GB2072130A - Device for storing frozen plasma - Google Patents

Device for storing frozen plasma Download PDF

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Publication number
GB2072130A
GB2072130A GB8006363A GB8006363A GB2072130A GB 2072130 A GB2072130 A GB 2072130A GB 8006363 A GB8006363 A GB 8006363A GB 8006363 A GB8006363 A GB 8006363A GB 2072130 A GB2072130 A GB 2072130A
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GB
United Kingdom
Prior art keywords
container
plasma
frozen
compartments
substance
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB8006363A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CORRALL R J M
Original Assignee
CORRALL R J M
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CORRALL R J M filed Critical CORRALL R J M
Priority to GB8006363A priority Critical patent/GB2072130A/en
Publication of GB2072130A publication Critical patent/GB2072130A/en
Withdrawn legal-status Critical Current

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Classifications

    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
    • F25D25/00—Charging, supporting, and discharging the articles to be cooled
    • F25D25/005—Charging, supporting, and discharging the articles to be cooled using containers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
    • A01N1/0268—Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen

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  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Mechanical Engineering (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Combustion & Propulsion (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Medical Preparation Storing Or Oral Administration Devices (AREA)

Abstract

A device suitable for storing plasma comprises an elongate container having along its length a plurality of compartments interconnected by necks of reduced cross-sectional area. Plasma is introduced in liquid form into the container and then frozen therein. When an aliquot of plasma is required, the container is cut at an appropriate neck and the plasma in that part of the container which is cut off is then thawed, whereas the plasma in the other part of the container is kept frozen for future use. <IMAGE>

Description

SPECIFICATION A device to facilitate the processing of frozen plasma in medical research Underlying Principle In biological research and in hospital laboratory practice samples of plasma are frequentiy kept frozen prior to assay and maintained at sub-zero temperatures so that chemicals in the plasma (hormones, biochemical substrates, enzymes) will not decompose prior to assay of the chemical. The thawing of plasma samples requires the thawing of the whole sample and with this change of physical state many chemicals in plasma undergo decomposition rendering the rest of the sample useless. For example 10 ml of plasma may require thawing to provide 0.1 ml for an assay and the remaining 9.9 ml then is discarded. One way of circumventing this problem is to split plasma samples into several individual plastic tubes each separately labelled.These may then be thawed individually and used as required. This method is however costly, requires careful stacking of many tubes, is time consuming particularly with regard to the labelling of tubes and there is a conjectural element in estimating the size(s) of individual tubes that need to be filled, labelled and frozen.
The present invention is a device that would enable volumes of plasma to be thawed in units of the required volume for subsequent assay without recourse to thawing the whole sample and therefore it avoids wastage of valuable plasma.
The Device The device (fig. 1 ) consists of a container with multiple compartments made of plastic (or similar material capable of freezing) in which the compartments (section A) are connected by tubelike channels (section B). Plasma is put in to the container filling the compartments (allowing some space for expansion) and the whole container is frozen in a horizontal position. The container may then be labelled and stored at a suitable sub-zero temperature. As aliquots of plasma are required they may be cut off with scissors through the appropriate connecting tube to give the volume of plasma required leaving the remainder in the frozen state which is returned to the freezer. The cut-off sample would be allowed to thaw in to a separate container, draining through the connecting tube like a funnel (fig. 1 C).More than one size of device could be manufactured with separate sized (e.g. 0.1 ml, 0.5 ml, 1 ml, 2 ml, 5 ml) or different numbers of compartments (e.g.
5, 10 or even 20). The details of the design could vary, e.g. the shape of the individual compartments and of the connecting tubes (e.g.
1 D-F). The inventor knows of no similar device available at present. The device has been developed in response to a need experienced by the applicant when engaged in medical research involving samples of frozen plasma. The exact details of design and dimensions would be determined to a large extent by trial and error with prototype models. After cutting the connecting tubes to take a sample the cut end could be covered by a simple plastic stopper of appropriate size although this might be unnecessary.
1. A device suitable for use in storing plasma or another liquid substance in a frozen condition, the device comprising an elongate container formed of a material capable of being severed and of withstanding a temperature of less than OOC, the container having along its length a plurality of compartments interconnected by necks each having a cross-sectional area less than that of an adjacent compartment, the arrangement being such that, in use, with a frozen substance in the container, a neck can be severed to provide an aliquot of the substance in frozen condition which can subsequently be thawed and removed from its compartment.
2. A device according to Claim 1, wherein the container is formed of a plastics material.
3. A device according to Claim 1 or 2, wherein the container is closed at one end and open at the other end.
4. A device according to Claim 3, which also includes a closure member for the open end of the container.
5. A device according to any preceding claim, wherein the container includes provision for labelling.
6. A device according to any preceding claim, wherein the volume of each compartment is approximately 0.1, 0.5, 1, 2 or 5 ml.
7. A device according to any preceding claim, wherein the container has from 5 to 20 compartments.
8. A device according to any preceding claim, wherein the compartments and the necks are of circular cross-section.
9. A device according to any one of Claims 1 to 7, wherein the compartments and the necks are of non-circular cross-section.
10. A device according to Claim 9, wherein the compartments and necks are of oblong rectangular cross-section.
11. A device according to any preceding claim, wherein the container has a main axis with the necks being coaxial with that main axis.
12. A device according to any one of Claims 1 to 10, wherein the container has a main axis with the necks lying off that main axis.
1 3. A device according to claim 1, substantially as hereinbefore described with reference to, and as illustrated in, the accompanying drawing.
1 4. A method of storing and otherwise handling plasma or another liquid substance, which method comprises:-- introducing, into the container of a device as claimed in any preceding claim, plasma or another liquid substance In a liquid condition; lowering the temperature so as to cause the substance to freeze within the container; then, when an aliquot of the substance is required, severing the container in the region of a neck whilst the substance is still frozen; maintaining in
**WARNING** end of DESC field may overlap start of CLMS **.

Claims (12)

**WARNING** start of CLMS field may overlap end of DESC **. SPECIFICATION A device to facilitate the processing of frozen plasma in medical research Underlying Principle In biological research and in hospital laboratory practice samples of plasma are frequentiy kept frozen prior to assay and maintained at sub-zero temperatures so that chemicals in the plasma (hormones, biochemical substrates, enzymes) will not decompose prior to assay of the chemical. The thawing of plasma samples requires the thawing of the whole sample and with this change of physical state many chemicals in plasma undergo decomposition rendering the rest of the sample useless. For example 10 ml of plasma may require thawing to provide 0.1 ml for an assay and the remaining 9.9 ml then is discarded. One way of circumventing this problem is to split plasma samples into several individual plastic tubes each separately labelled.These may then be thawed individually and used as required. This method is however costly, requires careful stacking of many tubes, is time consuming particularly with regard to the labelling of tubes and there is a conjectural element in estimating the size(s) of individual tubes that need to be filled, labelled and frozen. The present invention is a device that would enable volumes of plasma to be thawed in units of the required volume for subsequent assay without recourse to thawing the whole sample and therefore it avoids wastage of valuable plasma. The Device The device (fig. 1 ) consists of a container with multiple compartments made of plastic (or similar material capable of freezing) in which the compartments (section A) are connected by tubelike channels (section B). Plasma is put in to the container filling the compartments (allowing some space for expansion) and the whole container is frozen in a horizontal position. The container may then be labelled and stored at a suitable sub-zero temperature. As aliquots of plasma are required they may be cut off with scissors through the appropriate connecting tube to give the volume of plasma required leaving the remainder in the frozen state which is returned to the freezer. The cut-off sample would be allowed to thaw in to a separate container, draining through the connecting tube like a funnel (fig. 1 C).More than one size of device could be manufactured with separate sized (e.g. 0.1 ml, 0.5 ml, 1 ml, 2 ml, 5 ml) or different numbers of compartments (e.g. 5, 10 or even 20). The details of the design could vary, e.g. the shape of the individual compartments and of the connecting tubes (e.g. 1 D-F). The inventor knows of no similar device available at present. The device has been developed in response to a need experienced by the applicant when engaged in medical research involving samples of frozen plasma. The exact details of design and dimensions would be determined to a large extent by trial and error with prototype models. After cutting the connecting tubes to take a sample the cut end could be covered by a simple plastic stopper of appropriate size although this might be unnecessary. CLAIMS
1. A device suitable for use in storing plasma or another liquid substance in a frozen condition, the device comprising an elongate container formed of a material capable of being severed and of withstanding a temperature of less than OOC, the container having along its length a plurality of compartments interconnected by necks each having a cross-sectional area less than that of an adjacent compartment, the arrangement being such that, in use, with a frozen substance in the container, a neck can be severed to provide an aliquot of the substance in frozen condition which can subsequently be thawed and removed from its compartment.
2. A device according to Claim 1, wherein the container is formed of a plastics material.
3. A device according to Claim 1 or 2, wherein the container is closed at one end and open at the other end.
4. A device according to Claim 3, which also includes a closure member for the open end of the container.
5. A device according to any preceding claim, wherein the container includes provision for labelling.
6. A device according to any preceding claim, wherein the volume of each compartment is approximately 0.1, 0.5, 1, 2 or 5 ml.
7. A device according to any preceding claim, wherein the container has from 5 to 20 compartments.
8. A device according to any preceding claim, wherein the compartments and the necks are of circular cross-section.
9. A device according to any one of Claims 1 to 7, wherein the compartments and the necks are of non-circular cross-section.
10. A device according to Claim 9, wherein the compartments and necks are of oblong rectangular cross-section.
11. A device according to any preceding claim, wherein the container has a main axis with the necks being coaxial with that main axis.
12. A device according to any one of Claims 1 to 10, wherein the container has a main axis with the necks lying off that main axis.
1 3. A device according to claim 1, substantially as hereinbefore described with reference to, and as illustrated in, the accompanying drawing.
1 4. A method of storing and otherwise handling plasma or another liquid substance, which method comprises:-- introducing, into the container of a device as claimed in any preceding claim, plasma or another liquid substance In a liquid condition; lowering the temperature so as to cause the substance to freeze within the container; then, when an aliquot of the substance is required, severing the container in the region of a neck whilst the substance is still frozen; maintaining in the frozen condition the substance in one part of the container; and thawing the substance in that other part of the container which was cut off during the severing, whereby this aliquot of substance may be removed from said part of the container.
1 5. A method, according to Claim 14, of storing and otherwise handling plasma or another liquid substance, substantially as hereinbefore described.
GB8006363A 1980-02-26 1980-02-26 Device for storing frozen plasma Withdrawn GB2072130A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB8006363A GB2072130A (en) 1980-02-26 1980-02-26 Device for storing frozen plasma

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB8006363A GB2072130A (en) 1980-02-26 1980-02-26 Device for storing frozen plasma

Publications (1)

Publication Number Publication Date
GB2072130A true GB2072130A (en) 1981-09-30

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Family Applications (1)

Application Number Title Priority Date Filing Date
GB8006363A Withdrawn GB2072130A (en) 1980-02-26 1980-02-26 Device for storing frozen plasma

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009086829A2 (en) * 2008-01-07 2009-07-16 Hvidovre Hospital A storage vessel and a break tool for dividing such vessel
WO2017032666A1 (en) * 2015-08-21 2017-03-02 1 Cryobio Ag Vessel

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009086829A2 (en) * 2008-01-07 2009-07-16 Hvidovre Hospital A storage vessel and a break tool for dividing such vessel
WO2009086829A3 (en) * 2008-01-07 2010-04-22 Hvidovre Hospital A storage vessel and a break tool for dividing such vessel
US8641990B2 (en) 2008-01-07 2014-02-04 Ove Andersen Storage vessel and a break tool for dividing such vessel
WO2017032666A1 (en) * 2015-08-21 2017-03-02 1 Cryobio Ag Vessel

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