GB2368641A - Methods and compositions fo assaying analytes - Google Patents

Methods and compositions fo assaying analytes

Info

Publication number
GB2368641A
GB2368641A GB0200425A GB0200425A GB2368641A GB 2368641 A GB2368641 A GB 2368641A GB 0200425 A GB0200425 A GB 0200425A GB 0200425 A GB0200425 A GB 0200425A GB 2368641 A GB2368641 A GB 2368641A
Authority
GB
United Kingdom
Prior art keywords
binding affinity
analytes
modified enzymes
compositions
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB0200425A
Other versions
GB0200425D0 (en
GB2368641B (en
Inventor
Chong-Sheng Yuan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
General Atomics Corp
Original Assignee
General Atomics Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/347,878 external-priority patent/US6376210B1/en
Application filed by General Atomics Corp filed Critical General Atomics Corp
Publication of GB0200425D0 publication Critical patent/GB0200425D0/en
Publication of GB2368641A publication Critical patent/GB2368641A/en
Application granted granted Critical
Publication of GB2368641B publication Critical patent/GB2368641B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Inorganic Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Compositions and methods for assaying analytes, preferably, small molecule analytes are provided. Assay methods employ, in place of antibodies or molecules that bind to target analytes or substrates, modified enzymes, called substrate trapping enzymes. These modified enzymes retain binding affinity or have enhanced binding affinity for a target substrate or analyte, but have attenuated catalytic activity with respect to that substrate or analyte. The modified enzymes are provided. In particular, mutant S-adenosylhomocysteine (SAH) hydrolases, substantially retaining binding affinity or having enhanced binding affinity for homocysteine or S-adenosylhomocysteine but having attenuated catalytic activity, are provided. Conjugates of the modified enzymes and a facilitating agent, such as agents that aid in purification or linkage to a solid support are also provided.
GB0200425A 1999-07-06 2000-06-30 Methods and compositions for assaying analytes Expired - Fee Related GB2368641B (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/347,878 US6376210B1 (en) 1999-07-06 1999-07-06 Methods and compositions for assaying analytes
US45720599A 1999-12-06 1999-12-06
PCT/US2000/018057 WO2001002600A2 (en) 1999-07-06 2000-06-30 Detection of analytes using attenuated enzymes

Publications (3)

Publication Number Publication Date
GB0200425D0 GB0200425D0 (en) 2002-02-27
GB2368641A true GB2368641A (en) 2002-05-08
GB2368641B GB2368641B (en) 2004-10-06

Family

ID=26995460

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0200425A Expired - Fee Related GB2368641B (en) 1999-07-06 2000-06-30 Methods and compositions for assaying analytes

Country Status (4)

Country Link
AU (1) AU5781800A (en)
CA (1) CA2377665A1 (en)
GB (1) GB2368641B (en)
WO (1) WO2001002600A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7192729B2 (en) 1999-07-06 2007-03-20 General Atomics Methods for assaying homocysteine
JP4660094B2 (en) 2002-03-26 2011-03-30 ゼンサン （シャンハイ） サイ−テク． リミテッド ErbB3-based methods and compositions for treating neoplasms
WO2003106971A2 (en) 2002-06-17 2003-12-24 The Regents Of The University Of Colorado HUMAN CYSTATHIONINE β-SYNTHASE VARIANTS AND METHODS OF PRODU CTION THEREOF
US7098189B2 (en) 2002-12-16 2006-08-29 Kimberly-Clark Worldwide, Inc. Wound and skin care compositions
JP2006512080A (en) * 2002-12-31 2006-04-13 ロディ ファーマ，インコーポレイティド Bindingzyme array and high-throughput proteomics method
US7097968B2 (en) * 2003-07-10 2006-08-29 General Atomics Methods and compositions for assaying homocysteine
US8476034B2 (en) 2003-07-10 2013-07-02 General Atomics Methods and compositions for assaying homocysteine
US7485666B2 (en) 2004-06-17 2009-02-03 Kimberly-Clark Worldwide, Inc. Vaginal health products
US9675678B2 (en) 2013-01-29 2017-06-13 The Regents Of The University Of Colorado, A Body Corporate Compositions and methods for treatment of homocystinuria
WO2015006934A1 (en) * 2013-07-17 2015-01-22 国家纳米科学中心 Small-molecule drug screening chip, construction method therefor and application thereof
CN104111337B (en) * 2014-05-07 2016-01-06 山东博科生物产业有限公司 The homocysteine detection kit of strong interference immunity
CN106434786B (en) * 2015-08-21 2020-08-25 光明乳业股份有限公司 Method for preparing exopolysaccharide by fermenting lactobacillus casei
SG10201912972SA (en) 2015-11-09 2020-03-30 Univ Colorado Regents Compositions and methods for treatment of homocystinuria
AU2018254410A1 (en) 2017-04-17 2019-10-10 The Regents Of The University Of Colorado, A Body Corporate Optimization of enzyme replacement therapy for treatment of homocystinuria
CN108486007B (en) * 2018-03-22 2020-10-13 嘉兴益诺康生物科技有限公司 Lactobacillus casei strain for reducing blood uric acid, probiotic composition and application thereof
CN110097928B (en) * 2019-04-17 2022-03-11 广东省科学院微生物研究所(广东省微生物分析检测中心) Prediction method and prediction model for predicting tissue trace element content based on intestinal flora
CN114460159B (en) * 2022-02-17 2023-11-03 河南中医药大学 ALP activity detection kit based on photo-ATRP signal amplification strategy and application method thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3881931T2 (en) * 1987-04-10 1993-12-16 Univ Southern Australia Determination of potassium ions in liquids.
GB9022304D0 (en) * 1990-10-15 1990-11-28 Ares Serono Res & Dev Ltd Assay technique
PT726322E (en) * 1992-01-22 2003-09-30 Axis Shield Asa ANALYSIS FOR DETERMINATION OF HOMOCYSTEIN
US5679548A (en) * 1993-02-02 1997-10-21 The Scripps Research Institute Methods for producing polypeptide metal binding sites and compositions thereof
JP2001508647A (en) * 1996-11-04 2001-07-03 メルク フロスト カナダ アンド カンパニー Hydrolase binding assay
DE19757571A1 (en) * 1997-12-23 1999-06-24 Hartmut Prof Dr Oswald Detection of adenosine in biological fluids, by forming enzyme complex

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Not yet advised *

Also Published As

Publication number Publication date
CA2377665A1 (en) 2001-01-11
WO2001002600A9 (en) 2002-07-25
WO2001002600A2 (en) 2001-01-11
GB0200425D0 (en) 2002-02-27
GB2368641B (en) 2004-10-06
WO2001002600A3 (en) 2002-01-10
AU5781800A (en) 2001-01-22

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20090630