GB2078974A - Vessel for determining the electrophoretic movability of particles - Google Patents

Vessel for determining the electrophoretic movability of particles Download PDF

Info

Publication number
GB2078974A
GB2078974A GB8120057A GB8120057A GB2078974A GB 2078974 A GB2078974 A GB 2078974A GB 8120057 A GB8120057 A GB 8120057A GB 8120057 A GB8120057 A GB 8120057A GB 2078974 A GB2078974 A GB 2078974A
Authority
GB
United Kingdom
Prior art keywords
coating
vessel
methyl cellulose
electrophoretic
particles
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB8120057A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jenoptik AG
Original Assignee
Carl Zeiss Jena GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carl Zeiss Jena GmbH filed Critical Carl Zeiss Jena GmbH
Publication of GB2078974A publication Critical patent/GB2078974A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Electrochromic Elements, Electrophoresis, Or Variable Reflection Or Absorption Elements (AREA)
  • Optical Measuring Cells (AREA)

Abstract

In a vessel for determining the electrophoretic movability of particles, the inner wall of the vessel is coated with a substituted triethoxysilane, preferably aminopropyltriethoxysilane and methyl cellulose, prepared by applying a coating of a substituted triethoxysilane 2 to the interior wall of the vessel 1, applying a coating of methyl cellulose 3 to the coating of substituted triethoxysilane, and repeating this sequence of coatings at least once; the same or a different substituted triethoxy silane being used in each such sequence. <IMAGE>

Description

SPECIFICATION Vessel for determining the electrophoretic movability of particles The invention relates to all vessels for electrophoretic apparata. The invention can be used in all cell electrophoresis methods such as cell electrophoresis, free-flow electrophoresis, in all detection methods such as the laser Doppler principle and in optomechanical simulations of the same, and in all analytic and preparative methods.
It is known to coat the interior walls of vessels with a 5% albumen solution (K. Hannig. et.al. Hoppe Seyler's Journal for Physiological Chemistry 356 (1975) 1209). This solution has the disadvantage that only a slight improvement in the electroosmotic profile is obtained. It is also a disadvantage that this coating is not stable with respect to time and can become detached when the vessel is cleaned for example with 30% formic acid). It is known to coat the interior walls of the vessel with methyl cellulose.
However, this coating can only be applied to unused or freshly cleaned interior walls of vessels. Very flat electroosmotic profiles of the vessels are thereby obtained. However, the profile is less stable. Again this coating becomes detached when the vessel is cleaned (for example with diluted formic acid).
It is also known to coat the interior walls of the vessels with 1% aminopropyltriethoxysilane (K. Hannig et.al). Although this coating is more stable with respect to time, it does not result in electroosmotic profiles which are as flat as those which occur when using methyl cellulose.
The purpose of the invention is to provide a vessel for determining the electrophoretic movability of particles and which renders it possible to determine the electrophoretic movability of particles as accurately as possible with high productivity, a low concentration of the particles to be measured, and small sample volumes.
The object of the invention is to provide a vessel for determining the electrophoretic movability of particles having a flat electroosmotic profile which is stable with respect to time. In accordance with the invention, this object is achieved by a vessel, for determining the electrophoretic movability of particles, which is coated with aminopropyltriethoxysilane and/or methyl cellulose characterised in that a coating of substituted triethoxysilane such as cyclohexenylethyl- or glycidoxypropyl triethoxysilane is applied to the interior wall of the vessel, a coating of methyl cellulose is applied to the coating of substituted triethoxysilane, and that this sequence of coatings is repeated at least once, the same or a different substituted triethoxysilane being used in each such sequence.
The invention will be further described with reference to the drawing which shows, diagrammatically, the arrangement of the coatings on the vessel for determining the electrophoretic movability of particles.
A coating 2 of cyclohexenylethyltriethoxysilane is applied to the interior wall 1 of the vessel. A coating 3 of methyl cellulose is applied to the aforesaid coating.
A coating 4 of aminopropyltriethoxysilane is applied to the coating 3 of methyl cellulose. A coating 3 of methyl cellulose is applied to the coating 4 of aminopropyltriethoxysilane, and a coating 5 of glycidoxypropyltriethoxysilane is applied to the coating 3 of methyl cellulose. A coating 3 of methyl cellulose is applied to the coating 5 of glycidoxypropyltriethoxysilane.
Alternatively, it is possible to use parts of the stated sequence of coatings.
1. A vessel for determining the electrophoretic movability of particles, the inner wall of which vessel is coated with aminopropyltriethoxysilane and/or methyl cellulose, characterised in that a coating of a substituted triethoxysilane is applied to the interior wall of the vessel, a coating of methyl cellulose is applied to the coating of substituted triethoxysilane, and that this sequence of coatings is repeated at least once, the same or a different substituted triethoxy silane being used in each such sequence.
2. A vessel as claimed in claim 1, in which the substituted triethoxy silane used to form at least one of the coatings is cyclohexenylethyl triethoxysilane.
3. A vessel as claimed in claim 1 or 2, in which the substituted triethoxy silane used to form at least one of the coatings is glycidoxypropyl triethoxysilane.
4. A vessel as claimed in claim 1 substantially as hereinbefore described with reference to and as illustrated in the accompanying drawing.
**WARNING** end of DESC field may overlap start of CLMS **.

Claims (4)

**WARNING** start of CLMS field may overlap end of DESC **. SPECIFICATION Vessel for determining the electrophoretic movability of particles The invention relates to all vessels for electrophoretic apparata. The invention can be used in all cell electrophoresis methods such as cell electrophoresis, free-flow electrophoresis, in all detection methods such as the laser Doppler principle and in optomechanical simulations of the same, and in all analytic and preparative methods. It is known to coat the interior walls of vessels with a 5% albumen solution (K. Hannig. et.al. Hoppe Seyler's Journal for Physiological Chemistry 356 (1975) 1209). This solution has the disadvantage that only a slight improvement in the electroosmotic profile is obtained. It is also a disadvantage that this coating is not stable with respect to time and can become detached when the vessel is cleaned for example with 30% formic acid). It is known to coat the interior walls of the vessel with methyl cellulose. However, this coating can only be applied to unused or freshly cleaned interior walls of vessels. Very flat electroosmotic profiles of the vessels are thereby obtained. However, the profile is less stable. Again this coating becomes detached when the vessel is cleaned (for example with diluted formic acid). It is also known to coat the interior walls of the vessels with 1% aminopropyltriethoxysilane (K. Hannig et.al). Although this coating is more stable with respect to time, it does not result in electroosmotic profiles which are as flat as those which occur when using methyl cellulose. The purpose of the invention is to provide a vessel for determining the electrophoretic movability of particles and which renders it possible to determine the electrophoretic movability of particles as accurately as possible with high productivity, a low concentration of the particles to be measured, and small sample volumes. The object of the invention is to provide a vessel for determining the electrophoretic movability of particles having a flat electroosmotic profile which is stable with respect to time. In accordance with the invention, this object is achieved by a vessel, for determining the electrophoretic movability of particles, which is coated with aminopropyltriethoxysilane and/or methyl cellulose characterised in that a coating of substituted triethoxysilane such as cyclohexenylethyl- or glycidoxypropyl triethoxysilane is applied to the interior wall of the vessel, a coating of methyl cellulose is applied to the coating of substituted triethoxysilane, and that this sequence of coatings is repeated at least once, the same or a different substituted triethoxysilane being used in each such sequence. The invention will be further described with reference to the drawing which shows, diagrammatically, the arrangement of the coatings on the vessel for determining the electrophoretic movability of particles. A coating 2 of cyclohexenylethyltriethoxysilane is applied to the interior wall 1 of the vessel. A coating 3 of methyl cellulose is applied to the aforesaid coating. A coating 4 of aminopropyltriethoxysilane is applied to the coating 3 of methyl cellulose. A coating 3 of methyl cellulose is applied to the coating 4 of aminopropyltriethoxysilane, and a coating 5 of glycidoxypropyltriethoxysilane is applied to the coating 3 of methyl cellulose. A coating 3 of methyl cellulose is applied to the coating 5 of glycidoxypropyltriethoxysilane. Alternatively, it is possible to use parts of the stated sequence of coatings. CLAIMS
1. A vessel for determining the electrophoretic movability of particles, the inner wall of which vessel is coated with aminopropyltriethoxysilane and/or methyl cellulose, characterised in that a coating of a substituted triethoxysilane is applied to the interior wall of the vessel, a coating of methyl cellulose is applied to the coating of substituted triethoxysilane, and that this sequence of coatings is repeated at least once, the same or a different substituted triethoxy silane being used in each such sequence.
2. A vessel as claimed in claim 1, in which the substituted triethoxy silane used to form at least one of the coatings is cyclohexenylethyl triethoxysilane.
3. A vessel as claimed in claim 1 or 2, in which the substituted triethoxy silane used to form at least one of the coatings is glycidoxypropyl triethoxysilane.
4. A vessel as claimed in claim 1 substantially as hereinbefore described with reference to and as illustrated in the accompanying drawing.
GB8120057A 1980-07-01 1981-06-30 Vessel for determining the electrophoretic movability of particles Withdrawn GB2078974A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DD22229080A DD156337A3 (en) 1980-07-01 1980-07-01 KUEVETTE FOR DETERMINING THE ELECTROPHORETIC MOBILITY OF PARTICLES

Publications (1)

Publication Number Publication Date
GB2078974A true GB2078974A (en) 1982-01-13

Family

ID=5525074

Family Applications (1)

Application Number Title Priority Date Filing Date
GB8120057A Withdrawn GB2078974A (en) 1980-07-01 1981-06-30 Vessel for determining the electrophoretic movability of particles

Country Status (4)

Country Link
JP (1) JPS5730940A (en)
DD (1) DD156337A3 (en)
DE (1) DE3113782A1 (en)
GB (1) GB2078974A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2580812A1 (en) * 1985-04-22 1986-10-24 Univ Caen Method for attenuating or suppressing the phenomenon of electroosmosis in an electrophoresis cell and electrophoresis cell thus obtained
EP0354984A2 (en) * 1988-08-19 1990-02-21 Hewlett-Packard Company Capillary tube with reduced protein interactions and controllable electroosmotic flow

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2580812A1 (en) * 1985-04-22 1986-10-24 Univ Caen Method for attenuating or suppressing the phenomenon of electroosmosis in an electrophoresis cell and electrophoresis cell thus obtained
EP0354984A2 (en) * 1988-08-19 1990-02-21 Hewlett-Packard Company Capillary tube with reduced protein interactions and controllable electroosmotic flow
EP0354984A3 (en) * 1988-08-19 1992-09-02 Hewlett-Packard Company Capillary tube with reduced protein interactions and controllable electroosmotic flow

Also Published As

Publication number Publication date
DE3113782A1 (en) 1982-01-28
JPS5730940A (en) 1982-02-19
DD156337A3 (en) 1982-08-18

Similar Documents

Publication Publication Date Title
Cappelletti et al. A new electrophoretic method for the complete separation of all known animal glycosaminoglycans in a monodimensional run
Hjertén Free zone electrophoresis
US4456513A (en) Method of and apparatus for measuring electrophoretic mobility
USRE24752E (en) Method of electrophoresis of serum proteins
Tooze et al. The occurrence and possible significance of haemoglobin in the chromosomal regions of mature erythrocyte nuclei of the newt Triturus cristatus cristatus
DE58909398D1 (en) Process for generating a constant discharge potential and for pH value measurement and pH measuring chain in analytical chemistry.
Smith et al. A New Electrophoresis Cell for Microscopic Observations.
Nader et al. Determination of sulfate after chromatography and toluidine blue complex formation
Wehr et al. [16] Capillary isoelectric focusing
GB2078974A (en) Vessel for determining the electrophoretic movability of particles
Plewinsky et al. Sodium metatungstate, a new medium for binary and ternary density gradient centrifugation
Peniston et al. Ionophoretic analyses in agar gels
Cross et al. Salt effects in capillary zone electrophoresis I. Dependence of electrophoretic mobilities upon the hydrodynamic radius
Saito et al. Ionic mobility of the solvated proton and acid–base titration in a four-compartment capillary electrophoresis system
US4911808A (en) Mobilization of focused protein zones by ion introduction
Lim et al. Measurement of diffusion coefficient and electrophoretic mobility with a quasielastic light‐scattering–band‐electrophoresis apparatus
US2748074A (en) Electrophoretic microcell
Hjertén Zone electrophoresis, isoelectric focusing, and displacement electrophoresis (isotachophoresis) in carrier-free solution
Smith et al. Direct Titration of Potassium with Tetraphenylborate. Amperometric Equivalence-Point Detection
JPH05232029A (en) Method and apparatus for ph-value measurement by fluorescent photometry
JPS57196145A (en) Measurement of ion concentration
JPS5631641A (en) New quantitative determination method for hydrogen peroxide
DE59002545D1 (en) With an elongated machine part, in particular the scraper beam of a coating device, a device for measuring deflections which is firmly connected.
Denzumi et al. A new basicity scale based on redox potential of Cr6+/Cr3+ in alkali silicate melts
BR8008920A (en) PROCESS AND APPARATUS FOR DETERMINING THE CONCENTRATION OF A SUBSTANCE CONNECTED WITH PARTICLES CONDUCTED BY A FLUENT MEDIUM

Legal Events

Date Code Title Description
WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)