GB2189598A - Embedding apparatus for preparing microscopic samples - Google Patents

Embedding apparatus for preparing microscopic samples

Info

Publication number
GB2189598A
GB2189598A GB08708162A GB8708162A GB2189598A GB 2189598 A GB2189598 A GB 2189598A GB 08708162 A GB08708162 A GB 08708162A GB 8708162 A GB8708162 A GB 8708162A GB 2189598 A GB2189598 A GB 2189598A
Authority
GB
United Kingdom
Prior art keywords
dna
positions
organisms
genomic dna
dna fragments
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB08708162A
Other versions
GB8708162D0 (en
GB2189598B (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sakura Seiki Co Ltd
Chiyoda Manufacturing Corp
Original Assignee
Sakura Seiki Co Ltd
Chiyoda Manufacturing Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sakura Seiki Co Ltd, Chiyoda Manufacturing Corp filed Critical Sakura Seiki Co Ltd
Publication of GB8708162D0 publication Critical patent/GB8708162D0/en
Publication of GB2189598A publication Critical patent/GB2189598A/en
Application granted granted Critical
Publication of GB2189598B publication Critical patent/GB2189598B/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Abstract

A method of determining whether two organisms are identical comprises subjecting a restriction endonuclease digest of genomic DNA of the first organism to electrophoresis; determining the positions of the DNA fragments thus-separated which hybridise with one or more labelled DNA probes; and comparing these positions with the positions of DNA fragments binding to the or each probe and produced from genomic DNA of the second organism in like manner; an amount of probe DNA and one or more restriction endonucleases being used such that sufficient bands are revealed by the probing to achieve a sufficiently low probability (X) that the two organismswill have failed to have been distinguished when they appear identical from their band patterns, as determined by: X = F<q> wherein F is a fraction representative of the proportion DNA fragments which are identical between restriction endonuclease digests of genomic DNA of pairs of independently-obtained organisms of the same species as the first and second organisms and q is the number of positions revealed by the probing.
GB08708162A 1984-10-04 1985-10-04 Determination of identity between two organisms Expired GB2189598B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB848425059A GB8425059D0 (en) 1984-10-04 1984-10-04 Identification technique
PCT/GB1985/000450 WO1986002101A1 (en) 1984-10-04 1985-10-04 Determination of identity between two organisms

Publications (3)

Publication Number Publication Date
GB8708162D0 GB8708162D0 (en) 1987-05-13
GB2189598A true GB2189598A (en) 1987-10-28
GB2189598B GB2189598B (en) 1988-11-02

Family

ID=10567683

Family Applications (2)

Application Number Title Priority Date Filing Date
GB848425059A Pending GB8425059D0 (en) 1984-10-04 1984-10-04 Identification technique
GB08708162A Expired GB2189598B (en) 1984-10-04 1985-10-04 Determination of identity between two organisms

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GB848425059A Pending GB8425059D0 (en) 1984-10-04 1984-10-04 Identification technique

Country Status (4)

Country Link
EP (1) EP0227678A1 (en)
JP (1) JPS62500423A (en)
GB (2) GB8425059D0 (en)
WO (1) WO1986002101A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0528656A1 (en) * 1991-08-12 1993-02-24 Strix Limited Liquid heating vessels

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62111699A (en) * 1985-08-05 1987-05-22 コラボラテイブ・リサ−チ・インコ−ポレ−テツド Determination of gene form due to pleomorphism of limit fragment length
GB8608269D0 (en) * 1986-04-04 1986-05-08 Brown J J Ice-maker
GB8608629D0 (en) * 1986-04-09 1986-05-14 Biotechnica Ltd Labelling
EP0402400B1 (en) * 1988-02-18 1999-09-08 University of Utah Genetic identification employing dna probes of variable number tandem repeat loci
AU7777091A (en) * 1990-04-30 1991-11-27 University Of British Columbia, The Method of establishing identity
AUPP421798A0 (en) * 1998-06-18 1998-07-09 Kazamias, Christian Process of identification

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2135774A (en) * 1983-02-28 1984-09-05 Actagen Inc Identification of individual members of a species

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1984000979A1 (en) * 1982-08-24 1984-03-15 Wistar Inst Process for determining whether a herpersvirus is in the virulent or latent state
JP2539189B2 (en) * 1983-03-21 1996-10-02 ウエブスター，ジヨン エー．，ジユニヤ Method for identifying and characterizing organisms
EP0146589A4 (en) * 1983-05-26 1987-04-29 Plant Resources Inst Process for genetic mapping and cross-breeding thereon for plants.

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2135774A (en) * 1983-02-28 1984-09-05 Actagen Inc Identification of individual members of a species

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CHEMICAL ABSTRACTS, VOLUME 87, NR. 4, 25 JULY 1977, COLUMBUS, OHIO, (US)PAGE 191 *
CHEMICAL ABSTRACTS, VOLUME 92, NR. 1, 7 JANUARY 1980, COLUMBUS, OHIO, (US)PAGE 202 *
CHEMICAL ABSTRACTS, VOLUME 99, NR. 15, 10 OCTOBER 1983, COLUMBUS, OHIO, (US)PAGE 265 *
WO A 84/00979 *
WO A 84/04758 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0528656A1 (en) * 1991-08-12 1993-02-24 Strix Limited Liquid heating vessels

Also Published As

Publication number Publication date
EP0227678A1 (en) 1987-07-08
GB8708162D0 (en) 1987-05-13
WO1986002101A1 (en) 1986-04-10
JPS62500423A (en) 1987-02-26
GB2189598B (en) 1988-11-02
GB8425059D0 (en) 1984-11-07

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee