GB2361237A - Selection system - Google Patents

Selection system

Info

Publication number
GB2361237A
GB2361237A GB0026143A GB0026143A GB2361237A GB 2361237 A GB2361237 A GB 2361237A GB 0026143 A GB0026143 A GB 0026143A GB 0026143 A GB0026143 A GB 0026143A GB 2361237 A GB2361237 A GB 2361237A
Authority
GB
United Kingdom
Prior art keywords
virus
polypeptide
fusion
selection system
selection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB0026143A
Other versions
GB0026143D0 (en
Inventor
Lutz Riechmann
Peter Kristensen
Jean-Luc Jestin
Gregory Paul Winter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DIVERSYS Ltd
Original Assignee
DIVERSYS Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9810223.9A external-priority patent/GB9810223D0/en
Priority claimed from GBGB9810228.8A external-priority patent/GB9810228D0/en
Application filed by DIVERSYS Ltd filed Critical DIVERSYS Ltd
Priority to GB0227323A priority Critical patent/GB2379933B/en
Publication of GB0026143D0 publication Critical patent/GB0026143D0/en
Publication of GB2361237A publication Critical patent/GB2361237A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/41—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a Myc-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/43—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a FLAG-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Control Of Indicators Other Than Cathode Ray Tubes (AREA)
  • Circuits Of Receivers In General (AREA)
  • Vehicle Body Suspensions (AREA)
  • Air Bags (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The present invention concerns a method for the selection of a virus comprising the steps of: (a) providing a virus encoding and displaying a fusion polypeptide, said fusion polypeptide comprising a heterologous polypeptide inserted into the sequence of a viral coat protein polypeptide, wherein said virus comprises a cleavable site located within a displayed polypeptide; (b) exposing the virus to a cleaving agent; (c) propagating the virus comprising intact fusion protein.
GB0026143A 1998-05-13 1999-05-13 Selection system Withdrawn GB2361237A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB0227323A GB2379933B (en) 1998-05-13 1999-05-13 Selection system for phagemids using proteolytically sensitive helper phage

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9810223.9A GB9810223D0 (en) 1998-05-13 1998-05-13 Selection system
GBGB9810228.8A GB9810228D0 (en) 1998-05-13 1998-05-13 Selection method
PCT/GB1999/001526 WO1999058655A2 (en) 1998-05-13 1999-05-13 Phage display selection system for folded proteins

Publications (2)

Publication Number Publication Date
GB0026143D0 GB0026143D0 (en) 2000-12-13
GB2361237A true GB2361237A (en) 2001-10-17

Family

ID=26313668

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0026143A Withdrawn GB2361237A (en) 1998-05-13 1999-05-13 Selection system

Country Status (14)

Country Link
US (2) US7442159B1 (en)
EP (2) EP1950295A1 (en)
JP (2) JP4493846B2 (en)
AT (1) ATE380865T1 (en)
AU (1) AU762814B2 (en)
CA (1) CA2328422A1 (en)
CY (1) CY1107293T1 (en)
DE (1) DE69937735T2 (en)
DK (1) DK1078051T3 (en)
ES (1) ES2299244T3 (en)
GB (1) GB2361237A (en)
NO (1) NO329172B1 (en)
PT (1) PT1078051E (en)
WO (1) WO1999058655A2 (en)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000006717A2 (en) 1998-07-27 2000-02-10 Genentech, Inc. Improved transformation efficiency in phage display through modification of a coat protein
PT1144607E (en) 1999-07-20 2009-04-22 Morphosys Ag Methods for displaying (poly)peptides/proteins on bacteriophage particles via disulfide bonds
JP2003523742A (en) * 2000-02-03 2003-08-12 ドマンティス リミテッド Combinatorial protein domains
GB0019049D0 (en) * 2000-08-04 2000-09-27 Neutec Pharma Plc Combinatorial chemistry method
AU2001277614A1 (en) * 2000-08-07 2002-02-18 Domantis Limited Novel proteins
DE10135039C1 (en) * 2001-07-18 2003-03-13 Nemod Immuntherapie Ag Method for isolating large variances of specific molecules for a target molecule from phagemid gene libraries
AU2004239065B2 (en) 2003-05-14 2008-05-15 Domantis Limited A process for recovering polypeptides that unfold reversibly from a polypeptide repertoire
EP1902131B1 (en) 2005-07-08 2009-11-11 University of Zurich Phage display using cotranslational translocation of fusion polypeptides
GB2428293A (en) * 2005-07-13 2007-01-24 Domantis Ltd Phage display libraries
ATE524579T1 (en) * 2005-07-21 2011-09-15 Aebi Ltd METHODS AND COMPOSITIONS FOR IDENTIFYING A PEPTIDE HAVING INTERMOLECULAR INTERACTION WITH A TARGET OF INTEREST
GB0515115D0 (en) 2005-07-22 2005-08-31 Isogenica Ltd Peptide characterisation
AU2007262635B2 (en) * 2006-06-23 2014-09-11 Adc Therapeutics Sa Polynucleotides and polypeptide sequences involved in cancer
CA2683791A1 (en) 2007-06-06 2008-12-11 Domantis Limited Polypeptides, antibody variable domains & antagonists
AU2008290539B2 (en) 2007-08-21 2013-07-25 Morphosys Ag Improved methods for the formation of disulphide bonds
AR069495A1 (en) 2007-11-30 2010-01-27 Glaxo Group Ltd CONSTRUCTION OF ANTIGEN UNION FOR THE TREATMENT OF CANCER OR INFLAMMATORY DISEASES (ASTHMA, ARTHRITIS OR ARTROSIS)
CN104829714A (en) 2008-11-03 2015-08-12 阿莱斯亚生物疗法股份有限公司 Antibodies that specifically block the biological activity of a tumor antigen
WO2010063818A2 (en) 2008-12-05 2010-06-10 Glaxo Group Limited Methods for selecting protease resistant polypeptides
WO2010097385A1 (en) 2009-02-24 2010-09-02 Glaxo Group Limited Antigen-binding constructs
CA2753332A1 (en) 2009-02-24 2010-09-02 Glaxo Group Limited Antigen-binding constructs
CA2753263A1 (en) 2009-02-24 2010-09-02 Glaxo Group Limited Multivalent and/or multispecific rankl-binding constructs
US20130236467A1 (en) 2010-11-24 2013-09-12 Jeremy Griggs Multispecific antigen binding proteins targeting hgf
SMT201900505T1 (en) 2011-03-31 2019-11-13 Adc Therapeutics Sa Antibodies against kidney associated antigen 1 and antigen binding fragments thereof
CN103842380A (en) 2011-07-27 2014-06-04 葛兰素集团有限公司 Anti-VEGF single variable domains fused to Fc domains
AU2012327806B2 (en) 2011-10-27 2018-07-19 The Governors Of The University Of Alberta Device and method for bacterial culture and assay
PT2802351T (en) 2012-01-09 2019-06-27 Adc Therapeutics Sa Agents for treating triple negative breast cancer
KR20250051130A (en) * 2013-09-25 2025-04-16 싸이톰스 테라퓨틱스, 인크. Matrix metalloproteinase substrates and other cleavable moieties and methods of use thereof
BR112016017649B1 (en) 2014-01-31 2024-01-30 Cytomx Therapeutics, Inc ISOLATED POLYPEPTIDE COMPRISING MATRIPTASE AND PLASMINOGEN ACTIVATOR U SUBSTRATES AND OTHER CLEAABLE MOTIONS, PHARMACEUTICAL COMPOSITION COMPRISING SAID POLYPEPTIDE, AS WELL AS METHODS FOR PRODUCING AND MANUFACTURING AN ISOLATED POLYPEPTIDE COMPRISING A CLEAABLE MOTION AND USE OF THE PHARMACEUTICAL COMPOSITION AND THERAPEUTICLY EFFECTIVE AMOUNT OF SAID COMPOSITION IN THE TREATMENT OF A DISORDER OR ILLNESS
US20180340174A1 (en) * 2014-11-11 2018-11-29 Nanocore Aps Method for identification of molecules with desired characteristics
MA41374A (en) 2015-01-20 2017-11-28 Cytomx Therapeutics Inc MATRIX METALLOPROTEASE CLIVABLE AND SERINE PROTEASE CLIVABLE SUBSTRATES AND METHODS OF USE THEREOF
SG11201706882VA (en) 2015-03-31 2017-09-28 Vhsquared Ltd Polypeptides
CA2981098A1 (en) * 2015-03-31 2016-10-06 Vhsquared Limited Peptide construct having a protease-cleavable linker
EP3827116A4 (en) 2018-07-23 2022-05-11 The Governors of the University of Alberta GENETICALLY ENCODED BICYCLIC PEPTIDE LIBRARIES
AU2019394972A1 (en) 2018-12-06 2021-06-03 Cytomx Therapeutics, Inc. Matrix metalloprotease-cleavable and serine or cysteine protease-cleavable substrates and methods of use thereof
CA3144567A1 (en) 2019-06-21 2020-12-24 Scott Crowe Polypeptides
JP2022537268A (en) * 2019-06-21 2022-08-25 ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング A novel mechanism that regulates RNA virus replication and gene expression

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992001047A1 (en) * 1990-07-10 1992-01-23 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs

Family Cites Families (8)

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US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
JP2919890B2 (en) 1988-11-11 1999-07-19 メディカル リサーチ カウンスル Single domain ligand, receptor consisting of the ligand, method for producing the same, and use of the ligand and the receptor
CA2016842A1 (en) 1989-05-16 1990-11-16 Richard A. Lerner Method for tapping the immunological repertoire
US5723286A (en) * 1990-06-20 1998-03-03 Affymax Technologies N.V. Peptide library and screening systems
JPH06508511A (en) * 1990-07-10 1994-09-29 ケンブリッジ　アンティボディー　テクノロジー　リミティド Method for producing specific binding pair members
JP4146512B2 (en) * 1991-03-01 2008-09-10 ダイアックス コープ． Small protein
DE4122599C2 (en) * 1991-07-08 1993-11-11 Deutsches Krebsforsch Phagemid for screening antibodies
DE69233745D1 (en) 1991-12-02 2008-10-23 Cambridge Antibody Tech Preparation of Autoantibodies on Phage Surfaces Starting from Antibody Segment Libraries

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992001047A1 (en) * 1990-07-10 1992-01-23 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Folding & Design; 3 (5), pp 321-328 (1998). Kristensen & Winter *
J. Immunol. Methods; 189 (1), pp 73-82 (1996). Ward et al *
Mol Divers; 1 (3), pp 165-176 (1996). McConnel et al *
TibTech; 12 (1), pp 173-184 (1994) . Clackson & Wells *

Also Published As

Publication number Publication date
EP1078051A2 (en) 2001-02-28
JP5161865B2 (en) 2013-03-13
AU3941399A (en) 1999-11-29
EP1078051B1 (en) 2007-12-12
ATE380865T1 (en) 2007-12-15
WO1999058655A3 (en) 2000-01-27
US7442159B1 (en) 2008-10-28
ES2299244T3 (en) 2008-05-16
NO329172B1 (en) 2010-09-06
JP4493846B2 (en) 2010-06-30
PT1078051E (en) 2008-03-17
DE69937735D1 (en) 2008-01-24
JP2002514413A (en) 2002-05-21
AU762814B2 (en) 2003-07-03
GB0026143D0 (en) 2000-12-13
WO1999058655A2 (en) 1999-11-18
CY1107293T1 (en) 2012-11-21
US8680018B2 (en) 2014-03-25
DE69937735T2 (en) 2008-11-27
JP2010148507A (en) 2010-07-08
US20090203542A1 (en) 2009-08-13
NO20005628D0 (en) 2000-11-08
NO20005628L (en) 2001-01-12
CA2328422A1 (en) 1999-11-18
DK1078051T3 (en) 2008-04-07
EP1950295A1 (en) 2008-07-30

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Legal Events

Date Code Title Description
WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)