GB2065473A - Process for producing hepatitis b vaccine - Google Patents

Process for producing hepatitis b vaccine

Info

Publication number
GB2065473A
GB2065473A GB8106701A GB8106701A GB2065473A GB 2065473 A GB2065473 A GB 2065473A GB 8106701 A GB8106701 A GB 8106701A GB 8106701 A GB8106701 A GB 8106701A GB 2065473 A GB2065473 A GB 2065473A
Authority
GB
United Kingdom
Prior art keywords
precipitate
vaccine
fraction
hbsag
particles
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB8106701A
Other versions
GB2065473B (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alpha Therapeutic Corp
Original Assignee
Alpha Therapeutic Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alpha Therapeutic Corp filed Critical Alpha Therapeutic Corp
Publication of GB2065473A publication Critical patent/GB2065473A/en
Application granted granted Critical
Publication of GB2065473B publication Critical patent/GB2065473B/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00—Reverse transcribing DNA viruses
    • C12N2730/00011—Details
    • C12N2730/10011—Hepadnaviridae
    • C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

A hepatitis B vaccine is prepared by adjusting the pH of an aqueous suspension of a crude HBsAg to neutral by removing plasma protein components. Polyethylene glycol is added to a 5-6% concentration to thereby remove Dane particles and immune complexes as a precipitate. A further amount of a polyethylene glycol is added to thereby collect HBsAg particles as a precipitate. A specific fraction of HBsAg particles having a particle size of 22 nm and a specific gravity of 1.19-1.21 g/cm<s3>s is recovered from the precipitate by gel filtration and ultracentrifugation. The virus contained in the fraction is inactivated and the fraction is made into a vaccine preparation.
GB8106701A 1979-07-05 1980-07-03 Process for producing hepatitis b vaccine Expired GB2065473B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP8549379A JPS5610119A (en) 1979-07-05 1979-07-05 Manufacture of b type hepatitis infection preventive vaccine

Publications (2)

Publication Number Publication Date
GB2065473A true GB2065473A (en) 1981-07-01
GB2065473B GB2065473B (en) 1984-02-29

Family

ID=13860454

Family Applications (1)

Application Number Title Priority Date Filing Date
GB8106701A Expired GB2065473B (en) 1979-07-05 1980-07-03 Process for producing hepatitis b vaccine

Country Status (3)

Country Link
JP (1) JPS5610119A (en)
GB (1) GB2065473B (en)
WO (1) WO1981000050A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4554157A (en) * 1981-01-29 1985-11-19 National Research Development Corporation Hepatitis B vaccine

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZW18282A1 (en) * 1981-08-31 1983-03-23 Genentech Inc Preparation of polypeptides in vertebrate cell culture
JPS59101426A (en) * 1982-11-29 1984-06-12 Green Cross Corp:The Preparation of vaccine for preventing infection of b-type hepatitis
FR2560890B1 (en) * 1984-03-07 1987-10-16 Grp Genie Genetique COMPOSITION USEFUL FOR THE MANUFACTURE OF VACCINES CONTAINING PARTICLES CARRYING THE SURFACE ANTIGEN OF HEPATITIS B VIRUS AND THE POLYMERIZED HUMAN SERUM ALBUMIN RECEPTOR, ANIMAL CELLS CAPABLE OF PRODUCING SUCH PARTICLES
FR2561256B1 (en) * 1984-03-16 1987-01-02 Pasteur Institut PROCESS FOR THE PURIFICATION OF BIOLOGICAL PARTICLES OF THE HBS ANTIGEN TYPE, BY ULTRACENTRIFUGATION OF FLOTATION ON DENSITY GRADIENT
JPS60197629A (en) * 1984-03-16 1985-10-07 Chemo Sero Therapeut Res Inst Method of purification of hbs antigen
NL8401066A (en) * 1984-04-04 1985-11-01 Stichting Centraal Lab PROCESS FOR PREPARING PREPARATIONS INCREASING HUMORAL AND CELLULAR IMMUNE ACTIVITY
JPS60258127A (en) * 1984-06-04 1985-12-20 Green Cross Corp:The Preparation of hepatitis b vaccine
EP0294071A3 (en) * 1987-06-03 1990-08-01 Merck & Co. Inc. Hepatitis b vaccines made with pre-formed aluminum hydroxide gels, and processes thereof
EP0384058A1 (en) * 1989-02-09 1990-08-29 Development Center For Biotechnology Isolation of hepatitis B surface antigen from transformed yeast cells
WO1993005811A1 (en) * 1991-09-18 1993-04-01 Amgen Inc. A hepatitis b vaccine formulation incorporating a bile acid salt

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL286954A (en) * 1962-01-03
BR6915288D0 (en) * 1969-03-15 1973-02-08 Bayer Ag PROCESS FOR THE PURIFICATION OF SOLUTIONS OF THE FOOT-AND-Mouth Disease VIRUS
US3989818A (en) * 1970-08-14 1976-11-02 South African Inventions Development Corporation Influenza virus vaccine
GB1356413A (en) * 1971-09-09 1974-06-12 Pfizer Ltd Method of obtaining purified australia antigen from blood serum
US3951937A (en) * 1973-12-20 1976-04-20 The Community Blood Council Of Greater New York, Inc. Large scale purification of hepatitis type B antigen using polyethylene glycol
US3994870A (en) * 1974-01-31 1976-11-30 The Community Blood Council Of Greater New York, Inc. Purification of hepatitis B surface antigen
FR2303562A1 (en) * 1975-03-14 1976-10-08 Community Blood Council Greate NEW ANTIGEN PREPARATION FOR USE AS A VACCINE
SE437329B (en) * 1975-03-14 1985-02-25 Community Blood Council PUT OUT OF BLOOD SERUM TO PROVIDE VACCIN AGAINST VIRUS Hepatitis
FR2336141A1 (en) * 1975-12-23 1977-07-22 Trepo Christian NEW MEDICINE TO TREAT ACUTE OR CHRONIC HEPATITIS B VIRUS INFECTIONS
DE2750045A1 (en) * 1977-11-09 1979-05-10 Behringwerke Ag METHOD FOR REMOVING DETERGENTS FROM VIRUS ANTIGENS SUSPENSIONS

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4554157A (en) * 1981-01-29 1985-11-19 National Research Development Corporation Hepatitis B vaccine

Also Published As

Publication number Publication date
WO1981000050A1 (en) 1981-01-22
JPS5721246B2 (en) 1982-05-06
JPS5610119A (en) 1981-02-02
GB2065473B (en) 1984-02-29

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee