GB2157696A - Recombinant dna derived antigens of treponema pallidum - Google Patents
Recombinant dna derived antigens of treponema pallidumInfo
- Publication number
- GB2157696A GB2157696A GB08412158A GB8412158A GB2157696A GB 2157696 A GB2157696 A GB 2157696A GB 08412158 A GB08412158 A GB 08412158A GB 8412158 A GB8412158 A GB 8412158A GB 2157696 A GB2157696 A GB 2157696A
- Authority
- GB
- United Kingdom
- Prior art keywords
- antigens
- treponema pallidum
- treponemal
- clones
- recombinant dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 239000000427 antigen Substances 0.000 title abstract 7
- 102000036639 antigens Human genes 0.000 title abstract 7
- 108091007433 antigens Proteins 0.000 title abstract 7
- 241000589884 Treponema pallidum Species 0.000 title abstract 3
- 108020004511 Recombinant DNA Proteins 0.000 title 1
- 241000588724 Escherichia coli Species 0.000 abstract 2
- 230000000644 propagated effect Effects 0.000 abstract 2
- 208000006379 syphilis Diseases 0.000 abstract 2
- 108091005804 Peptidases Proteins 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 1
- 239000013602 bacteriophage vector Substances 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 208000015181 infectious disease Diseases 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
- 229920001184 polypeptide Polymers 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 241001515965 unidentified phage Species 0.000 abstract 1
- 229960005486 vaccine Drugs 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/571—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses for venereal disease, e.g. syphilis, gonorrhoea
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/20—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Spirochaetales (O), e.g. Treponema, Leptospira
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Hematology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Urology & Nephrology (AREA)
- Biophysics (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Virology (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
DNA fragments from Treponema pallidum are packaged into suitable bacteriophage vectors for propagation within E. Coli. The resulting plaques are screened for antigens reactive with antibodies to Treponema pallidum. Clones from positive plaques are further propagated and purified to provide antigens useful in the serodiagnosis and study of syphilis and as vaccines against syphilis infection. Plasmid subclones of treponemal antigen producing phage clones are propagated in E. coli to provide an alternate source of treponemal antigens. In addition to bacteriophage clones which encode for polypeptide treponemal antigens, a clone is also disclosed which encodes for a protease resistent heat labile treponemal antigen.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US44139182A | 1982-11-12 | 1982-11-12 | |
PCT/US1983/001718 WO1984001961A1 (en) | 1982-11-12 | 1983-11-04 | Recombinant dna derived antigens of treponema pallidum |
Publications (2)
Publication Number | Publication Date |
---|---|
GB8412158D0 GB8412158D0 (en) | 1984-06-20 |
GB2157696A true GB2157696A (en) | 1985-10-30 |
Family
ID=23752699
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB08412158A Withdrawn GB2157696A (en) | 1982-11-12 | 1983-11-04 | Recombinant dna derived antigens of treponema pallidum |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP0126765A1 (en) |
JP (1) | JPS59502131A (en) |
KR (1) | KR840006677A (en) |
GB (1) | GB2157696A (en) |
WO (1) | WO1984001961A1 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4868118A (en) * | 1986-09-30 | 1989-09-19 | Board Of Regents, The University Of Texas System | Cloning and expression of the 47-kilodalton antigen of treponema pallidum |
CA2193870C (en) | 1995-12-25 | 2000-07-18 | Nobuyuki Ise | Treponema pallidum fused antigen and assay for anti-treponema pallidum antibodies using the same fused antigen |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4288426A (en) * | 1980-03-20 | 1981-09-08 | Research Corporation | Serological test for syphilis |
US4294817A (en) * | 1977-11-25 | 1981-10-13 | International Diagnostic Technology, Inc. | Method of fluoro immunoassay |
-
1983
- 1983-11-04 JP JP84500174A patent/JPS59502131A/en active Pending
- 1983-11-04 EP EP84900155A patent/EP0126765A1/en not_active Withdrawn
- 1983-11-04 WO PCT/US1983/001718 patent/WO1984001961A1/en not_active Application Discontinuation
- 1983-11-04 GB GB08412158A patent/GB2157696A/en not_active Withdrawn
- 1983-11-12 KR KR1019830005374A patent/KR840006677A/en not_active Application Discontinuation
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4294817A (en) * | 1977-11-25 | 1981-10-13 | International Diagnostic Technology, Inc. | Method of fluoro immunoassay |
US4288426A (en) * | 1980-03-20 | 1981-09-08 | Research Corporation | Serological test for syphilis |
Non-Patent Citations (5)
Title |
---|
GENE, VOL, 12, PP. 301-309, 1980. * |
INFECTION AND IMMUNITY VOL. 23, PP. 392-397, FEBRUARY 1979. * |
INFECTION AND IMMUNITY VOL. 36, PP. 1238-1241, JUNE 1982. * |
SCIENCE VOL. 202, PP. 1279-1284, 22 DECEMBE * |
SCIENCE VOL. 216 PP. 522-523, 30 APRIL 1982. * |
Also Published As
Publication number | Publication date |
---|---|
WO1984001961A1 (en) | 1984-05-24 |
EP0126765A1 (en) | 1984-12-05 |
KR840006677A (en) | 1984-12-01 |
JPS59502131A (en) | 1984-12-27 |
GB8412158D0 (en) | 1984-06-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
HUP0001930A2 (en) | Modified tnfalpha molecules, dna encoding such modified tnfalpha molecules and vaccines comprising such modified tnfalpha molecules and dna | |
EP0752876A4 (en) | Monoclonal anbibodies which bind to tumor rejection antigen precursor mage-1, recombinant mage-1, and mage-1 derived immunogenic peptides | |
NO308413B1 (en) | Antigen mixture, DNA segment encoding an antigen, recombinant vector and host cell including the DNA segment and uses of an antigen | |
NZ235260A (en) | Borrelia burgdorferi antigens, monoclonal antibodies to these, pathogenic strain, dna encoding antigens, vectors and vaccines against lyme disease | |
EA199800973A1 (en) | POLYPEPTID BELKA-106 OF THE EXTERNAL MORAXELLA CATARRHALIS MEMBRANE, SEQUENCE OF THE GENE AND THEIR APPLICATION | |
EP0939653A4 (en) | Humanization of an anti-carcinoembryonic antigen anti-idiotype antibody and use as a tumor vaccine and for targeting applications | |
WO1995029193A3 (en) | Melanoma antigens | |
NO913228L (en) | ANTIBODIES AND PROCEDURES FOR THEIR PREPARATION. | |
NO924780L (en) | EXPRESSION OF SPECIFIC IMMUNOGENS USING VIRUS ANTIGEN | |
HUP9901546A2 (en) | Helicobacter pylori antigens and vaccine compositions | |
KR100215606B1 (en) | Toxoplasma gondii antigens, the preparation thereof and the diagnostic agent containing them | |
CA2168459A1 (en) | Fusion proteins containing the c-terminal of tetanus toxin linked to a heterologous protein | |
GB2145092B (en) | Protective peptide antigen | |
ATE131873T1 (en) | CYTOKINE | |
MY105080A (en) | Heat-labile toxin b subunit fusion proteins. | |
ATE115412T1 (en) | ANTI-IDIOTYPE ANTIBODIES AGAINST ANTI-HUMAN HIGH MOLECULAR ANTIGENS ASSOCIATED WITH MELANOMA. | |
GB2157696A (en) | Recombinant dna derived antigens of treponema pallidum | |
DK160888A (en) | Sound-insulating mineral wool body and method of producing it | |
SG95574A1 (en) | Immunoreactive antigens of hepatitia e virus | |
CA2075366A1 (en) | Immunologically active peptides or polypeptides from the parvovirus b19 | |
AU2911289A (en) | Carcinoma-associated antigens, and antibodies which recognize these antigens | |
EP0140689A3 (en) | Hybrid cell lines producing monoclonal antibodies directed against treponema and use thereof for immuno-diagnosis of treponemal infections | |
GB9002512D0 (en) | Polypeptides and dna encoding same | |
DE69322960D1 (en) | CONCEPTIBLE VACCINE | |
ZA896942B (en) | Allelic variants of plasmodium falciparum merozoite surface antigen |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
WAP | Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1) |