GB2460977B - Recombinant mammalian cell for screening a substance preventing and treating aids and screening method using the same - Google Patents

Recombinant mammalian cell for screening a substance preventing and treating aids and screening method using the same

Info

Publication number
GB2460977B
GB2460977B GB0916740A GB0916740A GB2460977B GB 2460977 B GB2460977 B GB 2460977B GB 0916740 A GB0916740 A GB 0916740A GB 0916740 A GB0916740 A GB 0916740A GB 2460977 B GB2460977 B GB 2460977B
Authority
GB
United Kingdom
Prior art keywords
screening
same
mammalian cell
recombinant mammalian
treating aids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
GB0916740A
Other versions
GB2460977A (en
GB0916740D0 (en
Inventor
Ji-Chang You
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Avixgen Inc
Original Assignee
Avixgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Avixgen Inc filed Critical Avixgen Inc
Priority claimed from PCT/KR2008/001074 external-priority patent/WO2008105604A1/en
Publication of GB0916740D0 publication Critical patent/GB0916740D0/en
Publication of GB2460977A publication Critical patent/GB2460977A/en
Application granted granted Critical
Publication of GB2460977B publication Critical patent/GB2460977B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983Viruses
    • G01N33/56988HIV or HTLV
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00Use of cells in diagnostics
    • C12N2503/02Drug screening
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00Reverse transcribing RNA viruses
    • C12N2740/00011Details
    • C12N2740/10011Retroviridae
    • C12N2740/16011Human Immunodeficiency Virus, HIV
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00Methods of screening libraries
    • C40B30/04Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00Methods of screening libraries
    • C40B30/06Methods of screening libraries by measuring effects on living organisms, tissues or cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00Detection or diagnosis of diseases
    • G01N2800/24Immunology or allergic disorders

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • AIDS & HIV (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
GB0916740A 2007-02-27 2008-02-25 Recombinant mammalian cell for screening a substance preventing and treating aids and screening method using the same Expired - Fee Related GB2460977B (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR20070019328 2007-02-27
KR1020080013918A KR100987004B1 (en) 2007-02-27 2008-02-15 Recombinant mammalian cell for screening a substance preventing and treating AIDS and screening method using the same
PCT/KR2008/001074 WO2008105604A1 (en) 2007-02-27 2008-02-25 Recombinant mammalian cell for screening a substance preventing and treating aids and screening method using the same

Publications (3)

Publication Number Publication Date
GB0916740D0 GB0916740D0 (en) 2009-11-04
GB2460977A GB2460977A (en) 2009-12-23
GB2460977B true GB2460977B (en) 2011-11-02

Family

ID=40020481

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0916740A Expired - Fee Related GB2460977B (en) 2007-02-27 2008-02-25 Recombinant mammalian cell for screening a substance preventing and treating aids and screening method using the same

Country Status (2)

Country Link
KR (1) KR100987004B1 (en)
GB (1) GB2460977B (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030138954A1 (en) * 2001-10-02 2003-07-24 Didier Trono Methods and compositions relating to restricted expression lentiviral vectors and their applications
KR20040034278A (en) * 2002-10-21 2004-04-28 (주) 에빅스젠 Process for Preparing Nucleocapsid Protein of HIV in Mammalian Cell
KR20060011602A (en) * 2004-07-30 2006-02-03 유지창 Nc mutants having an enhanced binding activity to hiv psi and pharmaceutical composition comprising the same
US7033596B1 (en) * 2000-04-08 2006-04-25 Ji-Chang You Transformant for screening of inhibitors for human immunodeficiency virus

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100599455B1 (en) * 2003-08-30 2006-07-12 유지창 A Transformant for Screening HIV Harboring Chromosome Integrated with Foreign DNA
KR20060000525A (en) * 2004-06-29 2006-01-06 현대자동차주식회사 Propeller shaft structure of a vehicle

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7033596B1 (en) * 2000-04-08 2006-04-25 Ji-Chang You Transformant for screening of inhibitors for human immunodeficiency virus
US20030138954A1 (en) * 2001-10-02 2003-07-24 Didier Trono Methods and compositions relating to restricted expression lentiviral vectors and their applications
KR20040034278A (en) * 2002-10-21 2004-04-28 (주) 에빅스젠 Process for Preparing Nucleocapsid Protein of HIV in Mammalian Cell
KR20060011602A (en) * 2004-07-30 2006-02-03 유지창 Nc mutants having an enhanced binding activity to hiv psi and pharmaceutical composition comprising the same

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Journal of Microbiology and Biotechnology 17 (2007) Park et al 'Expression profiles and pathway analysis in HEK 293 T cells overexpressing HIV-1 tat and nucleocapsid using cDNA microarray' 154-161 *

Also Published As

Publication number Publication date
KR100987004B1 (en) 2010-10-11
KR20080079592A (en) 2008-09-01
GB2460977A (en) 2009-12-23
GB0916740D0 (en) 2009-11-04

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20140225