GB2376024A - The production method of transgenic porcine producing human eryhropoietin and the transgenic porcine - Google Patents
The production method of transgenic porcine producing human eryhropoietin and the transgenic porcine Download PDFInfo
- Publication number
- GB2376024A GB2376024A GB0220971A GB0220971A GB2376024A GB 2376024 A GB2376024 A GB 2376024A GB 0220971 A GB0220971 A GB 0220971A GB 0220971 A GB0220971 A GB 0220971A GB 2376024 A GB2376024 A GB 2376024A
- Authority
- GB
- United Kingdom
- Prior art keywords
- porcine
- human
- transgenic
- epo
- transgenic porcine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000009261 transgenic effect Effects 0.000 title abstract description 6
- 238000004519 manufacturing process Methods 0.000 title 1
- 101000987586 Homo sapiens Eosinophil peroxidase Proteins 0.000 abstract description 5
- 102000044890 human EPO Human genes 0.000 abstract description 5
- 102000003951 Erythropoietin Human genes 0.000 abstract description 4
- 108090000394 Erythropoietin Proteins 0.000 abstract description 4
- 229940105423 erythropoietin Drugs 0.000 abstract description 4
- 239000013604 expression vector Substances 0.000 abstract description 4
- 239000012634 fragment Substances 0.000 abstract description 4
- OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 abstract description 4
- 230000024245 cell differentiation Effects 0.000 abstract description 2
- 239000003814 drug Substances 0.000 abstract description 2
- 235000013601 eggs Nutrition 0.000 abstract description 2
- 230000001456 gonadotroph Effects 0.000 abstract description 2
- 229940088597 hormone Drugs 0.000 abstract description 2
- 239000005556 hormone Substances 0.000 abstract description 2
- 238000010255 intramuscular injection Methods 0.000 abstract description 2
- 239000007927 intramuscular injection Substances 0.000 abstract description 2
- 210000005075 mammary gland Anatomy 0.000 abstract description 2
- 230000013011 mating Effects 0.000 abstract description 2
- 239000008267 milk Substances 0.000 abstract description 2
- 235000013336 milk Nutrition 0.000 abstract description 2
- 210000004080 milk Anatomy 0.000 abstract description 2
- 230000003248 secreting effect Effects 0.000 abstract description 2
- 101000920686 Homo sapiens Erythropoietin Proteins 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
- A01K67/0278—Knock-in vertebrates, e.g. humanised vertebrates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B25—HAND TOOLS; PORTABLE POWER-DRIVEN TOOLS; MANIPULATORS
- B25B—TOOLS OR BENCH DEVICES NOT OTHERWISE PROVIDED FOR, FOR FASTENING, CONNECTING, DISENGAGING OR HOLDING
- B25B21/00—Portable power-driven screw or nut setting or loosening tools; Attachments for drilling apparatus serving the same purpose
- B25B21/02—Portable power-driven screw or nut setting or loosening tools; Attachments for drilling apparatus serving the same purpose with means for imparting impact to screwdriver blade or nut socket
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B60—VEHICLES IN GENERAL
- B60B—VEHICLE WHEELS; CASTORS; AXLES FOR WHEELS OR CASTORS; INCREASING WHEEL ADHESION
- B60B29/00—Apparatus or tools for mounting or dismounting wheels
- B60B29/003—Wrenches, e.g. of the ratchet type
- B60B29/006—Wrenches, e.g. of the ratchet type with electric or pneumatic drive
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2207/00—Modified animals
- A01K2207/15—Humanized animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/108—Swine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/01—Animal expressing industrially exogenous proteins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Mechanical Engineering (AREA)
- Organic Chemistry (AREA)
- Environmental Sciences (AREA)
- Veterinary Medicine (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Animal Behavior & Ethology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Husbandry (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Disclosed are transgenic porcine capable of secreting human erythropoietin (EPO) in their milk and the preparation thereof. For the preparation of the transgenic porcine, a 2.6 kb WAP promoter from the mammary gland of a rat is first amplified by PCR. Along with this PCR product, the human EPO genome DNA fragment and an SV40 poly A DNA fragment are used to construct an expression vector. Separately, PMSG and human chorionic gonadotrophic (hCG) hormone are administered into porcine by intramuscular injection to induce porcine to ovulate excessively and the porcine were led to natural mating. From the porcine, the fertilized eggs in the first cell differentiation period are collected. Next, the expression vector is injected into male pronuclei which are immediately transplanted in surrogate mother porcine. The surrogate mother porcine are allowed to give birth to litters. Therefore, the present invention can produce the expensive medicine human EPO at low costs on a large scale, giving a contribution to the improving of human health.
Description
(57) Disclosed are transgenic porcine capable of secreting human
erythropoietin (EPO) in their milk and the preparation thereof. For the preparation of the transgenic porcine, a 2 6 kb WAP promoter from the mammary gland of a rat is first amplified by PCR. Along with this PCR product, the human EPO genome DNA fragment and an SV40 poly A DNA fragment are used to construct an expression vector. Separately, PMSG and human chorionic gonadotrophic (hCG} hormone are administered into porcine by intramuscular injection to induce porcine to ovulate excessively and the porcine were led to natural mating. From the porcine, the fertilized eggs in the first cell differentiation period are collected. Next, the expression vector is injected into male pronuclei which are immediately transplanted in surrogate mother porcine. The surrogate mother porcine are allowed to give birth to litters. Therefore, the present invention can produce the expensive medicine human EPO at low costs on a large scale, giving a contribution to the improving of human health.
P' - ral on ot lluman Ccnom c EPO DNA
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020000006888A KR100358754B1 (en) | 2000-02-14 | 2000-02-14 | The production method of transgenic porcine producing human erythropoietin and the transgenic porcine |
| PCT/KR2000/000675 WO2001059074A1 (en) | 2000-02-14 | 2000-06-28 | The production method of transgenic porcine producing human erythropoietin and the transgenic porcine |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| GB0220971D0 GB0220971D0 (en) | 2002-10-23 |
| GB2376024A true GB2376024A (en) | 2002-12-04 |
| GB2376024B GB2376024B (en) | 2004-09-22 |
Family
ID=19646330
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB0220971A Expired - Fee Related GB2376024B (en) | 2000-02-14 | 2000-06-28 | The production method of transgenic porcine producing human eryhropoietin and the transgenic porcine |
Country Status (4)
| Country | Link |
|---|---|
| KR (1) | KR100358754B1 (en) |
| AU (1) | AU2000255759A1 (en) |
| GB (1) | GB2376024B (en) |
| WO (1) | WO2001059074A1 (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100453571B1 (en) * | 2001-07-24 | 2004-10-20 | 대한민국 | Transgenic porcine capable of producing human erythropoietin and preparation method thereof |
| CN1283785C (en) * | 2002-05-20 | 2006-11-08 | 上海杰隆生物工程股份有限公司 | Method of producing human forcing erythrogenin using transgene animal mammary gland |
| GB0322196D0 (en) * | 2003-09-23 | 2003-10-22 | Cxr Biosciences Ltd | Excretable reporter systems |
| WO2005077404A1 (en) | 2004-02-13 | 2005-08-25 | Stem Cell Therapeutics Corp. | Use of luteinizing hormone (lh) and chorionic gonadotropin (hcg) for proliferation of neural stem cells and neurogenesis |
| WO2006079155A1 (en) * | 2005-01-25 | 2006-08-03 | Apollo Life Sciences Limited | Molecules and chimeric molecules thereof |
| KR100769291B1 (en) * | 2006-02-13 | 2007-10-24 | 조아제약주식회사 | Mammary gland-specific human erythropoietin expression vector transgenic animal and method for producing human erythropoietin using same |
| AU2007229301B2 (en) * | 2006-03-17 | 2013-08-01 | Stem Cell Therapeutics Corp. | Continuous dosing regimens for neural stem cell proliferating agents and neural stem cell differentiating agents |
| KR100935335B1 (en) * | 2007-09-12 | 2010-01-07 | 차의과학대학교 산학협력단 | Process for preparing hematopoietic cells in vivo using immunosuppressed erythropoietin-produceable transgenic porcines and animal bioreactor for the expansion of hematopoietic cells |
| AU2009266603B2 (en) | 2008-06-30 | 2013-01-24 | Cho-A Pharm Co., Ltd. | Gene of porcine alpha-s1 casein, a promoter of the same and use thereof |
| WO2010002082A1 (en) | 2008-06-30 | 2010-01-07 | Cho-A Pharm Co., Ltd. | A gene of porcine beta-casein, a promoter of the same and the use thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5955422A (en) * | 1983-12-13 | 1999-09-21 | Kirin-Amgen, Inc. | Production of erthropoietin |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5831141A (en) * | 1991-01-11 | 1998-11-03 | United States Of America As Represented By The Department Of Health And Human Services | Expression of a heterologous polypeptide in mammary tissue of transgenic non-human mammals using a long whey acidic protein promoter |
| US5959171A (en) * | 1994-08-17 | 1999-09-28 | Pharming B.V. | Method for the production of biologically active polypeptides in a mammal's |
-
2000
- 2000-02-14 KR KR1020000006888A patent/KR100358754B1/en active IP Right Grant
- 2000-06-28 WO PCT/KR2000/000675 patent/WO2001059074A1/en active Search and Examination
- 2000-06-28 GB GB0220971A patent/GB2376024B/en not_active Expired - Fee Related
- 2000-06-28 AU AU2000255759A patent/AU2000255759A1/en not_active Abandoned
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5955422A (en) * | 1983-12-13 | 1999-09-21 | Kirin-Amgen, Inc. | Production of erthropoietin |
Non-Patent Citations (8)
| Title |
|---|
| Biol. Res., Vol.28, 1995, pp.141-153 * |
| Blood, Vol.85, 1995, pp.2735-2741 * |
| DNA Cell Biol., Vol.18, 1999, p.845 * |
| Eur. J. Biochem., Vol.245, 1997, pp.482-489 * |
| Mol. Biol. Res., Vol.5, 1989, pp.255-261 * |
| P.N.A.S., Vol.87, 1990, pp.5178-5182 * |
| Transgenic Res., Vol.6, 1997, pp.75-84 * |
| Transgenic Res., Vol.7, 1998, pp.311-317 * |
Also Published As
| Publication number | Publication date |
|---|---|
| GB2376024B (en) | 2004-09-22 |
| KR20010081456A (en) | 2001-08-29 |
| KR100358754B1 (en) | 2002-11-07 |
| WO2001059074A1 (en) | 2001-08-16 |
| GB0220971D0 (en) | 2002-10-23 |
| AU2000255759A1 (en) | 2001-08-20 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PCNP | Patent ceased through non-payment of renewal fee |
Effective date: 20180628 |