GB1525535A - Process for culturing cells - Google Patents

Process for culturing cells

Info

Publication number
GB1525535A
GB1525535A GB13442/76A GB1344276A GB1525535A GB 1525535 A GB1525535 A GB 1525535A GB 13442/76 A GB13442/76 A GB 13442/76A GB 1344276 A GB1344276 A GB 1344276A GB 1525535 A GB1525535 A GB 1525535A
Authority
GB
United Kingdom
Prior art keywords
energy
energy source
culture
supplied
source
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB13442/76A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Imperial Chemical Industries Ltd
Original Assignee
Imperial Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Imperial Chemical Industries Ltd filed Critical Imperial Chemical Industries Ltd
Priority to GB13442/76A priority Critical patent/GB1525535A/en
Priority to NO77771104A priority patent/NO150888C/en
Priority to NZ183731A priority patent/NZ183731A/en
Priority to IE661/77A priority patent/IE45274B1/en
Priority to ZA00771910A priority patent/ZA771910B/en
Priority to IN484/CAL/77A priority patent/IN156056B/en
Priority to DK143477A priority patent/DK156074C/en
Priority to SE7703773A priority patent/SE432439B/en
Priority to CA275,323A priority patent/CA1092039A/en
Priority to BE176377A priority patent/BE853186A/en
Priority to NL7703573A priority patent/NL7703573A/en
Priority to PL19713677A priority patent/PL197136A1/en
Priority to AU23859/77A priority patent/AU516883B2/en
Priority to IT7722044A priority patent/IT1114402B/en
Priority to ES457442A priority patent/ES457442A1/en
Priority to BR2085/77A priority patent/BR7702085A/en
Priority to FR7709944A priority patent/FR2430975A1/en
Priority to DE19772714708 priority patent/DE2714708A1/en
Priority to PT66391A priority patent/PT66391B/en
Priority to JP52038028A priority patent/JPS5934109B2/en
Priority to PH19626A priority patent/PH14144A/en
Priority to LU77063A priority patent/LU77063A1/xx
Priority to TR19495A priority patent/TR19495A/en
Priority to AT0234777A priority patent/AT364337B/en
Priority to CH420477A priority patent/CH637424A5/en
Priority to IL7751822A priority patent/IL51822A/en
Publication of GB1525535A publication Critical patent/GB1525535A/en
Priority to US06/069,501 priority patent/US4306026A/en
Priority to FR8000403A priority patent/FR2438683A1/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/08—Chemical, biochemical or biological means, e.g. plasma jet, co-culture
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/32—Processes using, or culture media containing, lower alkanols, i.e. C1 to C6
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes

Abstract

1525535 Regulating fermentations; tissue culture; methanol-utilizing bacteria IMPERIAL CHEMICAL INDUSTRIES Ltd 23 March 1977 [2 April 1976 8 Dec 1976] 13442/76 and 51198/76 Heading C6F The invention is based on the theory that where growing organisms are supplied with a carbon and/ or energy source such that periods of energy starvation occur, when energy is supplied to the cells after a period of energy starvation a proportion of that energy supplied is initially used by the cells in a period of adaptation to a growing state and is therefore effectively not available for efficient growth. Cells subjected to a truly continuous supply of an energy source (such as a carbon source) grow in a truly steady state where metabolite pools and enzyme activities are at effectively constant levels commensurate with efficient biomass formation. Such cultures can be made inefficient by supplying the energy source in pulses separated by time intervals. Fig. 2 depicts graphs of biomass efficiency ratio (ordinate) i.e. the ratio of the observed energy conversion efficiency of a biological system, obtained in an environment subjected to an intermittent availability of energy, to the energy conversion efficiency of a similar system subjected to continuous availability of energy source, versus cycle time (abscissa-log scale) i.e. pulse time plus the following time interval during which no exogenous energy is available to the culture before the next energy supply, for various values of the ratio Á/Á max Á being the imposed overall specific growth rate and Á max the maximum specific growth rate attainable by that culture when unlimited by the availability of carbon and energy source. The specification details suitable values of the ratio Á/Á max in relation to cycle times for various types of fermentations. Broadly, brief cycle times are best suited for processes involving the production of single cell protein and extended cycle times for the production of extracellular and intracellular metabolites e.g. citric acid, amino acids and antibiotic substances. Intermediate values corresponding to the 'dip' in the graphs (reduced efficiency) are more appropriate to the biological treatment of waste-water where it is desirable that sludge production should be reduced to a minimum, aerobic processes producing CO 2 and the microbiological production of simple organic molecules (specified). The energy source may be the carbon substrate, a light source in the case of photosynthetic reactions, hydrogen in cultures which can utilize hydrogen as an energy source and other reduced inorganic energy sources with chemolithotropic micro-organisms. The energy may be supplied to a culture, for example in a tank fermenter, in pulses separated by intervals of time, regularly or irregularly. Alternatively, there may be spatial intervals between successive energy supply points to a culture flowing along a pathway defined by physical constraints, the energy being supplied continuously or in pulses separated by intervals of time at the spaced supply points. The process is applicable to the culturing of bacteria, yeasts, fungi, and also tissue cells. The Examples are directed to the regulated cultivation of strains of Methylophilus methylotrophus (previously known as Pseudomonas methylotropha) upon methanol substrates.
GB13442/76A 1976-04-02 1976-04-02 Process for culturing cells Expired GB1525535A (en)

Priority Applications (28)

Application Number Priority Date Filing Date Title
GB13442/76A GB1525535A (en) 1976-04-02 1976-04-02 Process for culturing cells
NO77771104A NO150888C (en) 1976-04-02 1977-03-29 PROCEDURE FOR THE PREPARATION OF AN ENCELLE PROTEIN
NZ183731A NZ183731A (en) 1976-04-02 1977-03-29 Process for culturing cells(microorganism and tissue culture) dependent on the relationship between cycle time and biomass efficiency ratio
IE661/77A IE45274B1 (en) 1976-04-02 1977-03-29 Process for culturing cells
ZA00771910A ZA771910B (en) 1976-04-02 1977-03-29 Process for culturing cells
IN484/CAL/77A IN156056B (en) 1976-04-02 1977-03-30
DK143477A DK156074C (en) 1976-04-02 1977-03-31 PROCEDURE FOR THE PREPARATION OF SINGLE CELL PROTEIN
SE7703773A SE432439B (en) 1976-04-02 1977-03-31 PROCEDURE FOR CULTIVATION OF SINGLE CELL PROTEIN WITH PULSED SUPPLY OF A COLLECTION CELL
IT7722044A IT1114402B (en) 1976-04-02 1977-04-01 CELL CULTURE PROCEDURE
BE176377A BE853186A (en) 1976-04-02 1977-04-01 CELL CULTURE PROCESS
NL7703573A NL7703573A (en) 1976-04-02 1977-04-01 METHOD FOR GROWING CELLS.
PL19713677A PL197136A1 (en) 1976-04-02 1977-04-01 THE METHOD OF CULTIVATION OF CELLS
AU23859/77A AU516883B2 (en) 1976-04-02 1977-04-01 A method culturing methylophilus
CA275,323A CA1092039A (en) 1976-04-02 1977-04-01 Process for culturing cells
ES457442A ES457442A1 (en) 1976-04-02 1977-04-01 Process for culturing cells
BR2085/77A BR7702085A (en) 1976-04-02 1977-04-01 PROCESS FOR CULTIVATING CELLS PROCESS FOR THE BIOLOGICAL TREATMENT OF LIQUID MATERIAL CONTAINING BIOLOGICALLY DEGRADABLE MATERIAL FERMENTATION PROCESS PROCESS TO PRODUCE A SOLVENT
FR7709944A FR2430975A1 (en) 1976-04-02 1977-04-01 CELL CULTURE PROCESS
DE19772714708 DE2714708A1 (en) 1976-04-02 1977-04-01 METHOD OF GROWING CELLS
JP52038028A JPS5934109B2 (en) 1976-04-02 1977-04-02 Cell culture method
PT66391A PT66391B (en) 1976-04-02 1977-04-02 Process for culturing cells
PH19626A PH14144A (en) 1976-04-02 1977-04-04 Process for culturing cells
LU77063A LU77063A1 (en) 1976-04-02 1977-04-04
TR19495A TR19495A (en) 1976-04-02 1977-04-04 PROCEDURE FOR MAKING HUECRE KUELTUERUE
AT0234777A AT364337B (en) 1976-04-02 1977-04-04 METHOD FOR BREEDING CELLS
CH420477A CH637424A5 (en) 1976-04-02 1977-04-04 METHOD FOR BREEDING CELLS.
IL7751822A IL51822A (en) 1976-04-02 1977-04-05 Process for culturing cells
US06/069,501 US4306026A (en) 1976-04-02 1979-08-24 Process for culturing cells
FR8000403A FR2438683A1 (en) 1976-04-02 1980-01-09 METHOD FOR THE BIOLOGICAL TREATMENT OF A LIQUID CONTAINING A BIODEGRADABLE MATERIAL

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB13442/76A GB1525535A (en) 1976-04-02 1976-04-02 Process for culturing cells

Publications (1)

Publication Number Publication Date
GB1525535A true GB1525535A (en) 1978-09-20

Family

ID=10023077

Family Applications (1)

Application Number Title Priority Date Filing Date
GB13442/76A Expired GB1525535A (en) 1976-04-02 1976-04-02 Process for culturing cells

Country Status (3)

Country Link
BE (1) BE853186A (en)
GB (1) GB1525535A (en)
ZA (1) ZA771910B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0263900A1 (en) * 1986-10-06 1988-04-20 Celgene Corporation Process for culturing methylophilus methylotrophus

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0263900A1 (en) * 1986-10-06 1988-04-20 Celgene Corporation Process for culturing methylophilus methylotrophus

Also Published As

Publication number Publication date
BE853186A (en) 1977-10-03
ZA771910B (en) 1978-03-29

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Legal Events

Date Code Title Description
PS Patent sealed
PE20 Patent expired after termination of 20 years

Effective date: 19970322