GB2612747A - Characteristic peptide segment capable of distinguishing deer horn gelatin from deer skin gelatin, and test method therefor - Google Patents
Characteristic peptide segment capable of distinguishing deer horn gelatin from deer skin gelatin, and test method therefor Download PDFInfo
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- GB2612747A GB2612747A GB2302575.2A GB202302575A GB2612747A GB 2612747 A GB2612747 A GB 2612747A GB 202302575 A GB202302575 A GB 202302575A GB 2612747 A GB2612747 A GB 2612747A
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- 235000011852 gelatine desserts Nutrition 0.000 title claims abstract description 206
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- 238000010998 test method Methods 0.000 title abstract description 3
- 238000000034 method Methods 0.000 claims abstract description 11
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- 238000001514 detection method Methods 0.000 claims description 35
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- 150000002500 ions Chemical class 0.000 claims description 29
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- WROMPOXWARCANT-UHFFFAOYSA-N tfa trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F.OC(=O)C(F)(F)F WROMPOXWARCANT-UHFFFAOYSA-N 0.000 claims 1
- 238000002474 experimental method Methods 0.000 abstract description 3
- 238000012216 screening Methods 0.000 abstract description 3
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- HVIBGVJOBJJPFB-OFQRNFBNSA-N Gly-Pro-Hyp Chemical compound NCC(=O)N1CCC[C@H]1C(=O)N1[C@H](C(O)=O)C(O)CC1 HVIBGVJOBJJPFB-OFQRNFBNSA-N 0.000 description 3
- SNNYHIFMUVVACL-ASHKBJFXSA-N (2s)-2-[[2-[[(2s,3s)-2-[[(2s)-1-(2-aminoacetyl)pyrrolidine-2-carbonyl]amino]-3-methylpentanoyl]amino]acetyl]amino]-3-hydroxypropanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)CNC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]1CCCN1C(=O)CN SNNYHIFMUVVACL-ASHKBJFXSA-N 0.000 description 2
- KPYXMALABCDPGN-HYOZMBHHSA-N (4s)-5-[[(2s)-6-amino-1-[[(2s,3s)-1-[[(2s)-1-[[(2s)-1-[[(2s)-1-[[(2s)-1-[[(2r)-1-[[2-[[2-[[(1s)-3-amino-1-carboxy-3-oxopropyl]amino]-2-oxoethyl]amino]-2-oxoethyl]amino]-1-oxo-3-sulfanylpropan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]amino]-1-oxopropan-2-yl]a Chemical compound NC(=O)C[C@@H](C(O)=O)NC(=O)CNC(=O)CNC(=O)[C@H](CS)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN)CC1=CC=C(O)C=C1 KPYXMALABCDPGN-HYOZMBHHSA-N 0.000 description 2
- QEDRTIXTEAXNMY-XVMARJQXSA-N 2-[[(2s,4r)-1-[(2s)-2-aminopropanoyl]-4-hydroxypyrrolidine-2-carbonyl]amino]acetic acid Chemical compound C[C@H](N)C(=O)N1C[C@H](O)C[C@H]1C(=O)NCC(O)=O QEDRTIXTEAXNMY-XVMARJQXSA-N 0.000 description 2
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- KGGYQPXQVMSTRG-UHFFFAOYSA-N Gly-Ala-Hyp Natural products [NH3+]CC(=O)NC(C)C(=O)N1CC(O)CC1C([O-])=O KGGYQPXQVMSTRG-UHFFFAOYSA-N 0.000 description 2
- BMWFDYIYBAFROD-WPRPVWTQSA-N Gly-Pro-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)CN BMWFDYIYBAFROD-WPRPVWTQSA-N 0.000 description 2
- OHUKZZYSJBKFRR-WHFBIAKZSA-N Gly-Ser-Asp Chemical compound [H]NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(O)=O OHUKZZYSJBKFRR-WHFBIAKZSA-N 0.000 description 2
- ZLCLYFGMKFCDCN-XPUUQOCRSA-N Gly-Ser-Val Chemical compound CC(C)[C@H](NC(=O)[C@H](CO)NC(=O)CN)C(O)=O ZLCLYFGMKFCDCN-XPUUQOCRSA-N 0.000 description 2
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- KZNQNBZMBZJQJO-UHFFFAOYSA-N N-glycyl-L-proline Natural products NCC(=O)N1CCCC1C(O)=O KZNQNBZMBZJQJO-UHFFFAOYSA-N 0.000 description 2
- 108010047857 aspartylglycine Proteins 0.000 description 2
- 229920001436 collagen Polymers 0.000 description 2
- 108010017349 glycyl-prolyl-hydroxyproline Proteins 0.000 description 2
- 108010082286 glycyl-seryl-alanine Proteins 0.000 description 2
- 108010050297 hydroxyprolyl-glycine Proteins 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 108010061238 threonyl-glycine Proteins 0.000 description 2
- WFDSWNXTPKLLOT-UHNVWZDZSA-N 2-[[(2s,4r)-4-hydroxypyrrolidin-1-ium-2-carbonyl]amino]acetate Chemical compound O[C@H]1CN[C@H](C(=O)NCC(O)=O)C1 WFDSWNXTPKLLOT-UHNVWZDZSA-N 0.000 description 1
- MQIGTEQXYCRLGK-BQBZGAKWSA-N Ala-Gly-Pro Chemical compound C[C@H](N)C(=O)NCC(=O)N1CCC[C@H]1C(O)=O MQIGTEQXYCRLGK-BQBZGAKWSA-N 0.000 description 1
- 101100328890 Arabidopsis thaliana COL3 gene Proteins 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000282985 Cervus Species 0.000 description 1
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- 108010022452 Collagen Type I Proteins 0.000 description 1
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- PYTZFYUXZZHOAD-WHFBIAKZSA-N Gly-Ala-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)CN PYTZFYUXZZHOAD-WHFBIAKZSA-N 0.000 description 1
- QSDKBRMVXSWAQE-BFHQHQDPSA-N Gly-Ala-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@H](C)NC(=O)CN QSDKBRMVXSWAQE-BFHQHQDPSA-N 0.000 description 1
- FUTAPPOITCCWTH-WHFBIAKZSA-N Gly-Asp-Asp Chemical compound [H]NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(O)=O)C(O)=O FUTAPPOITCCWTH-WHFBIAKZSA-N 0.000 description 1
- JBCLFWXMTIKCCB-VIFPVBQESA-N Gly-Phe Chemical compound NCC(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 JBCLFWXMTIKCCB-VIFPVBQESA-N 0.000 description 1
- IEGFSKKANYKBDU-QWHCGFSZSA-N Gly-Phe-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CC2=CC=CC=C2)NC(=O)CN)C(=O)O IEGFSKKANYKBDU-QWHCGFSZSA-N 0.000 description 1
- GLACUWHUYFBSPJ-FJXKBIBVSA-N Gly-Pro-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)CN GLACUWHUYFBSPJ-FJXKBIBVSA-N 0.000 description 1
- JBCLFWXMTIKCCB-UHFFFAOYSA-N H-Gly-Phe-OH Natural products NCC(=O)NC(C(O)=O)CC1=CC=CC=C1 JBCLFWXMTIKCCB-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- CLNJSLSHKJECME-BQBZGAKWSA-N Pro-Gly-Ala Chemical compound OC(=O)[C@H](C)NC(=O)CNC(=O)[C@@H]1CCCN1 CLNJSLSHKJECME-BQBZGAKWSA-N 0.000 description 1
- CELJCNRXKZPTCX-XPUUQOCRSA-N Val-Gly-Ala Chemical compound CC(C)[C@H](N)C(=O)NCC(=O)N[C@@H](C)C(O)=O CELJCNRXKZPTCX-XPUUQOCRSA-N 0.000 description 1
- 108010047495 alanylglycine Proteins 0.000 description 1
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- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
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- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 108010081551 glycylphenylalanine Proteins 0.000 description 1
- 239000012567 medical material Substances 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
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- 235000004252 protein component Nutrition 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
- G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
A characteristic peptide segment capable of distinguishing a deer horn gelatin from a deer skin gelatin, and a test method therefor. By screening through a large number of experiments, a graph is drawn by using the proportion of a deer horn gelatin in a mixed gelatin as an abscissa and using values of Apeptide 1/Apeptide 3, and Apeptide 2/Apeptide 3 as ordinates, the proportion of the deer horn gelatin has a linear relationship to Apeptide 1/Apeptide 3, and a standard curve equation is established as: y=1.034x+0.0157, R2=0.9908; the proportion of the deer horn gelatin also has a linear relationship to Apeptide 2/Apeptide 3, and a standard curve equation is established as: y=0.7883x+0.0949, R 2=0.9905; the proportion of the deer horn gelatin doped is related to the values of Apeptide 1/Apeptide 3, and Apeptide 2/Apeptide 3, and a mixing proportion of the deer horn gelatin and a deer skin gelatin can be determined according to the ratios of Apeptide 1/Apeptide 3, and Apeptide 2/Apeptide 3. The method is strong in specificity, high in sensitivity and simple in operation, and is capable of distinguishing the deer horn gelatin from the deer skin gelatin, and controlling the quality.
Description
CHARACTERISTIC PEPTIDE SEGMENT CAPABLE OF DISTINGUISHING DEER HORN GELATIN FROM DEER SKIN GELATIN, AND TEST METHOD
THEREFOR
TECHNICAL FIELD
The present invention relates to a deer-derived specific peptide and a detection method therefor, in particular to a specific peptide for distinguishing a deer antler gelatin from a deer hide gelatin, as well as a sample adulterated with the deer hide gelatin in the deer antler gelatin and a proportion of the deer hide gelatin adulterated, and a detection method.
BACKGROUND
Gelatin medicinal materials comprise donkey-hide gelatin, deer hide gelatin, deer hide gelatin, cattle hide gelatin, and the like, and more than 80% of which are collagen of different types, comprising collagen type I alpha 1 chain (COL1A1), collagen type I alpha 2 chain (COL1A2), collagen type II alpha 1 chain (COL2A1), collagen type In alpha 1 chain CO (COL3 A 1), and the like, wherein the peptide fragments from the collagen type I alpha 1 (COL1) are prevailing. COL1, as a highly conservative protein, widely exists in different animal species, CO and is one of the important protein components of the gelatin medicinal material.
Both the deer antler gelatin and the deer hide gelatin are from Cervus nippon Temminck or Germs elaphus Linnaeuseerwes clap/ins Linnaeus, which are valuable Chinese medicinal materials. The deer antler gelatin is a gelatin stick made by decocting and concentrating Cervus nippon Temminck or Cenws elaphus Linnaeus antlers, while the deer hide gelatin is a gelatin stick made by decocting and concentrating dried skin or fresh skin of Cervus nippon Temminck or C'ervus elaphus Linnaeus. The antlers are ossified horns of Cenws elaphus Linnaeus or Cervus nippon Temminck, and the prices of which are much higher than that of the deer hide. In the market, there is a phenomenon of adulterating the deer hide gelatin into the deer antler gelatin. How to distinguish the deer antler gelatin from the deer hide gelatin is really a difficult problem in the identification research of the gelatin medicinal materials, which brings challenges to the identification of the deer antler gelatin and the deer hide gelatin, as well as the identification of the deer antler gelatin adulterated with the deer hide gelatin.
It is difficult to distinguish the deer antler gelatin from the deer hide gelatin in appearance, and both of the deer antler gelatin and the deer hide gelatin are from Cervu.s. nippon Temminck or Cervus elaphus Linnaeus, and have the same protein compositions. Therefore, it is basically impossible to distinguish the deer antler gelatin and the deer hide gelatin by searching for species-specific peptide fragments.
SUMMARY
Object of the present invention: the present invention determines ratios of relative contents of three deer-derived peptide fragments by screening through a large number of experiments, so as to be used for distinguishing a deer antler gelatin from a deer hide gelatin. The method is strong in specificity, high in sensitivity and simple in operation, and can be used for distinguishing the deer antler gelatin from the deer hide gelatin, and controlling the quality.
In order to achieve the above object, the present invention adopts the following technical solutions: A specific peptide capable of distinguishing a deer antler gelatin from a deer hide gelatin, wherein the characteristic peptide comprises: peptide 1: Gly-Ser-Asp-Gly-Ser-Val-Gly-Pro-Val-Gly-Pro-Ala-Gly-Pro-Ile-Gly-Ser-Ala-Gl y-ProHyp-Gly-Phe-Hyp-Gly-Ala-Hyp-Gly-Pro-Lys; CO peptide 2: Gly-Ser-Asp-Gly-Ser-Val-Gly-Pro-Val-Gly-Pro-Ala-Gly-Pro-Ile-Gly-Ser-Ala-Gl y-Pro-CO Hyp-Gly-Phe-Pro-Gly-Ala-Hyp-Gly-Pro-Lys; and peptide 3: Gly-Asp-Asp-Gly-Ala-Thr-Gly-Ala-Ala-Gly-Pro-Hyp-Gly-Pro-Thr-Gly-Pro-Ala-Gl y-Pro-Hyp-Gly-Phe-Pro-Gly-Ala-Val-Gly-Ala-Lys.
A detection method for a specific peptide capable of distinguishing a deer antler gelatin from a deer hide gelatin, comprises the following steps of (1) preparing the three deer-derived characteristic peptides according to claim 1 into a mixed control solution; and (2) subjecting deer hide gelatin and deer antler gelatin samples to be detected to enzyme digestion with trypsin, then injecting the enzymatic hydrolysate and the mixed control solution of the deer-derived characteristic peptides in the step (1) into a liquid chromatograph/mass spectrometer, taking the deer-derived characteristic peptides as control, and adopting an ESI positive ion mode and a multi-reaction monitoring mode for detection, wherein selected ion pairs comprise: peptide 1: m/z 864.0 (triple charge) ->535.4, peptide 2: m/z 858.7 (triple charge) ,527.4 and peptide 3: m/z 845.4 (triple charge) -> 507.4; and determining whether the sample is a deer hide gelatin or a deer antler gelatin by a ratio of a peak area Apeptide 1 of the peptide 1 to a peak area Apeptide 3 of the peptide 3 or a ratio of a peak area Aperido 2 of the peptide 2 to the peak area Apeptido 3 of the peptide 3.
As a preferred solution, in the detection method for the specific peptide capable of distinguishing the deer antler gelatin from the deer hide gelatin above, the enzyme digestion method comprises: adding 5 ml of phosphate buffer solution (pH = 6.0 to 8.5) into 10 mg of gelatin medicinal material sample to be detected, completely dissolving the sample by ultrasound, centrifuging the solution at 12,000 rpm for 20 minutes, placing 150 pl of supernatant into a 2 ml centrifuge tube, diluting the supernatant with 1 ml of 50 mM PBS, adding a proper amount of trypsin, shaking the mixture evenly for full enzymolysis, adding 60 pi of 10% v/v trifluoroacetic acid solution to stop the reaction, centrifuging the solution at 12,000 rpm for 20 minutes to obtain the enzymatic hydrolysate of the gelatin medicinal material, and placing the enzymatic hydrolysate at -20°C for storage and later use.
As a preferred solution, in the detection method for the specific peptide capable of distinguishing the deer antler gelatin from the deer hide gelatin above, a mass concentration of the trypsin added ranges from 0.1% to 10%.
As a preferred solution, in the detection method for the specific peptide capable of CO distinguishing the deer antler gelatin from the deer hide gelatin above, the enzymolysis method comprises: any one or a combination of more of constant-temperature enzymolysis at 37°C, CO microwave-assisted enzymolysis under 500 W to 1,000 W, ultrasonic-assisted enzymolysis under 20 kHz to 100 kHz and enzyme-immobilized enzymolysis.
As a preferred solution, in the detection method for the specific peptide capable of distinguishing the deer antler gelatin from the deer hide gelatin above, liquid phase conditions for detection by the liquid chromatograph/mass spectrometer are as follows: a chromatographic column is a L7 pm Waters C18 column with a specification of 2.1 pm x 100 mm, a sample size of 2 pl and a flow rate of 0.3 ml/min; 10% to 30% A linear gradient elution lasts for 0 to 3.5 minutes, 30% to 10% A linear gradient elution lasts for 3.5 minutes to 4 minutes, and 10% A linear gradient elution lasts for 4 minutes to 6 minutes; and a triple quadrupole mass spectrometry is used, and a mass spectrometry condition is: m/z 864.0 (triple charge) ->535.4, m/z 858.7 (triple charge) ->527.4 and m/z 845.4 (triple charge) ->507.4.
Apeptide 1/Apeptide 3 of the pure deer antler gelatin is no lower than 1.0, while Apeptide 2/AIN:Wide 3 of the pure deer antler gelatin is no lower than 0.5; Apeptide 1/Ap0Wide 3 of the pure deer hide gelatin is no higher than 0.2, while Apeptide 2/Apeptide 3 of the pure deer hide gelatin is no higher than 0.5. More preferably, Apeptide 1/Apeptide 3 of the pure deer antler gelatin is no lower than 1.3, while Apeptide 2/Apeptide 3 of the pure deer antler gelatin is no lower than 0.7; Apepticie 1/Apeptide 3 of the pure deer hide gelatin is no higher than 0.08, while Apeptide 2/Ap0ptide 3 of the pure deer hide gelatin is no higher than 0.19.
As a preferred solution, in the detection method for the specific peptide capable of distinguishing the deer antler gelatin from the deer hide gelatin above, a standard curve equation for detecting proportions of the deer hide gelatin and the deer antler gelatin is established as follows: mixing the deer antler gelatin and the deer hide gelatin according to proportions of 0%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100% respectively, adding 5 ml of phosphate buffer solution into 10 mg of mixed gelatin sample in each batch, completely dissolving the sample by ultrasound, centrifuging the solution at 12,000 rpm for 20 minutes, placing 150 jil of supernatant into a 2 ml centrifuge tube, diluting the supernatant with 1 ml of 50 mM PBS, adding trypsin with a mass concentration of 1%, shaking the mixture evenly for microwave enzymolysis for 30 minutes, adding 60 pi of 10% v/v trifluoroacetic acid solution to stop the reaction after enzymolysis, centrifuging the solution at 12,000 rpm for 20 minutes to obtain enzymolysis solutions of mixed gelatin samples with different proportions, and placing the enzymolysis solutions at -20°C for storage and later use; Cle) injecting the enzymatic hydrolysates of the mixed gelatin samples with different proportions into the liquid chromatograph/mass spectrometer, wherein a sample size is 1 jig, ("") and liquid phase conditions for detection by the liquid chromatograph/mass spectrometer are as follows: a chromatographic column is a 1.7 pm Cis reversed phase column with a specification of 2.1 tun 100 mm and a flow rate of 0.3 ml/min, a mobile phase A is acetonitrile, a mobile phase B is 0.1% formic acid, 10% to 30% A linear gradient elution lasts for 0 to 3.5 minutes, 30% to 10% A linear gradient elution lasts for 3.5 minutes to 4 minutes, and 10% A elution lasts for 4 minutes to 6 minutes; and a mass spectrometry condition for detection by the liquid chromatograph/mass spectrometer is: an electrospray positive ion mode _BSI+, and mass spectrometry parameters comprise: an ion source temperature of 500°C; an ionization voltage of 5,500 V; a desolvent temperature of 500°C; an ion source gas 1 of 60 psi; and an ion source gas 2 of 60 psi; setting the ion pair conditions corresponding to the characteristic peptides as follows: peptide 1: m/z 864.0 (triple charge) ->535.4, DP=166.57, CE=33.95; peptide 2: m/z 858.7 (triple charge) ->527.4, DP=160.35, CE=35.65; and peptide 3: m/z 845.4 (triple charge) ->507.4, DP=143.27, CE=31.50; and drawing a graph by using a proportion of the deer antler gelatin in the mixed gelatin as an abscissa and using values of Apeptide 1/Apepticie 3 and Ap epfide 2/Apepfide 3 as an ordinate, and since the proportion of the deer antler gelatin has a linear relationship to Ap cptide 1/Apcpfide 3, establishing the standard curve equation as: y=1.034x+0.0157, and R2=0.9908; since the proportion of the deer antler gelatin also has a linear relationship to ApepUde 2/Apeptide 3, establishing the standard curve equation as: y=0.7883x+0.0949, and R2=0.9905; and the proportion of the deer antler gelatin adulterated being related to the values of Apeptide 1/Apepticle 3 and Apeptido 2/Apeptide 3, and determining a mixing proportion of the deer antler gelatin and the deer hide gelatin according to ratios of Apeptide 1/Apeptid0 3 and Apoptido 2/Apeptide 3.
A detection kit for identifying a deer antler gelatin and a deer hide gelatin, wherein the kit comprises the three specific peptides according to claim 1. Certainly, other necessary reagents for liquid quality detection may also be comprised according to the need.
According to the method and the kit of the present invention, whether the sample to be detected is pure deer antler gelatin or pure deer hide gelatin can be determined, and the proportion of the deer hide gelatin to the deer antler gelatin in the mixed gelatin can also be determined.
Beneficial effects: compared with the prior art, the present invention has the following advantages.
CO The present invention determines ratios of relative contents of three deer-derived peptide fragments by screening through a large number of experiments, and establishes the linear CO equation for distinguishing the deer antler gelatin from the deer hide gelatin. The method is strong in specificity, high in sensitivity and simple in operation, and can be used for distinguishing the deer antler gelatin from the deer hide gelatin, and controlling the quality. The present invention can overcome the shortcomings of the prior art, such as the difficulty in distinguishing the deer antler gelatin from the deer hide gelatin in appearance and the difficulty in distinguishing specific peptide fragments, and has made very good technical progress.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG 1 shows a numerical relationship between a proportion of a deer antler gelatin in a mixed gelatin and Apeptide 1/Apeptid0 3 and Apeptidc 2/Apeptide 3.
FIG 2 is a mass spectrogram of the peptide I. FIG 3 is a mass spectrogram of the peptide 2 FIG 4 is a mass spectrogram of the peptide 3
DETAILED DESCRIPTION
The present invention will be further described in detail hereinafter with reference to the specific embodiments, but the present invention is not limited to these embodiments.
The trypsin used in the following embodiments was purchased from Promega Company. Embodiment 1 A deer-derived characteristic peptide had three characteristic peptide sequences, as shown in sequence table 1: peptide 1: Gly-Ser-Asp-Gly-Ser-Val-Gly-Pro-Val-Gly-Pro-Ala-Gly-Pro-Ile-Gly-Ser-Ala-Gl y-ProHyp-Gly-Phe-Hyp-Gly-Ala-Hyp-Gly-Pro-Lys; peptide 2: Gly-Ser-Asp-Gly-Ser-Val-Gly-Pro-Val-Gly-Pro-Ala-Gly-Pro-Ile-Gly-Ser-Ala-Gl y-ProHyp-Gly-Phe-Pro-Gly-Ala-Hyp-Gly -Pro-Lys; and peptide 3: Gly-Asp-Asp-Gly-Ala-Thr-Gly-Ala-Ala-Gly-Pro-Hyp-Gly-Pro-Thr-Gly-Pro-Ala-Gl yPro-Hyp-Gly-Phe-Pro-Gly-Ala-Val-Gly-Ala-Lys.
The polypeptides above were prepared by Nanjing GenScript Biotech Corporation using a solid phase synthesis method.
CO
Embodiment 2 Determination of Apepikk i/Apcod, 3 and Apeptid, 2/Apep11de 3in deer hide gelatin CO 10 batches of commercially available deer hide gelatin samples were taken, with each batch of 10 mg, added with 5 ml of phosphate buffer solution (pH=7.8), the samples were completely dissolved by ultrasound, centrifuged at 12,000 rpm for 20 minutes, 150 pl of supernatant were placed into a 2 ml centrifuge tube, diluted withl ml of 50 mM PBS, added with 1wt% trypsin, shaken evenly, and enzymolyzed at a constant temperature of 37°C for 12 hours. After enzymolysis, 60 pl of 10% v/v TFA solution was added to stop the reaction, and then centrifuged at 12,000 rpm for 20 minutes to obtain the enzymatic hydrolysate of the deer hide gelatin medical material, which was stored at -20°C for later use.
The enzymatic hydrolysates of the 10 batches of deer hide gelatin were injected into the liquid chromatograph/mass spectrometer, wherein a sample size was 1 pg, and liquid phase conditions for detection by the liquid chromatograph/mass spectrometer were as follows: a chromatographic column was a 1.7 pm CB reversed phase column (2.1 pm x 100 mm) with a flow rate of 0.3 ml/min, a mobile phase A was acetonitrile, a mobile phase B was 0.1% formic acid, 10% to 30% A linear gradient elution lasted for 0 to 3.5 minutes, 30% to 10% A linear gradient elution lasts for 3.5 minutes to 4 minutes, and 10% A elution lasts for 4 minutes to 6 minutes. A mass spectrometry condition for detection by the liquid chromatograph/mass spectrometer was: an electrospray positive ion mode ESI+, and mass spectrometry parameters
CO C
CO
comprised: an ion source temperature of 500°C; an ionization voltage of 5,500 V; a desolvent temperature of 500°C; an ion source gas 1 of 60 psi; and an ion source gas 2 of 60 psi. The ion pair conditions corresponding to the characteristic peptides were set as follows: peptide 1: m/z 864.0 (triple charge) ->535.4, DP=166.57, CE=33.95; peptide 2: m/z 858.7 (triple charge) ->527.4, DP=160.35, CE=35.65; and peptide 3: m/z 845.4 (triple charge) ->507.4, DP=143.27, CE=31.50.
The values of Apeptide 1/Apeptide 3 and Apeptide 2/Apeptide in the 10 batches of deer hide gelatin were shown in Table 1. The average value of Apeptide. /Apeptide 3 was 0.085+0.039, and the average value of Apeptide 2/Apeptid0 3 was 0.186+0.099.
Table 1 Results of Apeptide 1/Apeptide 3 and Apeptide 2/Apeptide in deer hide gelatin Batch Apeptide 1 Apeptide 2 Apeptide 3 Apeptide Average value Apeptide Average value 1/Apeptid0 3 of Apoptido 2/Apeptide 3 of Apoptido 1/Apeptide 3 2/Apeptide 3 Deer hide gelatin -1 40089 87160 558422 0.072 0.073+0.004 0.156 0.158+0.015 Deer hide gelatin -2 31266 69529 419380 0.075 0.166 Deer hide gelatin -3 42541 89309 638921 0.067 0.140 Deer hide gelatin -4 36495 69358 453431 0.080 0.153 Deer hide gelatin -5 45636 85574 633378 0.072 0.135 Deer hide gelatin -6 36678 87428 526985 0.070 0.166 Deer hide gelatin -7 35757 80563 505465 0.071 0.159 Deer hide gelatin -8 37183 77130 510655 0.073 0.151 Deer hide gelatin -9 33645 71669 423081 0.080 0.169 Deer hide gelatin -10 35380 86843 470534 0.075 0.185 Embodiment 3 Determination of Apeptide 1/Apeptide 3 and Arco& 2/Ap0ptid0 3 in deer antler gelatin batches of deer antler samples were taken and prepared into deer antler gelatin samples according to the method of preparing deer antler gelatin in Chinese Pharmacopoeia 2020, with each batch of 10 mg, added with 5 ml of phosphate buffer solution (pH=7.8), the samples were completely dissolved by ultrasound, centrifuged at 12,000 rpm for 20 minutes, 150 pl of supernatant were placed into a 2 ml centrifuge tube, diluted withl ml of 50 mM PBS, added with lwt()/0 trypsin, shaken evenly, and enzymolyzed by ultrasound for 10 minutes. After enzymolysis, 60 RI of 10% v/v TFA solution was added to stop the reaction, and then centrifuged at 12,000 rpm for 20 minutes to obtain the enzymatic hydrolysate of the deer antler gelatin, which was stored at -20°C for later use.
The enzymatic hydrolysates of the 10 batches of deer antler gelatin were injected into the liquid chromatograph/mass spectrometer, wherein a sample size was 1 jig, and liquid phase conditions for detection by the liquid chromatograph/mass spectrometer were as follows: a chromatographic column was a 1.7 pm Cis reversed phase column (2.1 pm >< 100 mm) with a CO flow rate of 0.3 ml/min, a mobile phase A was acetonitrile, a mobile phase B was 0.1% formic acid, 10% to 30% A linear gradient elution lasted for 0 to 3.5 minutes, 30% to 10% A linear CO gradient elution lasted for 3.5 minutes to 4 minutes, and 10% A elution lasted for 4 minutes to 6 minutes. A mass spectrometry condition for detection by the liquid chromatograph/mass spectrometer was: an electrospray positive ion mode ESI+, and mass spectrometry parameters comprised: an ion source temperature of 500°C; an ionization voltage of 5,500 V; a desolvent temperature of 500°C; an ion source gas 1 of 60 psi; and an ion source gas 2 of 60 psi. The ion pair conditions corresponding to the characteristic peptides were set as follows: peptide 1: m/z 864.0 (triple charge) ->535.4, DP=166.57, CE=33.95; peptide 2: m/z 858.7 (triple charge) ->527.4, DP=160.35, CE=35.65; and peptide 3: m/z 845.4 (triple charge) ->507.4, DP=143.27, CE=31.50.
The values of Apeptide 1/Apoptide 3 and Apeptide 2/Apeptide 3 in the 10 batches of deer antler gelatin were shown in Table 2. The average value of Apeptide 1 /Apeptide 3 was 1.659+0.453, and the average value of Apeptide 2/Ap0ptide 3 was 0.898+0.167.
Table 2 Results of Apoptide 1/Apeptide 3 and Apeptide 2/Apeptide 3 in deer antler gelatin Batch Apeptide 1 Apeptide 2 Apeptide 3 Apepti do Average value Apoptide Average value 1/Apeptide 3 of Apoptide 2/Apeptide 3 of Apoptide 1/Apoptide 3 2/Apeptide 3 Deer antler 790864 440075 522136 1.515 1.48310.060 0.843 0.81810.058
CO C
CO
gelatin -1 Deer antler gelatin -2 765946 398707 515180 1.487 0.774 Deer antler gelatin -3 736257 425948 517082 1.424 0.824 Deer antler gelatin -4 777514 437876 548103 1.419 0.799 Deer antler gelatin -5 896891 515919 612247 1.465 0.843 Deer antler gelatin -6 877082 434586 584903 1.500 0.743 Deer antler gelatin -7 852425 490406 561812 1.517 0.873 Deer antler gelatin -8 862729 489120 540670 1.596 0.905 Deer antler gelatin -9 820053 427177 589575 1 391 0.725 Deer antler gelatin -10 867846 490927 572470 1.516 0.858 Embodiment 4 Determination of Apepiule 1 /Apepluie 3 and Apeptule 2/Apeptule in mixed samples of deer hide gelatin and deer antler gelatin with different proportions The deer antler gelatin and the deer hide gelatin were mixed according to proportions of 0%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100%, added with 5 ml of phosphate buffer solution (p1-1=7.8) into 10 mg of mixed gelatin sample in each batch, the sample was completely dissolved by ultrasound, centrifuged at 12,000 rpm for 20 minutes, 150 pl of supernatant was placed into a 2 ml centrifuge tube, diluted with 1 ml of 50 mM PBS, added with I wt% trypsin, shaken evenly for microwave enzymolysis for 30 minutes, added with 60 pl of 10% v/v TFA solution to stop the reaction after enzymolysis, centrifuged at 12,000 rpm for 20 minutes to obtain enzymolysis solutions of mixed gelatin samples with different proportions, which were stored at -20°C for later use.
The enzymatic hydrolysates of the mixed gelatin samples with different proportions were injected into the liquid chromatograph/mass spectrometer in turn, wherein a sample size was 1 pg, and liquid phase conditions for detection by the liquid chromatograph/mass spectrometer are as follows: a chromatographic column was a 1.7 pm Cis reversed phase column (2.1 pm 100 mm) with a flow rate of 0.3 ml/min, a mobile phase A was acetonitrile, a mobile phase B was 0.1% formic acid, 10% to 30% A linear gradient elution lasted for 0 to 3.5 minutes, 30% to 10% A linear gradient elution lasted for 3.5 minutes to 4 minutes, and 10% A elution lasted for 4 minutes to 6 minutes.
A mass spectrometry condition for detection by the liquid chromatograph/mass spectrometer was: an electrospray positive ion mode ESI+, and mass spectrometry parameters comprised: an ion source temperature of 500°C; an ionization voltage of 5,500 V; a desolvent temperature of 500°C; an ion source gas 1 of 60 psi; and an ion source gas 2 of 60 psi. The ion pair conditions corresponding to the characteristic peptides were set as follows: peptide 1: m/z 864.0 (triple charge) ->535.4, DP=166.57, CE=33.95; peptide 2: m/z 858.7 (triple charge) ->527.4, DP=160.35, CE=35.65; and peptide 3: m/z 845.4 (triple charge) ->507.4, DP=143.27, CE=31.50.
Values of Apeptide 1/Apeptide 3 and Apeptide 2/Apeptide 3 of mixed gelatin samples with different proportions were shown in Table 3, and a numerical relationship between the proportion of the Cle) deer antler gelatin in the mixed gelatin and Apeptide 1/Apeptide 3 and Apeptide 2/Apeptide 3 was shown in FIG. 1. A graph was drawn by using the proportion of the deer antler gelatin in the mixed ("") gelatin as an abscissa and using the values of Apeptide 1/Apeptide 3 and Apeptide 2/Apeptide 3 as an
CD
ordinate, and the proportion of the deer antler gelatin had a linear relationship to Apeptide l/Apepiide 3, y=1.034x+0.0157, and R2=0.9908; the proportion of the deer antler gelatin also had a linear relationship to Apcppde 2/Apeptide 3, y=0.7883x+0.0949, and R2=0.9905. It was indicated that the proportion of the deer antler gelatin adulterated was related to the values of Apeptide 1/Apeptide 3 and Apeptide 2/Apeptide 3. Therefore, the mixing proportion of the deer antler gelatin and the deer hide gelatin could be determined according to the ratios of AIN:stick 1/Apeptide 3 and Apeptide 2/Apeptide 3.
Table 3 Relationship between the mixing proportion of the deer antler gelatin/deer hide gelatin mixed sample and the peak area Mixed gelain proportion Peak area Peak area ratio Proportion of Proportion of Peptide 1 Peptide 2 Peptide 3 Apoptido Apeptido deer antler deer hide 1/Apeptide 3 2/Apeptide 3 gelatin % gelatin % 0 100 50839 98330 798345 0.064 0.123 90 88348 144769 762672 0.116 0 190 80 151559 199047 778593 0.195 0.256
CO C
CO
70 215172 213294 692899 0.311 0.308 60 283652 278136 717316 0.395 0.388 50 393400 356366 775313 0.507 0.460 40 455462 357475 671879 0.678 0.532 30 449042 430880 624766 0.719 0.690 20 613801 512540 698997 0.878 0.733 10 623532 507367 635010 0.982 0.799 0 740081 657222 728625 1.016 0.902 Embodiment 5: Mixed control of the peptide 1, the peptide 2 and the peptide 3 with a certain concentration were taken, and fed for six times continuously under the above-mentioned chromatography -mass spectrometry conditions, to determine the peak areas of the control of the peptide 1, the peptide 2 and the peptide 3, and calculate the RSD of the peak areas of the control. The results were shown in Table 4, and the RSD of the peptide 1, the peptide 2 and the peptide 3 were respectively 2.00%, 0.83% and 2.70%, indicating that the method had excellent precision.
A corresponding concentration when a signal-to-noise ratio (S/N) of peptide 1, peptide 2 and peptide 3 was about 3, was taken as a limit of detection (LOD), and a corresponding concentration when the signal-to-noise ratio (S/N) of the peptide 1, the peptide 2 and the peptide 3 was about 10, was taken as a limit of quantitation (LOQ). The results were shown in Table 4, and the LOQ and the LOD of the peptide 1 were 0.62 ng/ml and 0.21 ng/ml respectively. The LOQ and the LOD of the peptide 2 were 0.67 ng/ml and 0.22 ng/ml respectively. The LOQ and the LOD of the peptide 3 were 0.58 ng/ml and 0.19 ng/ml respectively.
Table 4 Precision, limit of detection and limit of quantitation of peptide 1, peptide 2 and peptide 3 Precision (RSD, %) LOQ (ng/ml) LOD (ng/ml) Peptide I 2.00 0.62 0.21 Peptide 2 0.83 0.67 0.22 Peptide 3 2.70 0.58 0.19
SEQUENCE LISTING
<110> Nanjing University of Chinese Medicine <120> Specific peptide capable of distinguishing deer antler gelatin from deer hide gelatin and detection method therefor <130> <160>3 <170> PatentIn version 3.3 <210> 1 <211> 30 <212> PRT <213> Artificial sequence <400> 1 Gly Ser Asp Gly Ser Val Gly Pro Val Gly Pro Ala Gly Pro Ile Gly Ser Ala Gly Pro Hyp 1 5 10 15 20 CO Gly Phe Hyp Gly Ala Hyp Gly Pro Lys 30 CO <210>2 <211>30 <212> PRT <213> Artificial sequence <400> 2 Gly Ser Asp Gly Ser Val Gly Pro Val Gly Pro Ala Gly Pro Ile Gly Ser Ala 1 5 10 15 Gly Pro Hyp Gly Phe Pro Gly Ala Hyp Gly Pro Lys 25 30 <210>3 <211>30 <212> PRT <213> Artificial sequence <400> 3 Gly Asp Asp Gly Ala Thr Gly Ala Ala Gly Pro Hyp Gly Pro Thr Gly Pro Ala Gly Pro Hyp 1 5 10 15 20 Gly Plie Pro Gly Ala Val Gly Ala Lys 30
CO
C
CO
Claims (9)
- CLAIMS1. A specific peptide capable of distinguishing a deer antler gelatin from a deer hide gelatin, wherein the characteristic peptide comprises: peptide L Gly-Ser-Asp-Gly-Ser-Val-Gly-Pro-Val-Gly-Pro-Ala-Gly-Pro-Ile-Gly-Ser-Ala-Gl y-ProHyp-Gly-Phe-Hyp-Gly-Ala-Hyp-Gly-Pro-Lys; peptide 2: Gly-Ser-Asp-Gly-Ser-Val-Gly-Pro-Val-Gly-Pro-Ala-Gly-Pro-Ile-Gly-Ser-Ala-Gl y-ProHyp-Gly-Phe-Pro-Gly-Ala-Hyp-Gly-Pro-Lys; and peptide 3: Gly-Asp-Asp-Gly-Ala-Thr-Gly-Ala-Ala-Gly-Pro-Hyp-Gly-Pro-Thr-Gly-Pro-Ala-Gl yPro-Hyp-Gly-Phe-Pro-Gly-Ala-Val-Gly-Ala-Lys.
- 2. A detection method for a specific peptide capable of distinguishing a deer antler gelatin from a deer hide gelatin, comprising the following steps of: (1) preparing the three deer-derived specific peptides according to claim 1 into a mixed control solution; and (2) subjecting deer hide gelatin and deer antler gelatin samples to be detected to enzyme digestion with tiypsin, then injecting the enzymatic hydrolysate and the mixed control solution of the deer-derived characteristic peptides in the step (1) into a liquid chromatograph/mass spectrometer, taking the deer-derived characteristic peptides as control, and adopting an ESI positive ion mode and a multi-reaction monitoring mode for detection, wherein selected ion pairs comprise: peptide L m/z 864.0 (triple charge) ->535.4, peptide 2: m/z 858.7 (triple charge) ->527.4 and peptide 3: m/z 845.4 (triple charge) ->507.4; and determining whether the sample is a deer hide gelatin or a deer antler gelatin by a ratio of a peak area Apeptide 1 of the peptide 1 to a peak area Apeptide 3 of the peptide 3 or a ratio of a peak area Apepfide 2 of the peptide 2 to the peak area Apeptido 3 of the peptide 3.
- 3 The detection method for the specific peptide capable of distinguishing the deer antler gelatin from the deer hide gelatin according to claim 2, wherein the enzyme digestion method comprises: adding 5 ml of phosphate buffer solution (PBS) into 10 mg of gelatin medicinal material sample to be detected, completely dissolving the sample by ultrasound, centrifuging the solution at 12,000 rpm for 20 minutes, placing 150 pl of supernatant into a 2 ml centrifuge tube, diluting the supernatant with 1 ml of 50 mM PBS, adding a proper amount of trypsin, shaking the mixture evenly for full enzymolysis, adding 60 R1 of 10% trifluoroacetic acid (TFA) solution to stop the reaction, centrifuging the solution at 12,000 rpm for 20 minutes to obtain the enzymatic hydrolysate of the gelatin medicinal material, and placing the enzymatic hydrolysate at -20°C for storage and later use.
- 4. The detection method for the specific peptide capable of distinguishing the deer antler gelatin from the deer hide gelatin according to claim 3, wherein a mass concentration of the trypsin added ranges from 0.1% to 10%.
- 5. The detection method for the specific peptide capable of distinguishing the deer antler gelatin from the deer hide gelatin according to claim 3, wherein the enzymolysis method comprises: constant-temperature enzymolysis at 37°C, microwave-assisted enzymolysis, ultrasonic-assisted enzymolysis and enzyme-immobilized enzymolysis.
- 6. The detection method for the specific peptide capable of distinguishing the deer antler gelatin from the deer hide gelatin according to claim 3, wherein liquid phase conditions for detection by the liquid chromatograph/mass spectrometer are as follows: a chromatographic column is a 1.7 tan Waters Cis column with a specification of 2.1 im x 100 mm, a sample size of 2 [11 and a flow rate of 0.3 ml/min; 10% to 30% A linear gradient elution lasts for 0 to 3.5 minutes, 30% to 10% A linear gradient elution lasts for 3.5 minutes to 4 minutes, and 10% A linear gradient elution lasts for 4 minutes to 6 minutes; and a triple quadrupole mass spectrometry is used, and a mass spectrometry condition is: m/z 864.0 (triple charge) -535.4, m/z 858.7 (triple charge) ->527.4 and m/z 845.4 (triple charge) ->507.4.
- 7. The detection method for the specific peptide capable of distinguishing the deer antler gelatin from the deer hide gelatin according to claim 2, wherein Apeptide 1/Apoptide 3 of the deer antler gelatin is no lower than 1.0, while Apeptide 2/Apeptide 3 of the deer antler gelatin is no lower than 0.5; Apeplide 1 /ApepLide 3 of the deer hide gelatin is no higher than 0.2, while Apepiide 2/Apeptide 3 of the deer hide gelatin is no higher than 0.5.
- 8 The detection method for the specific peptide capable of distinguishing the deer antler gelatin from the deer hide gelatin according to claim 2, wherein a standard curve equation for detecting proportions of the deer hide gelatin and the deer antler gelatin is established as follows: mixing the deer antler gelatin and the deer hide gelatin according to proportions of 0%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100%, adding 5 ml of phosphate buffer solution into 10 mg of mixed gelatin sample in each batch, completely dissolving the sample by ultrasound, centrifuging the solution at 12,000 rpm for 20 minutes, placing 150 pl of supernatant into a 2 ml centrifuge tube, diluting the supernatant with 1 ml of 50 mM PBS, adding trypsin with a mass concentration of 1%, shaking the mixture evenly for microwave enzymolysis for 30 minutes, adding 60 pl of 10% trifluoroacetic acid solution to stop the reaction after enzymolysis, centrifuging the solution at 12,000 rpm for 20 minutes to obtain enzymolysis solutions of mixed gelatin samples with different proportions, and placing the enzymolysis solutions at -20°C for storage and later use; injecting the enzymatic hydrolysates of the mixed gelatin samples with different proportions into the liquid chromatograph/mass spectrometer, wherein a sample size is 1 jig, and liquid phase conditions for detection by the liquid chromatograph/mass spectrometer are as follows: a chromatographic column is a 1.7 Rm Cis reversed phase column with a specification of 2.1 pm 100 mm and a flow rate of 0.3 ml/min, a mobile phase A is acetonitrile, a mobile phase B is 0.1% formic acid, 10% to 30% A linear gradient elution lasts for 0 to 3.5 minutes, 30% to 10% A linear gradient elution lasts for 3.5 minutes to 4 minutes, and 10% A elution lasts for 4 minutes to 6 minutes; and a mass spectrometry condition for detection by the liquid chromatograph/mass spectrometer is: an electrospray positive ion mode ESI+, and mass spectrometry parameters comprise: an ion source temperature of 500°C; an ionization voltage of 5,500 V; a desolvent temperature of 500°C; an ion source gas 1 of 60 psi; and an ion source gas 2 of 60 psi; setting the ion pair conditions corresponding to the characteristic peptides as follows: peptide 1: m/z 864.0 (triple charge) ->535.4, DP=166.57, CE=33.95; peptide 2: m/z 858.7 (triple charge) ->527.4, DP=160.35, CE=35.65; and peptide 3: m/z 845.4 (triple charge) ->507.4, DP=143.27, CE=31.50; and drawing a graph by using a proportion of the deer antler gelatin in the mixed gelatin as an abscissa and using values of Apeptide 1/Apeptide 3 and Apeplide 2/Apeplide 3 as an ordinate, and since the proportion of the deer antler gelatin has a linear relationship to Apcptide 1/Apeptid0 3, establishing the standard curve equation as: y=1.034x+0.0157, and R2=0.9908; since the proportion of the deer antler gelatin also has a linear relationship to ApepLide 2/Apeptide 3, establishing the standard curve equation as: y=0.7883x+0.0949, and R2=0.9905; and the proportion of the deer antler gelatin adulterated being related to the values of Apeptide 1/Apeptide 3 and Apoptido 2/Ap0ptide 3, and determining a mixing proportion of the deer antler gelatin and the deer hide gelatin according to ratios of Apeptide 1/Apeptido and Apoptido 2/Apeptide 3
- 9. A detection kit for identifying a deer antler gelatin and a deer hide gelatin, wherein the kit comprises the three specific peptides according to claim I.
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| PCT/CN2020/131799 WO2022048049A1 (en) | 2020-09-01 | 2020-11-26 | Characteristic peptide segment capable of distinguishing deer horn gelatin from deer skin gelatin, and test method therefor |
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| CN112898384B (en) * | 2021-01-23 | 2022-08-26 | 南京中医药大学 | Deer horn characteristic peptide segment and detection method thereof |
| CN113173971B (en) * | 2021-04-20 | 2022-08-23 | 江阴天江药业有限公司 | Characteristic peptide segment for identifying formula granules containing deer horns or deer skins and detection method thereof |
| CN113173972B (en) * | 2021-04-20 | 2022-08-26 | 江阴天江药业有限公司 | Characteristic peptide segment for identifying deer horn or deer skin formula particles and detection method thereof |
| CN115248277B (en) * | 2022-06-27 | 2024-01-09 | 山东省食品药品检验研究院 | Cynanchum paniculatum characteristic polypeptide, application thereof and method for identifying Cynanchum paniculatum |
| CN116008409B (en) * | 2022-09-30 | 2023-08-11 | 山东省食品药品检验研究院 | Construction method and application of deer antler glue characteristic spectrum |
| CN115792243A (en) * | 2022-11-25 | 2023-03-14 | 北京同仁堂(辽宁)科技药业有限公司 | Method for detecting deer glue by using special ion pairs and application |
| CN116675761B (en) * | 2023-04-10 | 2024-02-27 | 山东省食品药品检验研究院 | Chorionic gonadotrophin characteristic polypeptide group and application thereof |
| CN117106031B (en) * | 2023-10-20 | 2024-01-02 | 山东省食品药品检验研究院 | Common characteristic peptide segment of reindeer horn, camel horn and deer horn and application thereof |
| CN117147881B (en) * | 2023-10-30 | 2024-01-02 | 山东省食品药品检验研究院 | Deer antler characteristic peptide fragment, method for detecting deer antler and application |
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| CN110824083B (en) * | 2019-11-06 | 2022-04-12 | 东阿阿胶股份有限公司 | Application of donkey-bone glue characteristic polypeptide in detection of donkey-bone glue components in animal skin glue and products thereof |
| CN111443134A (en) * | 2019-12-26 | 2020-07-24 | 北京化工大学 | Labeled peptide of sheep-derived component and application of labeled peptide in detection of gelatin and gelatin products |
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- 2020-09-01 CN CN202010905599.9A patent/CN112098578B/en active Active
- 2020-11-26 GB GB2302575.2A patent/GB2612747A/en active Pending
- 2020-11-26 WO PCT/CN2020/131799 patent/WO2022048049A1/en active Application Filing
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| US20030049715A1 (en) * | 2001-08-31 | 2003-03-13 | Welsch Dean J. | Peptide biomarker and method of identification |
| CN107532196A (en) * | 2015-03-30 | 2018-01-02 | 国立大学法人神户大学 | The authentication method and device of animal species |
| CN105273061A (en) * | 2015-10-09 | 2016-01-27 | 东阿阿胶股份有限公司 | Common polypeptide in animal glue and application thereof in detection |
| CN109187835A (en) * | 2018-09-17 | 2019-01-11 | 南京中医药大学 | A kind of discrimination method of the specificity peptide fragment containing protein-based Chinese medicine |
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| WO2022048049A1 (en) | 2022-03-10 |
| CN112098578A (en) | 2020-12-18 |
| GB202302575D0 (en) | 2023-04-12 |
| CN112098578B (en) | 2021-11-23 |
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