GB1529097A - Potentiated human growth hormone and process for producing same - Google Patents
Potentiated human growth hormone and process for producing sameInfo
- Publication number
- GB1529097A GB1529097A GB36893/76A GB3689376A GB1529097A GB 1529097 A GB1529097 A GB 1529097A GB 36893/76 A GB36893/76 A GB 36893/76A GB 3689376 A GB3689376 A GB 3689376A GB 1529097 A GB1529097 A GB 1529097A
- Authority
- GB
- United Kingdom
- Prior art keywords
- hgh
- growth hormone
- human growth
- modified
- dialysed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000002265 Human Growth Hormone Human genes 0.000 title abstract 8
- 108010000521 Human Growth Hormone Proteins 0.000 title abstract 8
- 239000000854 Human Growth Hormone Substances 0.000 title abstract 8
- 238000000034 method Methods 0.000 title abstract 2
- 108091005804 Peptidases Proteins 0.000 abstract 2
- 102000035195 Peptidases Human genes 0.000 abstract 2
- 235000019833 protease Nutrition 0.000 abstract 2
- GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 abstract 1
- 108090000787 Subtilisin Proteins 0.000 abstract 1
- 125000000539 amino acid group Chemical group 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 abstract 1
- MUCZHBLJLSDCSD-UHFFFAOYSA-N diisopropyl fluorophosphate Chemical compound CC(C)OP(F)(=O)OC(C)C MUCZHBLJLSDCSD-UHFFFAOYSA-N 0.000 abstract 1
- 239000001488 sodium phosphate Substances 0.000 abstract 1
- 229910000162 sodium phosphate Inorganic materials 0.000 abstract 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Endocrinology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
1529097 Modified hGH SCRIPPS CLINIC & RESEARCH FOUNDATION 6 Sept 1976 [8 Sept 1975] 36893/76 Heading C3H A method of producing modified human growth hormone (hGH) of increased biological potency comprises digesting hGH with a proteinase to remove amino acid residues 137 to at most 149 and to at least 147 inclusive, and recovering the modified hGH. Preferably the proteinase is of bacterial origin, e.g. subtilisin. In an example hGH is digested in a solution of sodium phosphate containing subtilism. The reaction is terminated by addition of diisopropylfluorphosphate and the solution is then dialysed. The dialysed digest is then fractionated on DEAE-cellulose and three hGH fractions result.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US61092575A | 1975-09-08 | 1975-09-08 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB1529097A true GB1529097A (en) | 1978-10-18 |
Family
ID=24446955
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB36893/76A Expired GB1529097A (en) | 1975-09-08 | 1976-09-06 | Potentiated human growth hormone and process for producing same |
Country Status (4)
Country | Link |
---|---|
DE (1) | DE2640431A1 (en) |
GB (1) | GB1529097A (en) |
IT (1) | IT1066215B (en) |
SE (1) | SE7609861L (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5079345A (en) * | 1988-08-19 | 1992-01-07 | Eli Lilly And Company | Proteins having growth hormone anabolic properties with reduced effect on carbohydrate metabolism |
-
1976
- 1976-08-30 IT IT51086/76A patent/IT1066215B/en active
- 1976-09-06 GB GB36893/76A patent/GB1529097A/en not_active Expired
- 1976-09-07 SE SE7609861A patent/SE7609861L/en unknown
- 1976-09-08 DE DE19762640431 patent/DE2640431A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
SE7609861L (en) | 1977-03-09 |
DE2640431A1 (en) | 1977-03-17 |
IT1066215B (en) | 1985-03-04 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PS | Patent sealed | ||
PCNP | Patent ceased through non-payment of renewal fee |