GB2478682A - A method fpr producing a hybrid seed using plant of novel cytoplasmic-genic male sterility raphanus sativus line and dna markers for selecting the plant of sa - Google Patents

A method fpr producing a hybrid seed using plant of novel cytoplasmic-genic male sterility raphanus sativus line and dna markers for selecting the plant of sa Download PDF

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Publication number
GB2478682A
GB2478682A GB1111840A GB201111840A GB2478682A GB 2478682 A GB2478682 A GB 2478682A GB 1111840 A GB1111840 A GB 1111840A GB 201111840 A GB201111840 A GB 201111840A GB 2478682 A GB2478682 A GB 2478682A
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GB
United Kingdom
Prior art keywords
plant
raphanus sativus
cgms
sativus line
markers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB1111840A
Other versions
GB201111840D0 (en
GB2478682B (en
Inventor
Soon Kee Sung
Sunggil Kim
Young Soon Ahn
Hyo Jeong Kim
Chae Wan Lim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DB HiTek Co Ltd
Original Assignee
Dongbu HitekCo Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dongbu HitekCo Ltd filed Critical Dongbu HitekCo Ltd
Priority claimed from PCT/KR2008/001935 external-priority patent/WO2008123714A1/en
Publication of GB201111840D0 publication Critical patent/GB201111840D0/en
Publication of GB2478682A publication Critical patent/GB2478682A/en
Application granted granted Critical
Publication of GB2478682B publication Critical patent/GB2478682B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10Seeds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/13Plant traits

Abstract

The present invention relates to a method for producing hybrid seeds using a novel Cytoplasmic-Genic. Male Sterility (CGMS) Raphanus sativus line plant and DNA markers for the selection of the same, more precisely a novel CGMS (D-CGMS) Raphanus sativus line plant, a method for producing hybrid seeds using the same, chloroplast DNA-based markers for the selection of the D-CGMS Raphanus sativus line plant having the nucleotide sequence represented by SEQ. ID. NO: 5, mitochondrial DNA-based SNP (Single Nucleotide Polymorphism) markers for the selection of the D-CGMS Raphanus sativus line plant located on the 171st nucleotide of the nucleotide sequence represented by SEQ. ID. NO: 12. The D-CGMS Raphanus sativus line plant of the present invention can be effectively used for the production of hybrid seeds owing to its high stability of male sterility. The chloroplast DNA-based markers and mitochondrial DNA-based SNP markers for the selection of D-CGMS Raphanus sativus line plants can be very effective in the selection of D-CGMS Raphanus sativus line plants during breeding process.
GB1111840.3A 2007-04-06 2008-04-04 A method for producing a hybrid seed using plant of novel cytoplasmic-genic male sterility raphanus sativus line and dna markers for selecting the plant... Expired - Fee Related GB2478682B (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR20070033962 2007-04-06
PCT/KR2008/001935 WO2008123714A1 (en) 2007-04-06 2008-04-04 A method for producing a hybrid seed using plant of novel cytoplasmic-genic male sterility raphanus sativus line and dna markers for selecting the plant of said raphanus sativus line
KR1020080031591A KR100885075B1 (en) 2007-04-06 2008-04-04 A Method for producing a hybrid seed using plant of novel Cytoplasmic?Genic Male Sterility Raphanus sativus line and DNA markers for selecting the plant of said Raphanus sativus line

Publications (3)

Publication Number Publication Date
GB201111840D0 GB201111840D0 (en) 2011-08-24
GB2478682A true GB2478682A (en) 2011-09-14
GB2478682B GB2478682B (en) 2012-10-24

Family

ID=40151975

Family Applications (2)

Application Number Title Priority Date Filing Date
GB1111840.3A Expired - Fee Related GB2478682B (en) 2007-04-06 2008-04-04 A method for producing a hybrid seed using plant of novel cytoplasmic-genic male sterility raphanus sativus line and dna markers for selecting the plant...
GBGB1017160.1A Pending GB201017160D0 (en) 2007-04-06 2010-10-05 A method for producing a hybrid seed using plant of novel cytoplasmic-genic male sterility raphanus sativus line and DNA markers for selecting the plant

Family Applications After (1)

Application Number Title Priority Date Filing Date
GBGB1017160.1A Pending GB201017160D0 (en) 2007-04-06 2010-10-05 A method for producing a hybrid seed using plant of novel cytoplasmic-genic male sterility raphanus sativus line and DNA markers for selecting the plant

Country Status (5)

Country Link
JP (1) JP5089764B2 (en)
KR (1) KR100885075B1 (en)
CN (1) CN101679982B (en)
CA (1) CA2720710A1 (en)
GB (2) GB2478682B (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107828909A (en) * 2017-11-15 2018-03-23 云南省农业科学院花卉研究所 A kind of male sterile method of Markers for Detection oriental hybrid lily special group
KR102169147B1 (en) * 2018-12-11 2020-10-22 대한민국 A Set Of Single Nucleotide Polymerphism Probe For Backcross Analysis Or Distinction Of Raphanus Sativus
KR20210153071A (en) 2019-04-17 2021-12-16 가부시키가이샤 사카타노타네 Cytoplasmic male sterility Brassica rapa plants with improved growth
TW202107981A (en) 2019-04-17 2021-03-01 日商莎卡達種子股份有限公司 Cms lectuca species plant modified in low temperature growth
KR102448347B1 (en) * 2020-10-27 2022-09-28 전남대학교 산학협력단 Method for determining male-sterility of Raphanus sativus and Raphanus sativus plant having male-sterility
CN112535099B (en) * 2020-12-04 2022-07-08 四川省农业科学院水稻高粱研究所 Breeding and utilizing method of black radish germplasm resources
CN114027173A (en) * 2021-11-16 2022-02-11 山西农业大学 Vegetable male sterile line hybrid seed production method

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06343359A (en) * 1992-10-15 1994-12-20 Mitsubishi Corp Preparation of first filial hybrid variety of rape
KR100399333B1 (en) * 2002-10-10 2003-09-26 Nong Woo Bio Co Ltd Novel genotype cms radish family plant, method for producing hybrid seeds using the same, and dna marker for selecting the nwb-cms radish family plant

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3478953B2 (en) * 1997-09-03 2003-12-15 Necエレクトロニクス株式会社 Semiconductor storage device

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06343359A (en) * 1992-10-15 1994-12-20 Mitsubishi Corp Preparation of first filial hybrid variety of rape
KR100399333B1 (en) * 2002-10-10 2003-09-26 Nong Woo Bio Co Ltd Novel genotype cms radish family plant, method for producing hybrid seeds using the same, and dna marker for selecting the nwb-cms radish family plant

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GenBank Accession AF451565 (27/06/2002) *
GenBank Accession S46795 (08/05/1993) *
Theor. Appl. Genet., Vol.111, 2005, Nahm, S. H. et al., "Development of a molecular marker..", pp.1191-1200 *

Also Published As

Publication number Publication date
KR100885075B1 (en) 2009-02-25
CA2720710A1 (en) 2008-10-16
KR20080091021A (en) 2008-10-09
JP5089764B2 (en) 2012-12-05
GB201111840D0 (en) 2011-08-24
JP2010523106A (en) 2010-07-15
CN101679982A (en) 2010-03-24
CN101679982B (en) 2014-07-23
GB2478682B (en) 2012-10-24
GB201017160D0 (en) 2010-11-24

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PCNP Patent ceased through non-payment of renewal fee

Effective date: 20130404