GB2516581A - Culture of bacteriophage - Google Patents

Culture of bacteriophage Download PDF

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Publication number
GB2516581A
GB2516581A GB1420253.5A GB201420253A GB2516581A GB 2516581 A GB2516581 A GB 2516581A GB 201420253 A GB201420253 A GB 201420253A GB 2516581 A GB2516581 A GB 2516581A
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GB
United Kingdom
Prior art keywords
bacteriophage
vessel
culture
harvesting
excess
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB1420253.5A
Other versions
GB2516581B (en
GB201420253D0 (en
Inventor
Michael Mattey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fixed Phage Ltd
Original Assignee
Fixed Phage Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fixed Phage Ltd filed Critical Fixed Phage Ltd
Publication of GB201420253D0 publication Critical patent/GB201420253D0/en
Publication of GB2516581A publication Critical patent/GB2516581A/en
Application granted granted Critical
Publication of GB2516581B publication Critical patent/GB2516581B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/26—Inoculator or sampler
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/58—Reaction vessels connected in series or in parallel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/00051—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Clinical Laboratory Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Bacteriophage are produced in a continuous process comprising maintaining a culture of bacteriophage in a first vessel (16); feeding the culture with bacteria maintained in a second vessel (12a, 12b) in the stationary phase and harvesting bacteriophage from the first vessel (16), wherein the first vessel is operated at a dilution rate in excess of 1 / (bacteriophage lysis time).
GB1420253.5A 2012-06-28 2013-06-28 Culture of bacteriophage Active GB2516581B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB201211497A GB201211497D0 (en) 2012-06-28 2012-06-28 Culture of Bacteriophage
PCT/EP2013/063633 WO2014001504A2 (en) 2012-06-28 2013-06-28 Culture of bacteriophage

Publications (3)

Publication Number Publication Date
GB201420253D0 GB201420253D0 (en) 2014-12-31
GB2516581A true GB2516581A (en) 2015-01-28
GB2516581B GB2516581B (en) 2017-03-15

Family

ID=46704368

Family Applications (2)

Application Number Title Priority Date Filing Date
GB201211497A Ceased GB201211497D0 (en) 2012-06-28 2012-06-28 Culture of Bacteriophage
GB1420253.5A Active GB2516581B (en) 2012-06-28 2013-06-28 Culture of bacteriophage

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GB201211497A Ceased GB201211497D0 (en) 2012-06-28 2012-06-28 Culture of Bacteriophage

Country Status (2)

Country Link
GB (2) GB201211497D0 (en)
WO (1) WO2014001504A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2016353541A1 (en) * 2015-11-11 2018-06-21 Elizabeth Jayne ELLIOTT Method and apparatus of improved bacteriophage yield
CN109609392B (en) * 2018-12-24 2022-04-19 上海智城分析仪器制造有限公司 Siphon catheter feeding table cultivation method for yeast
WO2024091978A1 (en) * 2022-10-25 2024-05-02 Sug Biosciences Llc Biocontrol compositions of bacteriophage, methods of producing and uses thereof
CN118222516B (en) * 2024-02-20 2024-08-30 青岛润达生物科技有限公司 Phage RDP-SA-21030 capable of lysing salmonella at stable phase, bactericidal composition and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009044414A1 (en) * 2007-10-02 2009-04-09 Gima S.P.A. Process and system for the industrial scale purification of bacteriophages intended for bacteriophage therapy
US20110014157A1 (en) * 2008-03-20 2011-01-20 Phytoline Gmbh Method for producing a mixture of bacteriophages and the use thereof in the therapy of antibiotic-resistant staphylococci
US20120040329A1 (en) * 2010-08-12 2012-02-16 Phage Biocontrol Research, Llc Process For Continuous Production Of Bacteriophage

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009044414A1 (en) * 2007-10-02 2009-04-09 Gima S.P.A. Process and system for the industrial scale purification of bacteriophages intended for bacteriophage therapy
US20110014157A1 (en) * 2008-03-20 2011-01-20 Phytoline Gmbh Method for producing a mixture of bacteriophages and the use thereof in the therapy of antibiotic-resistant staphylococci
US20120040329A1 (en) * 2010-08-12 2012-02-16 Phage Biocontrol Research, Llc Process For Continuous Production Of Bacteriophage

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LINDEMANN B F ET AL: "Evolution of bacteriophage in continuous culture: A model system to test antiviral gene therapies for the emergence of phage escape mutants", JOURNAL OF VIROLOGY, THE AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 76, no. 11, 1 June 2002, pp 5784-5792, XP002570561. *
SAUVAGEAU DOMINIC ET AL: "Two-stage, self-cycling process for the production of bacteriophages", MICROBIAL CELL FACTORIES, BIOMED CENTRAL, LONDON, 1 November 2010, vol. 9, no. 1, page 81, XP021077229, *

Also Published As

Publication number Publication date
WO2014001504A2 (en) 2014-01-03
WO2014001504A3 (en) 2014-02-20
GB2516581B (en) 2017-03-15
GB201420253D0 (en) 2014-12-31
GB201211497D0 (en) 2012-08-08

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