GB2518027A

GB2518027A - Chinese medicine composition for lowering lipid and protecting liver as well as preparation method and application thereof

Info

Publication number: GB2518027A
Application number: GB1408587.2A
Authority: GB
Inventors: Weiquan Li; Juan Tong; Dongxiao Li
Original assignee: Individual
Current assignee: Individual
Priority date: 2013-05-15
Filing date: 2014-05-14
Publication date: 2015-03-11
Anticipated expiration: 2034-05-14
Also published as: JP2014224113A; TW201505645A; CN103230444B; CN103230444A; GB201408587D0; HK1208169A1; GB2518027B; SG10201402234TA; KR20140135106A

Abstract

A Chinese medicine composition for lowering lipid levels and protecting the liver comprises 60 to 120 parts by weight of Gynura divaricata, 50 to 110 parts by weight of Radix Puerariae, and 20 to 60 parts by weight of Radix Paeoniae Alba. The Gynura divaricata is preferably the whole herb of Gynura divaricata, the Radix Puerariae is preferably the dried root of Pueraria lobata and the Radix Paeoniae is preferably the dried root of Paeonia lactiflora. A method of preparing the composition involving decoction of each of Gynura divaricata, Radix Puerariae, and Radix Paeoniae Alba is described. The composition is useful in the treatment and prevention of hyperlipidemia, obesity, viral hepatitis, chemical liver injury and alcoholic liver injury.

Description

Chinese medicine composition for lowering lipid and protecting liver as well as preparation method and application thereof

TEChNICAL FIELD

The present invention relates to the field of Chinese medicine composition, particularly to a Chinese medicine composition for lowering lipid and protecting liver as well as preparation method and application thereof.

BACKGROUND

Liver injuly comprises viral liver injury and chemical liver injury. The viral liver injury is the liver inflammation infected by pathogenic microorganism such as virus, e.g. hepatitis A, hepatitis 13. The ehcinical liver injury is caused by various toxicants, such as alcohol in foods, chemical toxicarit in the environment, or medicines. Viral hepatitis is a.n infectious disease spreading all over the world, hundreds of millions of patients suffer from hepatitis every year.

and about two million people died from it. Major chemical liver injury (including alcoholic liver injury) comprise steatosis, lipid peroxidation which is a special form of toxic liver injury, and cholestasis, mainly relating to dysfunction of bile acid excretion caused by inj cry of liver cell membrane and in icrovi Ill. Such two kinds of liver injuries are one of diseascs damaging physical and psychological health severely.

With improvement of living standard, people's dietary pattern and life style has changed greatly. Experts predict that population of obesity will be more than 200 millions, morbidities of hyperlipidemia and fatty liver will rise accordingly. Currently, the population of patients suffering hyperlipidemia is up to 90 millions.

it is significant to research on medicines and health products for preventing and treating hepatitis as well as hyperlipideniia, which are suitable for long-term use, by using resources of traditional Chinese medicine and modern teclrnology of biology and preparation.

SUMMARY OF THE INVENTION

The object of the present invention is to provide a Chinese medicine composition for lowering lipid and protecting liver, having a thuction of preventing and treating hepatitis as well as hyperlipidemia.

Another object of the present invention is to provide a preparation method of aforesaid Chinese medicine composition for lowering lipid and protecting liver.

Further object of the present invention is to provide application of aforesaid Chinese medicine composition for lowering lipid and protecting liver on preparing medicines or health prodncts used for preventing and treating hyperlipidemia, obesity; viral hepatitis, chemical liver ifljLL1, and alcoholic liver injury.

The objects of the present invention can he achieved by a Chinese medicine composition for lowering lipid and protecting liver, which is the compound preparation formed by Chinese medicine components and pharmaceutical adjuvants, comprising 60 to 120 parts by weight of Gynura divaricata, 50 to 110 parts by weight of Radix Puerariae and 20 to 60 parts by weight of Radix Paeoniae Alba.

Preferably, Gynura divaricata is 80 to 100 parts by weight, Radix Puerariae is 70 to 90 parts by weight, and Radix Paeoniae Alba is 30 to 50 parts by weight.

The Gynura divaricata is the whole herb of Gynura divaricata.

The Radix Puerariae is the dried root of Pueraria lobata.

The Radix l'aeoniae Mba is the dried root of Faeonia lactiflora.

The compound preparation is in form of capsules, pills, granules, or oral liquid.

The pharmaceutical adjuvant is one of, or a mixture of two or more of dextrin, saccharose, lactose, starch, sodium carboxyrnethyl starch, polyvinylpyrrolidone, and magnesium stearate.

The preparation method of thc aforesaid Chinese medicine composition compriscs steps as follows: 1) decocting Gynura divaricata with water for 1.5 to 2 hours, wherein the weight of water is 8 to 12 times the weight of Gynura divaricata, collecting the extracting solution; then adding water again into the residue thereof, wherein the weighi of water is 5 to 8 times the weight of the residue, dccocting them for 1 to 1.5 hours, collecting the extracting solution again; mixing both extracting solutions, filtrating and concentrating it, then adding alcohol thereto and making the content of alcohol in 70% to 90%, filtrating, concentrating and drying the alcohol solution, and obtaining the extractive of Gynura divaricata; 2) decocting Radix Puerariae with water for 1.5 to 2 hours, wherein the weight of water is 8 to 12 times the weight of Radix Puerariae, collecting the extracting solution; then adding water again into the residue thereof, wherein the weight of water is 5 to 8 times the weight of the residue, deeocting them for 1 to 1.5 hours, collecting the extracting solution again; mixing both extracting solutions, filtrating and concentrating it, then adding alcohol thercto and making the content of alcohol in 70% to 90%, filtrating, concentrating and drying the alcohol solution, and obtaining the extractive of Radix Puerariae; 3) decoeting Radix Paeoniae Alba with water for 1.5 to 2 hours, wherein the weight of water is 8 to 12 times the weight of Radix Paeoniac Mba, collecting the extracting solution; then adding water again into the residue thereof, wherein the weight of water is to 8 times the weight of the residue, decocting them for Ito 1.5 hours, collecting the extracting solution again; mixing both extracting solutions, filtrating and concentrating it, then adding alcohol thereto and making the content of alcohol in 70% to 90%, filtrating, concentrating and drying the alcohol solution, and obtaining the extractive of Radix Paeoniae Alba; and 4) mixing lEe extractives of Gynura divaricata, Radix Puerariae and Radix Paeoniae Mba evenly all together to obtain a total extractive, then adding the pharmaceutical adjuvants thereto, and producing various dosage forms by using proper technology.

After adding pharmaceutical ad] uvants into the total extractive, mixing them evenly, pelleting and drying them, then pills can bc produced by tableling them, or capsules can be produced by packing [hem into capsules, or granules can be produced by packing them.

Oral liquid also can be prepared by the following steps: I) decocting Gynura divaricata with water for 1.5 to 2 hours, wherein the weight of water is 8 to 12 times the weight of Gynura divaricata, collecting the extracting solution; then adding water again into the residue thereof wherein the weight of water is 5 to 8 times the weight of the residue, decocting them for 1 tol.5 hours, collecting the extracting solution again; mixing both extracting solutions, filtrating and concentrating it, then adding alcohol thereto and making thc content of alcohol in 70% to 90%, fIltrating and concentrating the alcohol solution, and obtaining the extractive solution of Gynura divaricata; 2) decocting Radix Puerariae with water for 1.5 to 2 hours, wherein the weight of waler is 8 to 12 times the weight of Radix Puerariae, collecting the extracting solution; then adding water again into the residue thereof, wherein the weight of water is 5 to 8 times thc weight of the residue, decocting them for I to 1.5 hours, collecting the extracting solution again; mixing both extracting solutions, filtrating and concentrating it, then adding alcohol thereto and making the content of alcohol in 70% to 90%, filtrating and concentrating the alcohol solution, and obtaining the extractive solution of Radix Puerariae; 3) decocting Radix Paeoniae Alba with water for [.5 to 2 hours, wherein the weight of water is 8 to 12 times the weight of Radix Paeoniae Atha. collecting the extracting solution; then adding water again into the residue thereof, wherein the weight of water is S to 8 times the weight of the residue, deeoeting them for 1 to 1.5 hours, collecting the extracting solution again; mixing both extracting solutions, filtrating and concentrating it, then adding alcohol thereto and making the content of alcohol in 70% to 90%, filtrating and concentrating the alcohol solution, and obtaining the extractive solution of Radix Paeoniae Alba; and 4) mixing the extractive solutions of (iynura divaricata, Radix Puerariae and Radix Paeoniae Alba evenly all together, and adding corrective thereto to obtain an oral liquid.

The preparation of thc present invention may be taken by oral administration clinically, and the dosage varies according to different dosage forms. Wherein 12 to 20 grams of granules should be taken one day, 10 to 25 pills should be taken one day, 10 to 25 capsules should be taken one day, and 30 to 50 milliliters of oral liquid should be taken one day.

The beneficial effect of the present invention is that effective prescription for lowering lipid and protecting liver can be prepared according to theory of traditional Chinese medicine, whereby modern dosage forms arc prepared by means of modern pharmaceutics, contributing to prominent effect and easy administration of preparation of the present invention. The present invention is capable of lowering lipid and protecting liver, and can be used to prevent and treat hyperlipideniia, obesily, vira.l hepatitis, chemical liver injury, alcoholic liver injury and the like.

DETAILED DESCRIPTION OF TIlE INVENTION

The present invention relates to a Chinese medicine composition for lowering lipid and protecting liver, being a compound preparation prepared by Chinese medicine components and pharmaceutical adjuvants, wherein the Chinese medicine components comprise the formula in parts by weight: 60 to 120 parts of Gynura divaricata, 50 to! 10 parts of Radix Puerariae and 20 to 60 parts of Radix Paeoniae Alba.

Preferably, Gynura divaricata is 80 to 100 parts by weight, Radix Puerariae is 70 to 90 parts by weight and Radix Paeoniae Alba is 30 to 50 parts by weight.

Wherein, Gynura divaricata is the whole herb of Gynw-a divariccaa (L.) DC'. [(n mm 115 IX,'. C. pseudo--china (IL) DC. I of feverfew, Radix Puerariae is the dred root of Pueraria lobaici ([Vilici. )Ohwi of leguminosae, Radix t'aeoniae Mba is the dried root of Paeonia lacqfiora Pail, of ranunculaeeae.

The pharmaccutical adjuvant is one of, or a mixture of two or more of dextrin, saccharose, lactose, starch, sodium carboxymethyl starch, polyvinylpyrrolidone, and magnesium stearate.

The compound preparation is in the forms of capsules, pills, granules, or oral liquid.

The preparation method of the above Chinese medicine composition comprises the following steps: 1) decocting Gynura divaricala with water for 1.5 to 2 hours, wherein the weight of water is 8 to 12 times the weight of Gynura divaricata, collecting the extracting solution; then adding water again into the residue thereof wherein the weight of water is S to 8 times the weight of the residue, decocting them for 1 to 1.5 hours, collecting the extracting solution again; mixing both extracting solutions, filtrating and concentrating it, thcn adding alcohol thereto and making the content of alcohol in 70% to 90%, filtrating, concentrating and drying the alcohol solution, and obtaining the extractive of Gynura divaricata; 2) decocting Radix Fuerariae with waler for 1.5 to 2 hours, wherein the weight of water is 8 to 12 times the weight of Radix Puerariae. collecting the extracting solution; then adding water again into the residue thereof, wherein the weight of water is 5 to 8 times the weight of the residue, decocting them for I to 1.5 hours, collecting the extracting solution again; mixing both extracting solutions, filtrate and concentrate it, then adding alcohol thereto and making the content of alcohol in 70% to 90%, filtrating, concentrating and drying the alcohol solution, and obtaining the extractive of Radix Puerariae; 3) decocting Radix Paeoniae Alba with water for 1.5 to 2 hours, wherein thc weight of water is 8 to 12 times the weight of Radix Paeoniae Alba, collecting the extracting solution; then adding water again into the residue thereof, wherein the weight of water is to 8 times the weight of the residue, decocting them for ito 1.5 hours, collecting the extracting solution again; mixing both extracting solutions, filtrating and concentrating it, then adding alcohol thereto and making the content of alcohol in 70% to 90%, filtrating, concentrating and drying the alcohol solution, and obtaining the extractive of Radix Paeoniae Alba; and 4) mixing the extractives of Gynura divaricata, Radix Puerariae and Radix PaeoniaeAlba evenly all together to obtain a total extractive, then adding pharmaceutical adjuvants thereto, and producing various dosage forms by proper processes. For exmnple, after adding phannaceutical adjuvants into the total extractive, mixing them evenly, pelleting and drying them, then pills can be produced by tableting them, or capsules can be produced by packing them into capsules, or granules can be produced by packing them.

The preparation method is ftLrther explained by the following embodiments, which should not he deemed to limit the seopc of the present invention.

Embodimcnt I 90g Gynura divarieata is decocted with water for 2 hours, wherein the weight of water is 1 0 times the weight of Gynura divaricata, then the extracting solution is collected. Water is added again into the residue thereof to be decocted for 1.5 hours, wherein the weight of water is 6 times the weight of the residue, then the extracting solution is also collected. Both extracting solutions are mixed, and the mixed solutions are filtrated and concentrated to a relative density of 1.10 to 1.12, then alcohol is added thereto until content of alcohol reaches 85%. The alcohol solution is filtrated, concentrated and dried thereafter, whereby the extractive of (lynura divaricata can be obtained, 80g Radix Puerariae is decocted with water for 2 hours, wherein the weight of water is 10 times the weight of Radix PLlerariae, then the extracting solution is collected. Water is added again into the residue thereof to be decoeted for 1.5 hours, wherein the weight of water is 6 times the weight of the residue, then the extracting solution is also collected. Both extracLmg solutions are mixed, and the mixed solution are filtrated and concentrated to a relative density of 1.20 to 1.30, then alcohol is added thereto until content of alcohol reaches 90%.

The alcohol solution is filtrated, concentrated and dried thereafter, whereby the extractive of Radix Puerariae can be obtained.

40g Radix Paeoniac Alba is deeocted with water for 2 hours, wherein the weighi of water is 10 times the weight of Radix Paeoniae Alba, then the extracting solution is collected. Water is added again frito the residue thereof to be decocted for 1.5 hours, wherein the weight of water is 6 times the weight of the residue, then the extracting solution is also collected. Both extracting solutions are mixed, and the mixed solution are filtrated and concentrated to a relative density of 1.15 to 1.25, then alcohol is added thereto until content of alcohol reaches 80%. The alcohol solution is filtrated, concentrated and dried thereafter, whereby the extractive of Radix Paeoniae Mba can be obtained.

The extractives of Gynura divaricata, Radix Puerariae and Radix Paeoniae Alba are mixed evenly all together, appropriate amount of magnesium stearate is added thereto to form a mixture, which will be encapsulated eventually.

Example 2

l20g Gynura divaricata is decocted with water for 1 hour, wherein the weight of water is 8 times the weight of Gynura divaricata, then the extracting solution is collected. Water is added again into the residue thereof to be decocted for 1.5 how's, wherein the weight of water is 8 times the weight of the residue, then the extracting solution is also collected. Both extracting solutions are mixed, and the mixed solutions are filtrated and concentrated to a relative density of 1.10 to 1.12, then alcohol is added thereto until content of alcohol reaches 90%. The alcohol solution is filtrated, concentrated and dried thereafter, whereby the extractive of Gynura divaricata can he obtained.

600g Radix Puerariae is decocted with water for 1 horn; wherein the weight of water is 12 Limes the weight of Radix Puerariae, then the extracting solution is collected. Water is added again into the residue thereof to be dccocted for 1.5 hours, wherein (he weight of water is S times the weight of the residue. then the extracting solution is also collected. Both extracting solutions are mixed, and thc mixcd solution are filtrated and concentrated to a relative density of 1.10 to 1.20, then alcohol is added thereto until content of alcohol reaches 90%.

20g Radix Paeoniae Alba is decocted with water for 1.5 hours, wherein the weight of water is 12 times the weight of Radix Paeoniae Alba, then the extracting solution is collected.

Water is added again into the residue thereof to be decocted for 1.5 hours, wherein the weight of water is 8 times the weight of the residue, then the extracting solution is also collected. Both extracting solutions are mixed, and the mixed solution are filtrated and concentrated to a relative density of 1.10 to 1.20, then alcohol is added thereto until content of alcohol reaches 70%. The alcohol solution is filtrated, concentrated and dried thereafter, whereby the extractive of Radix Paeoniae Alba can he obtained.

The extraetives of Gynura divaricata, Radix Puerariac and Radix Paeoniae Alba are mixed evenly all together, appropriate amount of magnesium stearate is added thereto to form a mixture, which will be encapsulated eventually.

Example 3

60g Gynura divaricata is decoded with water for 2 hours, wherein the weight of water is 12 times the weight of Gynura divaricata, then the extracting solution is coflected. Water is added again into the residue thereof to be decocted for I.5 hours, wherein the weight of water is 10 times the weight of the residue, then the extracting solution is also collected.

Both extracting solutions are mixed, and the mixed solutions are filtrated and concentrated to a relative density of 1.10 to 1.12, then alcohol is added thereto until content of alcohol reaches 80%. The alcohol solution is filtraled, concentrated and dried thereafter, whereby the extra.ctive of Gynura divaricata can he obtained.

SOg Radix Puerariae is decoeted with watcr for 2 hours, wherein the weight of water is 12 times the weight of Radix Puerariae. then the extracting solution is collected. Water is added again into the residue thereof to he decocted for 1.5 hours, wherein the weight of water is 10 times the weight of thc rcsidue, then the extracting solution is also collected. Both cxtracting solutions are mixed, and the mixed solution are filtrated and concentrated to a relative density of 1.10 to 1.15, then alcohol is added thereto until content of alcohol reaches 80%.

60g Radix Paeoniae Alba is decocted with water for 2 hours, wherein the weight of water is 8 times the wcight of Radix I'aeoniae Alba, then the extracting solution is collected. Water is added again into the residue thereof to be decocted for 1.5 hours, wherein the weight of water is 6 times the weight of the residue, then the extracting solution is also collected. Both extracting solutions arc mixcd. and the mixed solution are filtrated and concentrated to a relative density of 1.10 to 1.18, then alcohol is added thereto until content of alcohol reaches 7O%. The alcohol solution is filtrated, concentrated and dried thereafter, whercby the extractive of Radix Paeoniae Alba can be obtained.

The extractives of Gynura divaricata, Radix Puerariae and Radix Paeoniae Alba are mixed evenly all together. appropriate amount of magnesium stearate is added thereto to form a mixture, which will be encapsulated eventually.

Example 4

90g (lynura divaricata is decocted with water for 2 hours, wherein tile weight of water is 1 0 times the weight of Gynura divaricata, then the extracting solution is collected. Water is added again into thc rcsiduc thereof to be decocted for 1.5 hours, wherein the weight of water is 6 times the weight of the residue, then the extracting solution is also collected. Both extracting solutions are mixed, and the mixed solutions are filtrated and concentrated to a relative density of 1.10 to 1.12, then alcohol is added thereto until content of alcohol reaches 85%. The alcohol solution is filtrated, concentrated and dried thereafter, whereby the extractive of Gynura divaricata can he obtained.

IS 80g Radix Puerariae is decocted with water for 2 hours, wherein the weight of water is 10 times the weight of Radix Puerariae, then the extracting solution is collected. Water is added again into the residue tlicreof to be decoded for 1.5 hours, wherein the weight of water is 6 times the weight of the residue, then the extracting solution is also collected. Both extracting solutions are mixed, and the mixed solution are filtrated and concentrated to a relative density of 1.20 to 1.30, then alcohol is added thereto until content of alcohol reaches 90%.

40g Radix Paeoniae Alba is decocted with water for 2 hours, wherein the weight of water is 10 times the weight of Radix Paeoniae Mba. then the extracting solution is collected. Water is added again into the residue thereof to be decocted for 1.5 hours, wherein the weight of water is 6 times the weight of the residue, then the extracting solution is also collected. Both extracting solutions are mixed, and the mixed solution are filtrated and concentrated to a relative density of 1.15 to 1.25, then alcohol is added thereto until content of alcohol reaches 80%. The alcohol solution is filtrated, concentrated and dried thereafter, whereby the extractive of Radix Paeoniae Alba can be obtained.

The extractives of Gynura divaricata, Radix Puerariae and Radix Paeoniae Alba are mixed evenly all together, appropriate amount of magnesium stearate and dextrin are added thereto to form a mixture, which will be produced to tablets by dtying and tableting it eventually.

Example 5

120g Clynura divaricata is decocted with water for 1 hour, wherein the weight of water is 8 times the weight of Gynura divarieata, then the extracting solution is collected. Water is added again into the residue thereof to be decocted for 1.5 hours, wherein the weight of water is 8 times the weight of the residue, then the extracting solution is also collected. Both extracting solutions arc mixed, and the mixed solutions are filtrated and concentrated to a relative density of 1.10 to 1.12, then alcohol is added thereto until content of alcohol reaches 90%. The alcohol solution is filtrated, concentrated and dried thereafter, whereby the extractive of Gynura divaricata can he obtained.

600g Radix Pucrariae is decoded with water for 1 hour, wherein the weight of water is 12 times tFe weight of Radix Puerariae, then the extracting solution is collected. Water is added again into the residue thereof to be decocted for 1.5 hours, wherein the weight of water is 8 times the weight of the residue, then the extracting solution is also collected. Both extracting solutions are mixed, and the mixed solution are filtrated and concentrated to a relative density of 1.10 to 1.20, then alcohol is added thereto until content of alcohol reaches 80%.

20g Radix Paeoniae Mba is decocted with water for 1.5 hours, wherein the weight of water is 12 times the weight of Radix Paeoniae Aiha, then the extracting solution is collected.

Water is added again into (Fe residue thereof to be decocted for 1.5 hours, wherein the weight of water is 8 times the weight of the residue, then the extracting solution is also collected. Both extracting solutions are mixed, and the mixed solution are filtrated and concentrated to a relative density of 1 0 to I.20, then alcohol is aMed thereto until content of alcohol reaches 70%. The alcohol solution is filtrated, concentrated and dried thereafter, whereby the extractive of Radix Paeoniae Alba can be obtained.

The extractives of Gynura divaricata, Radix Puerariae and Radix Paeoniae Mba are mixed evenly all together, appropriate amount of magnesium stearatc and dextrin are added thereto to form a mixture, which will be produced to tablets by tableting it eventually.

Example 6

óOg Gynura divarleata is decocted with water for 2 hours, wherein the weight of water is 12 timcs the weight of Gynura divaricata, then the extracting solution is collected. Water is added again into the residue thereof to be decoeted for 1.5 hours, wherein the weight of water is 10 times the weight of the residue, then the cxtracting solution is also collected.

Both extracting solutions are mixed, and the mixed solutions are filtrated and concentrated to a relative density of 1.10 to 112, then alcohol is added thereto until content of alcohol reaches 80%. The alcohol solution is filtrated, concentrated and dried thercafler. whereby the extractive of Gynura divaricata can be obtained.

SOg Radix Puerariae is decoeted with water for 2 hours, wherein the weight of water is 12 times the weight of Radix Puerariae. then the extracting solution is collected. Water is added again into the residue thereof to be decocted for 1.5 hours, wherein the weight of water is 10 times the weight of the residue, then the extracting solution is also collected. Both extracting solutions are mixed, and the mixed solution are filtrated and concentrated to a relative density of 1.10 to 1.15, then alcohol is added thereto until content of alcohol reaches 80%.

The alcohol solution is filtrated, concentrated and dried thereafler, whereby the extractive of Radix Puerariae can be obtained.

60g Radix Paeoniae Mba is decoeted with water for 2 hours, wherein the weight of water is 8 times the weight of Radix Paeoniae Alba, then the extracting solution is collected. Water is added again into the residue thereof to be decocted for 1.5 hours, wherein the weight of water is 6 times the weight of the residue, then the extracting solution is also collected. Both extracting solutions are mixed, and the mixed solution are filtrated and concentrated to a relative density of 1.1 0 to 1.18, then alcohol is added thereto until content of alcohol reaches 70%. The alcohol solution is filtrated, concentrated and dried thereafter, whereby the extractive of Radix Paeoniae Alba. can be obtained.

The extractives of Gynura divaricata, Radix Puerariae and Radix Paeoniae Alba are mixed evenly all togetliei; appropriate amount of magnesium stearate and dextrin are added thereto to form a mixture, which will be produced to tablets by tableting it eventually.

Example 7

90g Gynura divaricata is decocted with water for 2 hours, wherein the weight of water is 10 times the weight of Gynura divaricata, then the extracting solution is collected. Water is added again into the residue thereof to be decocted for 1.5 hours, wherein the weight of water is 6 times the weight of the residue, then the extracting solution is also collected. Both extracting solutions are mixed, and the mixed solutions arc filtrated and concentrated to a relative density of 1.10 to 1.12, then alcohol is added thereto anti I content of alcohol reaches 85%. The alcohol solution is filtrated, concentrated and dried thereafter, whereby the extractive of Gynura divaricata can be obtained.

80g Radix Puerariae is decoeted with water for 2 hours, wherein the weight of water is 10 times the weight of Radix Puerariae, then the extracting solution is collected. Water is added again into the residue thereof to be decoeted for 1.5 hours, wherein the weight of water is 6 times the weight of the residue, then the extracting solution is also collected. Both exnacting solutions are mixed, and the mixed solution are filtrated and concentrated to a relative density of 1.20 to 1.30, then alcohol is added thereto until content of alcohol reaches 90%.

The alcohol solution is filtrated, concentraled and dried thereafter, whereby the extractive of Radix Puerariae can be obtained.

40g Radix Paeoniae Atha is decoeted with water for 2 hours, wherein the weight of water is 10 times the weight of Radix Paeoniac Alba. then the extracting solution is collected. Water is added again into the residue thereof to be decocted for 1.5 hours, wherein the weight of water is 6 times the weight of the residue, then the extracting solution is also collected. Both extracting solutions are mixed, and the mixed solution are filtrated and concentrated to a relative density of US to 1.25, then alcohol is added thereto until content of alcohol reaches 80%. The alcohol solution is filtrated, concentrated and dried thereafter, whereby the cxtraetivc of Radix Paeoniae Alba can be obtained.

The extractives of Gynura divaricata, Radix Puerariae and Radix Paeoniae Alba are mixed evenly all togethei; appropriate amount of magnesium stearate and dextrin are added thereto to form a mixture, which will be produced to granules by pelleting it eventually.

Example 8

120g Gynura divaricata is decocted with water for 1 hour, wherein the weight of water is 8 times the weight of Gynura divaricata, then the extracting solution is collected. Water is added again into the residue thereof to be decoeted for 1.5 hours, wherein the weight of water is 8 limes the weight of the residue, then the extracting solution is also collected. Both extracting solutions are mixed, and the mixed solutions are filtrated and concentrated to a relative density of 1.10 to 1.12, then alcohol is added thereto until content of alcohol reaches 90%. The alcohol solution is filtrated, concentrated and dried thereafter, whereby the extractive of Gynura divaricata can be obtained.

600g Radix Puerariae is decoeted with water for 1 hour, wherein the weight of water is 12 times the weight of Radix Puerariae, then the extracting solution is collected. Water is added again into the residue thereof to be deeocted for 1.5 hours, wherein the weight of water is 8 times the weight of the residue, then the extracting solution is also collected. Both extracting solutions are mixed, and the mixed solution are filtrated and concentrated to a relative density of 1.10 to 1.20, then alcohol is added thereto until content of alcohol reaches 80%.

Water is added again into the residue thereof to be decocted for 1.5 hours, wherein the weight of water is 8 times the weight of the residue. then the extracting solution is also collected. Roth extracting solutions are mixed, and the mixed solution are filtrated and concentrated to a relative density of 1.10 to 1.20, then alcohol is added thereto until content of alcohol reaches 70%. The alcohol solution is filtrated, concentrated and dried thereafter, whereby the extractive of Radix Paeoniae Affia can be obtained.

The extractive of Gynura divaricata, Radix Puerariae and Radix Paeoniae Alba are mixed evenly all together, appropriate amount of maiesium stearate and dextrin are added thereto to fom a mixture, which will be produced to granules by pelleting it eventually.

Example 9

60g Gynura divarieata is decoeted with water for 2 hours, wherein the weight of water is 12 times the weight of Gynura divaricata, then the extracting solution is collected. Water is added again into the residue thereof to be deeocted for 1.5 hours, wherein the weight of water is 10 times the weight of the residue, then the extracting solution is also collected.

Both extracting solutions are mixed, and the mixed solutions are filtrated and concentrated to a relative density of 1.10 to L12, then alcohol is added thereto until content of alcohol reaches 80%. The alcohol solution is filtrated, concentrated and dried thereafter, whereby the extractive of Gynura divaricata can be obtained.

50g Radix Puerariae is decocted with water for 2 hours, wherein the weight of water is 12 times the weight of Radix Puerariae, then the extracting solution is collected. Water is added again into the residue thereof to be decocLed for 1.5 hours, wherein the weight of water is 10 times the weight of the residue, then the extracting solution is also collected. Both extracting solutions are mixed, and the mixed solution are filtrated and concentrated to a relative density of 1.1 0 to 1.15, then alcohol is added thereto until content of alcohol reaches 80%.

60g Radix Paeoniae Alba is decoded with water for 2 hours, wherein the weight of water is 8 times the weight of Radix Paeon inc Alba, then the extracting solution is collected. Water is added again into the residue thereof to be decocted for 1.5 hours, wherein the weight of water is 6 times the weight of the residLLe, then the extracting solution is also collected. Both extracting solutions are mixed, and the mixed solution are filtrated and concentrated to a relative density of 1.10 to 1.18, then alcohol is added thereto until content of alcohol reaches 70%. The alcohol solution is filtrated, concentrated and dried thereafter, whereby the extractive of Radix Paeoniae Alba can be obtained.

The extractives of Gynura divaricata, Radix Pucrariac and Radix Paeoniae Mba are mixed evenly all together, appropriate amount of magnesium stearate and dextrin are added thereto to form a mixture, which will be produced to granules by pelleting it eventually.

Example 10

60g Gynura divaricata is decocted with water for 2 hours, wherein the weight of water is 12 times the weight of Gynura divaricata, then the extracting solution is collected. Water is added again into the residue thereof to be decocted for 1.5 hours, wherein the weight of water is 10 times the weight of the residue, then the extracting solution is also collected.

Both extracting solutions are mixed. and the mixed solutions are filtrated and concentrated to a relative density of 1.10 to 1.12, then alcohol is added thereto until content of alcohol reaches 80%. The alcohol solution is filtrated and concentrated thereafter, whereby the extractive of Gynura divaricata can be obtained.

50g Radix Puerariae is decocted with water for 2 hours, wherein the weight of water is 12 times the weight of Radix Puerariae, then the extracting solution is collected. Water is added again into the residue thereof to be decocted for 1.5 hours, wherein the weight of water is 10 times the weight of die residue, then the extracting solution is also collected. Both extracting solutions are mixed, and the mixed solution are filtrated and concentrated to a relative density of 1.10 to 1.15, then alcohol is added thereto until content of alcohol reaches 80%.

The alcohol solution is filtrated and concentrated thereafter, whereby the extractive of Radix Puerariae can be obtained.

60g Radix Paeoniae Alba is decoeted with water for 2 hours, wherein the weight of water is 8 times the weight of Radix Paeoniae Alba, then the extracting solution is collected. Water is added again into the residue thereof to be decocted for 1.5 hours, wherem the weight of water is 6 times the weight of the residue, then the extracting solution is also collected. Both extracting solutions are mixed, and the mixed solution are filtrated and concentrated to a relative density of 1.10 to 1.18, then alcohol is added thereto until content of alcohol reaches 70%. The alcohol solution is filtrated and concentrated thereafier, whereby the extractive of Radix Paeoniae Alba can be obtained.

The exiraetives of Gynura divaricata, Radix Pucrariac and Radix Paeoniae Alba are mixed evenly all togethei; appropriate amount of stevioside is added thereto to form a mixture, which will be an oral liquid eventually.

Example 11

Research on the pharmaeodynaniics of lowering lipid 1. Materiak and Method Tested medicines: content of capsules prepared according to Example 1.

Simvastatin tablets, provided by Hangzhou Merck Sharp & Dohme Pharmaceutical Co., Ltd.; Batch number: 110689.

Animals: 70 SD male rats, SPF grade, each having weight of 170 to 200 g. Quality certificate number for laboratory animal: 2006A051; Certificate number of approved environment condition for SPF laboratory animal: 2006B023; Number of production licence of SPE laboratory animal: S'CXK GD-20 11-001 5; animals are supplied by Animal Laboratory Center of Southern Medical University.

High-fat feed: 12% lard oil (homemade), 2% cholesterol (Aladdin Industrial Corporation; Batch number: 1216021), 0.2% propylthiouracil (Guangdong Southern China Pharmaceutical Co., Ltd.; Batch number: 120601), 0.5% 3# cholate (Guangdong Huankai Microbial Sci. & Tech Co., Ltd.: Batch number: 3201077), 85.3% ordinary mixed feed meal (Animal Laboratory Center of Southern Mcdical University); Mix above materials evenly manually, and stir them with machine, press to make granules of round strips, place them in the freezer and chill for later use. The high-fat feed is prepared by Animal Laboratory Center of Southern Medical University.

Primary chemical reagents and detecting instruments: formaldehyde solution with grade of analytical reagent and batch number: 20111007, supplied by Guangdong Guanghua Sci-Tech Co., Ltd.; OLYMPUS BH-2 fluorescence microscope, supplied by OLYMPUS Corporation in Japan; BECKMANTM3O refrigerated centrifuge, supplied by BACKMAN Corporation in USA; LETCA RM2I 35 microtome, supplied byLEICA Corporation in Germany; ECHO LCD biochemical analyzer supplied by E1CAM Corporation in Ttaly.

Experimental methods and dosages: animals are divided into normal group, model group, sirnvastatin group (10mg/kg), high dose group (l.l2gIkg), medium dose group (0.56g/kg), low dose group (0.28g/kg), and very low dose group (0.14g/kg. There arc 10 animals in each group. Except the normal group, around 20g high-fat feed, instead of normal feed, is fed to each animal of other groups per day. Tap water is poured into stomachs of rats in the normal group and model group, and medicines are poured into stomachs of rats in other medicated groups once every afternoon with a dosage of lini/lOOg per rat. The experiment lasts for 21 days since started. After the last dosing in the evening before the experiment finishes, any feed is forbidden, but water could be supplied normally. In the next forenoon, blood collection of abdomen aorta is conducted after anesthesia, and then sent to Medical Testing Center of Southern hospital of Southern Medical Universi to test TG(triglyceride), CHO(total Cholesterol), HDLigh density lipoprotein) and LDL(low density lipoprotein).

At site which is 1.5 cm away from edge of left lobe of liver, transect a liver tissue, lix it in neutral formalin liquid, and make lIE stain after conventional dehydration. The experimental data is illustrated by 1±SD and rank/frequency table, and processed by spss 8.0 statistical software, i.e. One-Way ANOVA LST, or method of DunnettT3 and Nonparametric Test 2 Independent Samples Tests.

2. lest result 1) weight changes of rats The weight changes of rats are illustrated in Table I. Table I. Weight Changes of Rats in Different groups (x±SD, N6) Group Animal Weight in Weight in Weight in Weight in Percent of (number) Day 1(g) Day 8(g) Day 15 (g) Day 22(g) increased weight (%) Normal group 10 211±30 262±34 292±31* 314±31** 50±14** Model group 10 210±16 250±21 266120 247±21 18±8 Simyastatin 10 210+26 249+27 271+26 250+22 20±8 group Highdose 10 f 210+21 255+29 2744:28 250±28 19±8 group Medium dose 10 210+22 250±25 266±18 252±18 21+7 group Low dose 10 210+21 253±24 271±23 249±21 19±6 group Verylowdose 10 211+22 252±26 270±28 254±26 21±12 group Remark: a. * P<0.05 as compared with the Model group; ** P<0.0l as compared with the Model group.

b. percent of increased weight =(weight in Day 22-weight in Day 1)/weight in Day 1>< 100.

J

It could be seen from Table 1 that in the model group, the weight of rat in Day 15 and Day 22, and percent of increased weight thereof are obviously lower than that in the normal group (P<0.05, P<OM1, P<0.0l); there are no obvious differences between other groups and the model group.

2) Feed Consumption Feed consumption amount is illustrated in Table 2.

Table 2. Feed Consumption Amount of Rats in Different Groups Group Animal Total feed Average feed Feed consumption (number) consumption consumption amount for every increasing amount (g) per rat (g) ig weight (g) nonnal group 10 3967 397 3.8 model group 10 3596 360 9.5 simvastatin 10 3591 359 9.0 group high dose group 10 3675 368 9.2 mediumdose 10 3556 356 8.5 group low dose group 10 3599 360 9.2 very low dose 10 3640 364 8.7 group IS It could be seen from Table 2 that the average feed consumption amounts per rat are very close. In normal group, the feed consumption for every ig increased weight is minimum.

[here arc no obvious differences between other groups and the model group.

3) lest results of blood fat The test results of blood fat of rats are illustrated in Fable 3.

Table 3. lest Results of Blood Fat of Rats in Different Groups (±SD) Group Animal TO 010 HDL LDL Ratio of (number) (minol!L) (mmol/L) (mniol/L) (nirnol/L) I-Ill' _________ _______ _______ ______ ________ _______ (3⁄4) Normal group 10 0.20±0.06 136±025 067±014* 047±008** 49+3** Model group 10 0.29±0.08 736±262 104±024 566±205 15±4 Simvastatin 10 0.18±0.04 541±177 084±023 423±145 15±4 group High dose 10 0.16±0.04 560±209 087±028 431±159 16±4 group Medium dose 10 0.18±0.03 579±082 094±017 443±067 16±3 group Low dose 10 0.15±0.02 594±302 094±037 462±242 17±4 group Very low dose 10 0.19±0.04 597±169 088±026 464±135 15±2 group S Remark: a. * P<0.05 as compared with the Model group; ** P<0.01 as compared with the Model group.

b. Ratio of HIT= FTDL cholesterol! CHO >C 100%.

c. CHO HDL (cholesterol) + LDL 1-VLDL It could be seen from Table 3, compared with the nonnal group, the contents of total serum cholesterol and LDL cholesterol in the model group increase obviously (P<0.0l), and the ratio of HJT decreases obviously (P<0.0l); compared with the model group, contents of serum TO in the simvastatin group, high dose group, medium dose group, low dose group decrease obviously (P<0.05, P<0.05, P<0.05, P<0.0i); compared with the model group, contents of total serum cholesterol in each dosing group decrease to some extent, but without obvious difference.

4) Histopathologic examination of liver The results of histopathologic examination of liver are illustrated in Table 4.

Table 4: Results of Ilistopathologic Examination of the Rat Liver in different groups Group Normal liver Low-grade Medium -grade Severe-grade P tissue (-) fatty-liver (+) fatty-liver (-f-i-) fatty-liver value (number) (number) (number) (-f-++) (number) Normal group 0 5 5 0 0.001 Model group 0 0 4 6 Simvastatin 0 0 5 5 0.66 1 group Highdose 0 0 7 3 0.189 group Medium dose 0 1 6 3 0.147 group Lowdose 0 0 8 2 0.075 group Very low dose 0 0 7 3 0.189 group Remark: changes of fatty-liver: symbol "+" indicates that the structure of hcpatic lobule still exists, and the structure of liver tissue is generally normal, parts of hepatic cords arrange at random, hepatic sinusoid is getting narrow, and liver cells around portal area are swelling, with low-grade vacuolization and ballooning degeneration; symbol "++" indicates blurry sthicture of hepatic lobule, disappearance of parts of hepatic cords, disordered arrangement of liver cell, swelling, vacuolization and ballooning degeneration of most liver cells, .spo necrosis and focal necrosis of liver cells in parts of animals, and little inflammatory cell infiltration iii poil area; symbol "+-i-±" indicates the disappearance of structural outline of hepatic lobule, irregular shape of liver cells, ballooning degeneration and swelling of the very most of liver cells, vacuolization of parts of liver cells, spotty necrosis and piecemeal necrosis of liver cells in parts of animals, and inflammatory cell infiltration in portal area.

It could be seen from I'ahle 4, compared with the normal group, fatty degeneration of rat liver in model group is severe (P<0.0l); compared with the model group, the fatty degeneration of rat liver is relieved to some extent in each dosing group, especially in the low dose group, which is close to the significant difference (P-O.075), and it is corresponding to the observation on the liver generally.

3. Experimental Conclusion

Compared the model group with the normal group, in the model group, the weight decreases obviously, the contents of serum total cholesterol and LDL cholesterol increase obviously, the ratio of H/T decreases obviously, and obvious swelling of liver cells (fatty degeneration) could he seen.

Compared with the model group, the contents of serum TG of rat in the high dose group, medium dose group and low dose group decrease obviously. The contents of serum total cholesterol of rats decrease to some extent in each dosing group, but without obvious difference.

Compared with the model group, the fatty degeneration of rat liver is relieved to some extent in each dosing group, especially in the low dose group.

Example 12

Research on the phannacodynamics of protecting liver L Materials and Method 1) Test Medicines: content of capsules prepared according to Example I. Bifendate pills, supplied by Guangzhou Xingqun Pharmaceutical Co., Ltd., with batch number: SF40021.

2) Animals Animals: 110 NIH male mice with SPF grade, each having weight of 18 to 22g. Quality certificate number of laboratory animal: 2006A05 I; Certificate number of approved environment condition of laboratory animal with SPF grade: 2006B023; Number of production licence of SPF laboratory animal: SCXK GD-20 11-0015; animals are supplied by Animal Laboratory Center of Southern Medical University.

3) Primary chemical reagents and detecting instruments: reagent kits of alanine arninotransferase (ALT), supplied by BioSino Bio-technology and Science Inc., with batch number: 121881; reagent kits of aspartate aminortransferase (AST), supplied by BioSino Bio-technology and Science Inc., with batch number: 120921; carbon tetrachloride solution of analytically pure grade, supplied by Tianjin Funing Fine Chemical Engineering Co., Ltd.. with batch number: 111010; formaldehyde solution of analytically pure grade, supplied by Guangdong Guanghua Sci-Tech Inc., with batch number: 20111007; absolute ethyl alcohol of analytically pure grade, supplied by Tianjin.iinfeng Chemical Engineering Co., Ltd., with batch number: 20110416; xylcne of analytically pure grade, supplied by Guangdong Guanghua Chemical Plant Co., Ltd., with batch number: 20101224; Hematoxylin, which is a biological chromosin and subpackaged by Beijing Dingguo Biotechnical Co., Ltd., with patch number: 060408; Eosin Y, which is a biological chromosin and supplied by Chinese East China Normal University Chemistry Plant, with patch number: 20050912; ECHO LCD biochemical analyzer supplied by EChO Corporation in Italy; OLYMPUS AU5421 automatic biochemical analyzer, supplied by OLYMPUS Corporation in Japan; OLYMPUS BH-2 fluorescence microscope, supplied by OlYMPUS Corporation in Japan; BECKMANTM3O refrigerated centrifuge, slLpplied by BACKMAN Corporation in USA; LEICA RM21 35 microtome, supplied by LEICA Corporation iii Germany; SARTORIUS electronic balance, supplied by SARTORfUS Corporation in Germany.

4) Experimental method and doses: animals are divided into normal group, model group, bifendate group (0.2g/kg), high dose group (l.56g/kg), medium dose group (0.78g/kg), and low dose group (0.39g/kg. There are 12 animals in each group. Medicines are poured into stomach once everyday for 7 days. For normal group and model group, the same volume tap water is poured. The medicine dose is 0.25 mI/lOg per mouse, with voluntary feeding.

Conduct intraperitoneal inject of O.2m1 carbon tetrachioride (prepared by pure peanut oil) with a volume ratio of 0.12 per mouse, one hour after the last hitragastric administration.

Feeding is forbidden, but water could be supplied normally. 16 hours later, pick eyeballs to draw blood, and centriftige the blood to obtain serum for testing glutamic-pyruvic transaminase and glutamic oxalacetic transaminase.

5) In pathological examination, take left lobe of liver, fix it in neutral formalin liquid, make conventional dehydration, embed it in paraffin and cut sections (4 micrometer), make HE stain and observe the pathologic changes of liver tissue through light microscope. liver tissue injury is divided into four grades: Grade 0(-), which means normal structure of liver tissue, non-obvious degeneration, necrosis and inflammatory cell infiltration; Grade I (+), which means normal structure of hepatic lobule, view of turbid and swelling of parts of liver cells, ballooning degeneration or fatty degeneration, and spotty necrosis; Grade II (++), which means indistinct structure of hepatie lobule, obvious focal necrosis accompanying with inflammatory cell infiltration; Grade HI (-!-H-), which means indistinct structure of hepatie lobule, view of obvious piecemeal necrosis accompanying with inflammatory cell infiltration.

6) The experimental data is illustrated by ±SD and rank/frequency table, and processed by spss 8.0 statistical software, i.e. One-Way ANOVA LST, or method of DunnettT3 and Nonparametric Test 2 Independent Samples Tests.

2. Test Results 1) Weight changes of mice Weight changes of micc could be seen from TableS, there are no obvious differences between mice in different groups.

Table 5. Weight Changes of Mice in Different Groups (k±SD, N6) Group Weight in Day I (g) Weight in Day 7(g) Empty body weight in Day 8 (g) Normal group 23.42±1.89 31.94±2.05 27.75J.l.83 Model group 23.51±1.05 3 1.38+1.12 2742±0.96 Sinwastatin 23.81+2.31 30.58±1.89 26.74±1.56 group High dose 23.74+2.53 3 1.89±2.26 27.52±2.22 group Medium dose 23.82±1,87 30.39±1.62 26.47+1.44 group Low dose 23.77±2.00 30.94±2.96 27.10±2.49 group 2) Test results of liver function The test results of serum glutamic pyruvic transaminase (SGPT) and serum glutamic oxalacetic transaminase (SCOT) of mice in differenE groups could be seen from Fable 6.

S Table 6. Test Results of SGPT and SGO'l' of Mice in Different Groups (+SD, N6) Group Dosage OPT GOT ___________ ______ (g/kg) (tilL) _______ (U/L) ______ Normal group 43±6* 1l3±[7** Model group 317±112 303±96 Bifendate group 0.2 47+9** 240±73 Highdosegroup 1.56 165±56 117±35* Medium dose group 0.78 223±114 245±62 Low dose group 0.39 223+140 232+89 Remark: * Pc0.05 as compard with the Model group; P<0.01 as compared with the Model group.

It could be seen from Table 6, compared the model group with the normal group, contents of SGPT and SGOT of the model group increase obviously (P<0.01); Compared with the model group, content of SGPT in Bifendate group decreases obviously (P<0.01), contents of SGPT and SOOT in high dose group decrease obviously (PcO.OS), and in the medium dose group and low dose group, decline of enzyme could be seen partly, but it is not useful for statistical significance due to different variances; There is no obvious difference between other dosing groups and the model group.

3) Effects on pathologic lesion of liver tissue of mouse having acute liver injury As seeu from the results of pathological section, the structure of mouse liver tissue in the normal group is normal, live cells arrange radially around central veins, hepatic cords and hepatic sinusoid arrange regularly, the structurc of hepatic lobule is intact, pathologic changes, such as slight swelling and spoiled necrosis of parts of liver cells, could be seen. Tn the mice of CC 14 model group, most liver cells arranged around central veins are turbid and swelling, the cytoplasm is loose, and ballooning degeneration is occurred partly. Spotted necrosis focal necrosis, or piecemeal necrosis arranged below central veins of hepatic lobule and capsula of liver, accompanying with pathologic changes, such as inflammatory cell infiltration. In the Bifendate group and high dose grolLp, the necrosis of liver ccli is relieved obviously (P<0.0S), proving protection effect on liver injury caused by CC14 intraperitoneal injection in the high dose group. The pathologic results could be seen from Table 7.

Table 7. Effect on Pathologic Lesion of Mouse liver Tissue having Acute Liver Injury in Different Groups Group Degeneration of liver P value Necrosis of liver cell P value cdl -+ +* +++ -± ±4-+1--b-Normal 6 5 1 0 0.000 7 4 1 0 0.000 group Modelgroup 0 1 10 1 0 1 6 5 Bifendate 0 6 4 2 0.149 2 6 2 2 0.011 group Highdose 0 4 6 2 0.446 2 3 6 1 0.021 group Medium dose 0 5 6 1 0.126 1 2 5 4 0.416 group Lowdose 0 3 8 1 0.402 1 5 3 3 0M77 group Remark: P value is the result a.s compared with the model group.

3. Conclusion

The result of the experiment indicates obvious inhibition effect in the high dose group for acute liver injury caused by CCI4, which is shown by decrease of SGPT and SGOT, relief of degeneration of liver cei I. It also shows effect for declining enzyme and protecting liver to some extent in the medium dose group and the low dose group.

Claims

What is claimed is: 1. A Chinese medicine composition for lowering lipid and protecting liver, being the compound preparation formed by Chinese medicine components and pharmaceutical S adjuvants, characterized in that the Chinese medicine components comprise 60 to 120 parts by weight of Gynura divaricata, 50 to Ii 0 parts by weight of Radix Puerariae and 20 to 60 parts by weight of Radix Paeoniae Alba.
2. Thc Chinese medicine composition according to claim I, characterized in that the Chinese medicine components comprise 80 to 100 parts by weight of Gynura divaricatas, 70 to 90 parts by weight of Radix Pucrariae, and 30 to 50 parts by weight of Radix Paeoniae Alba.
3. The Chinese medicine composition according to claim 1 or 2, characterized in that the Gynura divaricata is the whole herb of Gynura divaricata.
4. The Chinese medicine composition according to claim I or 2, characterized in that the Radix Puerariae is the dried root of Pueraria lobata.
5. The Chinese medicine composition according to claim I or 2, characterized in that the Radix Paconiae Mba is the dried root of Paean/a lact?fiora.
6. The Chinese medicine composition according to claim 1 or 2, characterized in that the compound preparation is in form of capsules, pills, gmnules, or oral liquid.
7. The Chinese medicine composition according to claim 1 or 2, characterized in that Lhe pharmaceutical adjuvant is one of, or a mixture of two or more of dextrin, saccharose. lactose, starch, sodium carhoxymethyl starch, polyvinylpyrrolidone, magnesium stearate.
8. A preparation method of the Chinese medicine composition according to claim 1 or 2, characterized in that the method comprises steps as follows: 1) decoeting Gynura divaricata with water for 1.5 to 2 hours, wherein the weight of water is 8 to 12 times the weight of Gynura divarieata, collecting the extracting solution; then adding water again into the residue thereof, wherein the weight of water is 5 to 8 times the weight of the residue, decoeting them for 1 to 1.5 hours, collecting the extracting solution again; mixing both extracting solutions. filtrating and concentrating it, then adding alcohol thereto and making the content of alcohol in 70% to 90%, filtrating, concentrating and drying the alcohol solution, and obtaining the extractive of Gynura divaricata.

2) decoeting Radix Puerariac with water for 1.5 to 2 hours, wherein the weight of water is 8 to 12 times the weight of Radix Puerariae, collecting the extracting solution; then adding water again into the residue thereof, wherein the weight of water is 5 to 8 times the weight of the residue, decocting them for I to 1.5 hours, collecting the extracting solution again; mixing both extracting solutions, filtrating and concentrating it, then adding alcohol thereto and making the content of alcohol in 70% to 90%. filtrating, concentrating and drying the alcohol solution, and obtaining the extraetive of Radix Puerariae; 3) deeoeting Radix Paeoniae Alba with water for 1.5 to 2 hours, wherein the weight of water is 8 to 12 times the weight of Radix Paeoniae Aiha, collecting the extracting solution; then adding water again into the residue thereof, wherein the weight of water is S to 8 times the weight of the residue, decocting them for Ito 1.5 hours, coliceting the extracting solution again; mixing both extracting solutions, filtrating and concentrating it, then adding alcohol thereto and making the content of alcohol in 70% to 9O% filtrating, concentrating and drying the alcohol solution, and obtaining the extractive of Radix Paeoniae Alba; and 4) mixing the extractives of Gynura divarieata, Radix Puerariae and Radix Paeoniae Alba all together to obtain a total extractive, then adding pharmaceutical adjuvants thereto, mixing them evenly, pelleting and drying them, then pills can be produced by tableting them, or capsules can be produced by packing them into capsules, or granules can be produced by packing them.
9. A preparation method of the Chinese medicine composition according to claim 1 or 2, characterized in that the method comprises steps as follows; I) decoeting GynLLra divaricata with water for 1.5 to 2 hours, wherein the weight of waler is 8 to 12 times the weight of Gynura divaricata, collecting the extracting solution; then adding waler again into the residue thereof; wherein the weight of water is 5 In 8 times the weight of the residue, decocting them for 1 to] .5 hours, collecting the extracting solution again; mixing both extracting solutions, filtrating and concentrating it, then adding alcohol thereto and making the content of alcohol in 70% to 90%, filtrating and concentrating the alcohol solution, and obtaining the extraelive solution of Gynura divaricata; 2) decoeting Radix Puerariae with water for 1.5 to 2 hours, wherein the weight of water is 8 to 12 times the weight of Radix Puerariae, collecting the extracting solution; then adding water again into the residue thereof, wherein the weight of water is 5 to 8 times the weight of the residue, decocting them for 1 to 1.5 hours, collecting the extracting solution again; mixing both extracting solutions, filtrating and concentrating it, then adding alcohol thereto and making the content of alcohol in 70% to 90%, filtraling and concentrating the alcohol solution, and obtaining the extractive solution of Radix Puerariae; 3) decocting Radix Paeoniae Alba with water for 1.5 to 2 hours, wherein the weight of water is 8 to 12 times the weight of Radix Paeoniae Alba, collecting the extracting solution; then adding waler again into the residue thereof, wherein the weight of water is to 8 times the weight of the residue, decocting them for 1 to 1.5 hours, collecting the extracting solulion again; mixing both extracting solutions, filtrating and concentrating it, then adding alcohol thereto and niaking the content of alcohol in 70% to 90%, filtrating and concentrating the alcohol solution, and obtaining the extractive solution of Radix Paeoniae Alba; and 4) mixing the extracting solutions of Gynura divaricata, Radix Puerariae and radixRadix Paeoniae Alba all together, and adding corrective thereto to obtain an oral liquid.
10. Application of the Chinese medicine composition for lowering lipid and protecting liver according to claim I or 2 on preparing medicines and health products for preventing aild treating hyperlipideniia, obesitç viral hepatitis. chemical liver injury, and alcoholic liver in] ury.