GB2480419A - Yeast for fructan utilisation - Google Patents

Yeast for fructan utilisation Download PDF

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Publication number
GB2480419A
GB2480419A GB1116293A GB201116293A GB2480419A GB 2480419 A GB2480419 A GB 2480419A GB 1116293 A GB1116293 A GB 1116293A GB 201116293 A GB201116293 A GB 201116293A GB 2480419 A GB2480419 A GB 2480419A
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United Kingdom
Prior art keywords
yeast
yeast cell
fructan
utilisation
fructans
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GB1116293A
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GB2480419B (en
GB201116293D0 (en
Inventor
Steven Lewis Kelly
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UWS Ventures Ltd
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UWS Ventures Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0108—Fructan beta-fructosidase (3.2.1.80)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to a yeast cell expressing one or more proteins with fructanase activity as well as a synthetic gene and an expression system for its expression in yeast cells. While naturally occurring yeast cells do not have any catabolic or metabolic capabilities to use fructans as a source, the yeast cell according to the invention has a fructanase activity and can break down or hydrolyse fructans into fructose molecules. The yeast cell of the present invention may be used in bioethanol production.

Description

GB 2480419 A continuation (56) cont FEMS microbiology letters, Vol. 154, 1997, AN Miasnikov, "Characterization of a novel endo-levanase and its gene from Bacillus sp.L7", pages 23 -28 Systematic and applied microbiology, Vol Paludan-MUller C. et al. "Purification and characterisation of an extracellular fructan beta-fructosidase from a Lactobacillus pentosus strain isolated from fermented fish", pages 13 -20 Applied biochemistry and biotechnology, Voll 29-132, 2006, Ma J Negro et al "Inulin-containing biomass for ethanol production: carbohydrate extraction and ethanol fermentation", pages 922 -932 Biotechnology letters Vol 26, 2004, K Szambelan et al. "Use of Zymomonas mobilis and Saccharomyces cerevisiae mixed with Kluyveromyces fragilis for improved ethanol production from Jerusalem artichoke tubers", pages 845 -848 Applied and environmental microbiology, Vol 59, 1993, K Ohta et al. "Production of high concentrations of ethanol from inulin by simultaneous saccharification and fermentation using Aspergillus niger and Saccharomyces cerevisiae". pages 729 -733.
(58) Field of Search by ISA:
INT CL CI2N, CI2R Other: ONLINE: EPO-INTERNAL, BIOSIS, CHEM ABS DATA, SEQUENCE SEARCH, EMBASE, WPI DATA
GB1116293.0A 2009-02-26 2010-02-24 Method for the manufacture of ethanol Expired - Fee Related GB2480419B (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15558909P 2009-02-26 2009-02-26
GB0903224A GB2468134A (en) 2009-02-26 2009-02-26 Yeast for fructan utilisation
PCT/EP2010/052350 WO2010097415A2 (en) 2009-02-26 2010-02-24 Yeast for fructan utilisation

Publications (3)

Publication Number Publication Date
GB201116293D0 GB201116293D0 (en) 2011-11-02
GB2480419A true GB2480419A (en) 2011-11-16
GB2480419B GB2480419B (en) 2014-09-17

Family

ID=40565718

Family Applications (2)

Application Number Title Priority Date Filing Date
GB0903224A Withdrawn GB2468134A (en) 2009-02-26 2009-02-26 Yeast for fructan utilisation
GB1116293.0A Expired - Fee Related GB2480419B (en) 2009-02-26 2010-02-24 Method for the manufacture of ethanol

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GB0903224A Withdrawn GB2468134A (en) 2009-02-26 2009-02-26 Yeast for fructan utilisation

Country Status (2)

Country Link
GB (2) GB2468134A (en)
WO (1) WO2010097415A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011153516A2 (en) 2010-06-03 2011-12-08 Mascoma Corporation Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2485339A1 (en) * 1980-06-25 1981-12-31 Beghin Say Sa Prodn. of syrups contg. glucose and fructose - by contacting poly:fructoside(s) with microorganisms
AT398435B (en) * 1992-08-20 1994-12-27 Helmut Dipl Ing Dr Schwab Modified polyfructan-hydrolyzing enzyme
WO2000068402A1 (en) * 1999-05-06 2000-11-16 K.U. Leuven Research & Development Manipulation of fructan catabolism in plants
WO2010097416A1 (en) * 2009-02-26 2010-09-02 Uws Ventures Limited Fructanase

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2161652C1 (en) * 2000-02-08 2001-01-10 Московский Государственный Университет пищевых производств Method of production of ethyl alcohol from jerusalem artichoke
US7314974B2 (en) * 2002-02-21 2008-01-01 Monsanto Technology, Llc Expression of microbial proteins in plants for production of plants with improved properties
JP4592387B2 (en) * 2004-11-02 2010-12-01 株式会社廣貫堂 Inulin-degrading enzyme and its gene
WO2008040358A1 (en) * 2006-10-03 2008-04-10 Protanol Bv A method for providing proteins and fermentation products from a plant material

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2485339A1 (en) * 1980-06-25 1981-12-31 Beghin Say Sa Prodn. of syrups contg. glucose and fructose - by contacting poly:fructoside(s) with microorganisms
AT398435B (en) * 1992-08-20 1994-12-27 Helmut Dipl Ing Dr Schwab Modified polyfructan-hydrolyzing enzyme
WO2000068402A1 (en) * 1999-05-06 2000-11-16 K.U. Leuven Research & Development Manipulation of fructan catabolism in plants
WO2010097416A1 (en) * 2009-02-26 2010-09-02 Uws Ventures Limited Fructanase

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
Applied and environmental microbiology, Vol 59, 1993, K Ohta et al. "Production of high concentrations of ethanol from inulin by simultaneous saccharification and fermentation using Aspergillus niger and Saccharomyces cerevisiae". pages 729 - 733. *
Applied biochemistry and biotechnology, Vol129-132, 2006, Ma J Negro et al "Inulin-containing biomass for ethanol production: carbohydrate extraction and ethanol fermentation", pages 922 - 932 *
Biotechnology and bioengineering, Vol. 60, 1998, EE Brevnova et al. "Inulase-secreting strain of Saccharomyces cerevisiae produces fructose", pages 492-497 *
Biotechnology letters Vol 26, 2004, K Szambelan et al. "Use of Zymomonas mobilis and Saccharomyces cerevisiae mixed with Kluyveromyces fragilis for improved ethanol production from Jerusalem artichoke tubers", pages 845 - 848 *
FEBS letters, Vol. 289, 1991, O Laloux et al. "Cloning and sequencing of the inulinase gene of Kluyveromyces marxianus var. marxianus ATCC 12424", pages 64 - 68 *
FEMS microbiology letters, Vol. 154, 1997, AN Miasnikov, "Characterization of a novel endo-levanase and its gene from Bacillus sp.L7", pages 23 - 28 *
Gene, Vol 161, 1995, Wanker E et al. "Efficient secretion of Bacillus subtilis levanase by Saccharomyces cerevisiae", pages 45 - 49 *
Journal of biotechnology, Vol.18, 1991, E Wanker et al. "Expression of the Bacillus subtilis levanase gene in Escherichia coli and Saccharomyces cerevisiae.", pages 243 - 254 *
Systematic and applied microbiology, Vol Paludan-M©ller C. et al. "Purification and characterisation of an extracellular fructan beta-fructosidase from a Lactobacillus pentosus strain isolated from fermented fish", pages 13 - 20 *

Also Published As

Publication number Publication date
GB2480419B (en) 2014-09-17
GB2468134A (en) 2010-09-01
WO2010097415A3 (en) 2010-12-02
GB0903224D0 (en) 2009-04-08
WO2010097415A2 (en) 2010-09-02
GB201116293D0 (en) 2011-11-02

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PCNP Patent ceased through non-payment of renewal fee

Effective date: 20210224