CN111443134A - Labeled peptide of sheep-derived component and application of labeled peptide in detection of gelatin and gelatin products - Google Patents
Labeled peptide of sheep-derived component and application of labeled peptide in detection of gelatin and gelatin products Download PDFInfo
- Publication number
- CN111443134A CN111443134A CN201911369177.8A CN201911369177A CN111443134A CN 111443134 A CN111443134 A CN 111443134A CN 201911369177 A CN201911369177 A CN 201911369177A CN 111443134 A CN111443134 A CN 111443134A
- Authority
- CN
- China
- Prior art keywords
- gelatin
- sheep
- gly
- ion
- pro
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229920000159 gelatin Polymers 0.000 title claims abstract description 71
- 235000019322 gelatine Nutrition 0.000 title claims abstract description 71
- 108010010803 Gelatin Proteins 0.000 title claims abstract description 69
- 235000011852 gelatine desserts Nutrition 0.000 title claims abstract description 69
- 239000008273 gelatin Substances 0.000 title claims abstract description 66
- 241001494479 Pecora Species 0.000 title claims abstract description 55
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 33
- 238000001514 detection method Methods 0.000 title claims abstract description 24
- 150000002500 ions Chemical class 0.000 claims description 28
- 241001465754 Metazoa Species 0.000 claims description 10
- 102000004142 Trypsin Human genes 0.000 claims description 10
- 108090000631 Trypsin Proteins 0.000 claims description 10
- 239000012588 trypsin Substances 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 8
- 239000004615 ingredient Substances 0.000 claims description 5
- 239000013558 reference substance Substances 0.000 claims description 5
- 238000001976 enzyme digestion Methods 0.000 claims description 4
- 235000013305 food Nutrition 0.000 claims description 4
- 230000014759 maintenance of location Effects 0.000 claims description 4
- 210000000988 bone and bone Anatomy 0.000 claims description 3
- 238000012544 monitoring process Methods 0.000 claims description 3
- 239000002994 raw material Substances 0.000 claims description 3
- 235000004252 protein component Nutrition 0.000 claims description 2
- 238000012360 testing method Methods 0.000 claims description 2
- 239000001828 Gelatine Substances 0.000 claims 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 230000007062 hydrolysis Effects 0.000 claims 1
- 238000006460 hydrolysis reaction Methods 0.000 claims 1
- 239000000825 pharmaceutical preparation Substances 0.000 claims 1
- 229940127557 pharmaceutical product Drugs 0.000 claims 1
- 239000012071 phase Substances 0.000 description 20
- 241000283707 Capra Species 0.000 description 16
- 239000000047 product Substances 0.000 description 15
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 12
- 102000004196 processed proteins & peptides Human genes 0.000 description 12
- 239000003550 marker Substances 0.000 description 10
- 241000283690 Bos taurus Species 0.000 description 9
- 241000282898 Sus scrofa Species 0.000 description 9
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 8
- 229920001184 polypeptide Polymers 0.000 description 8
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 239000003814 drug Substances 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 4
- 238000010828 elution Methods 0.000 description 4
- 235000019253 formic acid Nutrition 0.000 description 4
- 239000007791 liquid phase Substances 0.000 description 4
- 238000004949 mass spectrometry Methods 0.000 description 4
- 108020004999 messenger RNA Proteins 0.000 description 4
- 235000018102 proteins Nutrition 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 description 3
- 229910000013 Ammonium bicarbonate Inorganic materials 0.000 description 3
- 241000283073 Equus caballus Species 0.000 description 3
- 235000012538 ammonium bicarbonate Nutrition 0.000 description 3
- 239000001099 ammonium carbonate Substances 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 238000007865 diluting Methods 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000000706 filtrate Substances 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 3
- 238000001819 mass spectrum Methods 0.000 description 3
- 239000012982 microporous membrane Substances 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000002791 soaking Methods 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 102000008186 Collagen Human genes 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- 101000978703 Escherichia virus Qbeta Maturation protein A2 Proteins 0.000 description 2
- 206010064571 Gene mutation Diseases 0.000 description 2
- 150000001413 amino acids Chemical group 0.000 description 2
- 229920001436 collagen Polymers 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 230000003301 hydrolyzing effect Effects 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 208000031295 Animal disease Diseases 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000033444 hydroxylation Effects 0.000 description 1
- 238000005805 hydroxylation reaction Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/72—Mass spectrometers
- G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
- G01N30/724—Nebulising, aerosol formation or ionisation
- G01N30/7266—Nebulising, aerosol formation or ionisation by electric field, e.g. electrospray
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/86—Signal analysis
- G01N30/8624—Detection of slopes or peaks; baseline correction
- G01N30/8631—Peaks
- G01N30/8637—Peak shape
Landscapes
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention relates to a labeled peptide of sheep-derived components and application thereof in detection of gelatin and gelatin products, belonging to the technical field of detection.
Description
Technical Field
The invention relates to a labeled peptide of sheep-derived components and application thereof in detection of gelatin and gelatin products, belonging to the field of medicine and food detection.
Background
The gelatin is prepared by properly hydrolyzing animal bones, skins, tendons, scales and the like serving as raw materials, has excellent physicochemical properties, and is widely applied to the fields of food, medicine and the like. The use of gelatin extracted from different animals is often different; more importantly, due to the potential safety risks, halal requirements and religious beliefs that animal diseases may pose, it is necessary to trace the source of the animal-derived components of gelatin. 95% of gelatin in the world comes from pigs, cattle or sheep, so that the method has important significance in tracing and detecting sheep-derived components.
The main component of the gelatin is collagen polypeptide, and the animal source component can be traced by detecting the polypeptide by using a liquid chromatograph-mass spectrometer, however, whether the selected polypeptide can be used as a tracing marker or not is related to the success of tracing because the polypeptide has the phenomena of mutation and the like in the same species. The sheep is divided into sheep and goat, and the selection of the marker peptide for tracing the sheep-derived ingredients needs to overcome the influence caused by gene mutation and also needs to be contained in the sheep and the goat together. The marker peptide which is not mutated in the sheep and is commonly contained in the sheep and the goat is selected to become the key of tracing sheep-derived ingredients. The above difficulties cannot be overcome by directly adopting a liquid chromatograph-mass spectrometer to detect and analyze the sample. Starting from genes, analyzing and searching for marker peptides is a feasible path.
Disclosure of Invention
Aiming at the problems, the invention provides the labeled peptide of the sheep-derived component and the detection method of the sheep-derived component in the gelatin and the gelatin products, the method has simple operation, strong characteristics and high sensitivity, and can be used for the traceability identification and content determination of the sheep-derived component in the gelatin and the gelatin products.
The technical scheme of the invention is as follows:
a marker peptide of sheep origin component and its application in gelatin and gelatin product detection, contain the marker peptide in the collagen sequence based on goat and sheep, its amino acid sequence is Gly-Pro-Ala-Gly-Pro-Thr-Gly-Pro-Ala-Gly-L ys. it is from the mRNA of CO L1A 2 protein in horse, cattle, pig, sheep, find out the characteristic mRNA sequence that sheep exists steadily, utilize the liquid chromatography-mass spectrometry to detect after translating into the protein sequence, the marker peptide selected finally.
(1) Performing enzyme digestion on a sample to be detected by using trypsin, or extracting protein components in the sample to be detected and performing enzyme digestion by using trypsin;
(2) putting into a liquid chromatography-mass spectrometer, and monitoring by selecting parent ion m/z455.5 and daughter ion thereof with labeled peptide or gelatin of sheep-derived component as reference; if the retention time of the detected ion is consistent with that of a reference substance and the daughter ion of the detected ion is consistent with that of the reference substance, the gelatin or the product thereof contains sheep-derived components, and the content of the sheep-derived components can be calculated by peak areas of the reference substance and the detected sample; if there is no such ion that is consistent with the retention time of the control, then no ovine derived component is present.
The gelatin is prepared by hydrolyzing animal bone, skin and scale as raw materials, and the gelatin product is food, health product or medicine containing gelatin.
By adopting the method, whether the sheep-derived components are contained in the gelatin and the gelatin product can be rapidly detected. Although the gene mutation in animals causes protein sequence difference and protein sequence difference in different sheep and goats, the gene of the marker peptide has strong conservation in sheep and goats and is shared by sheep and goats, and the detection of the marker peptide can trace the source of sheep-derived ingredients in gelatin and gelatin products.
Drawings
FIG. 1 is a scanning mass spectrum of a daughter ion of a labeled peptide in goat gelatin and sheep gelatin (parent ion m/z455.5, daughter ion scanning range m/z 200-1100);
FIG. 2 is a mass spectrum of selected ion pairs m/z455.5 → 628.7, 685.4, 756.5 monitoring under various animal gelatin SRM scanning modes;
FIG. 3 is a graph relating the mass spectrometric peak detection areas (m/z455.5 → 685.4) of labeled peptides corresponding to different contents of sheep gelatin;
FIG. 4 shows the mass spectra of sheep gelatin and gelatin preparations (QQ saccharide as an example) monitored in SRM scanning mode for selected ion pairs m/z455.5 → 628.7, 685.4, 756.5.
Detailed Description
The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. The examples are illustrative only and do not limit the scope of the present invention in any way. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.
Example 1
1 materials and reagents
Materials: the gelatin derived from different animals is respectively extracted from sheep, goat, cattle, pig and horse skin.
Reagent: ammonium bicarbonate (analytically pure), trypsin (sequence pure, purchased from the chinese institute for drug and biological product assay).
2 detection method
(1) Preliminary screening of labeled peptides
Through a plurality of batches of mRNA comparison tests of various animal CO L A2 proteins, the first base A and T and the second base C and T are basically not mutated in the same species, but mutation among species is frequently occurred, among three bases corresponding to each amino acid, the mRNA of the goat CO L A2 protein contains "GGC CCT GCT GGT CCT ACT GGC CCC GCT GGCAAA", in the corresponding position, sheep is "GGC CCC GCT GGT CCT ACT GGC CCC GCT GGC AAA", cattle is "GGC CCT GCT GGT CCT TCT GGC CCC GCT GGC AAA", pig is "GGC CCAGCT GGT CCT TCT GGCCCT GCT GGC AAA", horse is "GGC CCT GCT GGT CCT ACT GGC CCT GTC GGC AAA", after translation, the corresponding polypeptide, sheep and goat is "Gly-Pro-Ala-Gly-Pro-Thr-Gly-Pro-Ala-Gly-Pro-L ys", cow is "Gly-Pro-Ala-Gly-Pro-Ser-Gly-Pro-Ala-Gly-Pro-Gly-L ys", pig is "Gly-Pro-Ala-Gly-Pro-Ser-Gly-Pro-Ala-Gly-L ys", pig is "Gly-Pro-Ala-Gly-Pro-Ser-Gly-Pro-Ala-Gly-Pro-Ala-Gly-L ys", pig is "Gly-Pro-Ser-Gly-Pro-Ala-Gly-Pro-Ala-Ser-Gly-Ser-Pro-Gly-Pro-Gly-Pro-.
(2) Determination of labeled peptides
a) Taking 0.05g gelatin sample in a 25ml measuring flask, adding a small amount of 1% NH4HCO3Soaking in the solution for 10min, heating at 60 deg.C to dissolve, and dissolving with 1% NH4HCO3Diluting the solution to scale, shaking, filtering with microporous membrane, precisely taking 0.2ml of the subsequent filtrate, adding 20 μ l of 2mg/ml trypsin solution, and performing enzymolysis at 37 deg.C for 12 hr.
b) And (3) putting 5 mu l of goat and sheep gelatin enzymolysis solution into a liquid chromatography-mass spectrometer to detect whether the polypeptide is subjected to hydroxylation modification. Liquid phase conditions: c18Reversed phase chromatographic column (2.1mm × 100mm, 1.8 μm), mobile phase A0.1% formic acid solution, mobile phase B acetonitrile, flow rate 0.3ml/min, gradient elution 0-25 min, 98% → 80% mobile phase A, 2% → 20% mobile phase B, mass spectrometry conditions electrospray positive ion mode (ESI)+) And selecting m/z455.5 as the parent ion to perform the full scan of the daughter ion. The results are shown in figure 1 and indicate that polypeptide GPAGPTGPAGK, in which no proline is hydroxylated, is present in both goat and sheep gelatins.
c) And (3) putting 5 mu l of enzymolysis solution into a liquid chromatograph-mass spectrometer for detection. Liquid phase conditions: c18Reversed phase chromatographic column (2.1mm × 100mm, 1.8 μm), mobile phase A0.1% formic acid solution, mobile phase B acetonitrile, flow rate 0.3ml/min, gradient elution 0-25 min, 98% → 80% mobile phase A, 2% → 20% mobile phase B, mass spectrometry conditions electrospray positive ion mode (ESI)+) The SRM detection is selected, and m/z455.5 → 628.7, 685.4 and 756.5 are selected as detection ion pairs. The results are shown in FIG. 2, only the corresponding ion peaks were detected in goat and sheep gelatins at 4.8min, and none of the others were detected.
In conclusion, the polypeptide GPAGPTGPAGK can be used as a marker peptide of sheep derived components.
Example 2
1 materials and reagents
Materials: mixed gelatin samples (including 5% sheep gelatin-containing bovine gelatin, 10% sheep gelatin-containing bovine gelatin, 20% sheep gelatin-containing bovine gelatin, 40% sheep gelatin-containing bovine gelatin, pure sheep gelatin) were prepared by precisely mixing the gelatins of example 1.
Reagent: ammonium bicarbonate (analytically pure), trypsin (sequence pure, purchased from the chinese institute for drug and biological product assay).
2 detection method
(1) Taking 0.05g gelatin sample in a 25ml measuring flask, adding a small amount of 1% NH4HCO3Soaking in the solution for 10min, heating at 60 deg.C to dissolve, and dissolving with 1% NH4HCO3Diluting the solution to scale, shaking, filtering with microporous membrane, precisely taking 0.2ml of the subsequent filtrate, adding 20 μ l of 2mg/ml trypsin solution, and performing enzymolysis at 37 deg.C for 12 hr.
(2) And (3) putting 5 mu l of enzymolysis solution into a liquid chromatograph-mass spectrometer for detection. Liquid phase conditions: c18Reversed phase chromatographic column (2.1mm × 100mm, 1.8 μm), mobile phase A0.1% formic acid solution, mobile phase B acetonitrile, flow rate 0.3ml/min, gradient elution 0-25 min, 98% → 80% mobile phase A, 2% → 20% mobile phase B, mass spectrometry conditions electrospray positive ion mode (ESI)+) The SRM detection is selected, and m/z455.5 → 628.7, 685.4 and 756.5 are selected as detection ion pairs.
The curve was plotted with the percentage of sheep gelatin as abscissa (X) and the peak area (m/z455.5 → 685.4) as ordinate (Y). The result is shown in FIG. 3, where the standard curve Y is 93780X +1303.7 and the linear correlation coefficient R2The linearity is good at 0.9938. Therefore, the method can be used for detecting the content of the sheep-derived components.
Example 3
1 materials and reagents
Materials: sheep gelatin, QQ candy added with pig gelatin (self-made), QQ candy added with sheep gelatin (self-made)
Reagent: ammonium bicarbonate (analytically pure), trypsin (sequence pure, purchased from the chinese institute for drug and biological product assay).
2 detection method
(1) Respectively placing 0.05-0.25 g of sheep gelatin, QQ sugar added with pig gelatin and QQ sugar sample added with sheep gelatin in a 25ml measuring flask, and adding a small amount of 1% NH4HCO3Soaking in the solution for 10min, heating at 60 deg.C to dissolve, and dissolving with 1% NH4HCO3Diluting the solution to scale, shaking, filtering with microporous membrane, precisely taking 0.2ml of the subsequent filtrate, adding 20 μ l of 2mg/ml trypsin solution, and performing enzymolysis at 37 deg.C for 12 hr.
(2) And (3) putting 5 mu l of enzymolysis solution into a liquid chromatograph-mass spectrometer for detection. Liquid phase conditions:C18reversed phase chromatographic column (2.1mm × 100mm, 1.8 μm), mobile phase A0.1% formic acid solution, mobile phase B acetonitrile, flow rate 0.3ml/min, gradient elution 0-25 min, 98% → 80% mobile phase A, 2% → 20% mobile phase B, mass spectrometry conditions electrospray positive ion mode (ESI)+) The SRM detection is selected, and m/z455.5 → 628.7, 685.4 and 756.5 are selected as detection ion pairs.
The results are shown in figure 4, and the corresponding ion peaks are detected in the goat gelatin and the QQ sugar added in the goat gelatin at 4.8min, and the QQ sugar added in the pig gelatin is not detected, so that the method can specifically detect the sheep-derived ingredients in the gelatin product.
In conclusion, the sheep-derived component labeled peptide disclosed by the invention can be used for accurately detecting sheep-derived components in gelatin and gelatin products through LC-MS (liquid chromatography-mass spectrometry) detection.
It should be noted that the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described by referring to the preferred embodiments of the present invention, various changes or modifications of the present invention by those skilled in the art are made without departing from the spirit and scope of the present invention, and these equivalents also fall within the scope of the present invention.
Claims (5)
1. A labeled peptide of sheep derived component has amino acid sequence of Gly-Pro-Ala-Gly-Pro-Thr-Gly-Pro-Ala-Gly-L ys.
2. Use of the labeled peptide according to claim 1 in the detection of gelatin and gelatin products, comprising the steps of:
(1) performing enzyme digestion on a sample to be detected by using trypsin, or extracting protein components in the sample to be detected and performing enzyme digestion by using trypsin;
(2) putting into a liquid chromatograph-mass spectrometer, and selecting parent ion m/z455.5 and daughter ions thereof for monitoring by using gelatin for marking peptide or sheep-derived components as reference; if the retention time of the ion is consistent with that of the reference substance and the daughter ion of the ion is consistent with that of the reference substance, the gelatin or the product thereof contains sheep-derived ingredients; if there is no such ion that is consistent with the retention time of the control, then no ovine derived component is present.
3. The use according to claim 2, wherein the ovine component content is calculated from the peak areas of the control and the test sample.
4. Use according to claim 2, wherein the gelatin is obtained by hydrolysis of animal bones, skins and scales.
5. Use according to claim 2, wherein the gelatine product is a food, health product or pharmaceutical product comprising gelatine in the raw material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911369177.8A CN111443134A (en) | 2019-12-26 | 2019-12-26 | Labeled peptide of sheep-derived component and application of labeled peptide in detection of gelatin and gelatin products |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911369177.8A CN111443134A (en) | 2019-12-26 | 2019-12-26 | Labeled peptide of sheep-derived component and application of labeled peptide in detection of gelatin and gelatin products |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111443134A true CN111443134A (en) | 2020-07-24 |
Family
ID=71650643
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911369177.8A Pending CN111443134A (en) | 2019-12-26 | 2019-12-26 | Labeled peptide of sheep-derived component and application of labeled peptide in detection of gelatin and gelatin products |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111443134A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112098578A (en) * | 2020-09-01 | 2020-12-18 | 南京中医药大学 | Characteristic peptide segment capable of distinguishing antler glue and deer skin glue and detection method thereof |
| CN112782291A (en) * | 2020-09-23 | 2021-05-11 | 山东省食品药品检验研究院 | Method for identifying donkey-derived components in donkey-hide gelatin and preparation thereof |
| CN113501861A (en) * | 2021-08-01 | 2021-10-15 | 青海瑞肽生物科技有限公司 | Method for screening characteristic fragments of yak collagen by high-resolution mass spectrometry |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106093244A (en) * | 2016-07-01 | 2016-11-09 | 山东省食品药品检验研究院 | A kind of sheep characteristic polypeptide and application thereof |
| CN110596283A (en) * | 2019-07-19 | 2019-12-20 | 深圳市计量质量检测研究院（国家高新技术计量站、国家数字电子产品质量监督检验中心） | Liquid chromatography-mass spectrometry method for identifying species attributes of leather products such as pigs, cattle and sheep |
-
2019
- 2019-12-26 CN CN201911369177.8A patent/CN111443134A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106093244A (en) * | 2016-07-01 | 2016-11-09 | 山东省食品药品检验研究院 | A kind of sheep characteristic polypeptide and application thereof |
| CN110596283A (en) * | 2019-07-19 | 2019-12-20 | 深圳市计量质量检测研究院（国家高新技术计量站、国家数字电子产品质量监督检验中心） | Liquid chromatography-mass spectrometry method for identifying species attributes of leather products such as pigs, cattle and sheep |
Non-Patent Citations (2)
| Title |
|---|
| 张玲玲 等: "INH表位多肽疫苗抗体间接ELISA测定方法", 《中国畜牧兽医》 * |
| 阳洪波 等: "基于特征肽段的液相色谱－质谱技术鉴定胶原蛋白的物种来源", 《分析测试学报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112098578A (en) * | 2020-09-01 | 2020-12-18 | 南京中医药大学 | Characteristic peptide segment capable of distinguishing antler glue and deer skin glue and detection method thereof |
| CN112098578B (en) * | 2020-09-01 | 2021-11-23 | 南京中医药大学 | Characteristic peptide segment capable of distinguishing antler glue and deer skin glue and detection method thereof |
| CN112782291A (en) * | 2020-09-23 | 2021-05-11 | 山东省食品药品检验研究院 | Method for identifying donkey-derived components in donkey-hide gelatin and preparation thereof |
| CN113501861A (en) * | 2021-08-01 | 2021-10-15 | 青海瑞肽生物科技有限公司 | Method for screening characteristic fragments of yak collagen by high-resolution mass spectrometry |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Sarah et al. | LC–QTOF-MS identification of porcine-specific peptide in heat treated pork identifies candidate markers for meat species determination | |
| CN111443134A (en) | Labeled peptide of sheep-derived component and application of labeled peptide in detection of gelatin and gelatin products | |
| CN113480599A (en) | Characteristic polypeptide for identifying deer antler glue of sika deer or red deer and application thereof | |
| US20220033873A1 (en) | Quantitative detection method for snake venom thrombin-like enzyme (svtle) | |
| Gu et al. | Identification of peptide biomarkers for authentication of Atlantic salmon and rainbow trout with untargeted and targeted proteomics approaches and quantitative detection of adulteration | |
| CN102435680B (en) | Bovine serum albumin non-labeled mass spectrum qualitative and quantitative detection method | |
| Guo et al. | A rapid and simple UPLC-MS/MS method using collagen marker peptides for identification of porcine gelatin | |
| CN109791131A (en) | LC/MS/MS analysis for the identification of the meat kind of raw meat and meat processing product | |
| CN106093244A (en) | A kind of sheep characteristic polypeptide and application thereof | |
| Fricker | Quantitative peptidomics: general considerations | |
| CN111766324A (en) | Characteristic peptide combination and method for detecting milk doped in buffalo milk | |
| CN108426970A (en) | The method that enzymolysis stability by investigating candidate peptide fragment filters out the feature peptide fragment of milk powder allergen protein | |
| CN114113381B (en) | Syngnathus schutz characteristic polypeptide, application thereof and method for identifying comfortable Syngnathus schutz | |
| Zhang et al. | Species-specific identification of donkey-hide gelatin and its adulterants using marker peptides | |
| CN106198790B (en) | A kind of horse and mule common characteristic polypeptide and its application | |
| EP1887351A1 (en) | Screening method for specific protein in proteome comprehensive analysis | |
| CN110678756B (en) | Method for absolute quantification of low abundance polypeptides using mass spectrometry | |
| CN106589114A (en) | Porcine-derived characteristic peptide and application processes thereof in pigskin and colla corii asini qualitative detection | |
| Meyer et al. | High‐performance proteomics as a tool in biomarker discovery | |
| CN111896663B (en) | Chicken-derived characteristic collagen peptide III and application thereof in detection of collagen hydrolysate and products thereof | |
| CN111060622B (en) | Labeled peptide of tuna-derived component and application of labeled peptide in detection of collagen degradation product and product thereof | |
| CN111735891A (en) | Poultry-derived characteristic collagen peptide and application thereof in detection of collagen hydrolysate and products thereof | |
| CN111896664B (en) | Tuna characteristic collagen peptide and application thereof in identification of collagen hydrolysate and products thereof | |
| CN111751476B (en) | Duck-derived characteristic III-type collagen peptide and application thereof in detection of collagen hydrolysate and products thereof | |
| CN113429474B (en) | Method for identifying adulteration of vegetable protein meat sample based on characteristic peptide fragment label |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200724 |
|
| RJ01 | Rejection of invention patent application after publication |