GB2056981A - Benzodiazepines - Google Patents
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- GB2056981A GB2056981A GB8026894A GB8026894A GB2056981A GB 2056981 A GB2056981 A GB 2056981A GB 8026894 A GB8026894 A GB 8026894A GB 8026894 A GB8026894 A GB 8026894A GB 2056981 A GB2056981 A GB 2056981A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
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- C07D243/00—Heterocyclic compounds containing seven-membered rings having two nitrogen atoms as the only ring hetero atoms
- C07D243/06—Heterocyclic compounds containing seven-membered rings having two nitrogen atoms as the only ring hetero atoms having the nitrogen atoms in positions 1 and 4
- C07D243/10—Heterocyclic compounds containing seven-membered rings having two nitrogen atoms as the only ring hetero atoms having the nitrogen atoms in positions 1 and 4 condensed with carbocyclic rings or ring systems
- C07D243/14—1,4-Benzodiazepines; Hydrogenated 1,4-benzodiazepines
- C07D243/16—1,4-Benzodiazepines; Hydrogenated 1,4-benzodiazepines substituted in position 5 by aryl radicals
- C07D243/18—1,4-Benzodiazepines; Hydrogenated 1,4-benzodiazepines substituted in position 5 by aryl radicals substituted in position 2 by nitrogen, oxygen or sulfur atoms
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Abstract
Optically active and racemic 5- phenyl-1,3,4,5-tetrahydro-2H-1,4- benzodiazepin-2-one compounds of general formula (I> <IMAGE> in which the centres of asymmetry in the 3- and 5-positions have the same absolute configuration [wherein R<1> represents a hydrogen or halogen atom or a trifluoromethyl or nitro group; R<2> represents a hydrogen atom or an alkyl group having 1 to 6 carbon atoms; R<3> represents a group selected from those groups conventionally attached to the -CH(NH2)-COOH group of the optically active or racemic alpha - amino-acids; R<4> represents a hydrogen atom or a chlorocarbonyl or carbamoyl group; and X represents a hydrogen or halogen atom or a trifluoromethyl group; with the proviso that if in the racemic compounds R<4> represents a hydrogen atom then R<3> represents other than an alkyl group having 1 to 6 carbon atoms] and acid addition salts thereof show enzyme inducing activity.
Description
SPECIFICATION
Phenyl-tetrahydro-benzodiazepinones, Process for the Preparation and Pharmaceutical
Compositions Thereof
This invention relates to new 5-phenyl-1 ,3,4,5-tetrahydro-2H-1 ,4-benzodiazepin-2-one derivatives and process for their preparation. More particularly, the invention concerns new optically active or racemic 5-phenyl-1 ,3,4,5-tetrahydro-2H-1 ,4-benzodiazepin-2-one derivatives, which contain centres of asymmetry in the 3- and 5-positions and in which said centres are in the same absolute configuration. The invention also includes a process for the preparation of said compounds and pharmaceutical compositions containing them.
The new 5-phenyl-1 ,3,4,5-tetrahydro-2H-1 ,4-benzodiazepin-2-one derivatives according to the invention are encompassed by the general formula (I).
The most closely related tetrahydro-1 ,4-benzodiazepin-2-ones known in the art are unsubstituted in the 4-position and contain a lower alkyl group in the 3-position. Their preparation is for example disclosed in the following publications: German Patent Specification No. 1,199,776 describes the catalytic hydrogenation of the corresponding dihydro-derivatives, and according to U.S. Patent
Specification No. 3,522,289 the compounds are prepared by intramolecular condensation of the aminoacetic acid esters N-substituted by an 2-amino-benzhydryl group.Austrian Patent Specification
No. 1,1 99,776 discloses a method along which the protecting group of the corresponding 2aminobenzhydrole derivatives acylated by protected amino acids is split off by acidolysis, while according to Austrian Patent Specification No. 311,356 the compounds are prepared from the corresponding aminobenzophenone derivatives acylated by protected amino acids by catalytic hydrogenation. Finally, Austrian Patent Specification No. 309,439 discloses the resolution of racemic tetrahydro-1 ,4-benzodiazepin-2-ones containing a centre of asymmetry in the 5-position through the preparation of salts.
The compounds of general formula (I), as defined hereinafter, have been found to possess valuable enzyme inducing activity and have also been found to be practicaliy devoid of the sedative effect of the related compounds (such as those discussed above).
According to one aspect of the present invention we therefore provide optically active or racemic 5-phenyl- 1 ,3,4,5-tetrahydro-2H- 1 ,4- benzodiazepin-2-one compounds of general formula (I)
in which the centres of asymmetry in the 3- and 5-positions have the same absolute configuration [wherein
R1 represents a hydrogen or halogen atom or a trifluoromethyl or nitro group;
R2 represents a hydrogen atom or a alkyl group having 1 to 6 carbon atoms;
R3 represents a group selected from those groups conventionally attached to the --CH(NH,))-COOH group of the optically active or racemic a-amino-acids;
R4 represents a hydrogen atom or a chlorocarbonyl or carbamoyl group; and
X represents a hydrogen or halogen atom or a trifluoromethyl group; with the proviso that if in the racemic compounds R4 represents a hydrogen atom then R3 represents other than an alkyl group having 1 to 6 carbon atoms] acid addition salts thereof.
The pharmaceutically acceptable acid addition salts of the compounds of the general formula (I) are deemed advantageous within the scope of the present invention.
The term "halogen" used throughout the specification relates to fluorine, chlorine, bromine or iodine.
The term "alkyl group having 1 to 6 carbon atoms" as used herein means straight or branched chained aliphatic hydrocarbon groups, having 1 to 6 carbon atoms (e.g. methyl, ethyl, n-propyl, isopropyl, n-butyl, sec.-butyl, tert.-butyl, n-pentyl, isopentyl, n-hexyl or isohexyl, etc.).
The term "groups conventionally attached to the --CH(NH,))-COOH group of the optically active or racemic a-amino-acids" preferably relates to optionally substituted lower alkyl groups and advantageously relates to groups such as for example methyl, isopropyl, benzyl-4-hydroxybenzyl and 3-indolyl-methyl.
5 10 15
20
25 30 35 40 45 50
It has surprisingly been found that by reducing dihydro-1 ,4-benzodiazepin-2-one derivatives containing a centre of asymmetry in the 3-position new tetrahydro-1 ,4-benzodiazepin-2-ones can be prepared, which contain centres of asymmetry in the 3- and 5-positions and in which the the absolute configuration of the centre in the 5-position is identical with the absolute configuration of the centre in the 3-position which is present also in the starting material.
According to a further aspect of the present invention we provide a process for the preparation of racemic or optically active 5-phenyl-1 ,3,4,5-tetrahydro-2H-1 ,4-benzodiazepin-2-one compounds of general formula (I) (wherein R1, R2, R3 and X are as defined above and R4 represents a hydrogen atom), in which the centres of asymmetry in the 3- and 5-positions have the same absolute configuration, and acid addition salts thereof, which process comprises reducing racemic or optically active 5-phenyl-1,3dihydro-2H-1 ,4-diazepin-2-one compounds of general formula (II)
(wherein R1, R2, R3 and X are as defined above) having a centre of asymmetry in the 3-position and, if desired, converting the product into an acid addition salt thereof.
The process according to the invention is stereospecific, i.e. starting from the 3S, 3R and 3SR dihydro-compounds of the general formula (II), respectively the corresponding 3S, 5S; 3R, 5R and 3SR, 5SR tetrahydro-compounds, respectively can be prepared without resolution.
The compounds of the general formula (11) (wherein R1, R2, R3 and X are as hereinbefore defined) used as starting compounds in the process according to the invention can be prepared by the process disclosed in Austrian Patent Specification No. 281,035.
The compounds of the general formula (II) can be reduced by reducing agents, which are capable of saturating the 4,5-double bond (azomethine group), without influencing other parts of the molecule.
The reduction can for example be carried out with a complex metal hydride, such as sodium borohydride, with a metal and an acid, such as zinc and acetic acids, with nascent hydrogen or by catalytic hydrogenation wherein as a catalyst any conventional metal on the surface of a carrier, such as palladium-on-charcoal catalyst or a metal oxide, such as platinum oxide can be used.
The reduction of the compounds of the general formula (II) is performed in a reaction inert organic solvent, such as an aliphatic alcohol having 1 to 6 carbon atoms, e.g. methanol, ethanol; or an aliphatic carboxylic acid having 1 to 6 carbon atoms, e.g. acetic acid, etc.
The reaction temperature can be varied within a wide range but preferably is between OOC and 1 500 C, more preferably about room temperature. The reaction time strongly depends on the starting compound and solvent employed and on the reaction temperature, and generally is about 1 to 24 hours, preferably 0.5 to 8 hours.
The compounds of the general formula (I), in which R4 represents a hydrogen atom can be converted into acid addition salts by reacting with corresponding acids. The preparation of salts can be used also for the purification of the compounds, and from the acid addition salts obtained, optionally after recrystallisation, the compounds of the general formula (I) can be set free by known methods.
For the preparation of salts for example the following acids can be used: inorganic acids, such as hydrogen halides, e.g. hydrochloric acid, hydrogen bromide; sulfuric acid; phosphoric acid; nitric acid; or perhaloic acids, such as perchloric acid; or organic acids, such.as formic acid, acetic acid, propionic acid, glycolic acid, maleic acid, hydroxymaleic acid, fumaric acid, salicylic acid, lactic acid, cinnamic acid, benzoic acid, phenylacetic acid, p-amino-benzoic acid, p-hydrobenzoic acid, p-amino-salicylic acid, etc.; alkylsulfonic acids, such as methanesulfonic acid, ethanesulfonic acid, etc.; cycloaliphatic sulfonic acids, such as cyclohexanesulfonic acid; arylsulfonic acids, such asp-toluene-sulfonic acid, naphthyl-sulfonic acid, sulfanilic acid, etc.; amino acids, such as asparginic acid, glutaminic acid, etc.
The acid addition salts can be prepared in any conventional inert organic solvent, which is capable of dissolving the compound of the general formula (I) used as starting compound, and in which the acid addition salt of said compound is insoluble. In this case the acid addition salt precipitated from the reaction mixture can easily be separated by conventional techniques, e.g. filtration. Alternatively, the preparation of acid addition salts can be carried out also in inert organic solvents, in which not only the compounds of the general formula (I) but also their acid addition salts are soluble. In this case the acid addition salt can be precipitated by an apolar organic solvent, e.g. by petroleum ether.
The compounds of the general formula (I) in which R4 stands for a hydrogen atom are preferably reacted with alkali metal cyanates via their acid addition salts. The corresponding compound of the general formula (I) (wherein R4 represents hydrogen) is converted into an acid addition salt, preferably hydrogen halide thereof, the salt obtained is separated and suspended or dissolved in an inert organic solvent, such as acetic acid, whereupon the alkali metal cyanate is added to the suspension or solution obtained. As an alkali metal cyanate potassium or sodium cyanate can for example be used.
The reaction temperature can be varied within a wide range but the reaction is preferably performed at about room temperature. The reaction time generally is between 0.5 and 10 hours, depending on the starting compounds, solvent and temperature.
The reaction of the compounds of the general formula (I) (wherein R1, R2, R3 and X are as defined above and R4 stands for hydrogen) with phosgene is carried out in an inert organic solvent, such as aromatic hydrocarbons, e.g. benzene, in the presence of an acid binding agent, such as magnesium oxide or sodium hydrogencarbonate. The compounds of the general formula (I) obtained, in which R4 stands for a chlorocarbonyl group (R', R2, R3 and X are as defined above) are treated with a concentrated aqueous ammonium hydroxide solution or with a solution of ammonia in an aliphatic alcohol having 1 to 6 carbon atoms, preferably methanol, optionally in an inert organic solvent, e.g. an aliphatic alcohol having 1 to 6 carbon atoms.Alternatively, the ammonia solution can also be added to the reaction mixture obtained when preparing the compounds of the general formula (I), in which R4 stands for a chlorocarbonyl group, i.e. the compounds of the general formula (I) need not necessarily be isolated before reaction with ammonia.
The compounds of the general formula (I), in which R2 stands for hydrogen (R', R3, R4 and X are as hereinabove defined) can be converted into the corresponding 1-alkyl derivatives by reacting with an alkylating agent. As alkylating agents conventional reactants, such as alkyl halides, preferably alkyl iodide, or dialkyl sulfates can be used. Before carrying out the alkylation the compounds of the general formula (I) are preferably converted into alkali metal derivatives thereof. In this case the compounds are dissolved in an inert organic solvent, such as dioxane, dimethyl formamide, benzene or toluene and are reacted with an alkali metal, alkali metal hydride or alkali metal amide, preferably with sodium or sodium hydride or sodium amide at O to 1 500C. The alkali metal compound obtained is then reacted with the corresponding alkylating agent.
The new compounds of the general formula (I) can be prepared by the process according to the invention with a good yield, in a well identifiable form. The results of chemical analysis are in good agreement with the calculated values.
The purity of the compounds was controlled by thin layer chromatography. The retention factors (Rf) of the compounds illustrated in the working examples were determined on a Stahl GF 254 (Merck) silica gel plate by a 1 :1 mixture of ether and dichloromethane. The detection was performed in U.V.
light at 254 nm. The melting points were determined in an equipment according to dr. Tottoli (noncorrected values). For the structural analysis IR, circular dichroism or NMR spectroscopy was used.
The pharmacological properties of the racemic or optically active 1 ,3,4,5-tetrahydro-2H-1 4- benzodiazepin-2-one derivatives according to the invention, which contain two centres of asymmetry, first of all their excellent enzyme inducing activity, the significant reduction of the sedative effect and their low toxicity are illustrated by the following pharmacological tests.
The biological activity and duration of activity of different endogenic and exogenic compounds are greatly influenced by the activity of the NADPH- (nicotinic acid amide-adenine-dinucleotide phosphate-) dependent multifunctional oxidase enzyme system of the liver. There are numerous compounds known in the art, showing various pharmacological activities, which are capable of increasing or inducing the activity of the metabolising, multifunctional oxidase enzyme system of the liver [see e.g. Sher, S. P.: Toxicol. appl. Pharmacol. 18, 780 (1971)]; G. J. Mannering: in A. Burger's
Selected Pharmacological Testing Methods, 51-119 S., Marcel Dekker Inc., New York (1968)]. It is well known that phenobarbital shows an inducing activity in the case of human diseases caused by the defectiveness of the metabolising enzyme system of the liver.Therefore phenobarbital can successfully be used in the treatment of Czigler-Najjar and Cilbert syndromes and neunatalis hyperbilirubinemia, though due to its hypnotic-sedative effect the inducing activity of this compound is not optimal [Vessel and J. E. Page: J. Clin. Invest. 48, 2202 (1 969; J. T. Wilson: Pediatrics 43, 424 (1 969)].
For screening the enzyme inducing activity the change in the hexobarbital-induced sleeping time is conventionally measured. The inducing activity of the compounds of the present invention was compared to the corresponding activity of phenobarbital and to their own sedative effect, using the following test methods.
Determination of the Sleeping Time Induced by Hexobarbital (Measurement of Enzyme Inducing
Activity)
After a) one hour and b) after 24 hour pretreatment with a 40 mg/kg p.o. dose of the test compounds, the groups of test animals'were administered a 60 mg/kg i.v. dose of hexobarbital. The average sleeping time (+SD) and the percentage change related to the control (%) are given in Table 1 below.
DePsPaumination of the Potentiation of Activity of Sodium-barbital (Measurement of the Sedative
Activity)
Na-barbital is not metabolised in the liver, thus the potentiation of the activity of Na-barbital can be considered as a direct CNS effect. In the tests carried out [see S. Goldschmidt and R. Wohr: Z.
physiol. Chem. 308, 9 (1957); D. V. Parker: J. Pharm. Pharmac. 27, 729 (19750] the test animals were pretreated with a 20 mg/kg i.p. dose of the test compounds of the general formula (I) and after one hour were administered a 100 mg/kg i.p. dose of Na-barbital. The administered (100 mg/kg) dose of
Na-barbital still has no narcotic effect. The number of the animals falling asleep (in %) is given in Table 2.
Acute Toxicity (p.o.)
To the test animals 250 and 500 mg/kg doses, resp. of the test compounds were administered orally and the number of the dead animals was registered for 14 days. The results are shown in Table 3.
As test animals CFLP male mice weighing 8 to 22 g were used.
Table 1
Hexobarbital-sleeping Time (mien) Test Compound Dose mg/kg 1 Hour Pretreatment 24 Hour Pretreatment
(Example No.) p.o. Average+SE/N% Average+SEA% Control 0 35+2.38 34+2.60
1 40 67.2+7.01+91X 19.5+1.84-43 2 40 61.2+5.21+73X 133+0.54X - 61 3 40 43.3+4.18+23 12.0+0.83X-65 4 40 43.3+4.01+23 9.9+0.62X - 71 5 40 37.3+3.01 +6 9+0.43X73 Phenobarbital 40 86.2+7.51+145 1 11.2+0.35X - 69 ("p < 0.001 compared to the control.)
Table 2
Number of the Test
Text Compound Dose mg/kg Animals Fallen Asleep
(Example No.) i.p. in % of the Control
1 20 50
2 20
3 20 0
4 20
5 20 20
Phenobarbital 20 60
Table 3
Test Compound LD50 mg/kg
(Example No.) p.o.
1 < 500
2 < 500
3 < 500
4 < 500
5 < 500
Phenobarbital 190.9
The known compounds having enzyme inducing activity generally show an inhibiting effect
immediately after administration, which results in the prolongatibn of the sleeping time.
From the data listed in Table 1 it can clearly be seen that in the case of the compounds according to the invention the sleeping time determined after 1 hour after administration is only slightly increased. In this respect the compounds of Examples 3, 4 and 5 have the most favourable properties.
It can also be seen that phenobarbital increases the sleeping time to almost 3 times of its original value. 24 hours after administration the compounds according to the invention show the same enzyme inducing activity as phenobarbital, therefore the decomposition of hexobarbital in the organism is accelerated and accordingly the sleeping time is reduced.
The sleeping time induced by hexobarbital, in addition to the decomposition time of hexobarbital in the liver can also be influenced by a CNS activity. From Table 2 it can be concluded that the test compounds when compared to phenobarbital, have a very small, almost negligible sedative effect
Accordingly, the main biological effect of the compounds is their enzyme inducing activity.
The data of Table 3 unambiguously prove that the compounds according to the invention are far less toxic than phenobarbital.
To sum it up, it can be concluded that after a 24 hours pretreatment the new compounds of the general formula (I) according to the invention show approximately the same enzyme inducing activity as phenobarbital. their main advantage is, however, that after a 1-hour pretreatment they potentiate less or (see the compounds of Examples 3 to 5) not at all the activity of hexobarbital, in other words they are practically devoid of the disadvantageous inhibiting period, which is generally characteristic of the known compounds having enzyme inducing activity. A further advantage of the instant compounds that they show no sedative activity and are far less toxic than phenobarbital.
The compounds of the general formula (I) can be used in the therapy in the form of pharmaceutical compositions containing these active ingredients in admixture with solid or liquid carriers and/or other additives. The compositions may be prepared by methods of pharmaceutical industry known per se.
According to a still further aspect of the present invention we provide pharmaceutical compositions having enzyme inducing activity, which comprises as an active ingredient at least one racemic or optically active compound of the general formula (I) as hereinbefore defined) or a pharmaceutically acceptable acid addition salt thereof, in admixture with inert solid or liquid pharmaceutical carrier.
The pharmaceutical compositions can be formulated in forms suitable for parenteral or enteral administration. As carriers for example water, gelatine, lactose, milk sugar, starch, pectine, magnesium stearate, stearic acid, talc, vegetable oils, such as peanut oil, olive oil, etc. can be used. The compositions may be finished in the form of solid, e.g. tablets, lozenges, dragées, capsules, such as hard gelatine capsule, suppositories, etc. or liquid, e.g. oily or aqueous solutions, suspensions, emulsions, syrups, soft gelatine capsules, injectable oily or aqueous solutions or suspensions, etc.
formulations. The quantity of the solid carrier can be varied within a wide range but preferably is about between 25 mg and 1 g the pharmaceutical compositions optionally can contain also conventional pharmaceutical additives, such as preservatives, stabilizing, wetting, emulsifying agents, salts capable of adjusting the osmotic pressure, buffers, flavouring agents, aroma agents, etc. Optionally further pharmaceutically active compounds can also be present in the formulations.
The pharmaceutical compositions are preferably manufactured in dosage units, suitable for the desired route of administration. The pharmaceutical compositions may be prepared by conventional techniques, which comprise for example screening, admixing, granulating, pressing or dissolving of the components. The compositions obtained can be subjected to further operations conventionally used in the pharmaceutical industry, for example sterilization.
Further details of the present invention are to be found in the following Examples which are, however, by no means intended to limit the scope of protection sought.
Example 1 35,55-1 ,3-dimethyl-5-phenyl-7-chloro-1 3,4, 5-tetrahydro-2H-1 ,4-benzodiazepin-2-one 10 g of 3S-1 ,3-dimethyl-5-phenyl-7-ch loro-l ,3-dihydro-2 H-l ,4-benzodiazepin-2-one are dissolved in 50 ml of acetic acid and to the solution obtained 5 g of sodium borohydride is added portionwise, with stirring, under cooling. Stirring is continued for a further half an hour, whereupon the mixture is neutralized with a 8% aqueous sodium hydrogencarbonate solution under ice cooling. The slowly solidifying product is filtered off, washed with water and is subsequently dried. 9.5 g of the title compound are obtained, melting at 102 to 1 03 OC (after recrystallization from ethanol).
Yield: 95.65%.
[(X] D5=+3 18.9 (c=1.03, chloroform).
Analysis for C17H17N2OCi (molecular weight: 300.79):
calculated: C=67.88%, H=5.69%, N=9.31%
found: C=67.72%, H=7.05%, N=9.49%.
Example 2 3R, SR-i ,3-dimethyl-5-phenyl-7-chloro-1 ,3,4,5-tetrahydro-2H-1 ,4-benzodiazepin-2-one 5 g of 3R-1 ,3-dimethyl-5-phenyl-7-chloro-1 ,3-dihydro-2H-1 ,4-benzodiazepin-2-one are dissolved in 50 ml of acetic acid and to the stirred solution 5 g of zinc powder are added, under cooling with water. Stirring is continued for a further one hour, whereupon the mixture of zinc and zinc salt is filtered off and in the filtrate acetic acid is neutralized by a 8% aqueous sodium hydrogencarbonate solution under ice cooling. The slowly solidifying product is filtered off, washed with water and dried.
4.28 g of the title compound are obtained, melting at 102 to 1 040C (after recrystallization from ethanol).
Yield: 86.29%.
[r11,25=-322.6 (c=1 .015, chloroform), Rf=0.58.
Analysis for C17H17N2OCI (molecular weight: 300.79):
calculated: C=67.88%, H=5.69%, N=9.31%
found: C=67.75%, H=6.95%, N=9.35%.
Example 3 3S,5S-1 ,3-d imethyl-4-carba moyl-5-phenyl-7-chloro-1 3,4, 5-tetrahydro-2H-1 ,4-benzodiazepin- 2-one
1.5 g (4.97 mmoles) of 3S,5S-1 ,3-dimethyl-5-phenyl-7-chloro-1 ,3A,5-tetrnhydrn-2H-1 ,4- benzodiazepin-2-ones are dissolved in a mixture of 10 ml of ethanol and 2 ml of ether and to the solution dry hydrochloric acid gas is introduced. After cooling with ice for 10 minutes the precipitated hydrochloric acid is filtered off and suspended in 10 ml of acetic acid without previous drying. To the suspension 0.5 g (6.1 6 mmoles) of potassium cyanate are added portionwise, under cooling with water and the mixture is stirred for 4 hours. Thereafter 50 g of ice pieces are added to the mixture and it is neutralized with a concentrated aqueous ammonium hydroxide solution.The product, which solidifies upon cooling is filtered off, washed with water and dried. 1.4 g of the title compound are obtained, melting at 208 to 2090C (after recrystallization from ethanol).
Yield: 81.87%.
[!X]D4552.9 (c=0.897, chloroform), Rf=0.06.
Analysis for C18Ha8N302CI (molecular weight: 343.82):
calculated: C=62.88%, H=5.28%, N=12.22% found: C=62.65%, H=6.68%, N=12.07%.
Example 4 3R,5R-1 ,3-d imethyl-4carbamoyl-5-phenyl-7-chloro-1 3,4,5-tetrahydro-2H-1 ,4-benzodiazepin- 2-one
Following the procedure described in Example 3 but starting from 1.5 g of 3R,5R-1 ,3-dimethyl-5- phenyl-7-chloro- 1 ,3,4,5-tetrahydro-2H- 1 ,4-benzodiazepin-2-one, the title compound is obtained, melting at 207 to 2080C.
[sr]DS=+550.4 (c=1 .19, chloroform), Rf=0.06.
Example 5 3RS,5RS-1 ,3-dimethyl-4-carbamoyl-5-phenyl-7-chloro-1 3,4,5-tetra hydro-2H-1 4- benzodiazepin-2-one
Following the procedure described in Example 3 but starting from 1.5 g of 3RS, 5RS-1,3- dimethyl-5-phenyl-7-chloro- 1 ,3,4,5-tetrahydro-2 H- ,4-benzodiazepin-2-one, the title compound is obtained, melting at 241 to 2420C. Ref=0.06.
Claims (2)
1. Optically active or racemic 5-phenyl-1 3,4,5-tetrahydrn-,2H- 1 ,4-benzodiazepin-2-one compounds of general formula (I)
in which the centres of asymmetry in the 3- and 5-positions have the same absolute configuration [wherein
R' represents a hydrogen or halogen atom or a trifluoromethyl or nitro group;
R2 represents a hydrogen atom or an alkyl group having 1 to 6 carbon atoms;
R3 represents a group selected from those groups conventionally attached to the -CH(NH2)-COOH group of the optically active or racemic a-amino-acids;
R4 represents a hydrogen atom or a chlorocarbonyi or carbamoyl group; and
X represents a hydrogen or halogen atom or a trifluoromethyl group; with the proviso that if in the racemic compounds R4 represents a hydrogen atom then R3 represents other than an alkyl group having 1 to 6 carbon atoms] and acid addition salts thereof.
2. Compounds of general formula I as claimed in Claim 1 (wherein R', R2, R4 and X are as defined in Claim 1 and R3 represents a methyl, isopropyl, benzyl, 4-hydroxybenzyl or 3-indolyl-methyl group) and acid addition salts thereof.
2. Compounds of general formula I as claimed in Claim 1 (wherein R:, R2, R4 and X are as defined in Claim 1 and R3 represents a methyl, isopropyl, benzyl-4-hydroxybenzyl or 3-indolyl-methyl group) and acid addition salts thereof.
3. Compounds of general formula I as claimed in Claim 1 (wherein Rr, R2, R4 and X are as defined in Claim 1 and R3 represents an optionally substituted lower alkyl group) and acid addition salts thereof.
4. Compounds of general formula I as claimed in any one of Claims 1 to 3 in the form of pharmaceutically acceptable acid addition salts thereof.
5. 3RS,5RS-1 ,3-dimethyl-4-carbamoyl-5-phenyl-7-chloro- 1 ,3,4,5-tetrahydro-2H- 1,4- benzodiazepin-2-one and pharmaceutically acceptable acid addition salts thereof.
6. 3R, 5 1 ,3-dimethyl-4-carbamoyl-5-phenyl-7-chloro- 1 ,3,4,5-tetrahydro-2H-1 4- benzodiazepin-2-one and pharmaceutically acceptable acid addition salts thereof.
7. 3S,5S-1 ,3-dimethyl-4-carba moyl-5-phenyl-7-ch loro- 1 ,3,4,5-tetra hydro-2 H- 1,4- benzodiazepin-2-one and pharmaceutically acceptable acid addition salts thereof.
8. 3R,5R-1 ,3-dimethyl-5-phenyl-7-chloro-l ,3,4,5-tetrahydro-2H-1 ,4-benzodiazepin-2-one and pharmaceutically acceptable acid addition salts thereof.
9. 3S,5S-1 ,3-dimethyl-5-phenyl-7-chioro-l ,3,4,5-tetrahydro-2H-1 ,4-benzodiazepin-2-one and pharmaceutically acceptable acid addition salts.
1 0. Pharmaceutical compositions having enzyme inducing activity, which comprise as an active ingredient at least one racemic or optically active compound of the general formula (I) (as defined in
Claim 1) or a pharmaceutically acceptable acid addition salt thereof, in admixture with inert solid or liquid pharmaceutical carrier.
11. Pharmaceutical compositions as claimed in Claim 10 containing as active ingredient at least one of the following compounds: 3R5,5R5-1 ,3-dimethyl-4-carbamoyl-5-phenyl-7-chloro-1 ,3,4,5- tetrahydro-2H-l ,4-benzodiazepin-2--one; 3R,5R-1 ,3-dimethyl-4-carbamoyl-5-phenyl-7-chlor 1,3,4,5-tetrahydro-2 1 ,4-benzodiazepin-2-one; and 3S,5S-1 ,3-dimethyl-4-carbamoyl-5-phenyl-7- chloro-1 ,3,4,5-tetrahydro-2H-1 ,4-benzodiazepin-2-one.
12. Pharmaceutical compositions as claimed in either of Claims 10 and 11 in forms suitable for parenteral or enteral administration.
13. Pharmaceutical compositions as claimed in any one of Claims 10 to 12 in dosage unit form.
14. A process for the preparation of racemic or optically active 5-phenyl-1 ,3,4,5-tetrahydro-2H- 1 ,4-benzodiazepin-2-one compounds of general formula (I) (wherein R1, R2, R3 and X are as defined in
Claim 1 and R4 represents a hydrogen atom), in which the centres of asymmetry in the 3- and 5positions have the same absolute configuration, and acid addition salts thereof, which process comprises reducing racemic or optically active 5-phenyl-1 ,3-dihydro-2H-1 ,4-diazepin-2-one compounds of general formula (II)
(wherein R1, R2, R3 and X are as defined above) having a centre of asymmetry in the 3 position and, if desired, converting the product into an acid addition salt thereof.
1 5. A process as claimed in Claim 14 wherein reduction of a 5-phenyl-1 ,3-dihydro-2H-1 ,4- benzodiazepin-2-one of the general formula (II) is carried out with a complex metal hydride, a combination of a metal and an acid or with catalytically activated hydrogen.
1 6. A process as claimed in Claim 1 5 in which sodium borohydride is employed as reducing agent.
17. A process for the preparation of racemic or optically active 5-phenyl-1 ,3,4,5-tetrahydro-2H- 1 ,4-benzodiazepin-2-one compounds of general formula (I) (wherein R1, R2, R3 and X are as defined in
Claim 1 and R4 represents either a carbamoyl group or a chlorocarbonyl group), in which the centres of asymmetry in the 3- and 5-positions have the same absolute configuration, and acid addition salts thereof, which process comprises reacting a racemic or optically active compound of general formula (I) (wherein R4 represents a hydrogen atom and R1, R2, R3 and X are as defined above), or an acid addition salt thereof, with an alkali metal cyanate or with phosgene respectively and, if desired, reacting that product obtained by reaction with phosgene with ammonia.
18. A process as claimed in Claim 1 7 in which the reaction with phosgene is carried out in the presence of an acid binding agent.
1 9. A process as claimed in Claim 17, in which the reaction with ammonia is carried out in a reaction inert organic solvent, optionally in the reaction mixture obtained when preparing the starting compound and ammonia is used as a concentrated aqueous ammonium hydroxide solution or a solution of ammonia in an aliphatic alcohol having 1 to 6 carbon atoms.
20. A process for the preparation of racemic or optically active 5-phenyl-1 ,3,4,5-tetrahydro-2H- 1 ,4-benzodiazepin-2-one compounds of general formula (I) (wherein R', R3, R4 and X are as defined in
Claim 1 and R2 represents an alkyl group having 1 to 6 carbon atoms), in which the centres of asymmetry in the 3- and 5-positions have the same absolute configuration, and acid addition salts thereof, which process comprises reacting a racemic or optically active compound of general formula (I) (wherein R2 represents a hydrogen atom and R1, R3, R4 and X are as defined above), or an acid addition salt thereof, with an alkylating agent.
21. A process as claimed in any one of Claims 1 4 to 20 further comprising the subsequent conversion of the said compound of general formula (I) or acid addition salt thereof to a pharmaceutically acceptable acid addition salt:
22. A process for tne preparation of compounds of general formula I (as defined in Claim 1) and acid addition salts thereof substantially as herein defined.
23. Compounds of general formula I (as defined in Claim 1) and acid addition salts thereof whenever prepared by a process as claimed in any one of Claims 14 to 22.
New Claims filed on 11-11-80.
Superseded Claim 2.
IiBlew or Amended Claim:
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
HU79RI726A HU187262B (en) | 1979-08-16 | 1979-08-16 | Process for preparing new tetrahydro-1,4-benzodiazepin-2-ones |
Publications (2)
Publication Number | Publication Date |
---|---|
GB2056981A true GB2056981A (en) | 1981-03-25 |
GB2056981B GB2056981B (en) | 1983-11-23 |
Family
ID=11001109
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB8026894A Expired GB2056981B (en) | 1979-08-16 | 1980-08-18 | Benzodiazepines |
Country Status (11)
Country | Link |
---|---|
JP (1) | JPS5668675A (en) |
BE (1) | BE884788A (en) |
CA (1) | CA1151166A (en) |
CH (1) | CH647238A5 (en) |
DE (1) | DE3030364A1 (en) |
FR (1) | FR2463131A1 (en) |
GB (1) | GB2056981B (en) |
HU (1) | HU187262B (en) |
IT (1) | IT1147764B (en) |
NL (1) | NL8004564A (en) |
SE (1) | SE441924B (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3921677A1 (en) * | 1989-07-01 | 1991-01-10 | Basf Ag | SEALABLE PLASTIC FILM |
Family Cites Families (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3109843A (en) * | 1963-11-05 | Process for preparing | ||
DE1199776B (en) * | 1959-12-10 | 1965-09-02 | Hoffmann La Roche | Process for the preparation of 5-phenyl-2-oxo-1, 2, 4, 5-tetrahydro-3H-1, 4-benzodiazepine derivatives |
NL131197C (en) * | 1963-06-28 | |||
US3522289A (en) * | 1964-06-15 | 1970-07-28 | Hoffmann La Roche | Process for preparing a 2(1-(2-amino-5-phenyl) - 1 - phenylmethylamino)acetic acid derivative |
CH510678A (en) * | 1966-12-03 | 1971-07-31 | Richter Gedeon Vegyeszet | 1 3 4 5-tetrahydro-2h-1 4-benzodiazepine 2-one derivs |
AT281035B (en) * | 1966-12-28 | 1970-05-11 | Richter Gedeon Vegyeszet | Process for the preparation of 1,3-dihydro-2H-1,4-benzodiazepine derivatives and their acid addition salts |
CH558370A (en) * | 1970-07-30 | 1975-01-31 | Richter Gedeon Vegyeszet | 1,3,4,5-tetrahydro 2h 1,4-benzodiazepin 2-ones - by reductive cyclization of n-protected n-glycylamido-benzophenones |
CH557358A (en) * | 1970-07-30 | 1974-12-31 | Richter Gedeon Vegyeszet | Optically active 1,4-benzodiazepine 2-ones - tranquilizers and muscle relaxants |
DE2204484B2 (en) * | 1971-02-09 | 1978-04-13 | Sumitomo Chemical Co | Process for the preparation of 5-phenyl-23-dihydro-IH-1,4-benzodiazepines |
JPS4825199A (en) * | 1971-08-03 | 1973-04-02 | ||
US3864330A (en) * | 1971-08-31 | 1975-02-04 | Sumitomo Chemical Co | Benzodiazepine derivatives and production thereof |
HU171033B (en) * | 1974-05-29 | 1977-10-28 | Richter Gedeon Vegyeszet | Process for producing benzodiazepine derivatives |
HU170623B (en) * | 1974-06-25 | 1977-07-28 |
-
1979
- 1979-08-16 HU HU79RI726A patent/HU187262B/en not_active IP Right Cessation
-
1980
- 1980-08-04 IT IT68248/80A patent/IT1147764B/en active
- 1980-08-11 DE DE19803030364 patent/DE3030364A1/en not_active Ceased
- 1980-08-12 NL NL8004564A patent/NL8004564A/en not_active Application Discontinuation
- 1980-08-13 FR FR8017856A patent/FR2463131A1/en active Granted
- 1980-08-14 BE BE0/201759A patent/BE884788A/en not_active IP Right Cessation
- 1980-08-15 SE SE8005779A patent/SE441924B/en not_active IP Right Cessation
- 1980-08-15 CA CA000358371A patent/CA1151166A/en not_active Expired
- 1980-08-15 CH CH6185/80A patent/CH647238A5/en not_active IP Right Cessation
- 1980-08-16 JP JP11231480A patent/JPS5668675A/en active Granted
- 1980-08-18 GB GB8026894A patent/GB2056981B/en not_active Expired
Also Published As
Publication number | Publication date |
---|---|
BE884788A (en) | 1981-02-16 |
JPH0325425B2 (en) | 1991-04-05 |
IT8068248A0 (en) | 1980-08-04 |
CH647238A5 (en) | 1985-01-15 |
SE441924B (en) | 1985-11-18 |
HU187262B (en) | 1985-12-28 |
GB2056981B (en) | 1983-11-23 |
CA1151166A (en) | 1983-08-02 |
DE3030364A1 (en) | 1981-03-26 |
JPS5668675A (en) | 1981-06-09 |
FR2463131A1 (en) | 1981-02-20 |
FR2463131B1 (en) | 1985-04-26 |
SE8005779L (en) | 1981-02-17 |
IT1147764B (en) | 1986-11-26 |
NL8004564A (en) | 1981-02-18 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PCNP | Patent ceased through non-payment of renewal fee |
Effective date: 19930818 |