GB2207127A - Process for biological purification of waste waters containing methanol - Google Patents

Process for biological purification of waste waters containing methanol Download PDF

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Publication number
GB2207127A
GB2207127A GB8716573A GB8716573A GB2207127A GB 2207127 A GB2207127 A GB 2207127A GB 8716573 A GB8716573 A GB 8716573A GB 8716573 A GB8716573 A GB 8716573A GB 2207127 A GB2207127 A GB 2207127A
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GB
United Kingdom
Prior art keywords
waste waters
methanol
strain
purification
culturing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB8716573A
Other versions
GB2207127B (en
GB8716573D0 (en
Inventor
Petr Iliich Gvozdyak
Alexei Dmitrievich Denis
Natalya Fedoseevna Mogilevich
Mark Benyaminovich Tsinberg
Nadezhda Ivanovna Grischenko
Olga Nikolaevna Erzikova
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Volgo Ural Ni I Pi Dobyche I P
Original Assignee
Volgo Ural Ni I Pi Dobyche I P
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to SU833589002A priority Critical patent/SU1150892A1/en
Priority to DD87301124A priority patent/DD281716A7/en
Priority to AT0157187A priority patent/AT392060B/en
Priority to FR8709638A priority patent/FR2617830B1/en
Priority to JP17373987A priority patent/JPS6418497A/en
Priority to IN542/CAL/87A priority patent/IN167828B/en
Priority to GB8716573A priority patent/GB2207127B/en
Application filed by Volgo Ural Ni I Pi Dobyche I P filed Critical Volgo Ural Ni I Pi Dobyche I P
Priority to DE19873723422 priority patent/DE3723422A1/en
Priority to CN87105219.9A priority patent/CN1015889B/en
Publication of GB8716573D0 publication Critical patent/GB8716573D0/en
Publication of GB2207127A publication Critical patent/GB2207127A/en
Application granted granted Critical
Publication of GB2207127B publication Critical patent/GB2207127B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/02—Aerobic processes
    • C02F3/12—Activated sludge processes
    • C02F3/1205—Particular type of activated sludge processes
    • C02F3/1231—Treatments of toxic sewage
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/28—Anaerobic digestion processes
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Water Supply & Treatment (AREA)
  • Hydrology & Water Resources (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Description

2207 127 1-1 PROCESS tOR BIOLOGICAL PURIFICATION OF WASTE WATERS
CONTAINING METHANOL The present invention relates to biological purification of waste waters and, more particularly, to a pro- cess for purification of waste waters from methanol.
The present invention is useful in enterprises effectuating extraction and processing of sulphur-containing natural gas.
Known in the art is culturing of microorganisms 1C) pertaining to various physiological groups on nutrient media containing methanol as a carbon source.
This invention resides in that in the process for biological purification of waste waters from methanol effected by culturing a microorganism on waste waters pre- liminarily neutralized with orthophosphoric acid and enriched with sources of nitrogen prior to decontamination of waste waters from methanol, according to the present invention use is made of the strain Metaylomonas metanica B-2576 deposited at the Central-Museum of Industrial Microorga- nisms of the All-Union Research Institute of Genetics and Selection of Industrial Microorganisms (deposited 04.07.
1982, accession No.B-2576) as the microorganism.
Owing to the present invention the rate of consumption of the required reagents for purification of 1 j of waste waters is reduced by 12 times as compared to the prior art process of purification of waste waters along with a 2-times intensification of the process.
According to the present invention, it is advisable f - 2 to preliminarily carry out neutralization of the waste waters with orthophosphoric ac.d to a pH value of 5.5-7.0 and culturing of the above-specified microorganism at a temperature within the range of from 20 to 37 0 C.
Further objects and advantages of the present inven tion will now become more fully apparent from the follow ing detailed description of the process for purification of waste waters and specific examples illustrating its particular embodiments.
A methanol-assimilating"culture is recovered for a practical implementation of the process according to the present invention. To this end, a soil contaminated with methanol is placed into a vertical glass column and a methanol-containing waste water from gas fields preliminarily diluted with a nutrient medium to the concentra, - tdon of methanol of 1 g-/1 is passed therethrough. Use is made of a mineral nutrient medium containing, g/dm3:
K 2 HPO 4 - 1.09 MgS04 & 7H 2 0 - 0.21 FeSO4 7H20 - 0.039 NaCl - 0.2g NH 4N03 - 0.5; pH = 7.0. Concentration of methanol in the medium is gradually increased to 10.0 g/dm3 while diminishing the dilution of the real waste water with the nutrient medium. Increasing of the concentration of methanol in the waste water is effected upon achievement of its full utilization from the waste water of the previous dilution. At the stage of the supply of the waste water containing 10.0 g/dM3 of methanol and at the absence thereof in the waste water effluent from the column, the t 1 recovery of the pure culture is effected by inoculating the contents of the column onto an agarized mineral nutrient-medium of the abovespecified composition and containing 2.0 g/dm3 of methanol. Under stationary conditions the culture consumes methanol in lower concentrations as compared to growing thereof under continuous-flow conditions. The growth of the microorganism on a medium containing 2.0 g/dM3 of methanol demonstrates its utilization by the culture since methanol is a sole source of carbon.
The strain Methylomonas metanica B-2576 has the following cultural-morphological and physiologo-biochemical characteristics: cells straight, bacilliform, mobile, have a polar flagellum, gram-negative, aerobicq multiply by division, cell size - 0.6- 1 mm; colonies have a pink or creamy colour. slimy, colonies of 3-4 mm size.
This strain does not grow on conventional nutrient media such as meatpeptone agar; it grows exceptionally on methanol-containing media.
The isolated commercial culture Methylomonas metanica B-2576 is deposited at the Central Museum of IndusLrial Microorganisms of the All-Union Researca Institute of Genetics and Selection of 1ndustrial Microorganisms (04.07.82).
The process for biological purification of waste waters from methanol according to the present invention resides in that methanol-containing waste waters are neutralized with orthophosphoric acid to a pH of 5.5-7.0 and enriched with sources of nitrogen by introducing, e.g., ammonium nitrate.
X Then the waste waters are added with the strain Methylomonas metanica B-2576 and culturing of tle above- mentioned microorganism is effected at a temperature of from 20 to 37 0 C. As it has been shown in investigations, the culturing of the above-specified microorganism at a temperature within the range of from 20 to 37 OC ensures i utilization of methanol by bacteria to trace amounts or even to a total absence of methanol in waste waters. At a temperature below 20 0 C the process of utilization of metha- nol by the microorganism gets substantially delayed and over 48 hours of the culturing bacteria consume only 450 mg of methanol from 1 litre of waste waters. At tem- i 1 peratures above 37 0 C the strain Methylomonas metanica B-2576 does not grow and does not utilize methanol.
1 The waste waters are preliminarily neutralized with 9 orthoptiosphoric acid to a pH of not less than 5.5, since at a lower value of pH the growth and assimilation of methanol by the employed strain Methylomonas metanica B-2576 are decreased. A slightly alkaline pH value of the waste 1 1 waters (pH above 7.0) is neither favourable for a total consumption of methanol by the above-specified microorga- nism.
The process for biological purification of waste waters makes it possible to decontaminate waste waters containing methanol in ah high concentration - 55Vo by mass.
Methanol is fully assimildted by the microorganism and the purified water does not contain it at all, or only trace amounts of methanol are present taerein.
1 1 il 1 i - 5 Owing to the use of the strain Methylomonas metanica B-2576 instead of previously employed yeast culture of the species Hansenula polymorpha in purification of waste waters the decontamination process'becomes economically more efficient because of the fact that consumption of orthophosphoric, acid for neutralization of waste waters is considerably reduced not to a pH of 3.9-4.0, but to a pH of from 5.5 to 7.0; secondly, for the development and assimilation of methanol the strain Methylomonas meta- nica,B-2576 necessitates no such vitamins as thiamin and desthiobiotin at all.
A shorter time of residence of waste waters in the purification apparatus, as compared to that in the Drior art process, makes it possible to intensify the purifica- tion process by more ta ' an two times.
As a result of purification of waste waters by the process according to the present invention a bacterial biomass is accumulated which contains wore than 70% of crude protein employed further for the preparation of various protein-dontaining products.
Example 1
Into a fermenter of 5 1 capacity employed for a continuous culturing of microorganisms waste waters resulting from extraction and processing of a hydrogen-sulphide containing natural gas and containing 50.0 g/dm3 of methanol are introduced. Then orklophospnoric acid is added thereto in the amount of 0. 4 ml/dm3 along with ammonium 3 nitrate in the amount of 150.mg/dm The waste waters are preliminarily diluted with a synthetic nutrient medium to the concentration of m.thianol of 10 g/dm3 and the ermenter content is inoculated with the strain Methylomonas metanica B-2576 grown in Erlenmeyer flasks under shaking on a synthetic nutrient medium containing methanol in the concentration of 5.0 g/dm The c.oncentration of methanol in the waste waters is gradually increased to 50.0 g/dm3 by decreasing its dilution with the synthetic nutrient medium. The concentration of orthophosphoric acid in the waste waters is maintained at 0.4 ml/dm3 (pH = 6.5). The temperature of the waste waters is 30 OC. When the concentration of the strain Methylomonas metanica B-2576 in the fermenter reaches 35+5 g/dm 3, the growing of the bacterial culture is ef- fected in a direct flow of the waste water with the dilution coefficient of 0.2 h71. which co rresponds to the time of residence of the purified waste waters in the fermenter of 5 hours.
During 10 days of a continuous culturing of the strain Methylomonas metanica B-2576 on methanol-containing waste waters the biomass of the microorganism is stably maintained at the level of 37+2 g/dm3 which points to a high degree of assimilation of methanol by the microorganism. The purified waste waters contain methanol in trace concentrations.
Example 2
The process of purification of methanol-containing waste waters is effected in a manner similar to that d z A.9 if 1 1, t - 7 described in the foregoing Example 1. However, the preliminary neuuralization of waste waters is effected by means of orthophosphoric acid to the pH of 5.5. The waste waters are kept at the temperature of 200 G.
The culturing of the strain Methylomonas metanica B-2576 is conducted under discontinuous process conditions at 3 the optimal initial concentration of methanol of 1 g/dm The purification process is carried out for 48 hours under vigorous stirring.
On expiration of 48 hours of culturing'of tne above- specified microorganism the waste waters contain 0.002 g/dm3 of methanol.
Example 3
The process of purification of waste waters is car- ried out in a manner similar to that described in Example 1 hereinbefore. However. tne preliminary neutralization of the waste waters is effected by means of orthopriosphoric acid to the pH of 7.0. The waste waters are maintained at the temperature of 370C.
The culturing of the strain Ylethylomonas meta- nica B-2576 is conducted under discontinuous process conditions at the initial concentration of methanol of 1 g/dm3 The purification process is carried out for 48 hours under vigorous stirring. On expiration of 48 hours of culturing of the above-specified microorganism the purified waste waters do not contain metnanol.
h

Claims (4)

1. A process of biological purification of waste waters from methanol comprising neutralization of the i waste waters with orthophospluoric acid, enrichment of the 1 i waste waters with nitrogen sources; introduction of the i strain,Methylomonas metanica B-2576 -deposited at the Central Museum of Industrial Microorganisms of the All-Union Research Institute of Genetics and Selection of Indust- rial microorganisms; culturing said strain on enriched waste waters till purification thereof from methanol.
i i j i i i
2. A process according to Claim 1, wherein said neutralization of the waste waters with orthophosphoric acid is effected to a pH within the range of from 5.5 to 7.0.
i
3. A process according to Claim 19 wherein said culturing of the strain Methylomonas metanica B-2576 is carried out at a temperature within the range of from 20 to 37 OC.
1
4. A process of biological purification of waste waters from methanol according to any of the foregoing Claims substantially as described in the Specification and Examples hereinbefore.
i Published 1988 at The Patent Office. State ilwase. 6671 High Holborn, London WC1R 4TP- Further copies may be obtained from The Patent Sales Branch, St Mary Cray. Orpingtorl, Kent BR5 3RD. Printed by Multiplex techniques ltd, St Mary Cray, Kent. Con. 1187 Olnoe.
GB8716573A 1983-05-06 1987-07-14 Process for biological purification of waste waters containing methanol Expired - Fee Related GB2207127B (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
SU833589002A SU1150892A1 (en) 1983-05-06 1983-05-06 Method of biochemical purification of waste water of methanol
DD87301124A DD281716A7 (en) 1983-05-06 1987-03-26 PROCESS FOR BIOCHEMICAL METHANOL REMOVAL FROM WASTEWATERS
AT0157187A AT392060B (en) 1983-05-06 1987-06-22 METHOD FOR THE BIOLOGICAL PURIFICATION OF METHANOL-CONTAINING WASTEWATERS
FR8709638A FR2617830B1 (en) 1983-05-06 1987-07-07 PROCESS FOR THE BIOLOGICAL PURIFICATION OF METHANOL IN WASTEWATER
JP17373987A JPS6418497A (en) 1983-05-06 1987-07-11 Biological purifying method regarding methanol of waste water
GB8716573A GB2207127B (en) 1983-05-06 1987-07-14 Process for biological purification of waste waters containing methanol
IN542/CAL/87A IN167828B (en) 1983-05-06 1987-07-14
DE19873723422 DE3723422A1 (en) 1983-05-06 1987-07-15 METHOD FOR THE BIOLOGICAL PURIFICATION OF WASTE WATER FROM METHANOL
CN87105219.9A CN1015889B (en) 1983-05-06 1987-07-30 Remove the biological purification method of methyl alcohol in the sewage

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SU833589002A SU1150892A1 (en) 1983-05-06 1983-05-06 Method of biochemical purification of waste water of methanol
GB8716573A GB2207127B (en) 1983-05-06 1987-07-14 Process for biological purification of waste waters containing methanol

Publications (3)

Publication Number Publication Date
GB8716573D0 GB8716573D0 (en) 1987-08-19
GB2207127A true GB2207127A (en) 1989-01-25
GB2207127B GB2207127B (en) 1991-01-23

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Family Applications (1)

Application Number Title Priority Date Filing Date
GB8716573A Expired - Fee Related GB2207127B (en) 1983-05-06 1987-07-14 Process for biological purification of waste waters containing methanol

Country Status (7)

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JP (1) JPS6418497A (en)
AT (1) AT392060B (en)
DE (1) DE3723422A1 (en)
FR (1) FR2617830B1 (en)
GB (1) GB2207127B (en)
IN (1) IN167828B (en)
SU (1) SU1150892A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2663797C2 (en) * 2016-07-13 2018-08-09 Общество с ограниченной ответственностью "Газпром нефтехим Салават" (ООО "Газпром нефтехим Салават") Method of oil refinery and petrochemical production wastewater treatment from methanol
RU2768939C1 (en) * 2020-12-16 2022-03-25 Николай Борисович Марков Method for biological treatment of highly concentrated waste water from methanol

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1420445A (en) * 1972-03-20 1976-01-07 Marabon Ab Process and a medium for the cultivation of microorganisms

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2045748A (en) * 1979-03-09 1980-11-05 Ici Ltd Biotransformations using methane-utilizing bacteria
JPS61162171A (en) * 1985-01-09 1986-07-22 チバ‐ガイギー　アクチエンゲゼルシヤフト Hypomicrobium microorganism and its use

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1420445A (en) * 1972-03-20 1976-01-07 Marabon Ab Process and a medium for the cultivation of microorganisms

Also Published As

Publication number Publication date
DE3723422A1 (en) 1989-01-26
ATA157187A (en) 1990-07-15
GB2207127B (en) 1991-01-23
GB8716573D0 (en) 1987-08-19
IN167828B (en) 1990-12-29
AT392060B (en) 1991-01-25
FR2617830A1 (en) 1989-01-13
JPS6418497A (en) 1989-01-23
SU1150892A1 (en) 1988-05-15
FR2617830B1 (en) 1989-12-15

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PCNP Patent ceased through non-payment of renewal fee

Effective date: 19920714