GB2248619A - Improvements relating to the radio-labelling of proteins - Google Patents

Improvements relating to the radio-labelling of proteins

Info

Publication number
GB2248619A
GB2248619A GB9119528A GB9119528A GB2248619A GB 2248619 A GB2248619 A GB 2248619A GB 9119528 A GB9119528 A GB 9119528A GB 9119528 A GB9119528 A GB 9119528A GB 2248619 A GB2248619 A GB 2248619A
Authority
GB
United Kingdom
Prior art keywords
labelled
thiotriphosphates
labelling
proteins
radio
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB9119528A
Other versions
GB2248619B (en
GB9119528D0 (en
Inventor
Brian Maurice John Foxwell
Peter Parker
Andrew Malcolm Creighton
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nat Research & Dev Corp
Original Assignee
Nat Research & Dev Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nat Research & Dev Corp filed Critical Nat Research & Dev Corp
Publication of GB9119528D0 publication Critical patent/GB9119528D0/en
Publication of GB2248619A publication Critical patent/GB2248619A/en
Application granted granted Critical
Publication of GB2248619B publication Critical patent/GB2248619B/en
Anticipated expiration legal-status Critical
Revoked legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04—Organic compounds
    • A61K51/0491—Sugars, nucleosides, nucleotides, oligonucleotides, nucleic acids, e.g. DNA, RNA, nucleic acid aptamers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04—Organic compounds
    • A61K51/08—Peptides, e.g. proteins, carriers being peptides, polyamino acids, proteins
    • A61K51/10—Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody
    • A61K51/1093—Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody conjugates with carriers being antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2121/00—Preparations for use in therapy

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Veterinary Medicine (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Labeling Devices (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Nucleoside thiotriphosphates carrying <32>P in the gamma-thiophosphate group, prepared by reaction between a nucleoside diphosphate and a <32>P labelled thiophosphate salt, are used as thiophosphorylating agents for antibodies that bind to tumour-associated antigens. The labelled thiotriphosphates can be used to introduce a therapeutically useful <32>P atom into an antibody that has been modified by the incorporation into its structure of a peptide region capable of acting as a substrate for a phosphokinase. The resulting labelled antibody can then be used for injection in anti-tumour therapy and has clinical advantages over the corresponding phosphorylated analogue.
GB9119528A 1989-03-23 1991-09-11 Improvements relating to the radio-labelling of proteins Revoked GB2248619B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB898906708A GB8906708D0 (en) 1989-03-23 1989-03-23 Improvements relating to the radiolabelling of proteins

Publications (3)

Publication Number Publication Date
GB9119528D0 GB9119528D0 (en) 1991-12-04
GB2248619A true GB2248619A (en) 1992-04-15
GB2248619B GB2248619B (en) 1993-02-24

Family

ID=10653894

Family Applications (2)

Application Number Title Priority Date Filing Date
GB898906708A Pending GB8906708D0 (en) 1989-03-23 1989-03-23 Improvements relating to the radiolabelling of proteins
GB9119528A Revoked GB2248619B (en) 1989-03-23 1991-09-11 Improvements relating to the radio-labelling of proteins

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GB898906708A Pending GB8906708D0 (en) 1989-03-23 1989-03-23 Improvements relating to the radiolabelling of proteins

Country Status (8)

Country Link
EP (1) EP0464101B1 (en)
JP (1) JP2942776B2 (en)
AT (1) ATE114315T1 (en)
DE (1) DE69014300T2 (en)
DK (1) DK0464101T3 (en)
ES (1) ES2065525T3 (en)
GB (2) GB8906708D0 (en)
WO (1) WO1990011289A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5583212A (en) * 1989-03-23 1996-12-10 British Technology Group Limited (gamma)-[32 p](gamma) -thioribonucleoside-51 -triphosphates
GB9103886D0 (en) * 1991-02-25 1991-04-10 Creighton Andrew 32p-thiophosphate preparation
GB9126650D0 (en) * 1991-12-16 1992-02-12 Creighton Andrew M Further improvements relating to the radiolabelling of proteins
IL115177A0 (en) * 1994-09-16 1995-12-31 Immunomedics Inc Phosphorus-32 labeling of antibodies for cancer therapy
US5728369A (en) * 1994-10-05 1998-03-17 Immunomedics, Inc. Radioactive phosphorus labeling of proteins for targeted radiotherapy
WO1996023816A1 (en) * 1995-02-01 1996-08-08 British Technology Group Limited Radiolabelled proteins
US6187286B1 (en) * 1996-12-27 2001-02-13 The General Hospital Corporation Tumor imaging agents, methods and kits

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2161484A (en) * 1984-07-02 1986-01-15 Du Pont 32p-labelled nucleotides
EP0214014A1 (en) * 1985-08-01 1987-03-11 Centre National De La Recherche Scientifique (Cnrs) Kit for the enzymatic labelling of nucleoside triphosphates, and process for the preparation of nucleoside triphosphates labelled with 32P or 35S with 32PO4H3 or 35S-PO3Na3
GB2186579A (en) * 1986-01-21 1987-08-19 Brian Maurice John Foxwell Improvements relating to the radio-labelling of proteins

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2161484A (en) * 1984-07-02 1986-01-15 Du Pont 32p-labelled nucleotides
EP0214014A1 (en) * 1985-08-01 1987-03-11 Centre National De La Recherche Scientifique (Cnrs) Kit for the enzymatic labelling of nucleoside triphosphates, and process for the preparation of nucleoside triphosphates labelled with 32P or 35S with 32PO4H3 or 35S-PO3Na3
GB2186579A (en) * 1986-01-21 1987-08-19 Brian Maurice John Foxwell Improvements relating to the radio-labelling of proteins

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Accounts of Chemical Research, vol. 12 no.6 (1979) 204-210 *
Biochimica et Biophysica Acta, vol.526 (1979) 11-31 *
Biochimica et Biophysica Acta, vol.565 (1979) 209-213 *

Also Published As

Publication number Publication date
DK0464101T3 (en) 1994-12-19
GB2248619B (en) 1993-02-24
GB9119528D0 (en) 1991-12-04
EP0464101A1 (en) 1992-01-08
WO1990011289A1 (en) 1990-10-04
JP2942776B2 (en) 1999-08-30
DE69014300T2 (en) 1995-04-13
EP0464101B1 (en) 1994-11-23
JPH04503809A (en) 1992-07-09
DE69014300D1 (en) 1995-01-05
ATE114315T1 (en) 1994-12-15
ES2065525T3 (en) 1995-02-16
GB8906708D0 (en) 1989-05-10

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Legal Events

Date Code Title Description
732 Registration of transactions, instruments or events in the register (sect. 32/1977)
773K Patent revoked under sect. 73(2)/1977