GB2485862B - Preparation process of L-arginine-alpha-ketoglutarate by direct crystallization from fermentation broth - Google Patents

Preparation process of L-arginine-alpha-ketoglutarate by direct crystallization from fermentation broth

Info

Publication number
GB2485862B
GB2485862B GB1111221.6A GB201111221A GB2485862B GB 2485862 B GB2485862 B GB 2485862B GB 201111221 A GB201111221 A GB 201111221A GB 2485862 B GB2485862 B GB 2485862B
Authority
GB
United Kingdom
Prior art keywords
ketoglutarate
arginine
alpha
preparation process
fermentation broth
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
GB1111221.6A
Other versions
GB201111221D0 (en
GB2485862A (en
Inventor
Shaoping Li
Jiamao Fang
Weibin Chen
Zhenghong Xu
Jinsong Shi
Zhihao Sun
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GUANGDONG HUANXI BIOLOG TECHNOLOGY CO Ltd
Original Assignee
GUANGDONG HUANXI BIOLOG TECHNOLOGY CO Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CN2010105650205A external-priority patent/CN102020593B/en
Application filed by GUANGDONG HUANXI BIOLOG TECHNOLOGY CO Ltd filed Critical GUANGDONG HUANXI BIOLOG TECHNOLOGY CO Ltd
Publication of GB201111221D0 publication Critical patent/GB201111221D0/en
Publication of GB2485862A publication Critical patent/GB2485862A/en
Application granted granted Critical
Publication of GB2485862B publication Critical patent/GB2485862B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/10—Citrulline; Arginine; Ornithine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/14—Glutamic acid; Glutamine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/50—Polycarboxylic acids having keto groups, e.g. 2-ketoglutaric acid

Landscapes

  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

A method of preparing L-arginine-alpha-ketoglutarate (AAKG) is disclosed comprising adding L-arginine and α-ketoglutarate which are obtained as fermentation products from microbes, the method further comprising concentration in a vacuum, crystallisation, dissolution discolouration, re-crystallisation and crystal drying.
GB1111221.6A 2010-11-29 2011-04-11 Preparation process of L-arginine-alpha-ketoglutarate by direct crystallization from fermentation broth Expired - Fee Related GB2485862B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN2010105650205A CN102020593B (en) 2010-11-29 2010-11-29 Process for preparing L-arginine-alpha-ketoglutarate (AAKG) from fermentation liquor through direct crystallization
PCT/CN2011/000629 WO2012071766A1 (en) 2010-11-29 2011-04-11 Preparation method of l-arginine-α-ketoglutarate by direct crystallization from zymotic fluid

Publications (3)

Publication Number Publication Date
GB201111221D0 GB201111221D0 (en) 2011-08-17
GB2485862A GB2485862A (en) 2012-05-30
GB2485862B true GB2485862B (en) 2013-09-04

Family

ID=44511925

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1111221.6A Expired - Fee Related GB2485862B (en) 2010-11-29 2011-04-11 Preparation process of L-arginine-alpha-ketoglutarate by direct crystallization from fermentation broth

Country Status (1)

Country Link
GB (1) GB2485862B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113527413B (en) * 2021-07-29 2023-08-11 山东华辰生物化学有限公司 Antibacterial peptide crude liquid separation and purification device and method
CN115448885A (en) * 2022-10-24 2022-12-09 生工生物工程（上海）股份有限公司 Imidazole purification method

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1441055A (en) * 2003-02-25 2003-09-10 江南大学 L-arginine producing strain and its mutation method and usage in producing L-arginine
CN1544642A (en) * 2003-11-13 2004-11-10 江南大学 Method for microbial fermentation synthesis of alpha- ketoglutaric acid
CN101591271A (en) * 2008-05-27 2009-12-02 上海汉飞生化科技有限公司 A kind of preparation method of crystal L-arginine alpha-ketoglutarate
CN102020593A (en) * 2010-11-29 2011-04-20 广东环西生物科技股份有限公司 Process for preparing L-arginine-alpha-ketoglutarate (AAKG) from fermentation liquor through direct crystallization

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1441055A (en) * 2003-02-25 2003-09-10 江南大学 L-arginine producing strain and its mutation method and usage in producing L-arginine
CN1544642A (en) * 2003-11-13 2004-11-10 江南大学 Method for microbial fermentation synthesis of alpha- ketoglutaric acid
CN101591271A (en) * 2008-05-27 2009-12-02 上海汉飞生化科技有限公司 A kind of preparation method of crystal L-arginine alpha-ketoglutarate
CN102020593A (en) * 2010-11-29 2011-04-20 广东环西生物科技股份有限公司 Process for preparing L-arginine-alpha-ketoglutarate (AAKG) from fermentation liquor through direct crystallization

Also Published As

Publication number Publication date
GB201111221D0 (en) 2011-08-17
GB2485862A (en) 2012-05-30

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20190701