GB2480419B - Method for the manufacture of ethanol - Google Patents

Method for the manufacture of ethanol

Info

Publication number
GB2480419B
GB2480419B GB1116293.0A GB201116293A GB2480419B GB 2480419 B GB2480419 B GB 2480419B GB 201116293 A GB201116293 A GB 201116293A GB 2480419 B GB2480419 B GB 2480419B
Authority
GB
United Kingdom
Prior art keywords
ethanol
manufacture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
GB1116293.0A
Other versions
GB2480419A (en
GB201116293D0 (en
Inventor
Steven Lewis Kelly
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UWS Ventures Ltd
Original Assignee
UWS Ventures Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by UWS Ventures Ltd filed Critical UWS Ventures Ltd
Publication of GB201116293D0 publication Critical patent/GB201116293D0/en
Publication of GB2480419A publication Critical patent/GB2480419A/en
Application granted granted Critical
Publication of GB2480419B publication Critical patent/GB2480419B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0108—Fructan beta-fructosidase (3.2.1.80)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
GB1116293.0A 2009-02-26 2010-02-24 Method for the manufacture of ethanol Expired - Fee Related GB2480419B (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15558909P 2009-02-26 2009-02-26
GB0903224A GB2468134A (en) 2009-02-26 2009-02-26 Yeast for fructan utilisation
PCT/EP2010/052350 WO2010097415A2 (en) 2009-02-26 2010-02-24 Yeast for fructan utilisation

Publications (3)

Publication Number Publication Date
GB201116293D0 GB201116293D0 (en) 2011-11-02
GB2480419A GB2480419A (en) 2011-11-16
GB2480419B true GB2480419B (en) 2014-09-17

Family

ID=40565718

Family Applications (2)

Application Number Title Priority Date Filing Date
GB0903224A Withdrawn GB2468134A (en) 2009-02-26 2009-02-26 Yeast for fructan utilisation
GB1116293.0A Expired - Fee Related GB2480419B (en) 2009-02-26 2010-02-24 Method for the manufacture of ethanol

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GB0903224A Withdrawn GB2468134A (en) 2009-02-26 2009-02-26 Yeast for fructan utilisation

Country Status (2)

Country Link
GB (2) GB2468134A (en)
WO (1) WO2010097415A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2801577A1 (en) 2010-06-03 2011-12-08 Mascoma Corporation Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose
CN115838710B (en) * 2022-12-05 2024-06-18 江苏海洋大学 Exo-levan enzyme capable of degrading levan and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2485339A1 (en) * 1980-06-25 1981-12-31 Beghin Say Sa Prodn. of syrups contg. glucose and fructose - by contacting poly:fructoside(s) with microorganisms
AT398435B (en) * 1992-08-20 1994-12-27 Helmut Dipl Ing Dr Schwab Modified polyfructan-hydrolyzing enzyme
WO2000068402A1 (en) * 1999-05-06 2000-11-16 K.U. Leuven Research & Development Manipulation of fructan catabolism in plants
WO2010097416A1 (en) * 2009-02-26 2010-09-02 Uws Ventures Limited Fructanase

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2161652C1 (en) * 2000-02-08 2001-01-10 Московский Государственный Университет пищевых производств Method of production of ethyl alcohol from jerusalem artichoke
US7314974B2 (en) * 2002-02-21 2008-01-01 Monsanto Technology, Llc Expression of microbial proteins in plants for production of plants with improved properties
JP4592387B2 (en) * 2004-11-02 2010-12-01 株式会社廣貫堂 Inulin-degrading enzyme and its gene
WO2008040358A1 (en) * 2006-10-03 2008-04-10 Protanol Bv A method for providing proteins and fermentation products from a plant material

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2485339A1 (en) * 1980-06-25 1981-12-31 Beghin Say Sa Prodn. of syrups contg. glucose and fructose - by contacting poly:fructoside(s) with microorganisms
AT398435B (en) * 1992-08-20 1994-12-27 Helmut Dipl Ing Dr Schwab Modified polyfructan-hydrolyzing enzyme
WO2000068402A1 (en) * 1999-05-06 2000-11-16 K.U. Leuven Research & Development Manipulation of fructan catabolism in plants
WO2010097416A1 (en) * 2009-02-26 2010-09-02 Uws Ventures Limited Fructanase

Non-Patent Citations (13)

* Cited by examiner, † Cited by third party
Title
Applied and environmental microbiology, Vol 59, 1993, K Ohta et al. "Production of high concentrations of ethanol from inulin by simultaneous saccharification and fermentation using Aspergillus niger and Saccharomyces cerevisiae". pages 729 - 733. *
Applied biochemistry and biotechnology, Vol129-132, 2006, Ma J Negro et al "Inulin-containing biomass for ethanol production: carbohydrate extraction and ethanol fermentation", pages 922 - 932 *
Biotechnology and bioengineering, Vol. 60, 1998, EE Brevnova et al. "Inulase-secreting strain of Saccharomyces cerevisiae produces fructose", pages 492-497 *
Biotechnology letters Vol 26, 2004, K Szambelan et al. "Use of Zymomonas mobilis and Saccharomyces cerevisiae mixed with Kluyveromyces fragilis for improved ethanol production from Jerusalem artichoke tubers", pages 845 - 848 *
DNA, Vol 5, 1986, RF Ernst, "Improved secretion of heterologous proteins...", 483-491 *
FEBS letters, Vol. 289, 1991, O Laloux et al. "Cloning and sequencing of the inulinase gene of Kluyveromyces marxianus var. marxianus ATCC 12424", pages 64 - 68 *
FEMS microbiology letters, Vol. 154, 1997, AN Miasnikov, "Characterization of a novel endo-levanase and its gene from Bacillus sp.L7", pages 23 - 28 *
FEMS Yeast Research, Vol 5, 2005, H Salo et al, "Co-expression of two mammalian glycosyltransferases in the yeast cell wall allows synthesis of sLex", pages 341-350 *
Gene, Vol 161, 1995, Wanker E et al. "Efficient secretion of Bacillus subtilis levanase by Saccharomyces cerevisiae", pages 45 - 49 *
Gene, Vol 79, 1989, S Elliott et al, "Secretion of glycosylated human erythropoietin from yeast directed by the ..."167-180 *
Journal of biotechnology, Vol.18, 1991, E Wanker et al. "Expression of the Bacillus subtilis levanase gene in Escherichia coli and Saccharomyces cerevisiae.", pages 243 - 254 *
Proc. Natl. Acad, Sci. USA, Vol 81, 1984, GA Bitter et al, "Secretion of foreign proteins from Saccharomyces cerevisiae directed by a-factor gene fusions", 5330-5334 *
Systematic and applied microbiology, Vol Paludan-MÜller C. et al. "Purification and characterisation of an extracellular fructan beta-fructosidase from a Lactobacillus pentosus strain isolated from fermented fish", pages 13 - 20 *

Also Published As

Publication number Publication date
GB2480419A (en) 2011-11-16
WO2010097415A2 (en) 2010-09-02
GB0903224D0 (en) 2009-04-08
GB201116293D0 (en) 2011-11-02
WO2010097415A3 (en) 2010-12-02
GB2468134A (en) 2010-09-01

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20210224