GB2157696A - Recombinant dna derived antigens of treponema pallidum - Google Patents

Recombinant dna derived antigens of treponema pallidum

Info

Publication number
GB2157696A
GB2157696A GB08412158A GB8412158A GB2157696A GB 2157696 A GB2157696 A GB 2157696A GB 08412158 A GB08412158 A GB 08412158A GB 8412158 A GB8412158 A GB 8412158A GB 2157696 A GB2157696 A GB 2157696A
Authority
GB
United Kingdom
Prior art keywords
antigens
treponema pallidum
treponemal
clones
recombinant dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB08412158A
Other versions
GB8412158D0 (en
Inventor
Michael A Lovett
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California
Original Assignee
University of California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California filed Critical University of California
Publication of GB8412158D0 publication Critical patent/GB8412158D0/en
Publication of GB2157696A publication Critical patent/GB2157696A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/571—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses for venereal disease, e.g. syphilis, gonorrhoea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/20—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Spirochaetales (O), e.g. Treponema, Leptospira
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

DNA fragments from Treponema pallidum are packaged into suitable bacteriophage vectors for propagation within E. Coli. The resulting plaques are screened for antigens reactive with antibodies to Treponema pallidum. Clones from positive plaques are further propagated and purified to provide antigens useful in the serodiagnosis and study of syphilis and as vaccines against syphilis infection. Plasmid subclones of treponemal antigen producing phage clones are propagated in E. coli to provide an alternate source of treponemal antigens. In addition to bacteriophage clones which encode for polypeptide treponemal antigens, a clone is also disclosed which encodes for a protease resistent heat labile treponemal antigen.
GB08412158A 1982-11-12 1983-11-04 Recombinant dna derived antigens of treponema pallidum Withdrawn GB2157696A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US44139182A 1982-11-12 1982-11-12
PCT/US1983/001718 WO1984001961A1 (en) 1982-11-12 1983-11-04 Recombinant dna derived antigens of treponema pallidum

Publications (2)

Publication Number Publication Date
GB8412158D0 GB8412158D0 (en) 1984-06-20
GB2157696A true GB2157696A (en) 1985-10-30

Family

ID=23752699

Family Applications (1)

Application Number Title Priority Date Filing Date
GB08412158A Withdrawn GB2157696A (en) 1982-11-12 1983-11-04 Recombinant dna derived antigens of treponema pallidum

Country Status (5)

Country Link
EP (1) EP0126765A1 (en)
JP (1) JPS59502131A (en)
KR (1) KR840006677A (en)
GB (1) GB2157696A (en)
WO (1) WO1984001961A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4868118A (en) * 1986-09-30 1989-09-19 Board Of Regents, The University Of Texas System Cloning and expression of the 47-kilodalton antigen of treponema pallidum
CA2193870C (en) 1995-12-25 2000-07-18 Nobuyuki Ise Treponema pallidum fused antigen and assay for anti-treponema pallidum antibodies using the same fused antigen

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4288426A (en) * 1980-03-20 1981-09-08 Research Corporation Serological test for syphilis
US4294817A (en) * 1977-11-25 1981-10-13 International Diagnostic Technology, Inc. Method of fluoro immunoassay

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4294817A (en) * 1977-11-25 1981-10-13 International Diagnostic Technology, Inc. Method of fluoro immunoassay
US4288426A (en) * 1980-03-20 1981-09-08 Research Corporation Serological test for syphilis

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
GENE, VOL, 12, PP. 301-309, 1980. *
INFECTION AND IMMUNITY VOL. 23, PP. 392-397, FEBRUARY 1979. *
INFECTION AND IMMUNITY VOL. 36, PP. 1238-1241, JUNE 1982. *
SCIENCE VOL. 202, PP. 1279-1284, 22 DECEMBE *
SCIENCE VOL. 216 PP. 522-523, 30 APRIL 1982. *

Also Published As

Publication number Publication date
WO1984001961A1 (en) 1984-05-24
EP0126765A1 (en) 1984-12-05
JPS59502131A (en) 1984-12-27
GB8412158D0 (en) 1984-06-20
KR840006677A (en) 1984-12-01

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Legal Events

Date Code Title Description
WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)