GB1528011A - Continuous culture of micro-organisms - Google Patents

Continuous culture of micro-organisms

Info

Publication number
GB1528011A
GB1528011A GB5747/75A GB574775A GB1528011A GB 1528011 A GB1528011 A GB 1528011A GB 5747/75 A GB5747/75 A GB 5747/75A GB 574775 A GB574775 A GB 574775A GB 1528011 A GB1528011 A GB 1528011A
Authority
GB
United Kingdom
Prior art keywords
organisms
micro
hydrocarbon
methanol
growth medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB5747/75A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shell Internationale Research Maatschappij BV
Original Assignee
Shell Internationale Research Maatschappij BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shell Internationale Research Maatschappij BV filed Critical Shell Internationale Research Maatschappij BV
Priority to GB5747/75A priority Critical patent/GB1528011A/en
Priority to CA243,112A priority patent/CA1059049A/en
Priority to BE1007186A priority patent/BE838312A/en
Priority to IT19993/76A priority patent/IT1055875B/en
Priority to NO760414A priority patent/NO760414L/no
Priority to IE251/76A priority patent/IE42634B1/en
Priority to DE19762604993 priority patent/DE2604993A1/en
Priority to FR7603439A priority patent/FR2300803A1/en
Priority to NL7601314A priority patent/NL7601314A/en
Publication of GB1528011A publication Critical patent/GB1528011A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/32—Processes using, or culture media containing, lower alkanols, i.e. C1 to C6
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/26—Processes using, or culture media containing, hydrocarbons

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

1528011 Continuous cultivation of bacteria &c on methanol &c SHELL INTERNATIONALE RESEARCH MAATSCHAPPIJ BV 9 Feb 1976 [11 Feb 1975] 5747/75 Heading C6F In a process for the production of microorganisms involving continuously cultivating one or more hydrocarbon or oxygenated hydrocarbon utilizing micro-organisms under aerobic conditions in a liquid growth medium comprising assimilable sources of nitrogen and essential mineral salts in the presence of a hydrocarbon or oxygenated hydrocarbon, withdrawing from the growing culture, liquid growth medium containing micron organisms, separating micro-organisms from the withdrawn liquid growth medium and recycling at least part of the withdrawn liquid growth medium to the growing culture, the withdrawn liquid growth medium is chilled to a temperature in the range of -5‹ to +20‹C prior to, or during, the separation of the micro-organisms. It is stated that any buildup of growth-inhibiting material during the separation stage is avoided and contamination of the separation and recycle lines by undesirable anaerobic micro-organisms is discouraged as a result of the chilling. The hydrocarbon or oxygenated hydrocarbon may for example be natural gas, methane or methanol. The Examples relate to the continuous cultivation of a mixed culture of microorganisms comprising a methanol-utilizing bacterium and four non-methanol utilizing bacteria in methanolic culture medium, with recycle of the culture medium and recovery of the micro-organisms produced. The cultivation temperature was 40‹ or 42‹C and the chilling temperature 0‹C. Growth inhibition was encountered when recycling took place without the chilling according to the invention.
GB5747/75A 1975-02-11 1975-02-11 Continuous culture of micro-organisms Expired GB1528011A (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
GB5747/75A GB1528011A (en) 1975-02-11 1975-02-11 Continuous culture of micro-organisms
CA243,112A CA1059049A (en) 1975-02-11 1976-01-07 Cultivation of micro-organisms on hydrocarbon medium
BE1007186A BE838312A (en) 1975-02-11 1976-02-06 PROCESS FOR PREPARING MICRO-ORGANISMS
IT19993/76A IT1055875B (en) 1975-02-11 1976-02-09 PRACESS FOR THE PRODUCTION OF MICROORGANISMS
NO760414A NO760414L (en) 1975-02-11 1976-02-09
IE251/76A IE42634B1 (en) 1975-02-11 1976-02-09 Improvements in and relating to the continuous culture of micro-organisms
DE19762604993 DE2604993A1 (en) 1975-02-11 1976-02-09 METHOD OF BREEDING MICRO-ORGANISMS
FR7603439A FR2300803A1 (en) 1975-02-11 1976-02-09 PROCESS FOR THE PRODUCTION OF MICRO-ORGANISMS
NL7601314A NL7601314A (en) 1975-02-11 1976-02-10 PROCESS FOR PREPARING MICRO-ORGANISMS.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB5747/75A GB1528011A (en) 1975-02-11 1975-02-11 Continuous culture of micro-organisms

Publications (1)

Publication Number Publication Date
GB1528011A true GB1528011A (en) 1978-10-11

Family

ID=9801902

Family Applications (1)

Application Number Title Priority Date Filing Date
GB5747/75A Expired GB1528011A (en) 1975-02-11 1975-02-11 Continuous culture of micro-organisms

Country Status (9)

Country Link
BE (1) BE838312A (en)
CA (1) CA1059049A (en)
DE (1) DE2604993A1 (en)
FR (1) FR2300803A1 (en)
GB (1) GB1528011A (en)
IE (1) IE42634B1 (en)
IT (1) IT1055875B (en)
NL (1) NL7601314A (en)
NO (1) NO760414L (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2492403A1 (en) * 1980-10-20 1982-04-23 Bel Fromageries PROCESS FOR THE CULTURE OF MICROORGANISMS, PARTICULARLY YEAST, ON WHEY WITH A ASSOCIATION OF WELL-SELECTED STRAINS AND STRAIN-SPECIFIC MUTANTS

Also Published As

Publication number Publication date
CA1059049A (en) 1979-07-24
IT1055875B (en) 1982-01-11
IE42634L (en) 1976-08-11
BE838312A (en) 1976-08-06
NL7601314A (en) 1976-08-13
NO760414L (en) 1976-08-12
FR2300803B1 (en) 1978-11-10
FR2300803A1 (en) 1976-09-10
DE2604993A1 (en) 1976-08-19
IE42634B1 (en) 1980-09-24

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PCNP Patent ceased through non-payment of renewal fee