GB2444101A - Methods and compositions for detecting polynucleotides - Google Patents
Methods and compositions for detecting polynucleotidesInfo
- Publication number
- GB2444101A GB2444101A GB0608059A GB0608059A GB2444101A GB 2444101 A GB2444101 A GB 2444101A GB 0608059 A GB0608059 A GB 0608059A GB 0608059 A GB0608059 A GB 0608059A GB 2444101 A GB2444101 A GB 2444101A
- Authority
- GB
- United Kingdom
- Prior art keywords
- change
- optical property
- target polynucleotide
- rate
- dye
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 108091033319 polynucleotide Proteins 0.000 title abstract 7
- 102000040430 polynucleotide Human genes 0.000 title abstract 7
- 239000002157 polynucleotide Substances 0.000 title abstract 7
- 238000000034 method Methods 0.000 title abstract 2
- 239000000203 mixture Substances 0.000 title 1
- 230000003287 optical effect Effects 0.000 abstract 5
- 150000007523 nucleic acids Chemical class 0.000 abstract 4
- 239000011541 reaction mixture Substances 0.000 abstract 4
- 108020004707 nucleic acids Proteins 0.000 abstract 3
- 102000039446 nucleic acids Human genes 0.000 abstract 3
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 230000002596 correlated effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Methods of determining the presence or amount of a target polynucleotide in a sample are provided. A sample that contains a target polynucleotide, a nucleic acid analog that is complementary to a target nucleic acid sequence of the target polynucleotide, and a dye for which the rate of change in an optical property is different in the presence and absence of a target polynucleotide/nucleic acid analog hybrid are combined to produce a reaction mixture. The rate of change in an optical property of the dye in the reaction mixture is compared to a reference value characteristic of the rate of change in the optical property of the dye in a similar reaction mixture containing a known amount of a polynucleotide/nucleic acid analog hybrid to determine a relative rate of change in the optical property. The relative rate of change in the optical property of dye in the reaction mixture is correlated with the presence or amount of the specified target polynucleotide in the sample.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/US2006/006455 WO2008041960A2 (en) | 2006-02-24 | 2006-02-24 | Methods and compositions for detecting polynucleotides |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| GB0608059D0 GB0608059D0 (en) | 2006-05-31 |
| GB2444101A true GB2444101A (en) | 2008-05-28 |
Family
ID=36581138
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB0608059A Withdrawn GB2444101A (en) | 2006-02-24 | 2006-02-24 | Methods and compositions for detecting polynucleotides |
Country Status (2)
| Country | Link |
|---|---|
| GB (1) | GB2444101A (en) |
| WO (1) | WO2008041960A2 (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2694526A4 (en) * | 2011-04-01 | 2015-04-22 | Advandx Inc | Molecular gram stain |
| WO2014127214A1 (en) | 2013-02-15 | 2014-08-21 | Kala Pharmaceuticals, Inc. | Therapeutic compounds and uses thereof |
| US9688688B2 (en) | 2013-02-20 | 2017-06-27 | Kala Pharmaceuticals, Inc. | Crystalline forms of 4-((4-((4-fluoro-2-methyl-1H-indol-5-yl)oxy)-6-methoxyquinazolin-7-yl)oxy)-1-(2-oxa-7-azaspiro[3.5]nonan-7-yl)butan-1-one and uses thereof |
| ES2831625T3 (en) | 2013-02-20 | 2021-06-09 | Kala Pharmaceuticals Inc | Therapeutic compounds and their uses |
| US9890173B2 (en) | 2013-11-01 | 2018-02-13 | Kala Pharmaceuticals, Inc. | Crystalline forms of therapeutic compounds and uses thereof |
| WO2015066482A1 (en) | 2013-11-01 | 2015-05-07 | Kala Pharmaceuticals, Inc. | Crystalline forms of therapeutic compounds and uses thereof |
| AU2017324716B2 (en) | 2016-09-08 | 2020-08-13 | KALA BIO, Inc. | Crystalline forms of therapeutic compounds and uses thereof |
| AU2017324251A1 (en) | 2016-09-08 | 2019-03-21 | Kala Pharmaceuticals, Inc. | Crystalline forms of therapeutic compounds and uses thereof |
| AU2017324713B2 (en) | 2016-09-08 | 2020-08-13 | KALA BIO, Inc. | Crystalline forms of therapeutic compounds and uses thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2528843A1 (en) * | 2003-05-20 | 2005-02-24 | Investigen, Inc. | System for detecting polynucleotides |
-
2006
- 2006-02-24 GB GB0608059A patent/GB2444101A/en not_active Withdrawn
- 2006-02-24 WO PCT/US2006/006455 patent/WO2008041960A2/en active Application Filing
Non-Patent Citations (1)
| Title |
|---|
| Not yet advised * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2008041960A2 (en) | 2008-04-10 |
| WO2008041960A3 (en) | 2009-05-22 |
| GB0608059D0 (en) | 2006-05-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| WAP | Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1) |