GB2088740A - Control unit for the apparatus for the overpressured layer chromatography - Google Patents
Control unit for the apparatus for the overpressured layer chromatography Download PDFInfo
- Publication number
- GB2088740A GB2088740A GB8135704A GB8135704A GB2088740A GB 2088740 A GB2088740 A GB 2088740A GB 8135704 A GB8135704 A GB 8135704A GB 8135704 A GB8135704 A GB 8135704A GB 2088740 A GB2088740 A GB 2088740A
- Authority
- GB
- United Kingdom
- Prior art keywords
- control unit
- pressure
- cushion
- overpressured
- layer chromatography
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000004587 chromatography analysis Methods 0.000 title description 12
- 230000002093 peripheral effect Effects 0.000 claims description 4
- 239000003480 eluent Substances 0.000 abstract description 9
- 238000000429 assembly Methods 0.000 abstract 1
- 239000002904 solvent Substances 0.000 description 4
- 239000002594 sorbent Substances 0.000 description 4
- 238000011161 development Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 238000013375 chromatographic separation Methods 0.000 description 2
- 238000013508 migration Methods 0.000 description 2
- 230000005012 migration Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000011158 quantitative evaluation Methods 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 108010001267 Protein Subunits Proteins 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 239000011877 solvent mixture Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
- G01N2030/906—Plate chromatography, e.g. thin layer or paper chromatography pressurised fluid phase
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
An overpressured chromatographic apparatus consists of two sub-assemblies interconnected in a releasable manner, namely a pressurized cushion ultramicrochamber UM and a controller which includes a logic control unit VL, an eluent pump Pe, a cushion pump Pp, a thermostat Tt, a display unit Kj, keyboard (B1)-operated manipulators Ksz, a printer Ny and an interface J for a computer. Display units may indicate when operating temperature and pressure have been reached, in sufficient pressure, end of run, etc., and time of run, temperature, pressure of cushion and pressure of element may all be controlled. <IMAGE>
Description
SPECIFICATION
Control unit for the apparatus for the overpressured layer chromatography
The invention relates to a control unit for the apparatus for the overpressured layer chromatography connected in a releasable manner to a pressurized ultramicrochamber serving for automated performance of overpressured layer chromatographic development (run).
In a recently developed planar liquid-chromatographic technique, known as overpressured layer chromatography, the sorbent layer is completely covered by means of a cover plate subjected to external pressure, while the solvent is caused to flow in a forced stream by means of a pump system through the particles of the sorbent (Hungarian Patent Specification
No. 173,749). The so-called pressurized ultramicrochamber may be circular (J. Chromatog. 174, 74/1979) or linear (British Patent
Application No. 81.16364, Publication No.
2078127).
These two versions of the overpressured layer chromatography exhibit several advantageous features when compared with 'classical' layer chromatography, and with high-performance thin layer chromatography, as follows: -shorter running time, even for longer mig
ration distances (30-40 cm) and even on
fine-particle sorbent layers; better resolution; -the possibility of visual evaluation; -acceleration of the migration of viscous
solvent mixtures; -modelling of high-performance column
chromatographic circumstances.
The operational characteristics of linear and circular overpressured layer chromatographic apparatus are as follows:
a) Separation always takes place within a given distance and with a predetermined flow velocity of the solvent. These values always depend on the task set (e.g. separation of two or more components of different substance groups), on the layer thickness, on the size of the sorbent particles, etc.
b) The cushion pressure is to be kept at a constant value (with a damaged cushion this aim cannot be achieved).
c) The value of the input pressure produced by the pump feeding the solvent, varies in compliance with the related function; however, the cushion pressure involves a certain restriction within the system. A deviation from the function results in an irregular development.
d) In the pressurized ultramicrochambers developed up to now, the base plate can be thermostated, resulting in a well reproducible value. The deviation of the temperature value from the selected temperature programme does not enable reproduction, the sensing and correction thereof in due time is of utmost importance.
Accordingly, it can be stated that when manipulating the apparatus presently used, the operator has to monitor the closed state of the apparatus, the pressure of the cushion, the pressure and the quantity of the eluent, the course and the end of the run.
This manifold observation occupies the operator for the full time and distracts him from performing any other work (e.g. preparation of the samples, or quantitative evaluation), in addition to this, it may result in several subjective faults.
The aim of the invention is to eliminate or reduce the imperfections mentioned above and to develop a control unit for meeting practical requirements. Accordingly, apparatus for overpressured layer chromatography according to the invention is an assembly of instruments built up from two units.
One unit is the actual apparatus for the overpressured layer chromatography (the pressurized ultramicrochamber), the other is the control unit, while the peripheral apparatuses are either connected to the control unit or they are incorporated therein. The control unit may also contain two pumps, reservoir(s) for the eluent and the water, a thermostat and instruments for control, regulation, measuring, data collecting and storage.
The invention is based on the discovery that overpressured layer chromatographic separation may be performed in a more standardized, reproducible manner and with a higher operational safety, when the total operation is controlled, whereby this technique, namely overpressured layer chromatography, can be more readily included into the repertoire of up-to-date technical methods of separation.
The invention is based on the further discovery that by using the automatic control, the subjective errors accompanying known methods of overpressured layer chromatographic separation can be considerably reduced.
It has been also recognized that by the application of the control unit embodied in the invention the working hours of an operator may far better be utilized, e.g. in preparation of the samples, instrumental quantitative evaluation, etc.
Accordingly, the invention relates to a control unit for overpressured layer chromatographic apparatus, including as sub-units the manipulating organs, such as a mains power stitch adapted simultaneously to actuate a thermostat, a start-switch by means of which the programme of a run may be put into operation, the run being stoppable by the operator by actuating the stop-switch. In addition to these, the pressure of the cushion, the flow velocity and the operational pressure of the eluent, the duration of development (run) and the operational temperature can be adjusted.
Display units are also associated with the sub-units, so e.g. the heated state of the thermostat and reaching the value of operational temperature are signalled by a lamp.
When the pressure of the eluent reaches the critical value, a signal lamp is lit and the run is stopped or the output of the pump is reduced to the proper value. The apparatus gives a signal when it is switched on, when the quantity of liquid (expediently water) present is insufficient for pumping the cushion, when the quantity of eluent is insufficient, or when the temperature deviates from the prescribed value. The end of the run is accompanied by an acoustic and visual signal; the parameter-sensors and measuring devices measure the temperature, the flow rate, the pressure and the time elapsed.
A schematic arrangement of apparatus for overpressured layer chromatography according to the invention is shown in Fig. 1.
Fig. 1 shows an ultramicrochamber UM receiving as inputs the outputs of pump Pe delivering eluent and of pump Pp delivering a pressurized fluid, e.g. air, for establishing and maintaining a pressurized cushion in the ui- tramicrochamber bBM. A thermostat is designated by Tt. A central controlling logic unit VL is operatively connected to pumps r-e and Pp as well as to the thermostat Tt. Additional, control unit VL is interconnected with a display unit Kj, the manipulating organs K, a printer Ny and an interface J connectible to a computer or other processing unit, as indicated by the nght-hand arrow. Finally, a keyboard unit B1 is connected to the manipulating organs K, > .
The main phases of the operation of the apparatus for overpressured layer chromatography provided with the control unit are as follows: 1 ) After having pressed the mains switch the thermostat benins to function and indicates the working condition of tile apparatus;
2) the adjustment of the various parameters to the desired values takes place;
3) positioning of the ohromatographic plate and closing the apparatus manually are carried out; 4) after pressing the start-switch, the cushion is pumped (e.g. with water) to the preselected pressure value;
5) the flow of eluent is commenced with a preselected flow velocity;
6) after a predetermined time interval, i.e.
after having consumed a certain quantity of the solvent, the eluent flow is stopped;
7) the pressure of the cushion drops to zero;
8) the apparatus gives an acoustic and/or light signal, indicating that the apparatus may be opened; and
9) the chromatographic plate is removes' from the opened apparatus and evaluated.
Claims (6)
1. A control unit for overpressured layer chromatographic apparatus for assuring automatic operation of the apparatus, the control unit comprising a plurality of sub-units for the adjustment of manipulation, display and metering units adapted to measure the parameters.
2. The control unit claimed in claim 1 in combination with means enabling it to be connected to the ultramicrochamber of said apparatus in a releasable manner.
3. The control unit or the combination as claimed in claim 1 or 2 wherein the control unit and the peripheral units are arranged separately.
4. The control unit or the combination as claimed in claim 1 or claim 2 wherein the control unit and the single peripheral units are assembled together.
5. The control unit as claimed in claim 1 in combination with the peripheral units and a pressurized ultramicrochamber assembled into a single integrated unit.
6. A control unit as claimed in claim 1 or the combination claimed in any of claims 2 to 5, substantially as herein described with reference to and as shown in the accompanying drawing.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| HU802921A HU187282B (en) | 1980-12-05 | 1980-12-05 | Control unit for row-chromatographic equipment under high pressure |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB2088740A true GB2088740A (en) | 1982-06-16 |
Family
ID=10961685
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB8135704A Withdrawn GB2088740A (en) | 1980-12-05 | 1981-11-26 | Control unit for the apparatus for the overpressured layer chromatography |
Country Status (9)
| Country | Link |
|---|---|
| CH (1) | CH658727A5 (en) |
| DD (1) | DD202346A5 (en) |
| DE (1) | DE3147405A1 (en) |
| FR (1) | FR2495779B1 (en) |
| GB (1) | GB2088740A (en) |
| HU (1) | HU187282B (en) |
| IT (1) | IT8149823A0 (en) |
| PL (1) | PL234122A1 (en) |
| SE (1) | SE8106866L (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2803220A1 (en) * | 2000-01-04 | 2001-07-06 | Bionisis | DEVICE AND METHOD FOR PROCESSING A SAMPLE BY SEPARATING ON A STATIONARY PHASE, UNDER CONTROLLED FORCE FLUX |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2173125B (en) * | 1985-04-04 | 1989-04-19 | Mueszeripari Muevek Lab | Apparatus for overpressured thin-layer chromatographic technique |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3826905A (en) * | 1971-10-27 | 1974-07-30 | Suomen Sokeri Oy | Methods and apparatus for providing automatic control of chromatographic fractionating processes |
-
1980
- 1980-12-05 HU HU802921A patent/HU187282B/en not_active IP Right Cessation
-
1981
- 1981-11-16 CH CH7340/81A patent/CH658727A5/en not_active IP Right Cessation
- 1981-11-18 SE SE8106866A patent/SE8106866L/en not_active Application Discontinuation
- 1981-11-26 GB GB8135704A patent/GB2088740A/en not_active Withdrawn
- 1981-11-27 DD DD81235202A patent/DD202346A5/en unknown
- 1981-11-30 DE DE19813147405 patent/DE3147405A1/en not_active Withdrawn
- 1981-12-03 IT IT8149823A patent/IT8149823A0/en unknown
- 1981-12-04 FR FR8122734A patent/FR2495779B1/en not_active Expired
- 1981-12-07 PL PL23412281A patent/PL234122A1/xx unknown
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2803220A1 (en) * | 2000-01-04 | 2001-07-06 | Bionisis | DEVICE AND METHOD FOR PROCESSING A SAMPLE BY SEPARATING ON A STATIONARY PHASE, UNDER CONTROLLED FORCE FLUX |
| WO2001050123A1 (en) * | 2000-01-04 | 2001-07-12 | Bionisis S.A. | Device and method for treating a sample by overpressured layer chromatography a stationary phase, under controlled forced flow |
| AU770513B2 (en) * | 2000-01-04 | 2004-02-26 | Bionisis S.A. | Device and method for treating a sample by overpressured layer chromatography a stationary phase, under controlled forced flow |
Also Published As
| Publication number | Publication date |
|---|---|
| PL234122A1 (en) | 1982-08-02 |
| SE8106866L (en) | 1982-06-06 |
| CH658727A5 (en) | 1986-11-28 |
| DD202346A5 (en) | 1983-09-07 |
| FR2495779B1 (en) | 1985-12-13 |
| HU187282B (en) | 1985-12-28 |
| IT8149823A0 (en) | 1981-12-03 |
| FR2495779A1 (en) | 1982-06-11 |
| DE3147405A1 (en) | 1982-11-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| WAP | Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1) |