GB2078974A - Vessel for determining the electrophoretic movability of particles - Google Patents
Vessel for determining the electrophoretic movability of particles Download PDFInfo
- Publication number
- GB2078974A GB2078974A GB8120057A GB8120057A GB2078974A GB 2078974 A GB2078974 A GB 2078974A GB 8120057 A GB8120057 A GB 8120057A GB 8120057 A GB8120057 A GB 8120057A GB 2078974 A GB2078974 A GB 2078974A
- Authority
- GB
- United Kingdom
- Prior art keywords
- coating
- vessel
- methyl cellulose
- electrophoretic
- particles
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Electrochromic Elements, Electrophoresis, Or Variable Reflection Or Absorption Elements (AREA)
- Optical Measuring Cells (AREA)
Abstract
In a vessel for determining the electrophoretic movability of particles, the inner wall of the vessel is coated with a substituted triethoxysilane, preferably aminopropyltriethoxysilane and methyl cellulose, prepared by applying a coating of a substituted triethoxysilane 2 to the interior wall of the vessel 1, applying a coating of methyl cellulose 3 to the coating of substituted triethoxysilane, and repeating this sequence of coatings at least once; the same or a different substituted triethoxy silane being used in each such sequence. <IMAGE>
Description
SPECIFICATION
Vessel for determining the electrophoretic movability of particles
The invention relates to all vessels for electrophoretic apparata. The invention can be used in all cell electrophoresis methods such as cell electrophoresis, free-flow electrophoresis, in all detection methods such as the laser Doppler principle and in optomechanical simulations of the same, and in all analytic and preparative methods.
It is known to coat the interior walls of vessels with a 5% albumen solution (K. Hannig. et.al. Hoppe
Seyler's Journal for Physiological Chemistry 356 (1975) 1209). This solution has the disadvantage that only a slight improvement in the electroosmotic profile is obtained. It is also a disadvantage that this coating is not stable with respect to time and can become detached when the vessel is cleaned for example with 30% formic acid). It is known to coat the interior walls of the vessel with methyl cellulose.
However, this coating can only be applied to unused or freshly cleaned interior walls of vessels. Very flat electroosmotic profiles of the vessels are thereby obtained. However, the profile is less stable. Again this coating becomes detached when the vessel is cleaned (for example with diluted formic acid).
It is also known to coat the interior walls of the vessels with 1% aminopropyltriethoxysilane (K. Hannig et.al). Although this coating is more stable with respect to time, it does not result in electroosmotic profiles which are as flat as those which occur when using methyl cellulose.
The purpose of the invention is to provide a vessel for determining the electrophoretic movability of particles and which renders it possible to determine the electrophoretic movability of particles as accurately as possible with high productivity, a low concentration of the particles to be measured, and small sample volumes.
The object of the invention is to provide a vessel for determining the electrophoretic movability of particles having a flat electroosmotic profile which is stable with respect to time. In accordance with the invention, this object is achieved by a vessel, for determining the electrophoretic movability of particles, which is coated with aminopropyltriethoxysilane and/or methyl cellulose characterised in that a coating of substituted triethoxysilane such as cyclohexenylethyl- or glycidoxypropyl triethoxysilane is applied to the interior wall of the vessel, a coating of methyl cellulose is applied to the coating of substituted triethoxysilane, and that this sequence of coatings is repeated at least once, the same or a different substituted triethoxysilane being used in each such sequence.
The invention will be further described with reference to the drawing which shows, diagrammatically, the arrangement of the coatings on the vessel for determining the electrophoretic movability of particles.
A coating 2 of cyclohexenylethyltriethoxysilane is applied to the interior wall 1 of the vessel. A coating 3 of methyl cellulose is applied to the aforesaid coating.
A coating 4 of aminopropyltriethoxysilane is applied to the coating 3 of methyl cellulose. A coating 3 of methyl cellulose is applied to the coating 4 of aminopropyltriethoxysilane, and a coating 5 of glycidoxypropyltriethoxysilane is applied to the coating 3 of methyl cellulose. A coating 3 of methyl cellulose is applied to the coating 5 of glycidoxypropyltriethoxysilane.
Alternatively, it is possible to use parts of the stated sequence of coatings.
1. A vessel for determining the electrophoretic movability of particles, the inner wall of which vessel is coated with aminopropyltriethoxysilane and/or methyl cellulose, characterised in that a coating of a substituted triethoxysilane is applied to the interior wall of the vessel, a coating of methyl cellulose is applied to the coating of substituted triethoxysilane, and that this sequence of coatings is repeated at least once, the same or a different substituted triethoxy silane being used in each such sequence.
2. A vessel as claimed in claim 1, in which the substituted triethoxy silane used to form at least one of the coatings is cyclohexenylethyl triethoxysilane.
3. A vessel as claimed in claim 1 or 2, in which the substituted triethoxy silane used to form at least one of the coatings is glycidoxypropyl triethoxysilane.
4. A vessel as claimed in claim 1 substantially as hereinbefore described with reference to and as illustrated in the accompanying drawing.
**WARNING** end of DESC field may overlap start of CLMS **.
Claims (4)
1. A vessel for determining the electrophoretic movability of particles, the inner wall of which vessel is coated with aminopropyltriethoxysilane and/or methyl cellulose, characterised in that a coating of a substituted triethoxysilane is applied to the interior wall of the vessel, a coating of methyl cellulose is applied to the coating of substituted triethoxysilane, and that this sequence of coatings is repeated at least once, the same or a different substituted triethoxy silane being used in each such sequence.
2. A vessel as claimed in claim 1, in which the substituted triethoxy silane used to form at least one of the coatings is cyclohexenylethyl triethoxysilane.
3. A vessel as claimed in claim 1 or 2, in which the substituted triethoxy silane used to form at least one of the coatings is glycidoxypropyl triethoxysilane.
4. A vessel as claimed in claim 1 substantially as hereinbefore described with reference to and as illustrated in the accompanying drawing.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DD22229080A DD156337A3 (en) | 1980-07-01 | 1980-07-01 | KUEVETTE FOR DETERMINING THE ELECTROPHORETIC MOBILITY OF PARTICLES |
Publications (1)
Publication Number | Publication Date |
---|---|
GB2078974A true GB2078974A (en) | 1982-01-13 |
Family
ID=5525074
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB8120057A Withdrawn GB2078974A (en) | 1980-07-01 | 1981-06-30 | Vessel for determining the electrophoretic movability of particles |
Country Status (4)
Country | Link |
---|---|
JP (1) | JPS5730940A (en) |
DD (1) | DD156337A3 (en) |
DE (1) | DE3113782A1 (en) |
GB (1) | GB2078974A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2580812A1 (en) * | 1985-04-22 | 1986-10-24 | Univ Caen | Method for attenuating or suppressing the phenomenon of electroosmosis in an electrophoresis cell and electrophoresis cell thus obtained |
EP0354984A2 (en) * | 1988-08-19 | 1990-02-21 | Hewlett-Packard Company | Capillary tube with reduced protein interactions and controllable electroosmotic flow |
-
1980
- 1980-07-01 DD DD22229080A patent/DD156337A3/en unknown
-
1981
- 1981-04-04 DE DE19813113782 patent/DE3113782A1/en not_active Withdrawn
- 1981-06-24 JP JP9680581A patent/JPS5730940A/en active Pending
- 1981-06-30 GB GB8120057A patent/GB2078974A/en not_active Withdrawn
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2580812A1 (en) * | 1985-04-22 | 1986-10-24 | Univ Caen | Method for attenuating or suppressing the phenomenon of electroosmosis in an electrophoresis cell and electrophoresis cell thus obtained |
EP0354984A2 (en) * | 1988-08-19 | 1990-02-21 | Hewlett-Packard Company | Capillary tube with reduced protein interactions and controllable electroosmotic flow |
EP0354984A3 (en) * | 1988-08-19 | 1992-09-02 | Hewlett-Packard Company | Capillary tube with reduced protein interactions and controllable electroosmotic flow |
Also Published As
Publication number | Publication date |
---|---|
DE3113782A1 (en) | 1982-01-28 |
JPS5730940A (en) | 1982-02-19 |
DD156337A3 (en) | 1982-08-18 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
WAP | Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1) |