GB2445488A - System for spore detection - Google Patents

System for spore detection Download PDF

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Publication number
GB2445488A
GB2445488A GB0805032A GB0805032A GB2445488A GB 2445488 A GB2445488 A GB 2445488A GB 0805032 A GB0805032 A GB 0805032A GB 0805032 A GB0805032 A GB 0805032A GB 2445488 A GB2445488 A GB 2445488A
Authority
GB
United Kingdom
Prior art keywords
spores
sample
fluorescence
spore detection
spore
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB0805032A
Other versions
GB2445488B (en
GB0805032D0 (en
Inventor
Lou Reinisch
Sivananthan Sarasanandarajah
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
VERITIDE Ltd
Original Assignee
VERITIDE Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by VERITIDE Ltd filed Critical VERITIDE Ltd
Publication of GB0805032D0 publication Critical patent/GB0805032D0/en
Publication of GB2445488A publication Critical patent/GB2445488A/en
Application granted granted Critical
Publication of GB2445488B publication Critical patent/GB2445488B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A method for detecting the presence of bacterial spores in a sample comprises non-destructively to the spores carrying out the steps of assessing the fluorescence of the sample, subjecting the sample to UV radiation, and reassessing the fluorescence of the sample to determine the presence of spores. An increase in fluorescence indicates the presence of spores.
GB0805032A 2005-09-05 2006-09-05 System for spore detection Expired - Fee Related GB2445488B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NZ542230A NZ542230A (en) 2005-09-05 2005-09-05 System for spore detection
PCT/NZ2006/000230 WO2007030020A1 (en) 2005-09-05 2006-09-05 System for spore detection

Publications (3)

Publication Number Publication Date
GB0805032D0 GB0805032D0 (en) 2008-04-30
GB2445488A true GB2445488A (en) 2008-07-09
GB2445488B GB2445488B (en) 2010-10-06

Family

ID=37836073

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0805032A Expired - Fee Related GB2445488B (en) 2005-09-05 2006-09-05 System for spore detection

Country Status (4)

Country Link
AU (1) AU2006288014B2 (en)
GB (1) GB2445488B (en)
NZ (1) NZ542230A (en)
WO (1) WO2007030020A1 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120164681A1 (en) * 2006-09-05 2012-06-28 Lou Reinisch Method for Detection or Identification of Bacteria or Bacterial Spores
WO2008147227A1 (en) * 2007-05-31 2008-12-04 Veritide Limited Method for spore detection
US8512975B2 (en) 2008-07-24 2013-08-20 Biomerieux, Inc. Method for detection and characterization of a microorganism in a sample using time dependent spectroscopic measurements
US10167494B2 (en) 2008-10-31 2019-01-01 Biomerieux, Inc. Method for detection, characterization and/or identification of microorganisms in a sealed container
CA2740836C (en) 2008-10-31 2017-03-21 Biomerieux, Inc. Methods for separation, characterization and/or identification of microorganisms using spectroscopy
CN102272601B (en) 2008-10-31 2014-09-17 生物梅里埃公司 Methods for separation and characterization of microorganisms using identifier agents
EP2364447B1 (en) 2008-10-31 2019-06-12 Biomerieux, Inc Method for the identification of microorganisms
US8647835B2 (en) 2008-10-31 2014-02-11 BIO MéRIEUX, INC. Methods for separation, characterization and/or identification of microorganisms using spectroscopy
BRPI0919896A2 (en) 2008-10-31 2016-02-16 Bio Merieux Inc methods for the separation, characterization and / or identification of microorganisms by mass spectroscopy.
MX2011005988A (en) 2008-12-16 2011-09-01 Bio Merieux Inc Methods for the characterization of microorganisms on solid or semi-solid media.
FI20086237A0 (en) * 2008-12-23 2008-12-23 Marko Pudas Method and apparatus for detecting microorganisms in a liquid
US10316347B2 (en) 2014-06-26 2019-06-11 Ecolab Usa Inc. Endospore detection using hydrophobic collection material
CN115963074B (en) * 2023-02-23 2023-06-02 中国人民解放军国防科技大学 Method and system for rapidly detecting spore hypha ratio of microbial material

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002029388A2 (en) * 2000-10-04 2002-04-11 Genvec, Inc. Fluorescence detection
US6498041B1 (en) * 2000-08-18 2002-12-24 Echo Technologies, Inc. Optical sensors for rapid, sensitive detection and quantitation of bacterial spores
US6617591B1 (en) * 2001-12-03 2003-09-09 Sandia Corporation Method for remote detection of trace contaminants
WO2003089661A1 (en) * 2002-04-16 2003-10-30 The Johns Hopkins University Method and apparatus for detection of bioaerosols
WO2004015421A1 (en) * 2002-08-07 2004-02-19 Cyflo Oy Method and device for identifying micro organisms
WO2004048947A1 (en) * 2002-11-21 2004-06-10 Cdex, Inc. Methods and appartus for molecular species detection, inspection and classification using ultraviolet fluorescence

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003071269A2 (en) * 2002-02-20 2003-08-28 Affinium Pharmaceuticals, Inc. Methods and apparatuses for characterizing stability of biological molecules
US7587236B2 (en) * 2004-01-08 2009-09-08 Lawrence Livermore National Security, Llc Optical spectroscopy for the detection of ischemic tissue injury

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6498041B1 (en) * 2000-08-18 2002-12-24 Echo Technologies, Inc. Optical sensors for rapid, sensitive detection and quantitation of bacterial spores
WO2002029388A2 (en) * 2000-10-04 2002-04-11 Genvec, Inc. Fluorescence detection
US6617591B1 (en) * 2001-12-03 2003-09-09 Sandia Corporation Method for remote detection of trace contaminants
WO2003089661A1 (en) * 2002-04-16 2003-10-30 The Johns Hopkins University Method and apparatus for detection of bioaerosols
WO2004015421A1 (en) * 2002-08-07 2004-02-19 Cyflo Oy Method and device for identifying micro organisms
WO2004048947A1 (en) * 2002-11-21 2004-06-10 Cdex, Inc. Methods and appartus for molecular species detection, inspection and classification using ultraviolet fluorescence

Also Published As

Publication number Publication date
AU2006288014A1 (en) 2007-03-15
GB2445488B (en) 2010-10-06
WO2007030020A1 (en) 2007-03-15
GB0805032D0 (en) 2008-04-30
AU2006288014B2 (en) 2012-12-06
NZ542230A (en) 2008-05-30

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20200905