GB2460265A - Detection assembly - Google Patents
Detection assembly Download PDFInfo
- Publication number
- GB2460265A GB2460265A GB0809364A GB0809364A GB2460265A GB 2460265 A GB2460265 A GB 2460265A GB 0809364 A GB0809364 A GB 0809364A GB 0809364 A GB0809364 A GB 0809364A GB 2460265 A GB2460265 A GB 2460265A
- Authority
- GB
- United Kingdom
- Prior art keywords
- optical
- detector
- coupler
- constituent
- detection assembly
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000001514 detection method Methods 0.000 title abstract description 44
- 230000003287 optical effect Effects 0.000 abstract description 46
- 239000000470 constituent Substances 0.000 abstract description 30
- 230000005540 biological transmission Effects 0.000 abstract description 12
- 230000008878 coupling Effects 0.000 abstract description 4
- 238000010168 coupling process Methods 0.000 abstract description 4
- 238000005859 coupling reaction Methods 0.000 abstract description 4
- 239000013307 optical fiber Substances 0.000 description 11
- 230000002238 attenuated effect Effects 0.000 description 4
- 238000004891 communication Methods 0.000 description 3
- 230000000712 assembly Effects 0.000 description 2
- 238000000429 assembly Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000001917 fluorescence detection Methods 0.000 description 2
- 238000005755 formation reaction Methods 0.000 description 2
- 230000001154 acute effect Effects 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 239000004411 aluminium Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000012777 electrically insulating material Substances 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/05—Flow-through cuvettes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/255—Details, e.g. use of specially adapted sources, lighting or optical systems
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
- G01N27/023—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance where the material is placed in the field of a coil
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
- G02B6/24—Coupling light guides
- G02B6/26—Optical coupling means
- G02B6/262—Optical details of coupling light into, or out of, or between fibre ends, e.g. special fibre end shapes or associated optical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N2021/0346—Capillary cells; Microcells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6484—Optical fibres
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/02—Mechanical
- G01N2201/022—Casings
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
A detection assembly 100 for detecting a characteristic of a constituent comprises a coupler 105 for coupling a pair of optical transmission lines such as optical fibres 108, 110 in registration for facilitating optical transmission of an input optical signal between an optical source 125 and an optical detector 130, such as a spectrophotometric detector. A receiver is provided on the coupler by providing openings 115 for accommodating an input line such as transparent capillary 118 intermediate the optical transmission lines for inputting the constituent to the coupler such that the input optical signal is applied to the constituent. A fluorimetric detector (160, figure 1) and an electrical detector (146, figure 1), such as a capacitively coupled contactless conductivity detector may also be incorporated as part of the detection assembly.
Description
A detection assembly
Field of the Invention
The present invention relates to a detection assembly for detecting a characteristic of a constituent. The present invention more particularly relates to a detection assembly which includes a coupler which is configured for coupling a pair of optical transmission lines in registration for facilitating optical transmission therebetween and for accommodating an input line intermediate the optical transmission lines for providing a constituent for detection.
Background
Detectors for detecting characteristics of constituents are known in the art.
The most commonly used detectors are spectro-photometric detection (SD), fluorescence detection (FD) and capacitively coupled contactless conductivity detection (C4D).Detector assemblies comprising two detectors are known.
Detector assemblies of the type known heretofore are difficult to assemble and typically require a skilled operator to assemble and operate.
There is therefore a need for a detection assembly which is easy to assemble and operate.
These and other features will be better understood with reference to the followings Figures which are provided to assist in an understanding of the teaching of the invention.
Sum mary These and other problems are addressed by providing a detection assembly for detecting a characteristic of a constituent which includes a coupler which is configured for coupling a pair of optical transmission lines in registration for facilitating optical transmission and for accommodating an input line intermediate the optical transmission lines for providing a constituent for detection.
Accordingly, a first embodiment of the invention provides a detection assembly as detailed in claim 1. The invention also provides a system as detailed in claim 21. Advantageous embodiments are provided in the dependent claims.
These and other features will be better understood with reference to the followings Figures which are provided to assist in an understanding of the teaching of the invention.
Brief Description Of The Drawings
The present invention will now be described with reference to the accompanying drawings in which: Figure 1 is a perspective view of a detection assembly for detecting a characteristic of a constituent in accordance with the present invention.
Figure 2 is a perspective view of a portion of the detection assembly of Figure 1.
Figure 3 is a perspective view of a portion of the detection assembly of Figure 1.
Figure 4 is an exploded perspective view of the detection assembly of Figure 1.
Figure 5 is an exploded perspective view of the detection assembly of Figure 1.
Figure 6 is a perspective view of the detection assembly of Figure 1 from plan view.
Figure 7 is a perspective view of the detection assembly of Figure 1 from side view.
Figure 8 is a graph showing three characteristics of a constituent detected simultaneously using the detection assembly of Figure 1.
Detailed Description Of The Drawings
The invention will now be described with reference to an exemplary embodiment which is provided to assist in an understanding of the teaching of the invention.
Referring to the drawings there is provided a detection assembly 100 for detecting a characteristic of a constituent. The constituent may be a liquid or gas. The assembly 100 comprises an elongated cylindrical tubular coupler 105 for coupling a pair of spaced apart optical transmission lines, namely, an input optical fibre 108 and an output optical fibre 110. Once the two optical lines are received within the coupler and are in registration optical transmission therebetween is facilitated. These optical lines are typically received at opposite ends of the coupler. Once received and aligned within the coupler each of the optical lines terminate within the coupler such that a space intermediate each of the lines is defined within the volume or interior region of the coupler 105. A receiver is formed on the coupler 105 by providing a pair of spaced apart openings 115 thereon for accommodating an input line, in this case, a transparent capillary 118. The capillary 118, on receipt through the openings which are provided on an outer surface of the coupler, extends through the openings 115 intermediate the input and output optical fibres 108, 110 and operably is useful for providing the constituent to be analysed. The constituent is urged along the capillary 118 and into a hollow region 122 of the coupler 105 proximal to the space defined between each of the optical fibres 108, 110. An optical source, in this case, an light emitting diode (LED) 125 provides an optical input signal which is guided by the input optical fibre 108 to the constituent located in the hollow region 122 of the coupler 105. The output optical fibre 110 is in optical communication with an optical detector 130 for guiding the optical input signal after it penetrates the constituent for determining its characteristics. It will be appreciated by those skilled in the art that the optical detector may be any desirable optical detector such as a spectrophotometric detector which may be used for monitoring light absorption. The coupler 105 defines a major longitudinal axis 133 and a minor transverse axis 136. The input and optical fibres 108, 110 are arranged to be substantially coaxial with the longitudinal axis 133. The capillary 118 is arranged to be offset from the major longitudinal axis and in this exemplary arrangement is substantially coaxial with the transverse axis 136. The coupler comprises a pair of spaced apart planar ends with an arcuate surface extending therebetween.
The coupler 105 may be provided in a two part construction in which case it comprises a first connecting means associated with the input optical fibres 108, and a second connecting means associated with the output optical fibres 110. The first and second connecting means each comprise a pair of complimentary interengagable formations. The complimentary interengagable formations are provided by plug and socket arrangements. The respective opposite ends of the coupler 105 define corresponding sockets 140 thereby providing female connecting means. The input and output optical fibres 108 and 110 terminate in corresponding plugs 143 which are axially inserted into the respective sockets 140 thereby providing male connecting means. A screw fastening member (not shown) is carried by each of the optical fibres 108, 110 for releasably securing the plugs 143 in the corresponding sockets 140.
Threads are provided adjacent the respective opposite ends of the coupler 105 for mating with corresponding threads on the fastening members.
An electrical detector, namely, a capacitively coupled contactless conductivity detector (C4D) 146 may be incorporated as part of the detection assembly 100 for facilitating detecting an electrical characteristic of the constituent. The C4D detector 146 comprises a transmitting electrode 148 and a spaced apart receiving electrode 150 located at respective opposite sides of the coupler 105. The transmitting electrode 148 is used to subject the constituent in the capillary 118 between the respective electrodes 148, 150 to a large amplitude high frequency electromagnetic signal. The receiving electrode 150 receives a corresponding attenuated AC signal, the level of attenuation being related to the specifics of the material being analysed. A portion of the capillary 118 located in the hollow interior region 122 of the coupler 105 defines a detection area 152. The size of the attenuated signal received by the receiver electrode 150 is affected by the conductivity of the constituent in the capillary 118 in the detection area 152. The electrodes 148, 150 are provided in this arrangement as tubular members which surround or encapsulate corresponding spaced apart portions of the capillary 118 externally of the hollow interior region 122 of the coupler 105. The provision of tubular electrodes 148, 150 allows for easy capillary placement, replacement and repositioning. The coupler 105 is of a metallic material, in this exemplary arrangement, aluminium and is dimensioned to be located between the transmitting and receiving electrodes 148, 150 thereby providing a faraday shield intermediate the transmitting electrode 148 and the receiving electrode 150. A gap is provided between the electrodes 148, 150 and the metallic coupler 105 such that the electrodes 148, are electrically isolated from each other. A supporting means, in this case, a disc 155 extends radially from the coupler 105 for supporting the electrodes 148, 150. The disc 155 comprises an upper disc element 156 and a lower disc 157 element which together define a housing for accommodating the electrodes 148, 150 and a portion of the capillary 118 therein. The disc 155 includes an electrically insulating material for electrically insulating the electrodes 148, 150 from each other.
The optical input signal from the LED 125 may excite the constituent resulting in an optical output signal representative of fluorescent emission. A pick-up optical transmission line, in this case, provided by an optical fibre 158 may be provided in optical communication with the detection area 152 for guiding the optical output signal to a fluorimetric detector 160. The fluorimetric detector 160 performs fluorimetric detection, in a manner which will be known to those skilled in the art. A window 163 is formed on the coupler 105 so that the optical output signal generated in the detection area 152 is transmitted to the fluorescence detector 160 via the pick-up optical fibre 158. The pick-up optical fibre 158 extends radially from the coupler 105 and is offset from the capillary 118. In this exemplary arrangement the capillary 118 is substantially perpendicular to the longitudinal axis 133 of the coupler 105 and defines an acute angle, in this case, 40° with the transverse axis 136. A portion of the pick-up optical fibre 158 is also encapsulated by the disc 155.
In operation, the detection assembly 100 is used to provide inputs to the C4D detector 146, the fluorimetric detector 160 and the spectrophotometric detector 130. The three detectors are in communication with the detection assembly 100 and can be operated individually, in pairs or simultaneously for detecting corresponding characteristic of the constituent in the detection area 152. The fluorimetric detector 160 and the spectrophotometric detector 130 require an optical input signal which is applied to the constituent in the capillary 118 in the detection area 152. The optical input signal is provided by the LED which is then guided to the detection area 152 by the input optical fibre 108.
The input optical signal propagates through the constituent in the capillary 118 in detection area 152. The output optical fibre 110 guides the input optical signal after it has passed through the constituent to the spectrophotometric detector which monitors the amount of light absorbed by the constituent.
The input optical signal excites the constituent in the detection area 152 resulting in an optical output signal representative of fluorescent emission. The pickup optical fibre 158 guides the optical output signal to the fluorimetric detector. The flourimetric detector 160 reads the output signal for performing flourimetric detection.
The C4D detector 146 is operated for detecting the conductivity of the constituent in the capillary 118 between the transmitting electrode 148 and the receiving electrode 150. The transmitting electrode 148 is used to subject the constituent to a large amplitude high frequency electromagnetic signal. The receiving electrode 150 receives a corresponding attenuated AC signal. The size of the attenuated signal received by the receiver electrode 150 is affected by the conductivity of the constituent in the capillary 118 in the detection area 152.
Referring now to the graph of Figure 8 which shows the results from the three detectors 130, 146, 160 which are operably coupled to the detection assembly 100 and are operated simultaneously. The spectrophotometric detector 130 provides photometric detection. The C4D detector 146 provides conductivity detection. The flourimetric detector 160 provides flourimetric detection. It will be appreciated by those skilled in the art that in this arrangement that each of the three detections occur simultaneously. Thus a single point can be detected simultaneously using three discrete detection methods.
It will be understood that what has been described herein is an exemplary embodiment of a detection assembly for detecting a characteristic of a constituent. While the present invention has been described with reference to an exemplary arrangement it will be understood that it is not intended to limit the teaching of the present invention to such arrangements as modifications can be made without departing from the spirit and scope of the present invention. In this way it will be understood that the invention is to be limited only insofar as is deemed necessary in the light of the appended claims.
Similarly the words comprises/comprising when used in the specification are used to specify the presence of stated features, integers, steps or components but do not preclude the presence or addition of one or more additional features, integers, steps, components or groups thereof.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0809364A GB2460265A (en) | 2008-05-23 | 2008-05-23 | Detection assembly |
| PCT/EP2009/056237 WO2009141431A1 (en) | 2008-05-23 | 2009-05-22 | A detection assembly for facilitating simultaneous optical and electrical detection of characteristics of a constituent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0809364A GB2460265A (en) | 2008-05-23 | 2008-05-23 | Detection assembly |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| GB0809364D0 GB0809364D0 (en) | 2008-07-02 |
| GB2460265A true GB2460265A (en) | 2009-11-25 |
Family
ID=39615955
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB0809364A Withdrawn GB2460265A (en) | 2008-05-23 | 2008-05-23 | Detection assembly |
Country Status (2)
| Country | Link |
|---|---|
| GB (1) | GB2460265A (en) |
| WO (1) | WO2009141431A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019206779A1 (en) * | 2018-04-26 | 2019-10-31 | Research Center Pharmaceutical Engineering Gmbh | Monitoring a property of a fluid during a flow process |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8525533B2 (en) | 2010-09-16 | 2013-09-03 | Fresenius Medical Care Holdings, Inc. | Conductivity detector for fluids |
| EP4087858A1 (en) * | 2020-01-10 | 2022-11-16 | Carogen Corporation | Compositions and methods of use of oncolytic virus like vesicles |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992013258A1 (en) * | 1991-01-22 | 1992-08-06 | United States Department Of Energy | On-line process control monitoring system |
| US5442437A (en) * | 1993-09-13 | 1995-08-15 | Atlantic Richfield Company | Sample cell and probe for spectrophotometer |
| WO2000028322A1 (en) * | 1998-11-06 | 2000-05-18 | Medtronic Avecor Cardiovascular, Inc. | Apparatus and method to determine blood parameters |
| DE19905983A1 (en) * | 1999-02-12 | 2000-10-05 | J & M Analytische Mess & Regeltechnik Gmbh | Capillary holder used e.g. in micro-high performance HPLC has inlet and outlet waveguide parts fixed to a substrate |
| US6266139B1 (en) * | 1996-04-23 | 2001-07-24 | Joachim Mannhardt | Capillary tube holder |
| JP2003098091A (en) * | 2001-09-26 | 2003-04-03 | Japan Science & Technology Corp | Reaction pursuing and analyzing device for high-speed ultraviolet-visible light absorption photometry for high- temperature high-pressure solution reaction |
| WO2003034045A1 (en) * | 2001-10-16 | 2003-04-24 | Abb Bomem Inc. | On-line optical analysis of a substance through a conduit section of a process line |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9422392D0 (en) * | 1994-11-05 | 1995-01-04 | Cognitive Solutions Ltd | Detector for chemical analysis |
| US20070077177A1 (en) * | 2005-03-16 | 2007-04-05 | Klaus Witt | Stimulated detection of sample compounds |
-
2008
- 2008-05-23 GB GB0809364A patent/GB2460265A/en not_active Withdrawn
-
2009
- 2009-05-22 WO PCT/EP2009/056237 patent/WO2009141431A1/en active Application Filing
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992013258A1 (en) * | 1991-01-22 | 1992-08-06 | United States Department Of Energy | On-line process control monitoring system |
| US5442437A (en) * | 1993-09-13 | 1995-08-15 | Atlantic Richfield Company | Sample cell and probe for spectrophotometer |
| US6266139B1 (en) * | 1996-04-23 | 2001-07-24 | Joachim Mannhardt | Capillary tube holder |
| WO2000028322A1 (en) * | 1998-11-06 | 2000-05-18 | Medtronic Avecor Cardiovascular, Inc. | Apparatus and method to determine blood parameters |
| DE19905983A1 (en) * | 1999-02-12 | 2000-10-05 | J & M Analytische Mess & Regeltechnik Gmbh | Capillary holder used e.g. in micro-high performance HPLC has inlet and outlet waveguide parts fixed to a substrate |
| JP2003098091A (en) * | 2001-09-26 | 2003-04-03 | Japan Science & Technology Corp | Reaction pursuing and analyzing device for high-speed ultraviolet-visible light absorption photometry for high- temperature high-pressure solution reaction |
| WO2003034045A1 (en) * | 2001-10-16 | 2003-04-24 | Abb Bomem Inc. | On-line optical analysis of a substance through a conduit section of a process line |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019206779A1 (en) * | 2018-04-26 | 2019-10-31 | Research Center Pharmaceutical Engineering Gmbh | Monitoring a property of a fluid during a flow process |
Also Published As
| Publication number | Publication date |
|---|---|
| GB0809364D0 (en) | 2008-07-02 |
| WO2009141431A1 (en) | 2009-11-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| WAP | Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1) |