GB2303856A - Transgenic plants producing trehalose - Google Patents
Transgenic plants producing trehaloseInfo
- Publication number
- GB2303856A GB2303856A GB9627137A GB9627137A GB2303856A GB 2303856 A GB2303856 A GB 2303856A GB 9627137 A GB9627137 A GB 9627137A GB 9627137 A GB9627137 A GB 9627137A GB 2303856 A GB2303856 A GB 2303856A
- Authority
- GB
- United Kingdom
- Prior art keywords
- plants
- trehalose
- gene
- transgenic plants
- plants producing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
Abstract
The present invention concerns transgenic plants producing trehalose and methods of increasing the trehalose content of plants. According to the invention, the plants of interest are transformed with the coding sequence of a gene for trehalose-6-phosphate synthase fused to a non-constitutive plant promoter, which allows for temporal, topological or stress-induced control over the expression of the gene. The invention can be used for protecting staple crop plants against drought, high salinity or temperature extremes and for improving the storage properties of harvested plants including green food stuffs, picked fruits and ornamental plants.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FI943133A FI943133A0 (en) | 1994-06-29 | 1994-06-29 | Transgene vaexter |
PCT/FI1995/000377 WO1996000789A1 (en) | 1994-06-29 | 1995-06-29 | Transgenic plants producing trehalose |
Publications (3)
Publication Number | Publication Date |
---|---|
GB9627137D0 GB9627137D0 (en) | 1997-02-19 |
GB2303856A true GB2303856A (en) | 1997-03-05 |
GB2303856B GB2303856B (en) | 1998-12-30 |
Family
ID=8541026
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB9627137A Expired - Fee Related GB2303856B (en) | 1994-06-29 | 1995-06-29 | Transgenic plants producing trehalose |
Country Status (11)
Country | Link |
---|---|
EP (1) | EP0763118A1 (en) |
JP (1) | JPH10501978A (en) |
CN (1) | CN1159833A (en) |
AU (1) | AU699391B2 (en) |
CA (1) | CA2193861A1 (en) |
CZ (1) | CZ294329B6 (en) |
FI (1) | FI943133A0 (en) |
GB (1) | GB2303856B (en) |
HU (1) | HU221613B (en) |
NZ (1) | NZ288635A (en) |
WO (1) | WO1996000789A1 (en) |
Families Citing this family (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6118047A (en) | 1993-08-25 | 2000-09-12 | Dekalb Genetic Corporation | Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction |
US5780709A (en) * | 1993-08-25 | 1998-07-14 | Dekalb Genetics Corporation | Transgenic maize with increased mannitol content |
IL116564A0 (en) * | 1995-01-04 | 1996-03-31 | Mogen Int | Process for producing trehalose in plants |
EP0784095A3 (en) | 1996-01-12 | 1997-12-29 | Mogen International N.V. | Enhanced accummulation of trehalose in plants |
IN1997CH00924A (en) | 1996-05-03 | 2005-03-04 | Syngenta Mogen Bv | Regulating metabolism by modifying the level of trehalose-6-phosphate |
MX205414B (en) * | 1996-05-08 | 2001-12-07 | Univ Mexico Nacional Autonoma | METHOD OF INCREASING TREHALOSE IN ORGANISMS BY TRANSFORMATION OF SELAGINELLA LEPIDOPHYLIA TREHALOSE-6-PHOSPHATE SINTASE/PHOSPHATASE cDNA. |
ES2110918B1 (en) * | 1996-07-04 | 1998-08-01 | Univ Poltecnica De Valencia | OBTAINING TOLERANT PLANTS TO OSMOTIC STRESS THROUGH GENETIC MANIPULATION OF CARBOHYDRATE METABOLISM. |
CN1112444C (en) * | 1996-10-24 | 2003-06-25 | 日本烟草产业株式会社 | Method for controlling water content of plant |
GB9703681D0 (en) * | 1997-02-21 | 1997-04-09 | Gene Shears Pty Ltd | Protein complemention |
TW466116B (en) * | 1997-03-04 | 2001-12-01 | Hayashibara Biochem Lab | Reduction inhibitory agent for active-oxygen eliminating activity, method for inhibiting the reduction of said activity, and composition containing said agent |
KR20010012147A (en) * | 1997-05-02 | 2001-02-15 | 레오 씨어드 멜커스 | Regulating metabolism by modifying the level of trehalose-6-phosphate by inhibiting endogenous trehalase levels |
ZA989886B (en) | 1997-10-30 | 1999-04-30 | Mogen Int | Nuclear male sterile plants method of producing same and methods to restore fertility |
AU1559799A (en) * | 1997-10-30 | 1999-05-24 | Mogen International N.V. | Novel high-fermenting microorganisms |
ZA989782B (en) | 1997-10-30 | 1999-05-04 | Mogen Int | Pre-and postharvest inhibition of remobilisation of storage compounds |
AU2412499A (en) * | 1997-10-30 | 1999-05-31 | Mogen International N.V. | Novel high-fermenting microorganisms |
RO122151B1 (en) * | 1998-01-22 | 2009-01-30 | National Research Council Of Canada | Process for modifying concentration levels of secondary metabolic compounds in plants |
JP2002505875A (en) * | 1998-03-11 | 2002-02-26 | ノバルティス アクチエンゲゼルシャフト | Expression of trehalose biosynthesis gene in plants |
DE19832334A1 (en) * | 1998-07-17 | 2000-01-20 | Norika Nordring Kartoffelzucht | Use of a glucosylglycerol phosphate synthase gene for glucosylglycerol production and generation of transgenic plants with increased tolerance against environmental stress |
EP1002867A1 (en) | 1998-10-15 | 2000-05-24 | K.U. Leuven Research & Development | Specific genetic modification of the activity of trehalose-6-phosphate synthase and expression in a homologous or heterologous environment |
GB0100105D0 (en) * | 2001-01-04 | 2001-02-14 | Leuven K U Res & Dev | The use of plant TPS (Trehalose-6-phosphate syntase) as a selectable marker for plant transformation |
KR100440725B1 (en) | 2002-06-20 | 2004-07-15 | 주식회사 그린진 바이오텍 | A Method for Increasing an Abiotic-Resistance in Monocot Plants |
US20040187175A1 (en) * | 2002-11-06 | 2004-09-23 | Wu Ray J. | TPS plant gene constructs and transformants |
CN102286481B (en) * | 2004-04-20 | 2014-07-23 | 辛根塔参与股份公司 | Regulatory sequences for expressing gene products in plant reproductive tissue |
CN101586107A (en) * | 2009-07-08 | 2009-11-25 | 四川农业大学 | Gene sequence of trehalose-6-phosphate synthetase derived from selaginella pulvinata |
WO2012145592A1 (en) * | 2011-04-22 | 2012-10-26 | Danisco Us Inc. | Filamentous fungi having an altered viscosity phenotype |
CN113016524A (en) * | 2019-12-24 | 2021-06-25 | 东北农业大学 | Method for improving alkali resistance of rice |
CN112980849B (en) * | 2021-05-10 | 2022-10-18 | 长江师范学院 | Active siRNA for preventing and treating clubroot by targeting plasmodiophora pbTPS1 gene and application thereof |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0451896A1 (en) * | 1990-03-28 | 1991-10-16 | Gist-Brocades N.V. | New yeast strains with enhanced trehalose content, process to obtain such yeasts and the use of these yeasts |
WO1993017093A2 (en) * | 1992-02-14 | 1993-09-02 | Oy Alko Ab | Increasing the trehalose content of organisms by transforming them with combinations of the structural genes for trehalose synthase |
WO1995001446A1 (en) * | 1993-06-30 | 1995-01-12 | Mogen International N.V. | Production of trehalose in plants |
WO1995006126A1 (en) * | 1993-08-24 | 1995-03-02 | Mogen International N.V. | Production of trehalose in plants |
-
1994
- 1994-06-29 FI FI943133A patent/FI943133A0/en not_active Application Discontinuation
-
1995
- 1995-06-29 EP EP95923355A patent/EP0763118A1/en not_active Ceased
- 1995-06-29 WO PCT/FI1995/000377 patent/WO1996000789A1/en not_active Application Discontinuation
- 1995-06-29 JP JP8502849A patent/JPH10501978A/en not_active Ceased
- 1995-06-29 HU HU9603608V patent/HU221613B/en not_active IP Right Cessation
- 1995-06-29 CN CN95194807A patent/CN1159833A/en active Pending
- 1995-06-29 NZ NZ288635A patent/NZ288635A/en unknown
- 1995-06-29 AU AU27944/95A patent/AU699391B2/en not_active Ceased
- 1995-06-29 GB GB9627137A patent/GB2303856B/en not_active Expired - Fee Related
- 1995-06-29 CA CA002193861A patent/CA2193861A1/en not_active Abandoned
- 1995-06-29 CZ CZ19963782A patent/CZ294329B6/en not_active IP Right Cessation
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0451896A1 (en) * | 1990-03-28 | 1991-10-16 | Gist-Brocades N.V. | New yeast strains with enhanced trehalose content, process to obtain such yeasts and the use of these yeasts |
WO1993017093A2 (en) * | 1992-02-14 | 1993-09-02 | Oy Alko Ab | Increasing the trehalose content of organisms by transforming them with combinations of the structural genes for trehalose synthase |
WO1995001446A1 (en) * | 1993-06-30 | 1995-01-12 | Mogen International N.V. | Production of trehalose in plants |
WO1995006126A1 (en) * | 1993-08-24 | 1995-03-02 | Mogen International N.V. | Production of trehalose in plants |
Non-Patent Citations (2)
Title |
---|
Eur.J.Biochem. 1993,216(3),849-861 * |
FEMS Microbiology Letters 1992,94(3),271-276 * |
Also Published As
Publication number | Publication date |
---|---|
FI943133A0 (en) | 1994-06-29 |
CZ378296A3 (en) | 1998-03-18 |
CZ294329B6 (en) | 2004-12-15 |
GB9627137D0 (en) | 1997-02-19 |
NZ288635A (en) | 1998-12-23 |
AU2794495A (en) | 1996-01-25 |
EP0763118A1 (en) | 1997-03-19 |
HU221613B (en) | 2002-11-28 |
AU699391B2 (en) | 1998-12-03 |
CN1159833A (en) | 1997-09-17 |
HUT75659A (en) | 1997-05-28 |
WO1996000789A1 (en) | 1996-01-11 |
HUP9603608D0 (en) | 1997-02-28 |
CA2193861A1 (en) | 1996-01-11 |
JPH10501978A (en) | 1998-02-24 |
GB2303856B (en) | 1998-12-30 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
732E | Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977) | ||
PCNP | Patent ceased through non-payment of renewal fee |
Effective date: 20050629 |