GB2303856A - Transgenic plants producing trehalose - Google Patents

Transgenic plants producing trehalose

Info

Publication number
GB2303856A
GB2303856A GB9627137A GB9627137A GB2303856A GB 2303856 A GB2303856 A GB 2303856A GB 9627137 A GB9627137 A GB 9627137A GB 9627137 A GB9627137 A GB 9627137A GB 2303856 A GB2303856 A GB 2303856A
Authority
GB
United Kingdom
Prior art keywords
plants
trehalose
gene
transgenic plants
plants producing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB9627137A
Other versions
GB9627137D0 (en
GB2303856B (en
Inventor
John Londesborough
Outi Tunnela
Kjell-Ove Holmstrom
Einar Mantyla
Bjorn Welin
Abul Mandal
E Tapio Palva
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alko Oy AB
Original Assignee
Alko Oy AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alko Oy AB filed Critical Alko Oy AB
Publication of GB9627137D0 publication Critical patent/GB9627137D0/en
Publication of GB2303856A publication Critical patent/GB2303856A/en
Application granted granted Critical
Publication of GB2303856B publication Critical patent/GB2303856B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

Abstract

The present invention concerns transgenic plants producing trehalose and methods of increasing the trehalose content of plants. According to the invention, the plants of interest are transformed with the coding sequence of a gene for trehalose-6-phosphate synthase fused to a non-constitutive plant promoter, which allows for temporal, topological or stress-induced control over the expression of the gene. The invention can be used for protecting staple crop plants against drought, high salinity or temperature extremes and for improving the storage properties of harvested plants including green food stuffs, picked fruits and ornamental plants.
GB9627137A 1994-06-29 1995-06-29 Transgenic plants producing trehalose Expired - Fee Related GB2303856B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI943133A FI943133A0 (en) 1994-06-29 1994-06-29 Transgene vaexter
PCT/FI1995/000377 WO1996000789A1 (en) 1994-06-29 1995-06-29 Transgenic plants producing trehalose

Publications (3)

Publication Number Publication Date
GB9627137D0 GB9627137D0 (en) 1997-02-19
GB2303856A true GB2303856A (en) 1997-03-05
GB2303856B GB2303856B (en) 1998-12-30

Family

ID=8541026

Family Applications (1)

Application Number Title Priority Date Filing Date
GB9627137A Expired - Fee Related GB2303856B (en) 1994-06-29 1995-06-29 Transgenic plants producing trehalose

Country Status (11)

Country Link
EP (1) EP0763118A1 (en)
JP (1) JPH10501978A (en)
CN (1) CN1159833A (en)
AU (1) AU699391B2 (en)
CA (1) CA2193861A1 (en)
CZ (1) CZ294329B6 (en)
FI (1) FI943133A0 (en)
GB (1) GB2303856B (en)
HU (1) HU221613B (en)
NZ (1) NZ288635A (en)
WO (1) WO1996000789A1 (en)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6118047A (en) 1993-08-25 2000-09-12 Dekalb Genetic Corporation Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction
US5780709A (en) * 1993-08-25 1998-07-14 Dekalb Genetics Corporation Transgenic maize with increased mannitol content
IL116564A0 (en) * 1995-01-04 1996-03-31 Mogen Int Process for producing trehalose in plants
EP0784095A3 (en) 1996-01-12 1997-12-29 Mogen International N.V. Enhanced accummulation of trehalose in plants
IN1997CH00924A (en) 1996-05-03 2005-03-04 Syngenta Mogen Bv Regulating metabolism by modifying the level of trehalose-6-phosphate
MX205414B (en) * 1996-05-08 2001-12-07 Univ Mexico Nacional Autonoma METHOD OF INCREASING TREHALOSE IN ORGANISMS BY TRANSFORMATION OF SELAGINELLA LEPIDOPHYLIA TREHALOSE-6-PHOSPHATE SINTASE/PHOSPHATASE cDNA.
ES2110918B1 (en) * 1996-07-04 1998-08-01 Univ Poltecnica De Valencia OBTAINING TOLERANT PLANTS TO OSMOTIC STRESS THROUGH GENETIC MANIPULATION OF CARBOHYDRATE METABOLISM.
EP0905242A4 (en) * 1996-10-24 2001-11-07 Japan Tobacco Inc Method for controlling water content of plant
GB9703681D0 (en) * 1997-02-21 1997-04-09 Gene Shears Pty Ltd Protein complemention
TW466116B (en) 1997-03-04 2001-12-01 Hayashibara Biochem Lab Reduction inhibitory agent for active-oxygen eliminating activity, method for inhibiting the reduction of said activity, and composition containing said agent
HUP0002948A3 (en) * 1997-05-02 2002-09-30 Syngenta Mogen Bv Regulating metabolism by modifying the level of trehalose-6-phosphate by inhibiting endogenous trehalase levels
ZA989782B (en) 1997-10-30 1999-05-04 Mogen Int Pre-and postharvest inhibition of remobilisation of storage compounds
AU1559799A (en) * 1997-10-30 1999-05-24 Mogen International N.V. Novel high-fermenting microorganisms
AU2412499A (en) * 1997-10-30 1999-05-31 Mogen International N.V. Novel high-fermenting microorganisms
ZA989886B (en) * 1997-10-30 1999-04-30 Mogen Int Nuclear male sterile plants method of producing same and methods to restore fertility
KR100816113B1 (en) * 1998-01-22 2008-03-28 내셔날 리서치 카운실 오브 캐나다 Methods and Compositions for Modifying Levels of Secondary Metabolic Compounds in Plants
HUP0102598A3 (en) * 1998-03-11 2003-03-28 Syngenta Participations Ag Expression of trehalose biosynthetic genes in plants
DE19832334A1 (en) * 1998-07-17 2000-01-20 Norika Nordring Kartoffelzucht Use of a glucosylglycerol phosphate synthase gene for glucosylglycerol production and generation of transgenic plants with increased tolerance against environmental stress
EP1002867A1 (en) 1998-10-15 2000-05-24 K.U. Leuven Research & Development Specific genetic modification of the activity of trehalose-6-phosphate synthase and expression in a homologous or heterologous environment
GB0100105D0 (en) * 2001-01-04 2001-02-14 Leuven K U Res & Dev The use of plant TPS (Trehalose-6-phosphate syntase) as a selectable marker for plant transformation
KR100440725B1 (en) 2002-06-20 2004-07-15 주식회사 그린진 바이오텍 A Method for Increasing an Abiotic-Resistance in Monocot Plants
JP2006505279A (en) * 2002-11-06 2006-02-16 コーネル リサーチ ファンデーション， インコーポレーティッド TPS plant gene construct and transformant
EP2366790A1 (en) * 2004-04-20 2011-09-21 Syngenta Participations AG Regulatory sequences for expressing gene products in plant reproductive tissue
CN101586107A (en) * 2009-07-08 2009-11-25 四川农业大学 Gene sequence of trehalose-6-phosphate synthetase derived from selaginella pulvinata
CA2833765C (en) * 2011-04-22 2021-05-11 Danisco Us Inc. Filamentous fungi having an altered viscosity phenotype
CN113016524A (en) * 2019-12-24 2021-06-25 东北农业大学 Method for improving alkali resistance of rice
CN112980849B (en) * 2021-05-10 2022-10-18 长江师范学院 Active siRNA for preventing and treating clubroot by targeting plasmodiophora pbTPS1 gene and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0451896A1 (en) * 1990-03-28 1991-10-16 Gist-Brocades N.V. New yeast strains with enhanced trehalose content, process to obtain such yeasts and the use of these yeasts
WO1993017093A2 (en) * 1992-02-14 1993-09-02 Oy Alko Ab Increasing the trehalose content of organisms by transforming them with combinations of the structural genes for trehalose synthase
WO1995001446A1 (en) * 1993-06-30 1995-01-12 Mogen International N.V. Production of trehalose in plants
WO1995006126A1 (en) * 1993-08-24 1995-03-02 Mogen International N.V. Production of trehalose in plants

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0451896A1 (en) * 1990-03-28 1991-10-16 Gist-Brocades N.V. New yeast strains with enhanced trehalose content, process to obtain such yeasts and the use of these yeasts
WO1993017093A2 (en) * 1992-02-14 1993-09-02 Oy Alko Ab Increasing the trehalose content of organisms by transforming them with combinations of the structural genes for trehalose synthase
WO1995001446A1 (en) * 1993-06-30 1995-01-12 Mogen International N.V. Production of trehalose in plants
WO1995006126A1 (en) * 1993-08-24 1995-03-02 Mogen International N.V. Production of trehalose in plants

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Eur.J.Biochem. 1993,216(3),849-861 *
FEMS Microbiology Letters 1992,94(3),271-276 *

Also Published As

Publication number Publication date
EP0763118A1 (en) 1997-03-19
GB9627137D0 (en) 1997-02-19
CZ378296A3 (en) 1998-03-18
AU699391B2 (en) 1998-12-03
GB2303856B (en) 1998-12-30
NZ288635A (en) 1998-12-23
WO1996000789A1 (en) 1996-01-11
FI943133A0 (en) 1994-06-29
HU221613B (en) 2002-11-28
HUP9603608D0 (en) 1997-02-28
HUT75659A (en) 1997-05-28
CA2193861A1 (en) 1996-01-11
AU2794495A (en) 1996-01-25
CN1159833A (en) 1997-09-17
CZ294329B6 (en) 2004-12-15
JPH10501978A (en) 1998-02-24

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Legal Events

Date Code Title Description
732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20050629