.034% by weight of the pet food composition, such as 0.028%, 0,029%, 0,030%, 0.031%, 0.032%, 0.033% and 0.034% by weight of the pet food composition, in order to lower the pH of the pet food composition properly and to achieve an increased acceptance of the food in the target species, the amount (dosage) of the acidity regulator component may be higher than 0.005%, the usual dosage of the preservatives such as citric acid and other acids as for example formic acid, propanoic acid, lactic acid and sorbic acid as well as their salts and mixtures In this context, the term 'by weight", "percentage by weight" or (14)" refers to the 0 weight of the final pet food composition [051] During the application of the combination of at least one natural and at least one synthetic antioxidant, the synthetic and natural antioxidants need to be used in a very specific ratio in order to achieve the synergistic effect in combination with at least one acidity regulator component as stabilizing agents in the pet food composition. The ratio of synthetic (see [45]) vs natural antioxidants ([38]) in combination with additives (acidity regulator components) such as e.g. cit ) may vary depending on the lipid content of the pet food composition.
[M] Further, the pH of the pet food composition may be below 6.2. The term 'belowi' means below or equal. Preferably, the pH is 6.2 or belosy 6.2, such as 6.1, 6.0, 5.9, 5.8, 5.7 5.5, 5.4, 5.3, 5.2, 5.1, 5.0, 4.9; 4.8, 4.7, 4.6, 4.5, 4.4, 4.3, 4.2; 4.1, 4.0, 3 0 3 5.3.4, 3.3, 3.2, 3.1, 3.0.
[53] The invention further comprises a pet food composition comprising, at least one natural antioxidant, wherein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid andlor plant extract and ii) at least one synthetic antioxidant, and iii) at least one acidity regulator component to regulate the pH, wherein the pet food composition does preferably not contain 1,2-dihydro--6-ethoxy-2,2" 4-trim ethyl quinoli ne.
[54] Further; the invention comprises a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is selected from the group of tocopherol andlor ascorbic acid and/or plant extract, wherein the plain extract comprises oregano, salvia and/or rosemary extract and ii) at least one synthetic antioxidant, and Iii) at least one acidity regulator component to regulate the herein the pet food composition does preferably not contain i,2dihydro ethoxy-2,2,44rimethylquinoline_ [55] Additionally, the invention comprises a pet food composition comprising ij at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocepherol and ii) at least one synthetic antioxidant, and iii) at least one acidity regulator component to regulate the pH, y'herein the pet food composition does' preferably not contain 1,2-dihydro-6-ethoxy-m ethyl qui nol in e.
[56] The invention encompasses a pet food composition comprising j at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol in a ratio of 1:0.1 to 1:5 and ii) at least one synthetic antioxidant, and iii) at least one acidity regulator component to regulate the p wherein the pet food composition does preferably not contain 1"2-diliydro-6-ethoay-2,2,44rimethylquinoline. 3_5
[57] Additionally, the invention encompasses a pet food composition comprising 1) at least one natural antioxidant, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is selected. from the group consisting of butylhydroxyanisol, buthylhydroxytoluol, propy1-3, 4, 5-itihvdroxybenzc,,ate, sulfur dioxide, sodium sulfite, sodium hydrogensuifite, sodium disuifite, potassium disulfite, calcium sulfite, calcium hydrogensulfite, potassium hydrogen. sulfite, sodium L-ascorbate, calcium L-ascorhate, esters, octygal late, tertiary butylhydroquinone (THEN),), citric acid salts, sodium citrate, potassium citrate, calcium disodium EDTA., &phosphates., disodium diphosphate" trisodium diphosphate, tetrasodium diphosphate. Diphosphate diphosphate, calcium diphosphate, triphosphates, pentasodium tnphosphate. pentakalium triphosphate, polyphosphate, sodium polyphosphate, potassium polyphosphate, sodium calcium polyphosphate, Calthilri polyphosphate:, tin-H-chloride, and iii) at least one acidity regulator component to regulate the pH, wherein the pet food composition does preferably not contain 1,2-dithydro-6-etboxy-2,2,74-Mmethylquinoline [58] The invention further comprises a pet food composition comprising i) at least one natural antioxidant, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is in the composition in an amount from 0,001% to 1.0% Iii-based on the crude fat content of the pet food composition and iii) at least one acidity regulator component to regulate the pH, wherein the pet food composition does preferably not contain, 2-di hy dro-6-erhoxy-2,2,4-tri me thy lquinoli ne [059] The invention further comprises a pet food composition comprising i at least one natural antioxidant, and ii) at least one synthetic amioxidant, wherein the at least one synthetic anti oxidant is buthyl hydroxytoluol and/or worry -3, 4,, .5-Mihydroxybenzoate and iii) at least one acidity regulator component to regulate the pH, wherein the pet food composition does preferably not contain 1,2-dihydro-6-ethoxy-2,2,4-trimethylquinoline.
to [060] The invention further comprises a pet food composition comprising 1) at least one natural antioxidant, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is buthyl hy droxy an i sol and/or props:1-3, 4, 5-trihydroxybenzoate and iii) at least one acidity regulator component to regulate the pH, wherein the pet food composition does preferably not contain 1,2-dihydro-6-ethoxy-2,2,4-Mimethylquinoline.
[61] The inventnn envisages et food composition cam at least one natural antioxidant, wherein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a plant extract, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is selected from the group consisting of buthylhydroxyanisol, buthylhydroxytoluol, propy1-3, 4, 5-trihydroxybenzoate, sulfur dioxide, sodium sulfite, sodium hydrogensulfite, sodium di sul fite, potassium di su fi te, calcium sulfite, calcium hydrogensulfite, potassium hydrogen sulfite, sodium L-ascorbate, calcium L-ascorbato, esters, octygallate, tertiary butylhydroquinone (TBHQ), citric acid salts, sodium citrate, potassium citrate, calcium di sodium EDTA, diphOsphates, di sodium diphosphate, tri sodium diphosphate, tetrasodititn diphosphate, Di phosphate diphosphate, calcium diphosphate, triphosphates, pentasodium triphosphate, pentakalium triphosphate, polyphosphate, sodium polyphosphate, potassium polyphosphate, sodium calcium polyphosphate, calcium polyphosphate, tin-11-chloride, and iii) at least one acidity regulator Component to regulate the pH, wherein the pet food composition does preferably not contain,2-dihydro-6-eth oxy-2,2,4-trimethyl qui nol ine.
[62] The invention envisages a pet food composition comprising, i) at least one natural antioxidant, wherein the at least one natural antioxidant elected from the group of tocopherol and/or ascorbic acid and/or a. plant extract, wherein the plant extract comprises oregano, salvia and/or rosemary extract and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is selected from the group consisting of buthythydroxyanisol, buthythydroxytoluol, propy1-3, 4, 5-trihydroxyberizixae, sulfur dioxide, sodium sulfite, sodium hydrogens-unite, sodium &sulfite, potassium disunite, calcium sulfite; calcium hydrogensullite, potassium hydrogen sulfite, sodium L-ascorbate, calcium L-ascothate, esters, octygallate, tertiary hutylhydroquinone (T131H0), citric acid salts, sodium citrate, potassium 0 citrate, calcium &sodium EDTA, &phosphates, &sodium &phosphate, nisodium &phosphate, totrasodium di phosphate, Diphosphate di phosphate, calcium diphosphate" uiphosphates, pentasodium triphosphate" pentakahurn triphosphate, polyphosphate, sodium polyphosphate, potassium polyphosphate; sodium calcium polyphosphate, calcium polyphosphate; tin-II-chloride, and iii) at least one acidity regulator component to regulate the pH, wherein the pet food composition does preferably not contain 1,2-dihydro-61-ethoxy-2,2,4-trimethylquinotine.
[63] The invention envisages a. pet food composition comprising at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is selected from the group consisting of buthylhydroxivanisol, buthylhydroxytoluol, propy1-3; 4, 54rihydroxyhenzoate, sulfur dioxide, sodium sulfite, sodium:hydrogensulfite, sodium disunite, potassium &sulfite, calcium sulfite, calcium hydrogensuitite, potassium hydrogen sulfite, sodium L-ascorbate, calcium L-a.scorbate, esters; octygallate, tertiary butythydroquinone (TBHQ), citric acid salts, sodium citrate,potassium citrate, calcium di sodi um EDTA, di ph osphates, di so& uin diphosphaLe, trisodium diphospbate, tetrasodium diphosphate; Diphosphate diphosphate, calcium diphosphate, triphosphates, peutasodurin triphosphate; pentakalium triphosphate, polyphosphate, sodium polyphosphate, potassium polyphosphate, sodium calcium polyphosphate, calcium polyphosphate, tin-11-chloride, and iii) at least one acidity regulator component to regulate the pH, wherein the pet food composition does preferably not contain 1,2-dihydro-6-ethoxv-2,2,4-trimethylquinoline, [64] The invention encompasses a pet food composition comprising 1) at least one natural antioxidant, wherein the at least one natural antioxidant is a cot don of rosemary extract and tocopherol in a ratio of:0.1 to 1:5, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is selected from the group consisting of buthylhydroxyanisol, buthylhydroxytolual, propy1-3, 4, 5-trihydroxybenzoate, sulfur dioxide, sodium sulfite, sodium hydrogensulfite, sodium disulfite, potassium disulfite, calcium sulfite, calcium hydrogensulfite, potassium hydrogen sulfite, sodium L-a.scorhate, calcium L-aseorbate, esters, octygallate, tertiary burylhydroquinone (17:13HQ), citric acid salts, sodium citrate, potassium citrate, calcium chsodium EDTA, ciphosphates, disodium diphosphate, trisodium 0 diphosphate, tetrasodiuth diphosphate. Di phosphate diphosphate. calcium diphosphate, triphosphates, pentasodium triphosphate, pentakalium triphosphate, polyphosphate, sodium polyphosphate, potassium polyphosphate. sodium calcium polyphosphate, calcium polyphosphate, tin41-chloride. and iii) at least one acidity regulator component to regulate the pH, wherein the pet food composition does preferably not contain 1, fdro-6-ethoxy-2"2,4-trimethyliminoline, [065] The itiventi envisages a. pet food composition comprising 0 at least one the at least one natural antioxidant is selected from the natural antioxidant, group of tocopherol and/or ascorbic acid and/or a plant extract, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is in the composition in an amount from 0.001% to 1.0% based On the crude fat content of the pet food composition, and iii) at least one acidity regulator component to regulate the pti, wherein the pet food composition does preferably not contain 1,2-dihydro-6-ethoxy-2,2,4-trim [066] The invention comprises a pet food composition cothpris.ng i) at least one natural antioxidant, wherein the At least one natural antioxidant is selected from the group of tocopherol and/or ascorbic and/or a plant extract, wherein the plant extract comprises oregano, salvia and rosemary extract and ii) at least one synthetic, antioxidant, wherein the at least one synthetic antioxidant is in the composition in an antount from 0.001% to 1.0% based on the crude [hi content of the pet food composition, and iii) at least one acidity regulator component to regulate the pH, wherein the pet food composition does preferably not contain 1,2-hydro-6-ethoNy-2,2,4-trimethylquinoline.
[67] inc,et food composition corn.g i) at least one natural antioxidant; wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is in the composition in an amount from 0.001% to 1.0% based on the crude fat content of the pet food composition; and at least one acidity regulator component to regulate the pH, wherein the pet food composition does preferably not contain 1,2-dihydro-*6--ethoxy-2,2,4-En ethylquitioline.
[68] The invention encompasses a pet food composition comprising i) at least one 0 natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol in a ratio of 1:0.1 to 1:5 and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is in the composition in an amount from 0.001% to 1.0% based on the crude tht content of the pet food composition, and iii) at least one acidity regulator component to is regulate the pH, wherein the pet food composition does preferably not contain 1,2-di hydro-6-ethoxy-2,2,44ri methyl qui n [69] The invention envisages a. pet food composition composing i) at least one natural antioxidant, wherein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a plant extract., and ii) at least one synthetic antioxidant, wherein at least one synthetic antioxidant is buthythydroxytothol and/or propy1-13, 4. 5-trihydroxybenzoate, and iii) at least one acidity regulator component to regulate the pH, wherein the pet food composition does preferably not contain 1,2-dihydro-45-ethoxy--2,2,4-1rimethylquinoline.
[70] The invention comprises a pet food composition comprising i) at least one natural antioxidant; wherein the at least one natural antioxidant is selected from the group of tocopherol arid/or ascorbic acid and/or a. plant extract, wherein the plant extract comprises oregano, salvia andlor rosemary extract and ii) at least one synthetic antioxidant, wherein. least one synthetic antioxidant is bothylhydroxytothol arid/or trihydroxybenzoate, and iii) at least one acidity regulator component to regulate the pH, wherein the pet food composition does preferably not contain 1,2-dihydro-6-ethoxy-2,2,41-trimethylquiholine.
[71] The invention comprises a pet food composition comprising i) at least one natural antioxidant; wherein the at least one natural antioxidant is a combination of rosemary exiract and tocopherol, and U.) at least one synthetic antioxidant" wherein the at least one synthetic antioxidant is huthylhydroxytoluol and/or propy1-3; 4; 5-trihydroxybenzoate, and iii) at least one acidity regulator component to regulate the pH, wherein the pet food composition does preferably not contain 1,2-dihydro-6-ethoxy-2,2,4-trimethylquineline.
[72] The invention comprises a pet food composition comprising i) at natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol in a ratio of 1:0.1 to 1:5, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is buthvIhydroxytoluol and/or propyI-3, 4, 54Uifiydroxybenz.oate, and iii) at least one acidity regulator component to regulate the pH, wherein the pet food composition does preferably not contain 1,2-dihydro-6-ethoxy-2,2,4-trimethylquinoline.
[73] The invention envisages a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a plant extract, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is buthylhydroxyanisol and/or propy1-3, 4, 54rihydroxyberizoate, and iii) at least one acidity regulator component to regulate the pH, wherein the pet food composition does preferably not contain 1,2-dihydro-6-ethoxy-2; imethyiquinoline.
(074] The invention comprises a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a plant extract, wherein the plant extract comprises oregano, :salvia and/or rosemary extract and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is buthylhydroxyanisol and/or propy1-3, 4, 5-trihydroxybenzoate, and iii) at least one acidity regulator component to regulate the pH, wherein the pet food composition does preferably not contain 122-dilrydro-6-ethoxy-2,2,44rimethylquinoline [075] The invention comprises a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is buthvihydroxyanisol and/or propyl-3, 4, 5-trihydroxybenzoate, and iii) at least one acidity regulator component to regulate the herein the peL food composition does preferably not contain i,2dihydro ethoxy-22,44rimethylqictinoline.
[076] The invention comprises a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is a combination Of rosemary extract and tocopherol in a ratio of 1;0.1 to 1:5, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is buthylhydroxyanisol and/or propv1-3, 4, 5-trihydroxyberizoate; and east one acidity regulator component to regulate the pH, wherein the pet food composition does preferably not contain 1,2-dihydro-6-ethoxv-2,2,4-trimethy1quinoline [0771 The invention envisages a pet food composition comprising 1) at least one natural antioxidant, and ii) at least one synthetic antioxidant, and iii) at least one acidity regulator component to regulate the lll, wherein the at least one acidity regulator component is:selected front the group consisting of citric acid, formic acid; propanoic acid, lactic acid and sorbic acid, wherein the pet food composition does preferably not contain 1,2-dihydro-6-ethoxy-2,2,4-trimethylquinoline [78] The invention envisages a. pet food composition comprising i) at least one natural antioxidant; and ii) at least one synthetic antioxidant, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is in the composition in an amount from 0.001% to 1094, wherein the pet food composition does preferably not contain 1,2-dihydro-6-ethoxy2,2,4-trimethyleuinoiine.
[79] The invention envisages a pet food composition comprising i) at least one natural antioxidant, and ii) at least one synthetic antioxidant, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is citric acid, wherein the pet food composition does preferably not contain 1,21-dihydro-biettioxv-2,2,4-trimethylquinoline.
[80] The invention envisages t food composition comp. rig 1) at least one natural antioxidant; wherein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a plant extract, and ii) at least one synthetic antioxidant, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is selected from the group con s s ti ng c acid" formic acid, propanoic acict, lactic acid and sortie acid, wherein the pet food composition does preferably not contain 1,2-idihydro-6-ethoxy2,2,4-trimethyl ui noi in e [81] The invention envisages a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a plant extract, wherein the plant extract comprises oregano, saivia andlcir rosemary extract and ii) at least one synthetic antioxidant, and iii) at least one acidity regulator component to regulate the wherein the at least one acidity regulator component is selected front the group 0 consisting of citric acid, formic acid, propanoic acid, lactic acid and sorbic acid, wherein the pet food composition does preferably not contain 1,2-dihydro--6-ethoxy2,24-trimethylquinoine.
[82] The invention envisages a pet food composition comprising i at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol, and ii) at least one synthetic antioxidant, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is selected from the group consisting of citric acid, formic acid, propanoic acid., lactic acid and sorbic acid, wherein the pet food composition does preferably not contain 1,2-di hydro-6-ethoxv-2,2,4-trimethylquinoline.
[83] The invention encompasses a pet food composition comprising if at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol in a ratio of 1:0.1 to 1:5, and ii) at least one synthetic antioxidant, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is selected from the group consisting of CitEiC acid, formic acid, propanoic add, lactic acid and sorbie acid, wherein the pet food. composition does preferably not contain 1,2-dihydro-6-ethoxv--ro ethyl quinoli ne.
[084] The invention envisages a pet food composition compris at least one natural antioxidant, wherein the At least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a plant extract, and ii) at least one synthetic antioxidant, and iii) at least one acidity regulator component to regulate the phi., when. the at least one acidtty regulatcir component 3 in the composition in an amount from 0.001% to 10% wherein the pet food composition does preferably not contain 1,2-dihydro-6-ethoxy-2,2,4-trimethylquinoline.
LOBS] The invention envisages a pet food composition composing i) at least one natural antioxidant, wherein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a plant extract, wherein the plant extract comprises oregano, saivia andlor rosemary extract and ii) at least one synthetic antioxidant, and iii) at least one acidity regulator component to regulate the wherein the at least one acidity regulator component is in the composition in an amount from 0:001% to 10%, wherein the pet food composition does preferably not contain 1,2-dihydro-6-ethoxy-2,2,4-trimethylquinoline.
[86] The invention envisages a pet food composition corm ing at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol, and at least one synthetic antioxidant; and ill) at least one acidity regulator component to regulate the pH" wherein the at least one acidity regulator component is in the composition in an amount from 0.001% to 10%, wherein the pet food composition does preferably not contain hydro-o-othoxy-2,2,4-bifilethylquinoline.
[87] The invention encompasses a pet food composition comprising at least one natural antioxidant; wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol in a ratio of 1:0.1 to 1:5, and ii) at least one synthetic antioxidant, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is in the composition in an amount from 0 001% to 10%, wherein the pet food composition does preferably not contain 1,2-dihydro,-6-ethoxy-2,2,4-trimethy1quinoline.
[88] The invention envisages a pet food composition comprising at least one natural antioxidant, wherein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a. plant extract, and ii) at least one synthetic antioxidant, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is citric acid, wherein the pet food composition does preferably not contain 1,2-dihydro-6-ethoxy-2,2,4-tri methyl qui nol ne [89] The invention envlsages a pet food composition comprising i) at least one natural antioxidant; wherein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a plant extract, wherein the plant extract comprises oregano, salvia and/or rosemary extract and it) at least one hetic antioxidant, and iii) at least one acidity regulator component to retgulate the -em n the at least one acidity regulator component is citric acid, wherein the pet food composition does preferably not contain 1,2-dihydrorti-ethoxv ethylquinoline.
[90] The invention envisages a pet food composition comprising it at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol, and ii) at least one synthetic antioxidant, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is citric acid, wherein the pet food composition does preferably not contain 1,12-dihydro-6-ethoxy-2,24-trimethylquinoline.
[091] The invention encompasses a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol in a ratio of 1:0.1 to 1:5, and at least one hetic antioxidant, and iii) at least one acidity regulator component to retgulate the wherein the at least one acidity regulator component is citric acid, wherein the pet food composition does preferably not contain 1,2-dihydro-6-ethoxy-2,2,4-ti -dayiquinoline.
[092] The invention envisages a pet food composition comprising I) at least one natural antioxidant, wherein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a plant extract, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is selected from the group consisting of buthyihydroxyanisol, buthylhydroxytelubl, propy1-3; 4, 5-trihydroxybenzoate, sulfur dioxide, sodium sulfite, sodium liydrogensuifite, sodium disulfite, potassium disuifite, calcium sulfite; calcium hydrogensultite, potassium hydrogen sulfite, sodium Liascorhate, calcium L-ascorbate, esters, octygallate, tertiary butylhydroquinone (111314Q), citric acid salts, sodium citrate, potassium citrate, calcium disodium EDTA, diphosphates, disodium dinhosphate, trisodium diphosphate, tetrasodium diphosphate. Diphosphate diphosphate, calcium diphosphate, triphosphates" pentasodium triphosphate, pentakalium triphosphate, polyphosphate; sodium polyphosphate, potassium polyphosphate, sodium calcium polyphosphate. calcium polyphosphate. tin-lf-chloride and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is selected from the group consisting of citric acid, formic acid; propanoic acid, lactic acid and sorbic acid, wherein the pet food composition does preferably not contain 1,2-dihydro-6-ethoxy-2,2,4-trimethylquinoline.
[93] The invention envisages a pet food composition comprising 0 at least one natural antioxidant, wherein the at least one natural antioxidant is selected from the 0 group of tocopherol and/or ascorbic acid and/or a plant extract, wherein the plant extract comprises oregano; salvia and/or rosemary extract and it) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is selected from the group consisting of bu thyI hydroxyani sot, hu thyihydroxy tofu ol propy 1 -3, 4. 5-trihydroxybenzoate, sulfur dioxide, sodium sulfite, sodium hydrogensulfite, sodium disulfite, potassium disulfite, calcium sulfite, calcium hydrogensulthe, potassium hydrogen sulfite, sodium 1..,-ascorhate, calcium Li-aseorbate, esters, octygallate, tertiary liiittylihydrocuinone (1713HQ), citric acid salts, sodium citrate, potassium citrate, calcium disodium EDTA, diphosphates, disodium diphosphate; trisodium diphosphate, tetrasodium diphosphate, IDiphosphate di ph osph ate, calcium diphosphate, triphosphates, pentasodium triphosphate; pentakalium triphosphate, polyphosphate. sodium polyphosphate, potassium polyphosphate, sodium calcium polyphosphate, calcium polyphosphate; tin-11-chloride, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is selected from the group consisting of citric acid, formic acid, propanoic acid, lactic acid and sorbic acid, wherein the pet food composition does preferably not contain 1,2-dilinicfro-6-ethoxy-2,2,4-ttim et ninoline.
[94] The invention envisages a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol, and ii) at least one synthetic antioxidant, Me at least one synthetic antioxidant is selected from the group consisting of buthylhydroxyanisol, buthylhydroxytoluol, propy1-3, 4, 5-aihydroxybenzoate, sulfur dioxide, sodium sulfite, sodium hydrogensulfite, sodium disunite, potassium disunite; calcium sulfite, calcium hydrogensuifite, potassium hydrogen sulfite, sodium L-ascorbate, calcium 1.,-ascorhate, octygallate, tertiary butyihydroquinone (IBH(2), citric acid salts, sodium citrate, potassium citrate, calcium disodium EDTA, diphosphates, disodium diphosphate, trisodium diphosphate, tetrasodium diphosphate, D phosphate diphosphate, calcium diphosphate, triphosphates, pentasodium tripbosphate, pentakalium triphosphate, polyphosphate, sodium polyphosphate, potassium polyphosphate, sodium calcium polyphosphate, calcium polyphosphate, tin-II-chloride, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is selected from the group consisting of citric acid, formic acid; 0 propanoic acid, lactic acid and sorbic acid, wherein the pet food composition does preferably not contain 1,2--di ethoxy-2,2,4--tri in ethyl qui n ol ne.
[096] The invention encompasses a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol in a ratio of 1:04. to 1:5, and ill at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is selected from the group consisting of buthylhydroxyarasol, buthythydroxytoluol" propy1-3, 4" 5-trihydroxybenzoate, sulfur dioxide, sodium sulfite, sodium hydrogensulfite" sodium di sul fite, potassium di su fi te, calcium sulfite, calcium hydrogen sulfi te, potassium hydrogen sulfite, sodium L-ascorbate, calcium Trascorhate, esters, octygallate, tertiary butylhydroquinone (TBHQ), citric acid salts, sodium citrate, potassium citrate, calcium disodium EDTA, diphosphates, disodium diphosphate, trisodium diphosphate, tetrasodium diphosphate, Diphosphate diphosphate, calcium diphosphate, triphosphates, pentasodium triphosphate, pontakalium triphosphate, polyphosphate, sodium polyphosphate, potassium polyphosphate, sodium calcium polyphosphate; calcium polyphosphate, tin-II-chloride, and iii) at least one acidity regulator component to regulate the pH, wherein the at least One Acidity regulator component is selected from the group consisting of citric acid, formic acid, propanoic acid, lactic acid and sorbic acid, wherein the pet food composition does preferably not contain1,2-dihydra-6-ethoxy-2,2,4-trimethylquinoline.
[096] The invention envisages a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is selected from the group of toecipherol and/or ascorbic acid and/or a plant extract, and ii) at least one synthetic antioxidant, wherein the at least one svnthetc antioxidant is selected from the group consisting of buthythydroxyanisol, huthythydroxytOluOl" propy1-3, 4, 5-trihydroxybenzoate, sulfur dioxide, sodium sulfite, sodium hydrogensulfite, sodium disulfite, potassium disunite, calcium sulfite, calcium hydrogensulfite, potassium hydrogen sulfite, sodium 1-ascorhate, calcium trascorbate, esters, octygallate, tertiary butylhydrocannone (IBHQ), citric acid salts:, sodium citrate, potassium citrate, calcium disodium EDTA, diphosphates, disodium diphosphate, trisodium diphosphate" tetrasodiffill diphosphate, Diphosphate diphosphate, calcium diphosphate, triphosphates, pentasodium triphosphate, pentakalium triphosphate, 0 polyphosphate, sodium polyphosphate, potassium polyphosphate, sodium calcium polyphosphate, calcium polyphosphate, tin-n-chloride, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one a.cidity regulator component is in the composition in an amount from 0.001% to 10% by weight of the composition, wherein the pet food composition does preferably not contain 1,2-dihydre-6-ethoxy-2,2,4-trimethylquinciline.
[097] The invention envisages a pet food composition, I) at least one natural antioxidant, wherein the at least one natural antioxidant selected from the group of;Decipher& and/or ascorbic acid and/or a plant extract,wherein the plant extract comprises oregano, :salvia and/or rosemary extract and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is selected from the group consisting of buthylhydroxyanisol, buthythydroxytoluoi, propy1-3, 4, 5-trihydrexybenzoate, sulfur dioxide, sodium sulfite, sodium hydrogensuifite, sodium disultite, potassium disunite, calcium sulfite, calcium hydrogensulfite, potassium hydrogen sulfite, sodium trascorhate, calcium L-a.scorbate, esters, octygallate, tertiary butylhydroquinone (TBHQ), citric acid salts, sodium citrate, potassium citrate, calcium di sodium EDTA, diphOsphates, di sodium di p hosp h ate, tri sodium diphosphate, tetrasodium diphosphate, Diphosphate diphosphate, calcium diphosphate, triphosphates" peniasodnim triphosphate, penta,kalium triphosphate, polyphosphate, sodium polyphosphate, potassium polyphosphate, sodium calcium polyphosphate, calcium polyphosphate, tin-11-chloride" and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is in the composition in an amount from 0.001% to 10% by weight of the composition, wherein the pet food composition does preferably not contain 1,2- tilt-1yd ro-6- [098] The invention envisages a pet food composition comprising i) at least one natural antioxidant. =rein the at least one natural antioxidant is a combination of rosemary extract and tocopherol, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is selected. from the group consisting of buthylhydroxyanisol, buthyl hydroxytoluol, propy1-3, 4, 5-trihydroxybenzoate" sulfur dioxide, sodium sulfite, sodium hydrogensulfite, sodium disulfite, potassium disulthe calcium sulfite, calcium hydrogensuifite, potassium hydrogen sulfite, sodium L-ascorbate, calcium L-ascothate, esters, octvgallate, butyihydroquinone (TF311Q1), citric acid salts, sodium citrate, potassium citrate, calcium disodium EDIA, dip,hosphates, disodium diphosphate, tri sodium diphosphate, tetrasodiurn diphosphate, Diphosphate diphosphate, calcium diphosphate" triphosphates, penta.sodium triphosphate, pentakaliont triphosphate, polyphosphate, sodium polyphosphate, potassium polyphosphate, sodium calcium polyphosphate, calcium polyphosphate, tin:II-chloride, and iii) at least one acidity regulator component to regulate the 1ilL wherein the at /east one acidity regulator component is in the composition in an amount from 0.001% to 10% by weight of the composition, wherein the pet food composition does preferably not contain 1,2-di hydro46-ethoxy-2,2, 4-tri m ethyl quinoli ne.
[099] The invention encompasses a pet food composition comprising 0 at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol in a ratio of 1:0.1 to 1:5, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is selected from the group consisting of buthyihydroxyanisol., buthylhydroxytoluol, propy1-3, 4, 5-trihydroxyhenzoate, sulfur dioxide, sodium sulfite, sodium hydrogensulfite, sodium di sul fi te, potassium di sill fi te, calcium sulfite, calcium hydrogensulfi te, potassium hydrogen sulfite, sodium L-ascorbate, calcium L-ascorbate, esters, octvgallate, tertiary butylhydroquinone (1U3t19), citric acid salts" sodium citrate, potassium citrate, calcium disodium EDT& diphosphates, disodium diphosphate, trisodium diphosphate, tetrasodium diphosphate, Diphosphate diphosphate, calcium diphosphate, triphosphates, pentasodium triphosphate, pentakalium triphosphate, polyphosphate, sodium polyphosphate, potassium polyphosphate, sodium calcium polyphosphate; calcium polyphosphate, tinll-chloride, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is in the composition in an amount from 0,001 to 10% by weight of the composition, wherein the pet food composition does preferably not contain 1,2-di hydro-6-ethoxy-2,2,4-tri methyl qui nal i ne.
[100] The invention envisages a pet food composition comp. 1) at least one natural antioxidant, wherein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a plant extract, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is Selected from 0 the group consisting of buthylhydroxyanisol, buthythydroNytoluol, propy1-3, 4, 5-trihydroxyhenzoate, sulfur dioxide, sodium sulfite, sodium hydrogensulfite, sodium di sulfite, potassium di sulfite, calcium sulfite, calcium hydrogensulfite, potassium hydrogen sulfite, sodium L-ascorhate, calcium L-ascorbate, esters, octygallate, tertiary butylhydroquinone (TBHQ), citric acid salts; sodium citrate, potassium citrate, calcium disodium EDTA, diphosphates, disodium diphosphate, trisodium &phosphate:, tetrasodium diphosphate, Diphosphate dipiTosphate, calcium diphosphate" triphosphates, pentasodium tripnosphate, pentakalium triphosphate, polyphosphate, sodium polyphosphate, potassium polyphosphate, sodium calcium polyphosphate, calcium polyphosphate, tinIl-chloride, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is citric acid, wherein the pet food composition does preferably not contain di hydro--6-ethoxy--2,2" --tri methyl qui n ine.
[101] The invention envisages a pet food composition comprising i) ai. least One natural antioxidant, wherein the at least one natural antioxidant is selected from the group of toeopherol and/or ascorbic acid and/or a plant extract; wherein the plant extract comprises Oregano, salvia and/or rosemary extract and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is selected from the group consisting of buthylhydroxyanisol, buthylbydroxytoluol, propy1-3,, 4, 5-trihydroxybenzoate, sulfur dioxide, sodium sulfite, sodium hydrogensuffite, sodium disulate, potassium disulthe, calcium sulfite, calcium hydrogensulfite, potassium hydrogen sulfite, sodium L-ascorbate, calcium L-ascorbate, esters, octygallate, tertiary butylhydroquinone (TBHQ), citric acid salts, sodium citrate, potassium citrate, calcium disodium EDT& diphosphates, disodium diphosphate, trisodium diphosphate, tetrasodium diphosphaie, Diphosphate diphosphate" calcium diphosphate, triphosphates, pentasodium triphosphate, pentakalium triphosphate, polyphosphate, sodium polyphosphate, potassium polyphosphate, sodium calcium polyphosphate, calcium polyphosphate; tin-11-chloride, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is citric acid, wherein the pet food composition does preferably not contain I,2-dihydro-6-ethoxy- -trimethylquinoline.
[102] The invention envisages a pet food composition COITI sing 0 at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of 0 rosemary extract and tocopherol, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is selected from the group consisting of buthylhydroxyanisol, buthylhydroxytoluol; propy1-3, 4, 54rihiydroxybenzoate, sulfur dioxide, sodium suifite. sodium hydrogensullite, sodium disullite, potassium disulfite, calcium sulfite, calcium hydrogensulfite, potassium hydrogen sulfite; sodium Irascorbate, calcium L-ascorhate, esters, octygallate, tertiary butythydroquinone (113HQ), citric acid salts, sodium citrate; potassium citrate, calcium disodium EDTA, diphosphates, di sodium diphosphate, trisodiurn diphosphate, tetrasodium diphosphate Diphosphate diphosphate, calcium diphosphate, triphosphates., pentasodium triphosphate, pentakalium triphosphate, polyphosphate; sodium polyphosphate; potassium polyphosphate, sodium calcium polyphosphate, calcium polyphosphate, tin-II-chloride, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is citric acid, wherein the pet food composition does preferably not contain 1,2-dihydro-6-ethoxy-2,2,4-trimethylquinoline.
The invention encompasses a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol in a ratio of 1:0.1 to 1:5, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is selected from the group consisting of buthylhydroxyanisol, buthylhydroxytolool, propy1-3, 4, 5-tiihydroxybenzoate, sulfur dioxide, sodium sulfite; sodium hydrog,ensulfite, sodium disulfite, potassium disultite, calcium Sulfite, calcium hydrogensulfite, potassium hydrogen sulfite, sodium L-ascorbate, calcium 1,-a.scorbate, esters, octygallate, tertiary butylhydroquinone (TBHQ); citric acid salts, sodium citrate, potassium citrate, calcium disodium A, diphosphates" disodium diphosphate, trsoduni diphosphate, tetrasodium diphosphate, Diphosphate diphosphate, calcium diphosphate, triphosphates, pentasodium triphosphate, pentakalium triphosphate, polyphosphate, sodium polyphosphate, potassium polyphosphate, sodium calcium polyphosphate, calcium polyphosphate, tin-it-chloride, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is citric acid, wherein the pet food composition does preferably not contain 122-dihydro-6-ethoxy-2,2,4-tritnethyiquinoline.
i.o [104] The invention envisages a pet food composition comprising at least one natural antioxidant, wherein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a plant extract, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is in the composition in an amount from 0.001% to 1,0% based on the crude fat content of the pet food composition and iii) at least one acidity regulator component to regulate the pH, wherein the at leak one acidity regulator component is selected from the group consisting of citric acid, fbrmic acid, propanoic acid, lactic t acid, wherein the pet food composi don does preferably not contain 1,2-dihydro-6-ethoxy-2,2,44rimethylquimatine.
[106] The invention envisages a pet food composition comprising i) at least one natural antioxidant, wherein the a.t least one natural antioxidant is selected from the group of tocopherbl and/or ascorbic acid and/or a plant extract, wherein the plant extract comprises oregano, salvia and/or rosemary extract and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is in the composition in an amount from 0.001% to 1.0% based on the crude fat content of the pet food composition, and Hi) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is selected from the group consisting of citric acid, formic acid, propanoic acid, lactic acid and sorbic acid, wherein the pet food composition does preferably not contain 1,2-di hydra-6-ethoxy-2,2,4-tri methyl qui n i n [106] The invention envisages a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxi ciant is in the compositlon in an amount from 0.00111-ti to 1.0% based on the crude fat content of the pet food composition and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is selected from the group consisting of citric acid, mic acid, propanoic acid., lactic acid and sorbic acid, wherein the pet food composition does preferably not contain 1,2-di Fly dro-6-ethoxy-2,2,4-trimethylquinoline.
[107] The invention encompasses a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of 0 rosemary extract and tocopherol in a ratio of 1:0:1 to 1:5, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is in the composition in an amount from 0.001% to 1.0% based on the crude fat content of the pet food composition. and in east one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is selected from. the group consisting of citric acid, formic acid, propanoic acid, lactic acid and sorbic acid, wherein the pet food composition does preferably not contain 1,2-dthydro-6-eth oxy-2,2,4-trimethyl qui nol Me.
[108] The invention envisages a pet food composition comt ng i) at least one natural antioxidant, wherein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a plant extract, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is in the composition in an. amount from 0 001% to 1.0% based on the crude fat content of the pet food composition and iii) at least one acidity-regulator component to regulate the pH, wherein the at least one acidity regulator component is in the composition in an amount from 0.001% to 10% by weight of the composition, wherein the pet food composition does preferably not contain 1.2hydr0-6-ethoxy-2,2,4-trimethyiquinoline [109] The invention envisages a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a plant extract, wherein the plant extract comprises oregano sa and/or rosemary ex-tract and m at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is in the composition in an amount from 0001% to 1.0% based on the crude fat content of the pet food composition, and id) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is in the composition in an amount from 0.001% to 10% by weight of the composition; wherein the pet food. composition does preferably not contain 1,2-dihydro-6-ettioxy2,2,44ri m ethyl quinoline.
[110] The invention envisages a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol, and ii) at least one synthetic antioxidant, wherein 0 the at least one synthetic antioxidant is in the composition in an amount from 0.001% to 1.0% based on the crude fat content of the pet food composition and iii) at least One acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is in the composition in an amount from 0.001% to 10% by weight of the composition; wherein the pet food composition does preferably not contain I,2-dihydro-6-et hoxy-2,2,z1-tri methy to ainol ne.
[111] The invention encompasses a pet food composition comprising at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol in a ratio cif 1:0.1 to 1:5, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is in the composition in an amount from 0.001% to 1.0% based on the crude fat content of the pet food composition, and iii) at least one acidity regulator component to regulate the pH, Wherein the at least one acidity regulator component is in the composition in an amount, from 0 001% to 10% by weight of the composition, wherein the pet food composition does preferably not contain 1"2-dihydro-6-ethoxy- 2 2,4-trimethylquinoline.
[112] The invention envisages a pet food composition comprising at least one natural antioxidant, wherein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a. plant extract, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is in the conmosition in an amount from 0.001% to 1.0% based on the crude fat content of the pet food composition and ast one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is citric acid, wherein the pet food composition does pt. not contain 1,2-di 6 etho.-trimethyiquinoline.
[113] The invention envisages a pet food composition comprising t at least one natural antioxidant, wherein the a.t least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a plant extract, wherein the plant extract comprises oregano, salvia and/or rosemary extract and at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is in the composition in an amount from 0.001% to 1.0% based on the crude fat content of the pet food composition, and iii) at least one acidity regulator component to 0 regulate the pH, wherein the at least one acidity regulator component is Citric and, wherein the pet food composition does preferably not contain 1,2-dihydro--6-ethoxy2,2,74-trimethylquinoline.
[114] The invention envisages a pet food composition comprising 1 at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is in the composition in an amount from 0.001% to 1.0% based on the crude fat content of the pet food composition and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is citric acid, wherein the per food composition does preferably not contain 1,2-dihydro-6-ethoxy-2,2,4-trimethylquino1ine.
[115] The invention encompasses a pet food composition comprising it at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol in a ratio of 1:0.1 to 1:5, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is in the composition in an amount from 0.001% to 1.0% based on the crude fat content of the pet food compoSition, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is citric acid, wherein the pet food composition does preferably not contain 1,:2-di.hydro-6-ettioxy-meth yl oinoline.
[116] The invention envisages a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a plant extract, and ii) at least one 30 synthetic antioxidant, wherejn the at least one synth+.tic antioxidant is buthylhydroxvtoluol and/or propy1-3; 42 54rihydroxybenzoate, at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is selected from the group consisting of citric acid, formic acid; propanoic acid, lactic acid and sorbic acid, wherein the pet food composition does preferably not contain 1,2-si ihydro-6-ethoxy-2,2,4-triniethy I qui nol in e [117] The invention envisages a pet food composition comprising i) at least one natural antioxidant, *herein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a plant extract, wherein the plant 0 extract comprises oregano, salvia and/or rosemary extract and hit at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is huthyihydroxytoluol and/or propyl; 4; 5 dri hydroxybenzoate, and at least one acidity regulator component to regulate the pEL, wherein the at least one acidity regulator component is selected from the group consisting of citric acid, formic acid; propanoic acid, lactic acid and sorbic acid, wherein the pet food composition does preferably not contain 1,2-dihydro-6-ethoxy-2,2,4-trimethylquinoline [118] The invention envisages a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is buthyllaydroxytoluol and/or propy1-3, 4; 5- trihydroxybenzoate, and iii) at least one acidity regulator component to regulate the pH, *herein the at least one acidity regulator component is selected from the group consisting of citric: acid, formic acid, propanoic acid,, lactic acid and sorbic acid, wherein the pet food composition does preferably not contain 4,2-dihydro-6-ethoxy- 2,2,44rimethylquinoline.
[119] The invention encompasses a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol in a ratio of 1:0 1 to 1:5, and if) at least one synthetic antioxidant; wherein the at least one synthetic antioxidant is buthylhydroxylolucil and/or propvl3, 4, 54rihydroxybenzoate, and east one acidity regulator component to regulate the pH, wherein the at least one aeidity regulator component is selected from the group consisting of citric acid, formic acid, propanoic acid, lactic acid and sorbic acid, wherein the pet food composition does preferably not containi."2-dihydro- 2"2,4 [120] The invention envisages a pet food composition comprising i) at least one natural antioxidant, e the a.t least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a plant extract., and ii) at least one synthetic antioxidant, wherein least one synthetic antioxidant is buthylhydroxyanisol andlor propy1-3, 4, 5-trihydroxybenzoate; and " least one acidity regulator component to regulate the wherein the at least one acidity regulator component is selected from the group consisting of citric acid, formic acid, 0 propanoic acid, lactic acid and sorbic acid, wherein the pet -food composition does preferably not contain 1,2-di In,7dro-6-ethoxy-2,2,4-tri in ethyl qui n ol i ne.
[121] 'The invention envisages a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a plant extract, wherein the plant extract comprises oregano, salvia and/or rosemary extract and it) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is buthylhydroxyanisol and/or propy1-3, 4, 54rihydroxybenzoate, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is selected from the group consisting of citric acid, formic, acid, propanoic acid, lactic acid and sorbic acid, wherein the pet food composition does preferably not contain 1,2-di hydro-6-ethoxy-2,2,44ri m ethylq uinoline.
[122] The invention envisages a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is buthylhydroxyanisol and/or propy1-1 trihydroxybenzoate, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is selected from the group consisting of citric acid, formic acid, propanoic acidlac-tic acid and sorbic acid, wherein the pet food composition does preferably not contain I,2--dihydro-6---ethoxy--m ethyl qui noi in e, (123] The invention encompasses a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol in a ratio of 1:01 to IS, and ii) at least one synthetic antioxidant, wherejn the at least one synthetic antiox.jdant is buthylhydroxyards61 and/or prop="4-3, 4, 54rihydroxyberizoate, and least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is selected from the group consisting of citric acid, formic acid, propanoic acid, lactic acid and sorbic acid, wherein the pet food composition does preferably not contain 1..7d -Ay -2,24-triniethylqui nol in e [124] The invention envisages a pet food composition COITI i) at least one natural antioxidant, wherein the at least one natural antioxidant selected from the 0 group of tocopherol and/or ascorbic acid and/or a plant extract, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is buthythydroxytoluol and/or propy4-3, 4 5-trihydroxybenzoate, and at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is in the composition in an amount from 0_001% to 10% by weight of the composition, wherein the pet food composition does preferably not contain 1"2-di hydro-6-ethoxy-2,2"4-1rimethylquinoline.
[125] The invention envisages a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a plant extract, wherein the plant extract comprises oregano, salvia and/or rosemary extract and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is buthylhydroxytolual and/or propyl--3, 4, 5-trihydroxybenzoate, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is in the composition in an amount from 0.001% to 10% by weight of the composition, wherein the pet food composition does preferably not contain 1,2-di hy dro-6-ethOxy-2,2,4-tri meth y;Jul [126] The invention envisages a pet food composition comprising at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is buthylhydroxytoluol and/or propylil trihydroxybenzOate, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is in the composition in an amount from 0.001% to 10% by weight of the composition, wherein the pet food position does prefera not contain I,2-di:o 6 -tho.-trimerhyiquinoline.
[127] The invention encompasses a pet food composition comprising id at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol in a ratio of 1:0.1 to 1:5, and Ii) at least one synthetic antioxidant, wherein least one synthetic antioxidant is buthylhydroxytoluol and/or propy 5-trihydroxybenzoate, and least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is in the composition in an amount from 0.001% to 10% by weight of the composition, wherein the pet food composition does preferably not contain 1,2-dihydro-6-ethoxy-2,2,4-trimethylquinoline [128] The inventio.n envisages a pet food composition comprising at least one natural antioxidant, wherein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a plant extract: and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is buthythydroxyanisol and/or propy1-3, 4, 54rihydroxybenzoate" and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is in the composition in an amount from 0.001% to 10% by weight of the composition, wherein the pet food composition does preferably not contain 1,2-dihydro-6-ethoxy-2,2,4-trimethylquinoline.
[129] The invention envisages a pet food composition comprising ij at least one natural antioxidant, wherein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a plant extract, wherein the plant extract comprises oregano, salvia and/or rosemary extract and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is buthythydroxyanisol and/or propy1-3, 4, 5-trihydroxybenzoate, and iii) at least one acidity regulator component to regulate the p.H, wherein the at least one acidity regulator component is in the composition in an amount front 0.001% to 10% by weight of the composition, wherein the pet food composition does preferably not contain I,2-di hydro-6-ethOxy-2,2,4-tri meth yi tit i [130] The invention envisages a pet food composition comprising at least one natural antioxidant wherein the at least one natural antioxidant is a combination of rosemary extract and tocooheml, and ii at least one synthetic ant oxidant" wherein the at least one synthetic antioxidant is buthylhydroxyanisdl and/or propyi-3, 4, 5-trihydroxybenzoate, and Ili) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is in the composition in an amount from 0.001% to 10% by weight of the composition, wherein the pet food composition does preferably not contain 1,2-di hydro-6-ethoxy-2,2,4-trimethylquinoline.
[131] The invention encompasses a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of 0 rosemary extract and tocopherol in a ratio of 1:0:1 to 1:5, and) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is buthythydroxyanisol and/or propy1-3, 4, 5-trihydroxyberizoate, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is in the composition in an amount from 0_001% to 10% by weight of the composition, wherein the pet food composition does preferably not contain 1"2-di hydro-6-ethoxy-2,2"4-trimethy.1quinoline.
[132] The invention envisages a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a plant extract, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is buthylhydroxytalual and/or propy1-3, 4, 5-trihydroxyhenzoate, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is citric acid, wherein the pet food composition does preferably not contain 1,2-dihydr0-6-ethoxy-2,2,4-trimethylquinoline.
[133] The invention envisages a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant ected from the of tocopherol and/or ascorbic acid and/or a plant extract, wherein the plant extract comprises oregano, and/or rosemary extract and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is buthylhydroxytoluol and/or prom. 4 5-trihydroxybenzoate, and at least one acidity regulator component to regulate the wherein the a.t least one acidity regulator component is citric acid, wherein the pet food composition does preferably not contain 1,2-dihydro-6-ethoxy-2,4-trime lquinoline.
[134] The invention envisages a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is buthylhydroxytolnol and/or propy1-3, 4, 5-trihydroxybenzome, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is citric acid, wherein the pet food composition does preferably not contain 1,2-dihydro-6-etboxy-2,2,4-mthylquinoline.
[135] The invention encompasses a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol in a ratio of 1:0.1 to 1:5, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is buthylhydroxytoluol and/or propy1-3, 4, 5-trilaydroxybenzoate, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is citric acid, wherein the pet food composition does preferably not contain 1,2-dihydro-6-ethoxy-2"2,4-trimethylquinoline.
[136] The invention envisages a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a plant extract, and ii) at least one synthetic antioxidant, Wherein the at least one synthetic antioxidant is butbylbydroxyanisol and/or propy1-3, 4, 54rihydroxybenzoate, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is citric acid, wherein the pet food composition does preferably not contain 1,2-dihydro-6-ethoxy-2,2,4-trimethylquinoline.
[137] The invention envisages a pet food composition comprising at least one natural antioxidant, wherein the at least one natural antioxidant is selected from the group of tocopherol and/or ascorbic acid and/or a. plant extract, wherein the plant extract comprises oregano, saivia andlor rosemary extract and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is buttrelhydroxyanisol and/or propyl-.3, 4, 54rihydroxybenzoate, and iii) m least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is -laic acid, where.in he pet food composition does preferably not contain 1,2-dihydro-6-ethoxy-2,2,4strimethylquinoline.
[138] The invention envisages a pet food composition comprising i) at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol, and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is buthyl hydroxyanisoi and/&r propy1-3, 4, .5-trihydroxybenzoate, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is citric acid, wherein the pet food composition does preferably not contain 1,2-dihydro-osethoxy-2,2,4- 0 trimethylquirioline: [139] The invention encompasses a pet food composition comprising l) at least one natural antioxidant, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol in a ratio of 10.1 to 1:5.. and ii) at least one synthetic antioxidant, wherein the at least one synthetic antioxidant is buthylhydroxyanisial andlor propy1-3, 4, 5-trihydrox.ybenwate, and iii) at least one acidity regulator component to regulate the pH, wherein the at least one acidity regulator component is citric acid,, wherein the pet food composition does preferably not contain 1,2-di hy dro-6-ethoxy-2,2,4-tri trt ethyl qui noline.
[140] Preferably, the invention comprises the pet food composition, wherein i) the at least one natural antioxidant is a combination of rosemary extract and tocopherol in a ratio of 1:0,1 to 1:5, ii) the at least one synthetic antioxidant is huthylhydroxyiblual and/or propy1-34,5strihydroxybenzoate, and ill') the at least one acidity regulator component is citric acid, wherein the pet food composition does preferably not contain,2-dihydro-ô-ethoxy-2,2,4trimethylquinoline [141] The term "1,2-dihydro-6-ethoxy-2,244rimethylquinolin refers to ethoxyquin EQ), which is a quinoline-ba.sed synthetic antioxidant used as a food preservative in certain countries. tt is used as a preservative in some pet foods to prevent the rancidification of fats. Preferably: fish meal is protected with the synthetic antioxidant. It has been speculated that ethoxyqum in pet foods such as in fish meal might be responsible for multiple health problems, for the animal itself and for the consumer eating the animal (such as fish). Thus, food safety concerns have been raised to reduce maximal levels of synthetic antioxidants such as etbox:yquin allowed in food for human consumption (Bohne et al. 2008, Foca and Che.micat Thx/coiogy, 46, 43 [142] The pet food composition may further comprise a dietary component selected from the group consisting of a fat, an oil, a protein, a vitamin, a starch, a flavoring agent and a coloring agent. The pet food composition may further comprise a dietary component, which comprises (a) fat(s) and (an) oil(s) and (a) protein(s) and (a) vitamin(s) and (a) starch(es) and (a) flavoring agent(s) and (a) coloring agent(s). The pet food composition may further comprise a dietary component, which comprises (M fat(s) or (an) oil(s) or (a) protein(s) or (a) 0 vitamin(s) or (a) starch(es) or (a) flavoring agent(s) or (a) coloring agent(s4 [143] The pet food may comprise fat(s) and oil(s). Fat include animal fats such as tallow, or vegetable fats such as hydrogenated soy fat. Any suitable oil may be used for example vegetable oils such as sunflower oil, safflower oil, corn oil, and the like. Oils which are high in mono-d fats are particularly preferred. Hydrogenated oils or fats are also preferred. Liquid fats and oils form the lipophilic matrix of the pet food coin posi Lion.
[144] Further, the pet food comprises protein(s). Suitable protein sources may he selected from any suitable animal or vegetable protein source; for example meat meal, bone meal, fish meal, soy protein concentrates, milk proteins, gluten, and the like. The choice of the protein source will be largely determined by the nutritional needs, palatability considerations, and the type of cereal product produced. Of course, the starch source may also be a source of protein.
[145] Vitamin(s), generally useall as food additives, is/are also comprised in the pet food composition, including vitamin A, vitamin [31" vitamin B2, vitamin 136, vitamin 1312, vitamin D. biotin, vitamin K, folic acid, inositol, niacin, and pantathenic acid. Minerals and trace elements also useful as food a.dditives include calcium, phosphorus, sodium, potassium, magnesium, copper, zinc, chloride, iron, iodine, and iron and may also be added to the pet food composition.
[146] Suitable starch sources are, for example, grain flours such as corn, rice, wheat, beets; barley, soy and oats. Also mixtures of these flours may be used. The flouts may be whole flouts or may be flouts which have had fractions removed; felexample the get-n:i fraction or husk fraction may be removed. Rice flour, corn flour and wheat flour are particularly suitable; either alone or in combination. The starch source will be chosen largey on the basis of the n ict e. path considerations, and the type of pet food desired.
[147] Suitable particulate flavouring agents include shrimp and hill meal,sugars, chocolate powders, milk powders, malted powders, flavored beverage powders, and the like. Coloring agents include for example carotinoids and algae. Proteins; vitamins, minerals, starch, flavouring, coloring agents and the like form the solid matrix of the pet food composition.
[148] The pet food composition is primarily made up of the starch source and other ingredients such as sugar, salt, spices, seasonings; vitamins, minerals, fillers, binding 0 agents, flavoring agents, coloring agents, emulsifiers, sweeteners, buffers, antioxidants, protein sources, fats, oils and the like known to skilled artisans. Specific amounts for each composition component, food ingredient, and other ingredients may depend on a variety of factors such as the particular components and ingredients included in the composition; the species of animal; the animal's age; body weight, general health, sex, and diet: the animal's consumption rate: and the like.
[149] Additionally, the pet food composition y comprise a wet or dry food composition, which may be in the form of a moist food, semi-moist food, dry food; supplement or treat. The pet food composition may be in kibble form, flakes, pellets, sticks, granules, gelthod or tablets and the like. Preferably, the pet food composition is dry food.
[150] In a preferred pet food composition the at least one natural antioxidant and the at least one synthetic antioxidant are integrated homogeneously into a lipophilic matrix, before obtaining the pet food composition.
[151] Preferably, the additives (natural and synthetic antioxidants) are mixed into the lipophIlic matrix thereby obtaining an enriched lipophilic matrix. The acidity regulator component is mixed into a premix thereby obtaining an enriched premix. Said enriched lipophilic matrix and said enriched premix are then homogeneously dispersed into a solid matrix. Subsequently, processing into the pet food composition occurs.
[152] In this context, the term "premix' refers to a mixture of solids comprising a carrier being a substance that is already included in the main mix (the pet food composition) and comprising microcomponents. Microcomponents are substances whose amount in the formulation is less than 0.5% Microcomponents are usually ins,trace elements, minerals and additives, preferably the at least one acidity regulator component to regulate the pH, more preferably citric acid. Thus, the premix may comprise a carrier and citric acid. The microcomponent such as citric acid may homogeneously be mixed into the carrier, resulting in the premix. Here, the carrier used in the premix is not the same carrier as described in [39].
[153] The term "integrated" means dispersed, distributed or mixed. In this context, the term "homogenouslv" means that the at least one natural antioxidant and the at least one synthetic antioxidant and the at least one acidity regulator component that 0 is (dis)solved and/or dispersed in the lipophilic matrix is nearly evenly, preferably evenly, distributed in the lipophilic matrix so that the concentration ("c") of the at least one natural antioxidant and the at least one synthetic antioxidant and the at least one acidity regulator component ("n" in case of molar mass or "in" in case of mass) is nearly identical, preferably identical in (or throughout) the volume ("y") of the lipophi lie matrix, i.e., c=niliv or C=111/V, respectively, is nearly constant, preferably constant. Preferably there is no concentration gradient within the pet food positi [154] The invention also comprises the integration of the enr died lipophilic matrix (enriched through. the additives such as natural and synthetic antioxidants being mixed into the lipophilic matrix) into the pet food composition after processing the solid matrix into the pet food composition. This may occur through coating, spraying and/or vacuuming.
[155] The pet may be a vertebrate and/or invertebrate. Vertebrates consist of "lower vertebrates" such as fishes and amphibians and "higher vertebrates" such as reptiles, birds and mammals. The vertebrates of the invention include higher vertebrates, which dude, but are not limited to all kinds of mammals such as a cat, a kitten, a dog, a puppy, a horse, a mouse, a hamster, cattle, a pig, a sheep and the hike. The vertebrates of the invention include lower vertebrates as well, which include, but are not limited to all kinds of fish.
[156] Preferably, the vertebrate is a lower vertebrate, preferably a fish, more preferably a bony fish (Osteichthyes), most preferably an ornamental fish belonging e.g. to the family of chara.cins, barbs, cichlids, live bearers, cyprinids. In a preferred embodiment the pet is a red phantom tetra and/or a yellow cichlid (tabidochrom [157] Additionallj,/ the pet food composition of the invention may be palatable for fish. Thus, the invention comprises a pet food composition, wherein the pet food composition may be palatable for fish. Palatablility of the pet food composition may be achieved through mixing the additives (the at least one natural antioxidant and the at least one synthetic antioxidant oldie invention) into a lipophilic matrix before obtaining the pet food composition. Additionally, palatability of the pet food composition is preferably be achieved by mixing the at least one acidity regulator component used for the acceptance and attractiveness enhancement, weight enhancement and optimized feed utilization into a premix, before the enriched lipophilic matrix and the emiched premix may homogeneously be dispersed into a solid matrix and then processed into the pet food composition of the invention.
[158] Further, the pet food composition enhances the weight in a fish. Thus, the invention comprises a pet food composition, wherein the pet food composition may enhance the weight in a fish. The weight increase of a fish pisces) due to more food intake of said aquatic subject, which finds the pet food very palatable by reason of its specifically composition according to the invention, is measured with a special digital scale with mode for moving animals, preferably suitable for fish.
[169] The tom "enhance/increase the weight", or "weight increase' refers to the increase/enhancement of the body weight of the pet, preferably of the fish. The increase of the body weight does not only refer to the increase of body fat. The pet's body (in particular the fish's body) becomes proportionally larger/bigger, meaning that there is an increase in muscle mass, organ growth and bone growth. The increased growth of the pet is defined by the weight increase. Thus, the term "growth" may be used interchangeably with the term "weight".
[160] Further, the pet of the invention may be an invertebrate. The invertebrate may be a deca.pod. The decapods are an order of crustaceans within the class Malacostraea. Decapods include many familiar groups, such as crayfish, crabs, Lobsters, prawns, and shrimp Method of producing a pet food composition [161] The invention also envisage a method of producing a pet food composition, the method comprising a) mixing the additives of a pet food composition comprising at least one natural antioxidant, and at least the antioxidant into a lipophilic matrix, b.) mixing at least one acidity regulator component to regulate the p1-i into a premix c) applying the enriched lipophilic matrix of step a) and the enriched premix of step b) to a solid matrix before Obtaining: the pet food composition, d) obtaining the pet food composition.
[162] The stabilization of the diet and of the lipophilic components can be carried out in the dietetic solid matrix prior to processing. In this case, the addition of the stabilizing components may be carried out via the lipophilic components. For this purpose, these stabilizing components (natural and synthetic antioxidants) may be 0 mixed into a lipophilic substance matrix.
Step a) refers to the homogeneously integration of the additives (natural and synthetic antioxidants) into a lipophilic matrix; thereby obtaining an enriched lipophilic matrix. 'Me additives used for the acceptance and attractiveness enhancement; weight enhancement and optimized feed utilization (such as the at least one acidity regulator component) may be mixed in step b) into a premix thereby obtaining, an enriched premix. Preferably, mixing of the at lea-st one acidity regulator component into the premix is achieved by homogenization in a normal mixer known to a person skilled in the art. After that; the enriched lipophilic matrix and the enriched premix may then be homogeneously dispersed into a solid matrix matrix). Homogeneously dispersing the enriched lipophilic matrix and the premix into the solid matrix may be achieved in a normal mixer again. Different mixers may be used depending whether a solid mixture for extrudates such as pellets or a. mash for the flake production may be produced. Subsequently, the pet food composition may be processed. Processing may take place in a flake drum (110- 160°C), in an extruder (80-180'C), in a tablet machine (18-40"C), in a pelletizer (20- 12("C). If moisture food and/or soft food may be processed, a temperature of I 8-180"C may be used. Temperatures may be dependent on the pet -food composition, which is obtained, whether dry pet food such as pellets and/or flakes may be produced or wet pet food.
Non-medical uses [163] The invention further encompasses the use of a pet food composition for improving the palatabiijtv of the pet food composition and/or for enhancing the weight in a healthy pet The invention further comprises the use of a pet food composition for improving the palatability of the pet food composition. The invention further encompasses the use of a pet food composition for enhancing the weight in a healthy pet. The invention further encompasses the use of a pet food composition for improving the palatability of the pet food composition and for enhancing the weight in a healthy pet. However, the same use of the pet food composition may also apply to a non-healthy pet.
[164] The term -enhancing" may be used interchangeably with the terin 0 or increasing.
[165] The term "a. healthy pet not suffering, from am disorders/diseases. Thus, the pet is hein [166] The synergistic effect of a combination of synthetic and natural antioxidants in a specific ratio, with the effect of a p1-Powering and thus acceptability-improving component (see diet SNC in Fig. 1) may increase the weight of aquatic subjects by up to 2.5%, or by pp to 3%'5% 4%, 43% 5.5%, 6%, 6.5%, 7%, "; 8%, 8.5%, 9%, 9.5%, 10%, 10.5%, 11%, 11.5%, 12. 12.5%, 13%, 13.5%, 14%, 145%, 15%, I 5"5%, 16%, 16.5%, 17%, 17.5%, 18%, 18.5%, 19%, 19.5%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or even by up to 100%. The term "by up to uiti" means an increase/decrease of the number indicated, not including 0% increase/decrease.
[167] Preferably, it increases the weight of lane subjects by up to 14% in comparison to the control diet without supplementation. Said combination may increase the weight of aquatic subjects by about 14% in comparison to the control diet without supplementation if the pet is a red phantom tetra (see diet SNC for the red phantom tetra. in Fig. 1). The addition of natural antioxidants (see diet NC for both targets in Fig.. 1) may adversely affect the weight of the individual species by up to 6% compared to the control diet, showing that only the addition of the natural antioxidants such as toeopherol and rosemary extract with the effect of the acidity regulator component citric acid may not be sufficient to make the pet food coniposidon more palatable and thereby increasing the weight Of the aquatic subjects. The addition of the synthetic antioxidants (see diet SC for both targets in 111g. 1) may not affect the weight of the individual species compared to the control diet, demons ruing that only the addition of the synthetic anuoxidanis such as buthylhydroxytoluol (or buthythydroxyanisol) and propy1-3, 4, 5-trihydroxybenzoate with the effect of the acidity regulator component citric acid may not be sufficient to make the pet food composition more palatable and thereby increasing the weight of the aquatic subjects.
[168] Additionally, the invention comprises the LISe of a pet food composition for protecting the pet food composition against oxidation. The additives of the invention may act as electron donors, which may prevent the release of elections of the dieiary components, which are being protected. Lipid degradation can take place chemical-by absorbing odors, biochemically by the activity of tissue enzymes or microorganisms, or by delivery of electrons to the oxygen atom. In detail, the release of electrons occurs to the oxygen atom, which has only a single unpaired valence electron, thus being very aggressive. "ate strong pressure of filling the outer shell with another electron to be captured makes these substances so aggressive_ Those aggressive oxygen atoms may react with e most diverse compounds and may destroy them by the reaction taking place [169] The unsaturated fatty acids of the diet n,t spontaneously react with atmospheric oxygen in a diet. As the number of double bonds increases, the tendency to release mmxc electrons also increases. In this process, peroxides and fr,tdroperoxides are first formed, and aldehydes, acids, and polymerization products, which characterize the degraded lipid in a sensory manner, are formed in secondary reactions. They are responsible for the typical, had taste of spoiled (degraded) lipids. The peroxides also inactivate certain nutrients, e.g. specific vitamins. As a result, the utilization of the diet deteriorates (the diet gets worse), the weight of the target species (e.., lower vertebrates) stagnates and further physiological damages to the target species occur. For this reason, electron donators are often added which intercept the primarily developing atoms or molecules with at least one unpaired valence electron, thereby interrupting the continuous emission of electrons from the unsaturated fatty acids. Thus, the spoilage of the dietary component may be prevented.
[170] Further, the invention envisages the use of a pet food composition for improving the water quality and reducing the growth of algae in the (artificial) habitat of a pet, preferably if the pet is a fish, more preferably if the pet is a bony fish, most preferably if the pet is an ornamental fish.
[171] The invention al SO comprises a method of improving the palatabiliw of a pet food composition, the method comprising, a) mixing the additives of a pet food composition comprising i) at least one natural antioxidant, and ii) at least one synthetic antioxidant into a lipophilic matrix, h) mixing the at least one acidity regulator component to regulate the pH into a premix thereby improving the palatability of a pet food composition in comparison to a premix not having applied the at least one acidity regulator component to, c) applying the enriched lipophilic matrix and the enriched premix to a solid matrix, optionally d) obtaining the pet 0 food composition.
[172] further, a method of protecting a pet food composition against oxidation is also contemplated by the invention, the method comprising, a) mixing the additives of a pet food composition comprising 1) at least: one natural antioxidant, and ii) at least one synthetic antioxidant into a lipophilic matrix, thereby protecting the oxidation of the lipophilic matrix in comparison to a liipophilic matrix not having applied the mixed additives to b) mixing the at least one acidity regulator component to regulate the pH into a premix, c) applying the enriched lipophilic matrix and the enriched premix to a solid matrix, optionally d) obtaining the pet food composition.
[173] A method of enhancing the weight in a pet comprising administering a pet food composition to the pet is also comprised by the invention. Also a method of enhancing the weight in a healthy pet comprising administering a. pet food composition to the healthy pet is comprised by the invention. However, the same method of enhancing the weight may also apply to a non-healthy pet.
Medical use [174] The invention also encompasses a pet food composition for se in a method of treating underweight in a pet. The term "weight deficiency' "short. weight" may be used interchangeably with the term "underweight". A pet suffering from underweight weighs less than is normal, healthy or required for said pet. Feeding the pet food composition of the invention to the pet suffering from underweight may be able to treat the limitation of said pet.
[175] is noted that as used herein, the singular forms 'a", and -the", include plural references -unless the context clearly indicates otherwise. Thus, for example, reference to "-a reagent' includes one or more of such different reagents and reference to "the method" includes reference to equivalent steps and methods known to those of ordinary skill in the art that could be modified or substituted for the methods described herein.
[176] Unless otherwise indicated, the term "at least" preceding a senes ot eiements is to be understood to refer to every element in the series. The term east one" 0 refers to one or more such as two, three, four, five, six, seven, eight, nine, or ten.
Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein Such equivalents are intended to be encompassed invention [177] Inc term "and/or" wherever used herein includes the meaning of ' and". cf and 'all or any other combination of the elements connected by said term".
[178] The term "less than" or in turn "more than" does not include the concrete number.
[179] For example, less than 20 means less than the number indicated. Similarly, 20 more than or greater than means more than or greater than the indicated number.
[180] Throughout this specification and the claims which follow, unless the context requires otherwise, the word "comprise", and variations such as "comprises" and "comprising", will be understood to imply the inclusion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or group of integer or step. When used herein the term "comprising" can be substituted with the term "containing" or "including' or sometimes when used herein, with the term "having". When used herein "consisting of excludes any element, step or ingredient not specified.
[181] The term ' tiding'' means udine but not limited to" "Maudlin_ "including hut not limited to" are used interchangeably.
[182] The term "about" means plus or minus 10%, preferably plus or minus 5%, more preferably plus or minus 2%, most preferably plus or minus [183] Throughout the description and claims of this specification, the singular 46 encompasses the plural unless the context otherse requirus. In particular, wheru the indefinite article is used, the specification is to be understood as contemplating plurality as well as singularity, unless the context requires otherwise.
[184] It should be understood that this invention is not limited to the particular methodology, protocols, material, reagents, and substances, etc., described heroin and as such can vary. The terminology used herein is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the invention, which is defined solely by the claims.
[185] All publications cited throughout the text of this specification (including all 0 patents, patent application, scientific publications, instructions, etc.), whether supra or infra, are hereby incorporated by reference in their entirety. Nothing herein is to be construed as an admission that the invention is not entitled to antedate such disclosure by virtue of nnor invention. To the extent the material incorporated by reference contradicts or is inconsistent with this specification, the specification will supersede any such material.
[186] 'The content of all documents and patent documents cited herein is. incorporated by reference in their entirety.
[187] A better understanding of the invention and of its advantages will be gained from the following examples, offered for illustrative purposes only. The examples are not intended to limit the scope of the invention in any way.
EXAMPLES OF THE INVENTION
[188] Earnpie I: Pet food composition as a Wet for lower vertebrates.
[189] The combination of the stabilizing agents (at least one synthetic antioddant and at least one natural antioxidant and at least one acidity regulator component; diet SNC in Fig. 1) of the invention in diets for lower vertebrates was tested for ornamental Osteichthyes (bony fishes such as the Red Phantom Tetra and the yellow Cichlid called Labidochromis* enehtletts), being characteristic for the aquatic habitat, in cold and warm water, in comparison to a control diet without any stabilizing agents (control diet) and to different test diets with synthetic antioxidants with a supplementation of a pH-lowering component (diet SC in Fig. 1) or with natural anti th a supplementation werin, component)t NC in Fig s each over a period of 12. weeks.
[190] As internal results of feeding test demonstrate, the synergistic effect of a combination of synthetic and natural antioxidants in a. specific ratio, with the effect of a pFfilowering and thus acceptability-improving component (diet SNC), increases the weight of the aquatic subjects (such as red phantom tetra arid/or yellow cichlid) by up to 14% in comparison to the control diet without supplementation. As the results also show, the addition of natural antioxidants (diet NC) adversely affects the weight of the individual species by up to 6% compared to the control (Fig. I).
The invention is also described by reference to the following clauses: A pet food composition comprising i) at least one natural antioxidant, and ii) at least one synthetic antioxidant; and iii) at least one acidity regulator component to regulate the pH, wherein the pet food composition does not contain 1,2-dilwdro6-ethoxy-2,2,4-trimethylgitinoline, 2. The pet food composition of clause 1, wherein the at least one natural antioxidant is selected from the group consisting of tocopherol, ascorbic acid, a plant extract and combinations thereof 3. The pet food composition of clause 2, wherein the plant extract comprises oregano, salvia, and/or rosemary extract.
4 The pet food composition of any one of clauses 1 to 3, wherein a.t least one natural antioxidant is a combination of rosemary extract and tocopherol 5. The pet food compositi iaise 4 iherein he ratio of rosen extract to toeopheroi is 1:0.1 to 1:5.
6. The pet food composition of any one of clauses 1 to 5, wherein he least one synthetic antioxidant is selected from the group consisting of buthylhydroxyanisol, butylhydroxytoluol, propy1-3,4,54rihydroxybenzoate, sulfur dioxide, sodium sulfite, sodium.hydrouensulfite, sodium disulfite, potassium disulfite, calcium sulfite, calcium hydrogensulfite, potassium hydrogen sulfite, sodium L-ascorbate, calcium 1-ascorbate, esters, octygallate, tertiary butyihydroquinone (TBI-1Q), citric acid salts, sodium citrate, potassium citrate, calcium disodium EDTA, diphosphates, disodium diphosphate, trisodium diphosphate, tetrasodium diphosphate. Diphosphate diphosphate, calcium diphosphate, triphosphates, pentasodiuni tri p h osph ate, pentakalium tri ph osph ate, polyphosphate; sodium polyphosphate, potassium polyphosphate, sodium calcium polyphosphate, calcium polyphosphate, ti n -II-chl on de and combinations thereof..
7 The pet food composition of any one of clauses 1 to 6, wherein the at least one synthetic antioxidant is in the composition in an amount from 0 001% to 1.0% based on the crude fat content of the pet food composition.
8. The pet food composition of any one of clauses 1 to 7, wherein the at least one acidity regulator component is selected from the group consisting of citric acid, formic acid, propanoic acid, lactic acid, sorbic acid and combinations thereof 9. The pet food composition of any one of clauses 1 to 8, wherein the at least one acidity regulator component is in the composition in an amount from 0.001% to 10% by weight of the composition.
the pet food composition of any one of clauses I to 9, wherein the pH ow 6.7.
11 The pet food composition of any_ one of clauses 1 to 10, wherein 20) the at east one natural antioxidant is a combination of rosemary extra- d tocopherol in a ratio of 1:0.1 to 1:5 ii) the at least one synthetic antioxidant is a combination of butylhydroxytoluol and/or propy1-3,4,5-trihydroxybenzoate, and iii) the at least one acidity regulator component is citric acid.
12. The pet food composition of any one of clauses 1 to 11, further comprising a dietary component selected from the group consisting of a fat, an oil, a protein, a vitamin, a starch, a flavoring agent, a coloring agent and combinations thereof 13. The pet food composition of any one of clauses 1 to 12, wherein the at least one natural antioxidant and the at least one synthetic antioxidant are integrated homogeneously into a lipophilic matrix.
I. A method of producing a pet food composition of any one of -1 to 13; the method comprising ait mixing the additives of a pet food composition comprising 0 at least one natural antioxidant, and ii) at least one synthetic antioxidant into a liponhilic matrix, b) mixing. the at least one acidity regulator component to regulate the pfi into a premix, c) applying the enriched lipop Hie matrix of step a) and the enriched premix of step b) to a so.id matrix before obtaining the pet food composition, d) obtaining the pet food composition.
5. Use of a pet food composition of any one of clauses 1 to 13 for improving the palatability of the pet food composition and/or for enhancing the weight in a healthy pet.

Claims (1)

  1. CLAIMSA pet food composition comprising S i) at least one natural antioxidant, and at least one synthetic antioxidant; and at least one acidity regulator component to regulate the Pft wherein the pet food composition does not contain 1,2-dihydro- 6-orhoxy-2 ri methy I qui noline.
    2, The pet food composition of claim I, wherein the at least one natural antioxidant is selected from the group consisting of tocopherol, ascotbie acid a plant extract and combinations thereof 3. The pet food composition of claim 2, wherein the plant extract col vises oregano, salvia, and/or rosemary extract.4. The pet food composition of any one of claims I to 3, wherein the at least one natural antioxidant is a combination of rosemary extract and tocopherol.The pet food composition of clam wherein the ratio of rosemary extract to tocopherol is 1:0T to 1:5, 6. The pet food composition of any one of claims I to 5, wherein the at least one synthetic antioxidant is selected from the group consisting of buthy by droxyani sol, butylhydroxytol uol, propy I -3,4,5-tri hydroxyl) zoate, sulfur dioxide, sodium sulfite, sodium hydrogensulfite, sodium disuifite, potassium disulfite, calcium sulfite, calcium hydrogensulfite, potassium hydrogen sulfite, sodium L-ascorbate, calcium L-ascorbate, esters; octygallate, tertiary butylhydroquinone (TB11()), citric acid salts, sodium citrate, potassium citrate, calcium disodium EDTA, diphosphates, disodium &phosphate, trisodium diphosphate, tetrasodium diphosphate, Diphosphate diphosphate, calcium diphosphate, triphosphates, pentasodium triphosphate, pentakalium triphosphate, polyphosphate, sodium polyphosphate" potassium polyphosphate, sodium calcium polyphosphate, calcium polyphosphate, tin-II-chloride and combinations of.7. The pet food composition of any one of claims 1 to 6, wherein the at least one synthetic antioxidant is in the composition in an. amount from 0.001% to 1.0% based on the crude fat content of the pet food composition.8. The pet food composition of any one of claims I to 7, wherein the at least one cidity regulator component is selected from the group consisting of citric acid, formic acid, propanoie acid, lactic acid, sorbic acid and combinations thereof 9. The pet foot composition of any one of claims 1 to 8, wherein the at least one acidity regulator component is in the composition in an amount from 0.001% to 100/0 by weight of the composition.10. The p food coinposi Lion oneof claims 1 to 9, vherein the pH below 6.7.The composition of any one of claims I to 10, wherein i) the at least one natural antioxidant is a combination of rosemary extract and copherol in a ratio of 1:0.1 to 1:5, iit the at least one synthetic antioxidant is a combination butylhydroxytoluol andlor propy1-3,4,5-trihydroxybenzoate, and iii) the at least one acidity regulator component is citric acid.food composition of any one of Ciaitl'IS I to 11, further comprising a dietary component selected from the group consisting of a fat, an Oil, a protein, a vitamin, a starch, a flavoring agent a coloring agent and combinations thereof, 13. The pet food composition of any one of claims 1 to 12, wherein the at least one natural antioxidant and the at least one synthetic antioxidant are integrated homogeneously into a lipophilic matrix.14. A method of producing a pet food composition of any one of claims 1 to 13, the method comprising mixing the additives of a pet food composition comprising lea.st one natural antioxidant, and at least one synthetic anti into a lipophilie matrix, mixing the at least one acidity reguator component to regulate the p11 into a premix, c) applying the enriched lipophilic matrix of step a enriched premix of step b) to a solid matrix before obtaining the pet food composition, :1.0 d) obtaining the pet food composition.15. Use of a pet food composition of any one of claims 1 to 13 for improving the palatability of the pet food composition and/or for enhancing the ''eight in a healthy pet.
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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011127163A1 (en) * 2010-04-09 2011-10-13 Martek Biosciences Corporation Thermally stable oil-in-water emulsions containing an oil that contains polyunsaturated fatty acids
US20130059057A1 (en) * 2010-05-25 2013-03-07 Kao Corporation Pet food
US20130251865A1 (en) * 2011-09-09 2013-09-26 Kemin Industries, Inc. Antioxidant Formulations

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011127163A1 (en) * 2010-04-09 2011-10-13 Martek Biosciences Corporation Thermally stable oil-in-water emulsions containing an oil that contains polyunsaturated fatty acids
US20130059057A1 (en) * 2010-05-25 2013-03-07 Kao Corporation Pet food
US20130251865A1 (en) * 2011-09-09 2013-09-26 Kemin Industries, Inc. Antioxidant Formulations

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