GB2194886A - Medicaments containing tissue plasminogen activator - Google Patents

Abstract

Tissue plasminogen activator (t-PA), optionally together with superoxide dismutase (SOD), is used to manufacture a medicament for use in the inhibition of damage to jeopardised tissue during blood reperfusion. The medicament is for preventing damage to tissue following ischaemic attack, e.g. in myocardial tissue, when blood supply is restored.

Description

1 GB2194886A 1

SPECIFICATION

Novel pharmaceutical use 1.

1 15 1 The present invention relates to tissue plasminogen activator, to its combination with superoxide 5 dismutase, to pharmaceutical formulations thereof, and to the use thereof in human and veteri nary medicine.

There exists a dynamic equilibrium between the enzyme system capable of forming blood clots, the coagulation system, and the enzyme system capable of dissolving blood clots, the fibrinolytic system, which maintains an intact patent vascular bed. To limit loss of blood from 10 injury, blood clots are formed in the injured vessel. After natural repair of the injury, the superfluous blood clots are dissolved through operation of the fibrinolytic system. Occasionally, blood clots form without traumatic injury and may lodge in major blood vessels resulting in a partial or even total obstruction to blood flow. When this occurs in the heart, lung or brain, the result may be a myocardial infarction, pulmonary embolism or stroke. These conditions combined 15 are the leading cause of morbidity and mortality in the industrialised nations.

Blood clots consist of a fibrous network that is capable of dissolution by the proteolytic enzyme plasmin. The enzyme is derived from the inactive proenzyme, plasminogen, a component of blood plasma, by the action of a plasminogen activator. There are two immunologically distinct mammalian plasminogen activators. Intrinsic plasminogen activator, also known as uroki- 20 nase, is an enzyme produced by the kidney and can be isolated from urine. It can also be prepared from a number of tissue culture sources. Extrinsic plasminogen activator, also known as vascular plasminogen activator and as tissue plasminogen activator (t- PA), can be isolated from many tissue homogenates (notably human uterus), the vascular cell wall and from some cell cultures. In addition to these two kinds of plasminogen activator, there is also a bacterial 25 product, streptokinase, prepared from beta-haemolytic streptococci. A major drawback with both urokinase and streptokinase is that they are active throughout the circulation and not just at the site of a blood clot. They can, for example, destroy other blood proteins, such as fibrinogen, prothrombin, factor V and factor VIII so reducing blood clotting ability and increasing the risk of haemorrhage. In contrast, the biological activity of t-PA is dependent on the presence of fibrin to 30 which it binds and where it is activated. Maximum activity is thus developed only at the site of a blood clot, i.e. in the presence of the fibrin network to be dissolved, and this greatly avoids the risk of haemorrhage.

The interruption of blood flow in a vessel generally leads to the onset of an ischaernic event.

In this condition the tissue is deprived of oxygen and becomes jeopardized, a state in which the 35 tissue is injured but still potentially viable. If however the condition is maintained for a period of, say, three of more hours, the tissue becomes necrotic and, once in this state, cannot be recovered. It is therefore important that reperfusion, i.e. the restoration of blood flow, takes place as soon as possible to salvage the tissue before it becomes permanently damaged.

Ironically, reperfusion itself, even if carried out before the tissue becomes necrotic, results in a 40 complex group of phenomena, including the putative formation of the superoxide radical, that have a deleterious effect on hypoxic tissue. Consequently, reperfusion can lead only to the partial recovery of jeopardized tissue, the remainder being permanently damaged by the occur rence of one or more of these phenomena.

It has now been found that t-PA inhibits the damage to jeopardized tissue during reperfusion by protecting it against one or more of the aforementioned phenomena. The mechanism of action of t-PA in affording such protection has not been elucidated but it is independent of its action as a thrombolytic agent. This newly discovered property thus enables t-PA, or a pharma ceutical formulation thereof, to be used as an inhibitor of damage to jeopardized tissue in the circumstances outlined herein. Accordingly, the present invention provides:

(a) A method for inhibiting damage to jeopardized tissue during reperfusion in a mammal, which comprises administering to the mammal an effective amount of t-PA; (b) Use of t-PA in inhibiting the damage to jeopardized tissue during reperfusion in a mammal; (c) Use of t-PA for the manufacture of a medicament for the inhibition of damage to jeopardized tissue during reperfusion in a mammal; or (d) A pharmaceutical formulation for use in inhibiting damage to jeopardized tissue during reper fusion in a mammal, which comprises t-PA and a pharmaceutically acceptable carrier.

Although the present invention may be used to protect any jeopardized tissue, it is particularly useful in inhibiting damage to jeopardized myocardial tissue.

The t-PA of use with the present invention may be any bioactive protein substantially corre- 60 sponding to mammalian, and especially human, t-PA and includes forms with and without glycosylation. It may be one- or two-chain t-PA, or a mixture thereof, as described in EP-A-1 12 122, and, in the case of fully glycosylated human t-PA, has an apparent molecular weight on polyacrylamide gel of about 70,000 and an isoelectric point of between 7. 5 and 8.0. Preferably the t-PA has a specific activity of about 500,000 IU/mg (International Units/mg, the International 65 2 GB2194886A 2 Unit being a unit of activity as defined by WHO, National Institute for Biological Standards and Control, Holly Hill, Hampstead, London, NW3 6RB, U.K.).

The amino acid sequence of t-PA preferably substantially corresponds to that set forth in Fig. 1. The sequence is thus identical to that in Fig. 1 or contains one or more amino acid deletions, substitutions, insertions, inversions or additions of allelic origin or otherwise, the resulting sequence having at least 80%, and preferably 90%, homology with the sequence in Fig. 1 and retaining essentially the same biological and immunological properties of the protein. In particular, the sequence is identical to that in Fig. 1 or has the same sequence but with the amino acid in the 245th position from the serine N-terminus being valine instead of methionine, either se- quence optionally having an additional polypeptide N-terminal presequence of Gly-Ala-Arg. 10 The amino acid sequence set forth in Fig. 1 has thirty-five cysteine residues and thus the potential for forming seventeen disulphide bridges. Based on analogy with other proteins whose structure has been determined in more detail, the postulated structure for the sequence (arising from disulphide bond formation) between the amino acid in the 90th position and the proline C terminus is set forth in Fig. 2. The structure of the N-terminal region is less certain although some proposals have been put forward (Progress in Fibrinolysis, 1983, 6, 269-273; and Proc.

Nad. Acad. Sci., 1984, 81, 5355-5359). The most important features of the structure of t-PA are the two kringle regions (between the 92nd and the 173rd amino acids and between the 180th and 261st amino acids), which are responsible for the binding of the protein to fibrin, and the serine protease region, which comprises the major part of the B-chain and which is respon- 20 sible for the activation of plasminogen. The amino acids of special significance in serine pro teases are the catalytic triad, His/Asp/Ser. In t-PA these occur at the 322nd, the 371st and the 463rd positions. The disulphide bridge between the 264th and 395th cysteine amino acid residues is also important in that it holds together the A- and the B- chains in the two-chain form of t-PA.

In Figs. 1 and 2, the conventional one and three letter codes have been employed for the amino acid residues as follows:

Asp D Aspratic acid Cys C Cystein Arg R Arginine 1 Thr T Threonine Val V Valine Lys K Lysine 30 Ser S Serine Ile I Isoleucine Trp W Tryptophan Clu E Glutamic acid Leu L Leucine Gln Q Glutamine Pro P Proline Tyr Y Tyrosine Met M Methionine 35 Gly G Glycine Phe F rhenylalanine Asn N Asparagine Ala A Alanine His H Histidine The t-PA may be obtained by any of the procedures described or known in the art. For example, it may be obtained from a normal or neoplastic cell line of the kind described in Biochimica et Biophysica Acta, 1979, 580, 140-153; EP-A-41 766 or EP-A-1 13 319. It is preferred, however, that t- PA is obtained from a cultured transformed or transfected cell line derived using recombinant DNA technology as described in, for example, EP- A-93 619; EP-A117 059; EP-A-117 060; EP-A-173 552; EP-A-174 835; EP-A-178 105; WO 86/01538; WO 86/05514; or WO 86/05807. It is particularly preferred that Chinese hamster ovary (CHO) cells are used for the production of t-PA and are derived in the manner as described in Molecular and Cellular Biology, 1985, 5(7), 1750-1759. In this way, the cloned gene is cotransfected with the gene encoding dihydrofolate reductase (dhfr) into dhfr- CHO cells. Transformants expressing dhfr 50 are selected on media lacking nucleosides and are exposed to increasing concentrations of methotrexate. The dhfr and t-PA genes are thus coamplified leading to a stable cell line capable of expressing high levels of t-PA.

The t-PA is, preferably, purified using any of the procedures described or known in the art, such as the procedures described in Biochimica et Biophysica Acta, 1979, 580, 140-153; J. 55 Bio/. Chem., 1979, 254(6), 1998-2003; ibid, 1981, 256(13), 7035-7041; Eur. J. Biochem., 1983, 132, 681-686; EP-A-41 766; EP-A-1 13 319; or GB-A-2 122 219.

t-PA may be used in the manner of the present invention either alone or in combination with another therapeutic agent which also inhibits damage to jeopardized tissue during reperfusion. A particularly useful example of such a combination is with superoxide dismutase (SOD), an enzyme 60 that is known to scavenge and destroy superoxide radicals, one of the phenomena capable of causing tissue damage. Indeed, it has also been found that the combination of t-PA and SOD provides a significantly potentiated level of inhibition compared with that provided by t-PA or SOD per se. Accordingly the present invention also provides a combination of t-PA and SOD.

The combination of t-PA and SOD affords a particularly convenient means both for the 65 3 GB2194886A 3 1 45 removal of blood clots and for the inhibition of damage to jeopardized tissue during subsequent reperfusion. Thus, the administration of t-PA and SOD will result first in the removal of the blood clot through the known thrombolytic action of t-PA and then in the inhibition of tissue damage through the combined action of t-PA and SOD.

The SOD of use in combination with t-PA may be any bioactive protein substantially corre- 5 sponding to any one or more of a group of enzymes known generally by this name. It is preferably of mammalian, and especially of bovine or human, origin and is generally associated with a metal cation by which it is normally classified. Examples of a metal cation include iron, manganese, copper and preferably combinations of copper with other metals, such as zinc, cadmium, cobalt or mercury, of which a copper/zinc combination is preferred. Both the mangan- 10 ese and the copper/zinc forms of SOD occur naturally in humans. The iron and manganese forms of SOD of bacterial origin both have a molecular weight of about 40,000 and are dimers. The manganese form of SOD of eukaryotic origin on the other hand has a molecular weight of about 80, 000 and is a tetramer. The copper/zinc form of SOD of eukaryotic origin has a molecular weight of about 32,000 and is a dimer with one copper cation and one zinc cation per subunit. The copper cation is ligated to four histidine residues per subunit and the zinc cation is ligated between histidine and aspartic acid. There is also a copper/zinc form of SOD of eukaryotic origin which has a molecular weight of about 130,000 and which consists of four subunits. The molecular weights of the various forms of SOD were estimated using sedimenta- tion equilibrium, molecular sieving or using polyacrylamide gels. The isoelectric points of the 20 various forms of SOD range from 4 to 6.5 depending on the degree of sulphation and/or deamidation. Preferably, the specific activity of the copper/zinc form of SOD of bovine or human origin is at least 3000 U/mg (the unit of activity being as defined in J. Biol. Chem., 1969, 244, 6049-6055).

The amino acid sequence of the copper/zinc form of SOD of bovine or human origin preferably 25 substantially corresponds to that set forth in J. Biol. Chem., 1974 249(22), 7326 to 7338, in the case of that of bovine origin, and Biochemistry, 1980, 19, 2310 to 2316 and FEBS Letters, 1980, 120, 53 to 55, in the case of that of human origin. The sequence is thus identical to that set forth in these articles or contains one or more amino acid deletions, substitutions, insertions, inversions or additions of allelic origin or otherwise, the resulting sequence having sufficient 30 homology with the published sequence so as to retain essentially the same biological and immunological properties.

The amino acid sequence of the copper/zinc form of SOD of bovine origin contains three cysteine residues per subunit (J. BioL Chem., 1974, 249(22), 7326-7338). The intrachain disulphide bridge occurs between the Cys 55 and Cys 144 residues while the interchain disul- 35 phide bridge occurs between the Cys 6 residues. The amino acid sequence of the copper/zinc form of SOD of human origin contains four cysteine residues per subunit (Biochemistry, 1980, 19, 2310 to 2316 and FEBS Letters, 1980, 120, 53 to 55). The intrachain disulphide bridge occurs between the Cys 57 and Cys 146 residues while the interchain disulphide bridge occurs also between the Cys 6 residues. The Cys 111 residue remains free.

The SOD may be obtained by any suitable procedure described or known in the art. For example, it may be obtained from erythrocytes or from liver by an extraction procedure of the kind described in GB-A-1 407 807 and GB-A-1 529 890. Alternatively, SOD may be obtained from a cultured transformed or transfected cell line, derived using recombinant DNA technology as described in, for example, Australian patent application 27461/84, WO 85/01503, EP-A-138 45 111, EP-A- 164 566, EP-A- 173 280 and EP-A- 180 964.

The SOD is preferably purified using any suitable procedure described or known in the art, such as the procedure described in EP-A-1 12 299.

In using t-PA, or a combination of t-PA and SOD, in the manner of the present invention, it is preferred to employ the active ingredient(s) in the form of a pharmaceutical formulation. In the 50 case of the combination, the active ingredients may be employed in separate formulations which may be administered simultaneously or sequentially. If they are administered sequentially, it is preferred to administer the t-PA formulation first and then the SOD formulation. In any event, the delay in administering the second of the two formulations should not be such or to lose the benefit of a potentiated effect of the combination of the active ingredients in vivo in inhibiting 55 tissue damage. However, rather than use separate formulations, it is much more convenient to present both active ingredients together in a single combined formulation. Accordingly, the present invention also provides a pharmaceutical formulation, which comprises t-PA and SOD and a pharmaceutically acceptable carrier.

Generally, t-PA, or the combination of t-PA and SOD, will be administered by the intravascular 60 route, whether by infusion or by bolus injection, and thus a parenteral formulation is required. It is preferred to present a Iyophilised formulation to the physician or veterinarian because of the significant transportation and storage advantages that it affords. The physician or veterinarian may then reconstitute the lyophilised formation in an appropriate amount of solvent as and when required.

4 GB2194886A 4 Parenteral and lyophilised pharmaceutical formulations containing t-PA are known in the art.

Examples of such art include EP-A-41 766; EP-A-93 619; EP-A-1 12 122; EPA-1 13 319; EP-A 123 304; EP-A-143 081; EP-A-156 169; WO 86/01104; Japanese patent publication 57 120523 (application 56-6936) and Japanese patent publication 58-65218 (application 56 163145). Additional preferred examples include GB-A-2 176 702 and GB-A-2 176 703. All such 5 formulations are also suitable for SOD and for the combination of t-PA and SOD.

Intravascular infusions are normally carried out with the parenteral solution contained within an infusion bag or bottle or within an electrically operated infusion syringe. The solution may be delivered from the infusion bag or bottle to the patient by gravity feed or by the use of an infusion pump. The use of gravity feed infusion systems does not afford sufficient control over the rate of administration of the parenteral solution and, therefore, the use of an infusion pump is preferred especially with solutions containing relatively high concentrations of active ingredi ents. More preferred, however, is the use of an electrically operated infusion syringe which offers even greater control over the rate of administration.

An effective amount of t-PA, and of a combination of t-PA and SOD, to inhibit damage to jeopardized tissue during reperfusion will of course depend upon a number of factors including, for example, the age and weight of the mammal, the precise condition requiring treatment and its severity, the route of administration, and will ultimately be at the discretion of the attendant physician or veterinarian. An effective dose, however, in the case of t- PA is generally in the range from 0.2 to 4 mg/kg (i.e. 100,000 to 2,000,000 IU/kg assuming a specific activity for t PA of 500,000 IU/mg), preferably from 0.3 to 2 mg/kg (i.e. 150,000 to 1, 000,000 IU/kg), bodyweight of patient per hour. Thus for a 70kg adult human being, an effective amount per hour will preferably be from 20 to - 140 mg (ie. 10,000,000 to 70,000,000 IU), especially about mg (i.e. 35,000,000). If SOD is used in combination with t-PA, then an effective dose of SOD is generally in the range of from 1000 to 50,000 U/kg, preferably from 7000 to 20,000 U/kg bodyweight of patient per hour. Thus for a 70 kg adult human being, an effective amount of S6D per hour will preferably be from 500,000 to 1,500,000 U.

The following examples are provided in illustration of the present invention and should not be construed in any way as constituting a limitation thereof.

Example 1: Preparation of Parenteral Formulation of t-PA A parenteral formulation of t-PA was prepared substantially as described in GB-A-2 176 703. The t-PA had a specific activity of about 300,000 IU/mg.

Example 2: Preparation of Parenteral Formulation of SOD Bovine SOD of the copper/zinc form was obtained from Sigma Chemical Co., St Louis, Missouri, U.S.A., 63178, as a powder and dissolved in substantially neutral physiological saline solution.

Example 3: Preparation of Parenteral Formulation of t-PA and SOD The formulations of Examples 1 and 2 were mixed and further diluted in physiological saline solution to achieve the required dosage.

Example 4: Protection of Jeopardized Tissue by t-PA and by t-PA and SOD (a) Methodology Male beagle dogs (10-12kg) were anaesthetized with pentobarbital sodium, intubated, and ventilated with room air via a Harvard respirator. Catheters for infusion and arterial blood pressure measurements were implanted in the left jugular vein and left carotid artery. A thoraco tomy was performed at the fourth intercostal space, the heart suspended in a pericardial cradle and the left anterior descending artery (LAD) isolated just below the first major diagonal branch. 50 An electromagnetic flow probe was placed on the LAD. A 90 minute occlusion of the LAD was produced by placing a snare of 1/0 silk sutre distal to the flow probe. Treatment was initiated intravenously fifteen minutes prior to release of this snare occlusion and continued for 45 minutes after release. The thoracotomy was closed, and the animals were allowed to recover from the surgical procedures. The animals were reanaesthetized 24 hours after the occlusion, and the flow in the LAD reassessed. Then the heart was removed for post mortern quantifica tion of infarct size.

Four groups of dogs were evaluated. Group 1 consisted of saline controls. Group 11 were administered 2.5 mg/kg (750,000 IU/kg) t-PA, Group III were administered 16,500 U/kg bovine SOD, and Group IV were administered both 2.5 mg/kg (750,000 IU/kg) t-PA and 16,500 U/kg 60 bovine SOD. The formulations used were as described in Examples 1 to 3.

Myocardial infarct size was quantified by an ex vivo dual reperfusion technique. Cannulas were inserted into the LAD immediately distal to the site of occlusion and into the aorta above the coronary ostia. The LAD coronary bed that was perfused with 1.5% triphenyl tetrazolium hydrochloride (TTC) in 0.02 M potassium posphate buffer, pH 7.4. The aorta was perfused in a 65 GB2194886A retrograde manner with 0.5% Evans blue dye. Both regions were perfused with their respective stains at a constant pressure of 100 mm mercury for five minutes. The heart was cut into 8 mm slices perpendicular to the apex-base axis. The area of the left ventricle at risk of infarction due to anatomical dependence on the LAD for blood flow is identified by the lack of Evans blue in this region. The region of infarcted myocardium within the area at risk was demarcated by the lack of staining of the tissue when perfused with TTC due to a loss of dehydrogenase enzymes.

The transverse ventricular sections were traced carefully on to clear acrylic overlays to provide a permanent record of infarct morphology and to allow planimetric confirmation of infarct size. Ventricular sections then were trimmed of right ventricular muscle, valvular, and fatty tissue. The 10 total left ventricle area at risk and infarct was separated by careful dissection and weighed. Infarct size was expressed as a percentage of the anatomic area at risk. Statistical comparison of the drug treatment group to the control group was made using a one way analysis of variance (anova) using Bonferroni's method for multiple comparison (Circulation Research, 1980, 47, 1-9). A p value of less than 0.05 was taken as the criterion of significance.

(b) Results TABLE

GROUP NUMBER AREA AT RISK % VENTRICLE OF DOGS INFARCTED AT RISK I. Saline 5 36.0 8.9 37.3 7.6 Ii. t-PA 6 14.3 + 11.7 35.7 + 5.4 111- SOD 4 13.0 + 4.6 30.6 + 2.6 35 IV. t-PA+ 3 2.3 + 1.3 37.2 + 9.1 SOD 40 Data are expressed as means + standard errors.

The proportion of the left ventricle made ischaemic by mechanical occlusion of the LAD was 50 not significantly different between any of the treatment groups and the control group by ANOVA.

so (c) Conclusions

The use of t-PA significantly inhibited the myocardial infarct size thus demonstrating its ability to protect jeopardized tissue during reperfusion. In addition, the combined use of t-PA and SOD 55 achieved a synergistic inhibitory effect in this regard, with the combination providing a level of inhibition greater than that provided by each of t-PA or SOD on its own.

Claims (17)

1. Use of t-PA for the manufacture of a medicament for the inhibition of damage to jeopar- 60 dized tissue during reperfusion in a mammal.

2. Use of t-PA and SOD for the manufacture of a medicament for the inhibition of damage to jeopardized tissue during reperfusion in a mammal.

3. Use of t-PA and SOD for the manufacture of a medicament for the removal of a blood clot and for the inhibition of damage to jeopardized tissue during reperfusion in a mammal. 65 6 GB2194886A 6

4. Use according to any one of the preceding claims, wherein the tissue is myocardial tissue.

5. Use according to any one of the preceding claims, wherein the t-PA is in the one-chain form.

6. Use according to any one of claims 1 to 4, wherein the t-PA is in the two-chain form.

7. Use according to claim 5 or claim 6, wherein the t-PA has the amino acid sequence set 5 forth in Fig. 1 or has the same amino acid sequence but with the amino acid in the 245th position from the serine N-terminus being valine instead of methionine, either sequence oplionally having an additional polypeptide N-terminal presequence of Gly-Ala-Arg.

8. Use according to claim 2 or claim 3, wherein the SOD is the copper/zinc form of bovine or human origin.

9. A combination of t-PA and SOD.

10. A combination of t-PA and SOD for use in human and veterinary medicine.

11. A combination of t-PA and SOD for use in the inhibition of damage to jeopardized tissue during reperfusion in a mammal.

12. A combination of t-PA and SOD for use in the removal of a blood clot and in the 15 inhibition of damage to jeopardized tissue during reperfusion in a mammal.

13. A combination according to any one of claims 9 to 12, wherein the tPA is in the onechain form.

14. A combination according to any one of claims 9 to 12, wherein the tPA is in the two- chain form.

15. A combination according to claim 13 or claim 14, wherein the t-PA has the amino acid sequence set forth in Fig. 1 or has the same amino acid sequence but with the amino acid in the 245th position from the serine N-terminus being valine instad of methionine, either sequence optionally having an additional polypeptide N-terminal presequence of Gly- Ala-Arg.

16. A combination according to any one of claims 9 to 12, wherein the SOD is the copper/zinc form of bovine or human origin.

17. A pharmaceutical formulation, which comprises a combination according to any one of claims 9 to 16 and a pharmaceutically acceptable carrier.

Published 1988 at The Patent Office, State House, 66/71 High Holborn, London WC 1 R 4TP. Further copies may be obtained from The Patent Office, Sales Branch, St Mary Cray, Orpington, Kent BR5 3RD. Printed by Burgess & Son (Abingdon) Ltd. Con. 1/87.

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