GB2379933B - Selection system for phagemids using proteolytically sensitive helper phage - Google Patents

Selection system for phagemids using proteolytically sensitive helper phage

Info

Publication number
GB2379933B
GB2379933B GB0227323A GB0227323A GB2379933B GB 2379933 B GB2379933 B GB 2379933B GB 0227323 A GB0227323 A GB 0227323A GB 0227323 A GB0227323 A GB 0227323A GB 2379933 B GB2379933 B GB 2379933B
Authority
GB
United Kingdom
Prior art keywords
phagemids
selection system
helper phage
proteolytically sensitive
sensitive helper
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
GB0227323A
Other versions
GB0227323D0 (en
GB2379933A (en
Inventor
Lutz Riechmann
Peter Kristensen
Jean-Luc Jestin
Gregory Paul Winter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Domantis Ltd
Original Assignee
Domantis Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9810228.8A external-priority patent/GB9810228D0/en
Priority claimed from GBGB9810223.9A external-priority patent/GB9810223D0/en
Application filed by Domantis Ltd filed Critical Domantis Ltd
Priority claimed from GB0026143A external-priority patent/GB2361237A/en
Publication of GB0227323D0 publication Critical patent/GB0227323D0/en
Publication of GB2379933A publication Critical patent/GB2379933A/en
Application granted granted Critical
Publication of GB2379933B publication Critical patent/GB2379933B/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/41—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a Myc-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/43—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a FLAG-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)
GB0227323A 1998-05-13 1999-05-13 Selection system for phagemids using proteolytically sensitive helper phage Expired - Lifetime GB2379933B (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9810228.8A GB9810228D0 (en) 1998-05-13 1998-05-13 Selection method
GBGB9810223.9A GB9810223D0 (en) 1998-05-13 1998-05-13 Selection system
GB0026143A GB2361237A (en) 1998-05-13 1999-05-13 Selection system

Publications (3)

Publication Number Publication Date
GB0227323D0 GB0227323D0 (en) 2002-12-31
GB2379933A GB2379933A (en) 2003-03-26
GB2379933B true GB2379933B (en) 2003-07-09

Family

ID=27255951

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0227323A Expired - Lifetime GB2379933B (en) 1998-05-13 1999-05-13 Selection system for phagemids using proteolytically sensitive helper phage

Country Status (1)

Country Link
GB (1) GB2379933B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2428293A (en) * 2005-07-13 2007-01-24 Domantis Ltd Phage display libraries

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992001047A1 (en) * 1990-07-10 1992-01-23 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992001047A1 (en) * 1990-07-10 1992-01-23 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
JOURNAL OF IMMUNOLOGICAL METHODS, 1996, VOL 189, WARD R.L. ET AL., PAGES 73-82. *
MOLECULAR DIVERSITY, 1995, VOL 1, MCCONNELL, S.J. ET AL., PAGES 165-176. *
NATURE, 1993, VOL 260, MATTHEWS, D.J. AND WELLS, J.A. PAGES 1113-1117. *

Also Published As

Publication number Publication date
GB0227323D0 (en) 2002-12-31
GB2379933A (en) 2003-03-26

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Legal Events

Date Code Title Description
PE20 Patent expired after termination of 20 years

Expiry date: 20190512