GB2096012A - Liquid composition programming device, especially for liquid chromatography - Google Patents

Liquid composition programming device, especially for liquid chromatography Download PDF

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Publication number
GB2096012A
GB2096012A GB8209325A GB8209325A GB2096012A GB 2096012 A GB2096012 A GB 2096012A GB 8209325 A GB8209325 A GB 8209325A GB 8209325 A GB8209325 A GB 8209325A GB 2096012 A GB2096012 A GB 2096012A
Authority
GB
United Kingdom
Prior art keywords
liquid
mixing chamber
mixer
programming device
chamber
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB8209325A
Other versions
GB2096012B (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Politechnika Gdanska
Original Assignee
Politechnika Gdanska
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Politechnika Gdanska filed Critical Politechnika Gdanska
Publication of GB2096012A publication Critical patent/GB2096012A/en
Application granted granted Critical
Publication of GB2096012B publication Critical patent/GB2096012B/en
Expired legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B01F35/717—Feed mechanisms characterised by the means for feeding the components to the mixer
    • B01F35/71805—Feed mechanisms characterised by the means for feeding the components to the mixer using valves, gates, orifices or openings
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
    • B01D15/166—Fluid composition conditioning, e.g. gradient
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/80—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis
    • B01F27/94—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis with rotary cylinders or cones
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B01F35/712—Feed mechanisms for feeding fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B01F35/717—Feed mechanisms characterised by the means for feeding the components to the mixer
    • B01F35/7176—Feed mechanisms characterised by the means for feeding the components to the mixer using pumps
    • B01F35/717613—Piston pumps
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/24—Automatic injection systems
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/40—Mixing liquids with liquids; Emulsifying
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
    • G01N2030/347—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient mixers

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Accessories For Mixers (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

A liquid composition programming device, especially for liquid chromatography, comprising a mixing chamber and electromagnetic valves connected with liquid tanks, wherein in the mixer (1) in the immediate vicinity of liquid inlets (3) to the mixing chamber (2), seats (9) of at least two electromagnetic valves (10) are fixedly mounted, the closing members (11) of which have at their end portions the form of an inverted frustum of a cone, with the wider portion of the cone situated between the wall of the chamber (2) and the seat (9) of the electromagnetic valve (10). The mixer (1) has the form of a cylinder, and the liquid inlets (3) to the mixing chamber (2) are disposed on the periphery thereof. Liquid is fed via (5) to a chromatographic column, not shown; (6) is a rotary stirrer. <IMAGE>

Description

SPECIFICATION Liquid composition programming device, especially for liquid chromatography This invention relates to a liquid composition programming device, especially for liquid chromatography.
A liquid composition programming system known from the US Patent Specification No. 4,004,884 is provided with a connector, which is connected by n electronically controlled two-way electromagnetic valves to n tanks of various liquids, connected to a common source of compressed gas.
During the liquid composition programming, at any given moment, only one two-way valve is opened for a period proportional to the assumed participation of liquid, supplied by it to the mixing chamber.
A A disadvantage of the system described above is the presence of all n liquids in the mixing chamber, wherein the separate liquid streams mix into one stream, which therefore causes an unavoidable contamination of the liquids being programmed with the remaining liquids which should not participate in the process. In chromatography, this can cause a deactivation of the column or an appearance of additonal peaks on the sorptogram and a base line drift.
Another disadvantage of the system is gassing of the individual liquids in the tanks with the gas being under pressure. Since in chromatography, generally high pressures of the order of 50 - 500 atm. are used, the liquid within the tank undergoes gassing for several minutes. The gas dissolved in the liquid causes a disadvantageous variation of its properties, and makes the operation of the chromatograph impossible due to degassing of the liquid within the flow-through cuvette of the detector. Moreover, within the mixing chamber employed in the system, no mixing of the components occurs, thus the spectrum of the composition of liquids being programmed will at separate moments differ from the set one.
Furthermore the location of the two-way valves and the liquid tanks in the pressurized portion of the system makes it necessary that said elements must be resistant to high pressures.
The known instrument for programming the liquid composition, manufactured by the American Comapny Spectra Physics, consists of a three-way valve connected from one side with three liquid tanks, and from the other side, through a mixer, with the suction line of a piston pump The mixer shows a dead capacity of 1.9 ml. The outlet of each of the lines supplying the liquid to the valve is provided with a membrane actuated by means of an electromagnetic coil. At the place of connection of three lines, each of which contains another liquid, there are present at each moment all the liquids irrespective of the amounts of liquids being programmed.
The substantial disadvantage of the above described system is the permanent contact of the liquids being actually programmed, which are contained tanks connected with the valve. This causes a contamination of the liquids being progammed with other liquids, which can lead to a deactivation of the chromatographic column, base line drifts and the like. Another disadvantage is the large dead capacity of the apparatus, determined by the value-mixerpump connection.
A liquid composition programming device, espe ciallyfor liquid chromatography, according to the invention, comprises a mixing chamber and electromagnetic valves, connected with the liquid tanks, wherein, in the mixer, in the immediate vicinity of the liquid inlets to the mixing chamber, the seats of at least two electromagnetic valves are fixedly mounted, the sealing elements of said valves having at their upper portion the form of an inverted frustum of a cone, with the wider portion of the cone being situated between the mixing chamber wall and the electromagnetic valve seat.
The mixer may have been cylidrical form with the liquid inlets to the chamber being disposed on the periphery thereof.
The electromagnetic valves are in fact two-way valves. The device can operate both on the pressure and the suction side of the pump. When it is situated on the pressure side of the pump, the liquid tanks have the form of a helically coiled conduit.
The profiling of the mixer and the mixing chamber into the form of a cylinder enables a maximum filling of the chamber with the stirrer, and thus a minimalization of the dead capacity of the chamber. Owing to this, it is possible to program exactly the composition of the movable phase even without stirring the liquids flowing into the chamber, at least within the concentration range of 10 through 90%.
At present there are continued studies on the repeatablity and accuracy of the gradient courses in dependence on the dead capacity of the mixing chamber, on the rate of the movable phase, and the mixing time of its components.
Advantage of the device include the elimination of the necessary stirring of the liquids which in the given process are not subject to a programming of the liquid composition from n components, while the concentration of the individual components on the function of time are optional. Moreover, the liquid tanks, the electromagnetic valves, and the mixer can be disposed both on the pressure and on the suction side of the pump. There exists also the possibility to match an adequate capacity of the chamber to the delivery of the pump and to the basic time of the liquid composition programming involving the liquid being fed onto the chromatographic column.The invention will now be explained by means of an exemplary embodiment with reference to the accompanying drawing wherein Figure 1 is the longitudinal sectional view of the liquid composition programming device according to the invention; and Figure 2 is the general block diagram of a liquid chromatograph including a liquid composition programming device according to the invention.
As shown in Figure 1, the device is provided with a mixer 1 having the form of a cylinder, and having a mixing chamber 2 on the periphery of which two liquid inlets 3 are disposed. The mixing chamber 2 is limited from the top with a piston 4 in the axis of symmetry of which the liquid discharge channel 5 is disposed, and which is provided with a mechanical agitator 6 having the form of a cylinder, with its driving shaft 7 being mounted in a stuffing-box 8.
The diameter of the agitator 6 is smaller than that of the chamber 2 by about imam. In the immediate vicinity of the liquid inlet 3 is situated the seat 9 of the electromagnetic two-way valve 10 the closing member 11 of which the upper portion is shaped into a form of an inverted frustum of a cone, the wider portion of which is situated above the seat 9 of the valve 10. The closing member 11 is rigidly connectedto a magnet 12 movable slidablywithin the body 13 of the valve 10, whereby in the inside wall of the body 13 there is disposed a liquid inlet channel 14 connected by means of a conduit 15 to the liquid tank 16, while on the body 13 an electrical coil 17 is drawn over. The stuffing-box 8 is provided with a gland 18 and a sealing ring 19.
The seat 9 of the valve 10 is sealed between the body 13 of the valve 10 and the mixer 1 by means of Teflon washers 20.
According to Figure 2, the mixer 1 is connected to the piston pump 21, and, by the electric coils 17 drawn over the body 13 of the electromagnetic valve 10, to the electronic control unit 22. The piston pump 21 pumps the liquid mixture through the feeder 23 and the chromaographic column 24 to the detector 25 which is connected to a recorder 26.
The operation of the device is that, at that at first, the piston pump 21 and the mechanical actuator 6 are started. The electric coils 17 of the valves 10 become connected to the control unit 22, and the time of operation of each valve is in this way programmed so that they open alternately. From the tank 16 the pump sucks the liquid, through the valve 10 opened at that moment and the inlet 3 flowing then into the mixing chamber 2 wherein it is mixed with portions of liquid already present in the chamber 2, and currently flowing through the opened valve 10 proportional to its opening time.
After obtaining a mixture of the separate liquids, having the preset quantative composition, it is supplied to the chromatographic column 24 by the pump 21 and the feeder 23. After passing through the chromatographic column 24 the liquid mixture passes through the detector 25 to the gutter.
The electric signal from the detector 25 is registered in the recorder 26.
The number of valves 10 with which the mixer 1 is provided determines the number of components of the liquid mixture being progammed.

Claims (4)

1. A liquid composition programming device, especially for the liquid chromatography, consisting of a mixing chamber and electromagnetic valves connected with liquid tanks, wherein, in the mixer the immediate vicinity of liquid inlets into the mixing chamber, the seats of at least two electromagnetic valves are fixedly mounted, the closing members of said valves having at their upper portions the form of an inverted frustum of a cone, with the wider portion of the cone being situated between the wall of the chamber and the seat of the electromagnetic valve.
2. A device as claimed in Claim 1, wherein the mixer has the form of a cylinder, and the liquid inlets into the mixing chamber are disposed on the periphery thereof.
3. Adevice as claimed in Claim 1, wherein the electromagnetic valves are two-way valves.
4. A liquid composition programming device, substantially as hereinbefore described with reference to and as illustrated in the accompanying drawings.
GB8209325A 1981-04-06 1982-03-30 Liquid composition programming device especially for liquid chromatography Expired GB2096012B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL1981230992A PL134765B1 (en) 1981-04-06 1981-04-06 Apparatus for programming compositions of liquids in particular for use in gas chromatography

Publications (2)

Publication Number Publication Date
GB2096012A true GB2096012A (en) 1982-10-13
GB2096012B GB2096012B (en) 1984-08-22

Family

ID=20008338

Family Applications (1)

Application Number Title Priority Date Filing Date
GB8209325A Expired GB2096012B (en) 1981-04-06 1982-03-30 Liquid composition programming device especially for liquid chromatography

Country Status (7)

Country Link
CS (1) CS233727B2 (en)
DD (1) DD202247A5 (en)
DE (1) DE3212604A1 (en)
FR (1) FR2503418A1 (en)
GB (1) GB2096012B (en)
HU (1) HU183880B (en)
PL (1) PL134765B1 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0121127A1 (en) * 1983-03-07 1984-10-10 International Business Machines Corporation Liquid chromatography proportioning valve and mixer
EP1231994A1 (en) * 1999-10-29 2002-08-21 Waters Investments Limited High pressure capillary liquid chromatography solvent delivery system
GB2428392B (en) * 2002-12-09 2007-09-26 Waters Investments Ltd Backflow prevention for high pressure gradient systems
WO2019186223A1 (en) * 2018-03-28 2019-10-03 Bio-Rad Laboratories, Inc. Fluid mixer, pressure sensor

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB329839A (en) * 1929-05-18 1930-05-29 Frank Peto Barber Improvements in mixing valves for liquids
FR1201127A (en) * 1958-08-05 1959-12-28 Majeste La Reine De Droit Du C Valve device for selective outlets of tilting and reversing tanks of aircraft
DE1493663C3 (en) * 1964-05-30 1975-01-09 Farbwerke Hoechst Ag, Vormals Meister Lucius & Bruening, 6000 Frankfurt Process and device for the production of alkali salts of aromatic hydroxy compounds
DE7325738U (en) * 1973-07-13 1973-12-20 Brand W DEVICE FOR THE PRODUCTION OF BEVERAGES, IN PARTICULAR TEA
US4004884A (en) * 1976-07-02 1977-01-25 Hoffmann-La Roche Inc. Time division metering system
DE2758297A1 (en) * 1977-12-27 1979-06-28 Desma Werke Gmbh Polyurethane foam mixing head - with rotary valves for mixing chamber or reflux system

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0121127A1 (en) * 1983-03-07 1984-10-10 International Business Machines Corporation Liquid chromatography proportioning valve and mixer
EP1231994A1 (en) * 1999-10-29 2002-08-21 Waters Investments Limited High pressure capillary liquid chromatography solvent delivery system
EP1231994A4 (en) * 1999-10-29 2008-03-12 Waters Investments Ltd High pressure capillary liquid chromatography solvent delivery system
GB2428392B (en) * 2002-12-09 2007-09-26 Waters Investments Ltd Backflow prevention for high pressure gradient systems
WO2019186223A1 (en) * 2018-03-28 2019-10-03 Bio-Rad Laboratories, Inc. Fluid mixer, pressure sensor

Also Published As

Publication number Publication date
PL134765B1 (en) 1985-09-30
PL230992A1 (en) 1982-10-11
GB2096012B (en) 1984-08-22
DE3212604A1 (en) 1982-10-14
DD202247A5 (en) 1983-09-07
HU183880B (en) 1984-06-28
FR2503418A1 (en) 1982-10-08
CS233727B2 (en) 1985-03-14

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Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee