GB2228081B - A fluorimeter, and a method of carrying out a fluorescent assay of a plurality of analytes - Google Patents

A fluorimeter, and a method of carrying out a fluorescent assay of a plurality of analytes

Info

Publication number
GB2228081B
GB2228081B GB8828476A GB8828476A GB2228081B GB 2228081 B GB2228081 B GB 2228081B GB 8828476 A GB8828476 A GB 8828476A GB 8828476 A GB8828476 A GB 8828476A GB 2228081 B GB2228081 B GB 2228081B
Authority
GB
United Kingdom
Prior art keywords
fluorimeter
analytes
carrying
fluorescent assay
assay
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
GB8828476A
Other versions
GB8828476D0 (en
GB2228081A (en
Inventor
James N Miller
Nichola J Seare
Tony E Edmonds
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Loughborough Consultants Ltd
Original Assignee
Loughborough Consultants Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Loughborough Consultants Ltd filed Critical Loughborough Consultants Ltd
Priority to GB8828476A priority Critical patent/GB2228081B/en
Publication of GB8828476D0 publication Critical patent/GB8828476D0/en
Publication of GB2228081A publication Critical patent/GB2228081A/en
Priority to GB9220976A priority patent/GB2258728B/en
Application granted granted Critical
Publication of GB2228081B publication Critical patent/GB2228081B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J1/00—Photometry, e.g. photographic exposure meter
    • G01J1/10—Photometry, e.g. photographic exposure meter by comparison with reference light or electric value provisionally void
    • G01J1/16—Photometry, e.g. photographic exposure meter by comparison with reference light or electric value provisionally void using electric radiation detectors
    • G01J1/1626—Arrangements with two photodetectors, the signals of which are compared
    • G01J2001/1636—Arrangements with two photodetectors, the signals of which are compared one detector directly monitoring the source, e.g. also impulse time controlling
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N2021/1748—Comparative step being essential in the method
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G01N2021/6471—Special filters, filter wheel
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6484—Optical fibres
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6445—Measuring fluorescence polarisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/04—Batch operation; multisample devices

Landscapes

  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
GB8828476A 1988-12-06 1988-12-06 A fluorimeter, and a method of carrying out a fluorescent assay of a plurality of analytes Expired - Fee Related GB2228081B (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
GB8828476A GB2228081B (en) 1988-12-06 1988-12-06 A fluorimeter, and a method of carrying out a fluorescent assay of a plurality of analytes
GB9220976A GB2258728B (en) 1988-12-06 1992-10-06 A fluorimeter

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB8828476A GB2228081B (en) 1988-12-06 1988-12-06 A fluorimeter, and a method of carrying out a fluorescent assay of a plurality of analytes

Publications (3)

Publication Number Publication Date
GB8828476D0 GB8828476D0 (en) 1989-01-05
GB2228081A GB2228081A (en) 1990-08-15
GB2228081B true GB2228081B (en) 1993-07-07

Family

ID=10648053

Family Applications (1)

Application Number Title Priority Date Filing Date
GB8828476A Expired - Fee Related GB2228081B (en) 1988-12-06 1988-12-06 A fluorimeter, and a method of carrying out a fluorescent assay of a plurality of analytes

Country Status (1)

Country Link
GB (1) GB2228081B (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6025985A (en) 1997-07-16 2000-02-15 Ljl Biosystems, Inc. Moveable control unit for high-throughput analyzer
US6097025A (en) 1997-10-31 2000-08-01 Ljl Biosystems, Inc. Light detection device having an optical-path switching mechanism
US6317207B2 (en) 1999-02-23 2001-11-13 Ljl Biosystems, Inc. Frequency-domain light detection device
US6466316B2 (en) 1998-07-27 2002-10-15 Ljl Biosystems, Inc. Apparatus and methods for spectroscopic measurements
US6469311B1 (en) 1997-07-16 2002-10-22 Molecular Devices Corporation Detection device for light transmitted from a sensed volume
US6483582B2 (en) 1998-07-27 2002-11-19 Ljl Biosystems, Inc. Apparatus and methods for time-resolved spectroscopic measurements
US6498335B2 (en) 1998-02-20 2002-12-24 Ljl Biosystems, Inc. Broad range light detection system
US6576476B1 (en) 1998-09-02 2003-06-10 Ljl Biosystems, Inc. Chemiluminescence detection method and device

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2672128B1 (en) * 1991-01-28 1995-08-18 Cis Bio Int METHOD FOR MEASURING THE LUMINESCENCE EMITTED IN A LUMINESCENCE ASSAY.
US6352672B1 (en) 1991-01-28 2002-03-05 Cis Bio International Apparatus for measuring the luminescence emitted in a luminescent assay
US6861264B2 (en) 1992-01-27 2005-03-01 Cis Bio International Method of measuring the luminescence emitted in a luminescent assay
US6825921B1 (en) 1999-11-10 2004-11-30 Molecular Devices Corporation Multi-mode light detection system

Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1034435A (en) * 1962-03-26 1966-06-29 Beckman Instruments Inc Double beam discharge lamp
US3973129A (en) * 1975-01-10 1976-08-03 Bell Telephone Laboratories, Incorporated Fluorimetric apparatus and method for analysis of body fluid
GB1458733A (en) * 1973-04-16 1976-12-15 Hitachi Ltd Spectrophotometers
GB2044924A (en) * 1979-02-16 1980-10-22 Hitachi Ltd Spectrofluorometer
US4320970A (en) * 1975-11-24 1982-03-23 Dowben Robert M Photon counting fluorimeter
WO1983003900A1 (en) * 1982-04-23 1983-11-10 Pharmacontrol Corp. Apparatus for use in spectroscopically analyzing properties of test samples
US4577109A (en) * 1980-10-06 1986-03-18 Regents Of The University Of California Remote multi-position information gathering system and method
EP0257559A2 (en) * 1986-08-21 1988-03-02 Becton, Dickinson and Company Multi-color fluorescence analysis with single wavelength excitation
GB2196734A (en) * 1986-09-30 1988-05-05 Astromed Ltd Multiple assaying technique
EP0289861A2 (en) * 1987-05-07 1988-11-09 Becton, Dickinson and Company Detector assembly for analyzer instrument
US4822127A (en) * 1986-06-16 1989-04-18 Shiley Incorporated Multi-channel optical transmission system
EP0355738A2 (en) * 1988-08-26 1990-02-28 Hitachi, Ltd. Fluorophotometer for use in automatic equipment of chemical analysis for measuring the intensity of fluorescence in the automatic equipment

Patent Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1034435A (en) * 1962-03-26 1966-06-29 Beckman Instruments Inc Double beam discharge lamp
GB1458733A (en) * 1973-04-16 1976-12-15 Hitachi Ltd Spectrophotometers
US3973129A (en) * 1975-01-10 1976-08-03 Bell Telephone Laboratories, Incorporated Fluorimetric apparatus and method for analysis of body fluid
US4320970A (en) * 1975-11-24 1982-03-23 Dowben Robert M Photon counting fluorimeter
GB2044924A (en) * 1979-02-16 1980-10-22 Hitachi Ltd Spectrofluorometer
US4577109A (en) * 1980-10-06 1986-03-18 Regents Of The University Of California Remote multi-position information gathering system and method
WO1983003900A1 (en) * 1982-04-23 1983-11-10 Pharmacontrol Corp. Apparatus for use in spectroscopically analyzing properties of test samples
US4822127A (en) * 1986-06-16 1989-04-18 Shiley Incorporated Multi-channel optical transmission system
EP0257559A2 (en) * 1986-08-21 1988-03-02 Becton, Dickinson and Company Multi-color fluorescence analysis with single wavelength excitation
GB2196734A (en) * 1986-09-30 1988-05-05 Astromed Ltd Multiple assaying technique
EP0289861A2 (en) * 1987-05-07 1988-11-09 Becton, Dickinson and Company Detector assembly for analyzer instrument
EP0355738A2 (en) * 1988-08-26 1990-02-28 Hitachi, Ltd. Fluorophotometer for use in automatic equipment of chemical analysis for measuring the intensity of fluorescence in the automatic equipment

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6025985A (en) 1997-07-16 2000-02-15 Ljl Biosystems, Inc. Moveable control unit for high-throughput analyzer
US6033100A (en) 1997-07-16 2000-03-07 Ljl Biosystems, Inc. Floating head assembly
US6071748A (en) 1997-07-16 2000-06-06 Ljl Biosystems, Inc. Light detection device
US6159425A (en) 1997-07-16 2000-12-12 Ljl Biosystems, Inc. Sample transporter
US6187267B1 (en) 1997-07-16 2001-02-13 Ljl Biosystems, Inc. Chemiluminescence detection device
US6499366B1 (en) 1997-07-16 2002-12-31 Ljl Biosystems, Inc. Sample feeder
US6469311B1 (en) 1997-07-16 2002-10-22 Molecular Devices Corporation Detection device for light transmitted from a sensed volume
US6097025A (en) 1997-10-31 2000-08-01 Ljl Biosystems, Inc. Light detection device having an optical-path switching mechanism
US6498335B2 (en) 1998-02-20 2002-12-24 Ljl Biosystems, Inc. Broad range light detection system
US6483582B2 (en) 1998-07-27 2002-11-19 Ljl Biosystems, Inc. Apparatus and methods for time-resolved spectroscopic measurements
US6466316B2 (en) 1998-07-27 2002-10-15 Ljl Biosystems, Inc. Apparatus and methods for spectroscopic measurements
US6576476B1 (en) 1998-09-02 2003-06-10 Ljl Biosystems, Inc. Chemiluminescence detection method and device
US6317207B2 (en) 1999-02-23 2001-11-13 Ljl Biosystems, Inc. Frequency-domain light detection device

Also Published As

Publication number Publication date
GB8828476D0 (en) 1989-01-05
GB2228081A (en) 1990-08-15

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Legal Events

Date Code Title Description
732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)
PCNP Patent ceased through non-payment of renewal fee