GB1562036A - Chromatographic columns - Google Patents

Chromatographic columns Download PDF

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Publication number
GB1562036A
GB1562036A GB13240/77A GB1324077A GB1562036A GB 1562036 A GB1562036 A GB 1562036A GB 13240/77 A GB13240/77 A GB 13240/77A GB 1324077 A GB1324077 A GB 1324077A GB 1562036 A GB1562036 A GB 1562036A
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United Kingdom
Prior art keywords
sleeve
column
layer
tube
fusible
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB13240/77A
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Cytiva Sweden AB
Original Assignee
Pharmacia Fine Chemicals AB
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Filing date
Publication date
Application filed by Pharmacia Fine Chemicals AB filed Critical Pharmacia Fine Chemicals AB
Publication of GB1562036A publication Critical patent/GB1562036A/en
Expired legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/22—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C65/00—Joining or sealing of preformed parts, e.g. welding of plastics materials; Apparatus therefor
    • B29C65/66—Joining or sealing of preformed parts, e.g. welding of plastics materials; Apparatus therefor by liberation of internal stresses, e.g. shrinking of one of the parts to be joined
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C66/00—General aspects of processes or apparatus for joining preformed parts
    • B29C66/01—General aspects dealing with the joint area or with the area to be joined
    • B29C66/05—Particular design of joint configurations
    • B29C66/10—Particular design of joint configurations particular design of the joint cross-sections
    • B29C66/11—Joint cross-sections comprising a single joint-segment, i.e. one of the parts to be joined comprising a single joint-segment in the joint cross-section
    • B29C66/112—Single lapped joints
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C66/00—General aspects of processes or apparatus for joining preformed parts
    • B29C66/01—General aspects dealing with the joint area or with the area to be joined
    • B29C66/05—Particular design of joint configurations
    • B29C66/10—Particular design of joint configurations particular design of the joint cross-sections
    • B29C66/12—Joint cross-sections combining only two joint-segments; Tongue and groove joints; Tenon and mortise joints; Stepped joint cross-sections
    • B29C66/122—Joint cross-sections combining only two joint-segments, i.e. one of the parts to be joined comprising only two joint-segments in the joint cross-section
    • B29C66/1222—Joint cross-sections combining only two joint-segments, i.e. one of the parts to be joined comprising only two joint-segments in the joint cross-section comprising at least a lapped joint-segment
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C66/00—General aspects of processes or apparatus for joining preformed parts
    • B29C66/01—General aspects dealing with the joint area or with the area to be joined
    • B29C66/05—Particular design of joint configurations
    • B29C66/10—Particular design of joint configurations particular design of the joint cross-sections
    • B29C66/12—Joint cross-sections combining only two joint-segments; Tongue and groove joints; Tenon and mortise joints; Stepped joint cross-sections
    • B29C66/122—Joint cross-sections combining only two joint-segments, i.e. one of the parts to be joined comprising only two joint-segments in the joint cross-section
    • B29C66/1224—Joint cross-sections combining only two joint-segments, i.e. one of the parts to be joined comprising only two joint-segments in the joint cross-section comprising at least a butt joint-segment
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C66/00—General aspects of processes or apparatus for joining preformed parts
    • B29C66/01—General aspects dealing with the joint area or with the area to be joined
    • B29C66/05—Particular design of joint configurations
    • B29C66/10—Particular design of joint configurations particular design of the joint cross-sections
    • B29C66/13—Single flanged joints; Fin-type joints; Single hem joints; Edge joints; Interpenetrating fingered joints; Other specific particular designs of joint cross-sections not provided for in groups B29C66/11 - B29C66/12
    • B29C66/131—Single flanged joints, i.e. one of the parts to be joined being rigid and flanged in the joint area
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C66/00—General aspects of processes or apparatus for joining preformed parts
    • B29C66/50—General aspects of joining tubular articles; General aspects of joining long products, i.e. bars or profiled elements; General aspects of joining single elements to tubular articles, hollow articles or bars; General aspects of joining several hollow-preforms to form hollow or tubular articles
    • B29C66/51—Joining tubular articles, profiled elements or bars; Joining single elements to tubular articles, hollow articles or bars; Joining several hollow-preforms to form hollow or tubular articles
    • B29C66/52—Joining tubular articles, bars or profiled elements
    • B29C66/522—Joining tubular articles
    • B29C66/5224—Joining tubular articles for forming fork-shaped connections, e.g. for making Y-shaped pieces
    • B29C66/52241—Joining tubular articles for forming fork-shaped connections, e.g. for making Y-shaped pieces with two right angles, e.g. for making T-shaped pieces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C66/00—General aspects of processes or apparatus for joining preformed parts
    • B29C66/50—General aspects of joining tubular articles; General aspects of joining long products, i.e. bars or profiled elements; General aspects of joining single elements to tubular articles, hollow articles or bars; General aspects of joining several hollow-preforms to form hollow or tubular articles
    • B29C66/51—Joining tubular articles, profiled elements or bars; Joining single elements to tubular articles, hollow articles or bars; Joining several hollow-preforms to form hollow or tubular articles
    • B29C66/52—Joining tubular articles, bars or profiled elements
    • B29C66/522—Joining tubular articles
    • B29C66/5229—Joining tubular articles involving the use of a socket
    • B29C66/52291—Joining tubular articles involving the use of a socket said socket comprising a stop
    • B29C66/52293—Joining tubular articles involving the use of a socket said socket comprising a stop said stop being external
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C66/00—General aspects of processes or apparatus for joining preformed parts
    • B29C66/50—General aspects of joining tubular articles; General aspects of joining long products, i.e. bars or profiled elements; General aspects of joining single elements to tubular articles, hollow articles or bars; General aspects of joining several hollow-preforms to form hollow or tubular articles
    • B29C66/51—Joining tubular articles, profiled elements or bars; Joining single elements to tubular articles, hollow articles or bars; Joining several hollow-preforms to form hollow or tubular articles
    • B29C66/52—Joining tubular articles, bars or profiled elements
    • B29C66/522—Joining tubular articles
    • B29C66/5229—Joining tubular articles involving the use of a socket
    • B29C66/52296—Joining tubular articles involving the use of a socket said socket comprising sealing elements, e.g. gaskets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C66/00—General aspects of processes or apparatus for joining preformed parts
    • B29C66/50—General aspects of joining tubular articles; General aspects of joining long products, i.e. bars or profiled elements; General aspects of joining single elements to tubular articles, hollow articles or bars; General aspects of joining several hollow-preforms to form hollow or tubular articles
    • B29C66/51—Joining tubular articles, profiled elements or bars; Joining single elements to tubular articles, hollow articles or bars; Joining several hollow-preforms to form hollow or tubular articles
    • B29C66/53—Joining single elements to tubular articles, hollow articles or bars
    • B29C66/534—Joining single elements to open ends of tubular or hollow articles or to the ends of bars
    • B29C66/5344—Joining single elements to open ends of tubular or hollow articles or to the ends of bars said single elements being substantially annular, i.e. of finite length, e.g. joining flanges to tube ends
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C66/00—General aspects of processes or apparatus for joining preformed parts
    • B29C66/70—General aspects of processes or apparatus for joining preformed parts characterised by the composition, physical properties or the structure of the material of the parts to be joined; Joining with non-plastics material
    • B29C66/72—General aspects of processes or apparatus for joining preformed parts characterised by the composition, physical properties or the structure of the material of the parts to be joined; Joining with non-plastics material characterised by the structure of the material of the parts to be joined
    • B29C66/723—General aspects of processes or apparatus for joining preformed parts characterised by the composition, physical properties or the structure of the material of the parts to be joined; Joining with non-plastics material characterised by the structure of the material of the parts to be joined being multi-layered
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C66/00—General aspects of processes or apparatus for joining preformed parts
    • B29C66/70—General aspects of processes or apparatus for joining preformed parts characterised by the composition, physical properties or the structure of the material of the parts to be joined; Joining with non-plastics material
    • B29C66/73—General aspects of processes or apparatus for joining preformed parts characterised by the composition, physical properties or the structure of the material of the parts to be joined; Joining with non-plastics material characterised by the intensive physical properties of the material of the parts to be joined, by the optical properties of the material of the parts to be joined, by the extensive physical properties of the parts to be joined, by the state of the material of the parts to be joined or by the material of the parts to be joined being a thermoplastic or a thermoset
    • B29C66/737—General aspects of processes or apparatus for joining preformed parts characterised by the composition, physical properties or the structure of the material of the parts to be joined; Joining with non-plastics material characterised by the intensive physical properties of the material of the parts to be joined, by the optical properties of the material of the parts to be joined, by the extensive physical properties of the parts to be joined, by the state of the material of the parts to be joined or by the material of the parts to be joined being a thermoplastic or a thermoset characterised by the state of the material of the parts to be joined
    • B29C66/7371—General aspects of processes or apparatus for joining preformed parts characterised by the composition, physical properties or the structure of the material of the parts to be joined; Joining with non-plastics material characterised by the intensive physical properties of the material of the parts to be joined, by the optical properties of the material of the parts to be joined, by the extensive physical properties of the parts to be joined, by the state of the material of the parts to be joined or by the material of the parts to be joined being a thermoplastic or a thermoset characterised by the state of the material of the parts to be joined oriented or heat-shrinkable
    • B29C66/73715—General aspects of processes or apparatus for joining preformed parts characterised by the composition, physical properties or the structure of the material of the parts to be joined; Joining with non-plastics material characterised by the intensive physical properties of the material of the parts to be joined, by the optical properties of the material of the parts to be joined, by the extensive physical properties of the parts to be joined, by the state of the material of the parts to be joined or by the material of the parts to be joined being a thermoplastic or a thermoset characterised by the state of the material of the parts to be joined oriented or heat-shrinkable heat-shrinkable
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6004—Construction of the column end pieces
    • G01N30/6021—Adjustable pistons
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C66/00—General aspects of processes or apparatus for joining preformed parts
    • B29C66/70—General aspects of processes or apparatus for joining preformed parts characterised by the composition, physical properties or the structure of the material of the parts to be joined; Joining with non-plastics material
    • B29C66/71—General aspects of processes or apparatus for joining preformed parts characterised by the composition, physical properties or the structure of the material of the parts to be joined; Joining with non-plastics material characterised by the composition of the plastics material of the parts to be joined
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/30—Control of physical parameters of the fluid carrier of temperature
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6004—Construction of the column end pieces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6004—Construction of the column end pieces
    • G01N30/6026—Fluid seals

Description

(54) IMPROVEMENTS IN OR RELATING TO CHROMATOGRAPHIC COLUMNS (71) We, PHARMACIA FINE CHEMICALS AB, of Box 175, S-751 of Uppsala 1, Sweden, do hereby declare the invention, for which we pray that a patent may be granted to us, and the method by which it is to be performed, to be particularly described in and by the following statement: The invention refers to so called chromatographic columns to be used preferably in laboratories, the invention also comprising a method of assembling such a column.
Chromatographic columns comprise an inner tube, normally of glass, the so called column tube, in which the separation is performed and at the ends some kind of flanges for mounting means supplying liquids to and withdrawing them from the column tube. These flanges are also used for mounting and sealing a cooler casing, if used, around the column tube enabling temperature controlling fluids to be circu lated through the interspace.
Previously used columns have been comparatively expensive and difficult to manufacture because it has been necessary to shape the column tube in a specific way with widened ends necessitating specific manufacture of glass tubes having such terminal flanges for this. It is the purpose of the present invention to eliminate this drawback by providing a column and a method of assembling such a column where specifically manufactured glass tubes are no longer required and ordinary straight standard tubes can be used.
When trying to realize this purpose by attempting to use ordinary straight stan dard tubes in place of the previously used special tubes having flanged ends it is, however, necessary to solve the problem of securely attaching the terminal flanges at the glass tube paying consideration to the fact that the joint during use of the column is exposed to several different types of solvents, this means that most types of cement are unsuitable, in particu lar as the end flanges often have to be manufactured of plastic materials having unsatisfactory cementing properties.
There is the alternative possibility of providing the glass tube with threads and to attach the terminal flanges by screwing but, while this is a possible solution, it requires a complicated working of the glass tube and forbids direct use of straight standard tubes.
According to one aspect of the present invention, there is provided a chromatographic column comprising a column tube and a terminal flange attached to the tube by a sleeve of two layers of material, which sleeve has been subjected to a heat treatment, an inner layer of material which sleeve is of a plastics material, which plastics material was, prior to the heat treatment, fusible, and an outer later of material of which sleeve is of a second plastics material, which second plastics material was, prior to the heat treatment, heat shrinkable, and which heat treatment causes the fusible plastics layer of material to fuse and the heat shrinkable plastics layer of material to shrink.
According to a second aspect of the present invention, there is provided a method of attaching a tube of a chromatographic column to a terminal flange, which method comprises using a sleeve of two layers of material, an inner layer of material of which sleeve is of fusible plastics material, and an outer layer of material of which sleeve is of heat shrinkable plastics material, and subjecting the sleeve to a heat treatment causing the fusible plastics layer of material to fuse and the heat shrinkable plastics layer of material to shrink.
In order that the present invention may be more readily understood, and to show how the same may be put into effect, reference will now be made, by way of example, to the accompanying drawing, which shows the upper half of a column for liquid chromatography shown in section and with certain parts shown in elevation for the sake of clarity.
The separation column shown in the drawing has a mantle tube 6 within which a column tube 2 extends. A temperature controlling medium is caused to circulate in the interspace between the mantle tube 6 and the column tube 2, this medium being supplied and discharged respectively by means of conduits not shown and connected to nipples one of which only is shown at 7 in the drawing. Connecting nipple 7 forms a part of a terminal sleeve 10 which in its mounted position extends slightly into the mantle tube 6, a sealing ring 13 sealing the terminal sleeve in relation to the mantle tube. The upper part of the terminal sleeve 10 has an outwardly threaded portion 9 adapted to cooperate with an upper terminal piece 8 provided with a corresponding inner thread.
Moreover the terminal piece 8 is provided with a radially extending threaded bore through which a mounting screw 4 having a control knob 5 extends. Both the column tube 2 and the mantle tube 6 are straight glass tubes and no specifically blown glass tube constructions are required in connection with the present invention. The terminal sleeve 10 and the terminal piece 8 are made of workable plastic material and, accordingly, the threaded portions are easily manufactured.
At its end portions, column tube 2 is provided with end flanges, the upper one of which is shown at 11 in the drawing.
This end flange 11 is attached to the column tube 2 in a way to be described later on and comprises sealing means such as the O-ring 12 for sealing between end flange 11 and end sleeve 10, and mounting means 18, such as a locating ring, key means or the like adapted to retain the column tube 2 in proper position in the chromatographic column. End flange 11 as well as the rest of the mounting means may advantageously be made of plastic materials which are resistive to solvents, for example a polytetrafluoroethylene material.
It will be appreciated that problems are encountered if a flange of such material as polytetrafluoroethylene is to be attached to a glass tube, the present invention, however, offering a solution of this problem.
In accordance with the present invention the straight column tube 2 of glass is attached to the end flange of solvent resis tive, workable plastic material in the following way. The lower portion or extension 14 of the terminal flange 11 which may be terminated by a bead 15 is outwardly threaded onto an outer portion of the column tube 2. A layer of fusible plastic is applied over a portion of the extension 14 of the end flange 11 and a portion 17 of the column tube 2 consisting of glass. This layer of fusible plastic may be in the form of a fusible hose or fusible tape. A hose of shrinking plastic is outwardly applied onto said fusible plastic layer. As a rule the hose material is a polyolefine (polyethylene), a polyvinylchloride (PVC) or fluoroplastics such as polyvinylidene fluoride, polytetrafluoroethylene (PTFE), fluoroethylene polymer (FEP). Shrinking plastics are manufactured by spray forming to the shape the product is to have in its shrunk condition. Thereafter the product is irradiated with high-energy electrons (beta radiation) causing cross-links to be formed between the molecule chains. Subsequently these will act as "elastic memories". When the products are heat treated immediately above the normal melting point of the plastic it will not melt but rather become resilient. When the hose simultaneously is exposed to internal overpressure the hose will be enlarged, this enlargement being retained if the overpressure is maintained during cooling. When again heated above the normal melting point the plastic will tend to resume its original shape.
In order to obtain improved sealing and better adhesion a fusible hose may be inserted into the shrinking hose. The fusible plastic layer and the shrinking plastic hose may be combined into a hose structure, the fusible hose comprising a nonirradiated polyolefine if the shrinking hose is of olefine type. It is also possible to combine a fusible hose of FEP with a shrinking hose of irradiated PTFE. When combining fusible plastic and shrinking plastic care should be taken that the fusible plastic fuses at the temperature at which the shrinking plastic shrinks. A ready-made shrinking hose of irradiated polyolefine combined with an internal fusing layer of non-irradiated polyolefine is available on the market. It is also possible to buy shrinking hose of PTFE enclosing an untreated inner hose of FEP. When the combination hose or fusible plastic layer and shrinking plastic hose have been applied and heat treated at a temperature immediately above the fusing and shrinking temperatures the fusing layer will fuse and adheres to the base while the shrinking hose will shrink and perform a radially directed force against the fusible layer while simultaneously absorbing axially directed forces between the terminal flange and the column tube. It has been experimentally shown that the joint has little tendency towards fatigue after extended use.
The assembly of the column according to the invention is performed in the following way. The terminal flange 11 is provided with the two-layer hose as at 16, said hose comprising said fusible plastic layer and said shrinking plastic hose. The unit so formed is threaded onto the column tube 2 in the correct position as indicated at 17. By the application of heat the fusible plastic layer is caused to fuse and the shrinking plastic hose is caused to contract so that a safe and solid joint is obtained. The column tube 2 provided in this way with the terminal flange is thereafter assembled with the terminal sleeve 10 and is held in place by the locking ring 18. Subsequently the unit is combined with the mantle tube 6. Thereafter the upper terminal piece 8 is screwed-on, the flow adaptor 1 is inserted and the mounting screw 4 is so tightened that it comes into contact with the flow adapter. Thereafter the separation column is ready for use.
According to the invention there is obtained a column manufactured of straight tubes only, thus eliminating the expensive manufacture of special glass required in prior-art construction. A safe assembly is obtained which resists stresses incident to the filling and use of the column. By using workable, easily manufactured and cheap plastic components the construction is rendered cheaper and the assembly is essentially facilitated in relation to prior-art devices.
The expert of the field will appreciate that variations and modifications may be made within the frame of the invention as defined in the present claims without departure from the basic idea of the invention. Certain presently preferred materials have been mentioned above, it being obvious that other types of material having similar properties may be used provided they yield the same function as described above.
WHAT WE CLAIM IS: 1. A chromatographic column comprising a column tube and a terminal flange attached to the tube by a sleeve of two layers of material, which sleeve has been subjected to a heat treatment, an inner layer of material of which sleeve is of a plastics material, which plastics material was, prior to the heat treatment, fusible, and an outer layer of material of which sleeve is of a second plastics material, which second plastics material was, prior to the heat treatment, heat shrinkable, and which heat treatment causes the fusible plastics layer of material to fuse and the heat shrinkable plastics layer of material to shrink.
2. A method of attaching a tube of a chromatographic column to a terminal flange, which method comprises using a sleeve of two layers of material, an inner layer of material of which sleeve is of fusible plastics material, and an outer layer of material of which sleeve is of heat shrinkable plastics material, and subjecting the sleeve to a heat treatment causing the fusible plastics layer of material to fuse and the heat shrinkable plastics layer of material to shrink.
3. A chromatographic column, substantially as hereinbefore described with reference to, and as shown in, the accompanying drawing.
4. A method of attaching a terminal flange to a chromatographic column tube, substantially as hereinbefore described with reference to the accompanying drawing.
**WARNING** end of DESC field may overlap start of CLMS **.

Claims (4)

**WARNING** start of CLMS field may overlap end of DESC **. flange and the column tube. It has been experimentally shown that the joint has little tendency towards fatigue after extended use. The assembly of the column according to the invention is performed in the following way. The terminal flange 11 is provided with the two-layer hose as at 16, said hose comprising said fusible plastic layer and said shrinking plastic hose. The unit so formed is threaded onto the column tube 2 in the correct position as indicated at 17. By the application of heat the fusible plastic layer is caused to fuse and the shrinking plastic hose is caused to contract so that a safe and solid joint is obtained. The column tube 2 provided in this way with the terminal flange is thereafter assembled with the terminal sleeve 10 and is held in place by the locking ring 18. Subsequently the unit is combined with the mantle tube 6. Thereafter the upper terminal piece 8 is screwed-on, the flow adaptor 1 is inserted and the mounting screw 4 is so tightened that it comes into contact with the flow adapter. Thereafter the separation column is ready for use. According to the invention there is obtained a column manufactured of straight tubes only, thus eliminating the expensive manufacture of special glass required in prior-art construction. A safe assembly is obtained which resists stresses incident to the filling and use of the column. By using workable, easily manufactured and cheap plastic components the construction is rendered cheaper and the assembly is essentially facilitated in relation to prior-art devices. The expert of the field will appreciate that variations and modifications may be made within the frame of the invention as defined in the present claims without departure from the basic idea of the invention. Certain presently preferred materials have been mentioned above, it being obvious that other types of material having similar properties may be used provided they yield the same function as described above. WHAT WE CLAIM IS:
1. A chromatographic column comprising a column tube and a terminal flange attached to the tube by a sleeve of two layers of material, which sleeve has been subjected to a heat treatment, an inner layer of material of which sleeve is of a plastics material, which plastics material was, prior to the heat treatment, fusible, and an outer layer of material of which sleeve is of a second plastics material, which second plastics material was, prior to the heat treatment, heat shrinkable, and which heat treatment causes the fusible plastics layer of material to fuse and the heat shrinkable plastics layer of material to shrink.
2. A method of attaching a tube of a chromatographic column to a terminal flange, which method comprises using a sleeve of two layers of material, an inner layer of material of which sleeve is of fusible plastics material, and an outer layer of material of which sleeve is of heat shrinkable plastics material, and subjecting the sleeve to a heat treatment causing the fusible plastics layer of material to fuse and the heat shrinkable plastics layer of material to shrink.
3. A chromatographic column, substantially as hereinbefore described with reference to, and as shown in, the accompanying drawing.
4. A method of attaching a terminal flange to a chromatographic column tube, substantially as hereinbefore described with reference to the accompanying drawing.
GB13240/77A 1976-04-14 1977-03-29 Chromatographic columns Expired GB1562036A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE7604445A SE398396B (en) 1976-04-14 1976-04-14 SEPARATION COLUMN AND WAY TO COMPOSE DYLIK

Publications (1)

Publication Number Publication Date
GB1562036A true GB1562036A (en) 1980-03-05

Family

ID=20327602

Family Applications (1)

Application Number Title Priority Date Filing Date
GB13240/77A Expired GB1562036A (en) 1976-04-14 1977-03-29 Chromatographic columns

Country Status (4)

Country Link
JP (1) JPS52127296A (en)
DE (1) DE2715389A1 (en)
GB (1) GB1562036A (en)
SE (1) SE398396B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE29512372U1 (en) * 1995-08-01 1995-10-05 Hewlett Packard Gmbh Separation column for chromatography

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE8800644L (en) * 1988-02-25 1989-08-26 Pharmacia Ab DEVICE TO ENABLE DIFFERENT GELBED HEIGHTS IN A CHROMATOGRAPHIC SEPARATION COLUMN

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE29512372U1 (en) * 1995-08-01 1995-10-05 Hewlett Packard Gmbh Separation column for chromatography

Also Published As

Publication number Publication date
SE398396B (en) 1977-12-19
SE7604445L (en) 1977-10-15
JPS52127296A (en) 1977-10-25
DE2715389A1 (en) 1977-11-03

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PCNP Patent ceased through non-payment of renewal fee